Cone-beam computed tomographic evaluation of the temporomandibular joint and dental characteristics of patients with Class II subdivision malocclusion and asymmetry

Mingna Huang,Yun Hu,Jinfeng Yu,Jicheng Sun,Ye Ming,Leilei Zheng 대한치과교정학회 2017 대한치과교정학회지 Vol.47 No.5

Objective: Treating Class II subdivision malocclusion with asymmetry has been a challenge for orthodontists because of the complicated characteristics of asymmetry. This study aimed to explore the characteristics of dental and skeletal asymmetry in Class II subdivision malocclusion, and to assess the relationship between the condyle-glenoid fossa and first molar. Methods: Conebeam computed tomographic images of 32 patients with Class II subdivision malocclusion were three-dimensionally reconstructed using the Mimics software. Forty-five anatomic landmarks on the reconstructed structures were selected and 27 linear and angular measurements were performed. Paired-samples t -tests were used to compare the average differences between the Class I and Class II sides; Pearson correlation coefficient (r) was used for analyzing the linear association. Results: The faciolingual crown angulation of the mandibular first molar (p < 0.05), sagittal position of the maxillary and mandibular first molars (p < 0.01), condylar head height (p < 0.01), condylar process height (p < 0.05), and angle of the posterior wall of the articular tubercle and coronal position of the glenoid fossa (p < 0.01) were significantly different between the two sides. The morphology and position of the condyle-glenoid fossa significantly correlated with the three-dimensional changes in the first molar. Conclusions: Asymmetry in the sagittal position of the maxillary and mandibular first molars between the two sides and significant lingual inclination of the mandibular first molar on the Class II side were the dental characteristics of Class II subdivision malocclusion. Condylar morphology and glenoid fossa position asymmetries were the major components of skeletal asymmetry and were well correlated with the three-dimensional position of the first molar.

Long-term continuously monocropped peanut significantly disturbed the balance of soil fungal communities

Chen Mingna,Zhang Jiancheng,Liu Hu,Wang Mian,Pan LiJuan,Chen Na,Wang Tong,Jing Yu,Chi Xiaoyuan,Du Binghai 한국미생물학회 2020 The journal of microbiology Vol.58 No.7

Balancing soil microbial diversity and abundance is critical to sustaining soil health, and understanding the dynamics of soil microbes in a monocropping system can help determine how continuous monocropping practices induce soil sickness mediated by microorganisms. This study used previously constructed gradient continuous monocropping plots and four varieties with different monocropping responses were investigated. The feedback responses of their soil fungal communities to short-term and long-term continuous monocropping were tracked using high-throughput sequencing techniques. The analyses indicated that soil samples from 1 and 2 year monocropped plots were grouped into one class, and samples from the 11 and 12 year plots were grouped into another, regardless of variety. At the species level, the F. solani, Fusarium oxysporum, Neocosmospora striata, Acrophialophora levis, Aspergillus niger, Aspergillus corrugatus, Thielavia hyrcaniae, Emericellopsis minima, and Scedosporium aurantiacum taxa showed significantly increased abundances in the long-term monocropping libraries compared to the short-term cropping libraries. In contrast, Talaromyces flavus, Talaromyces purpureogenus, Mortierella alpina, Paranamyces uniporus, and Volutella citrinella decreased in the long-term monocropping libraries compared to the shortterm libraries. This study, combined with our previous study, showed that fungal community structure was significantly affected by the length of the monocropping period, but peanut variety and growth stages were less important. The increase in pathogen abundances and the decrease in beneficial fungi abundances seem to be the main cause for the yield decline and poor growth of long-term monocultured peanut. Simplification of fungal community diversity could also contribute to peanut soil sickness under long-term monocropping. Additionally, the different responses of peanut varieties to monocropping may be related to variations in their microbial community structure.

Influence of Peanut Cultivars and Environmental Conditions on the Diversity and Community Composition of Pod Rot Soil Fungi in China

( Mian Wang ),( Mingna Chen ),( Zhen Yang ),( Na Chen ),( Xiaoyuan Chi ),( Lijuan Pan ),( Tong Wang ),( Shanlin Yu ),( Xingqi Guo ) 한국균학회 2017 Mycobiology Vol.45 No.4

Peanut yield and quality are seriously affected by pod rot pathogens worldwide, especially in China in recent years. The goals of this study are to analyze the structure of fungal communities of peanut pod rot in soil in three peanut cultivars and the correlation of pod rot with environmental variables using 454 pyrosequencing. A total of 46,723 internal transcribed spacer high-quality sequences were obtained and grouped into 1,706 operational taxonomic units at the 97% similarity cut-off level. The coverage, rank abundance, and the Chao 1 and Shannon diversity indices of the operational taxonomic units were analyzed. Members of the phylum Ascomycota were dominant, such as Fusarium, Chaetomium, Alternaria, and Sordariomycetes, followed by Basidiomycota. The results of the heatmap and redundancy analysis revealed significant variation in the composition of the fungal community among the three cultivar samples. The environmental conditions in different peanut cultivars may also influence on the structure of the fungal community. The results of this study suggest that the causal agent of peanut pod rot may be more complex, and cultivars and environmental conditions are both important contributors to the community structure of peanut pod rot fungi.

Detent Force Analysis in Permanent Magnet Linear Synchronous Motor Considering Longitudinal End Effects

Li, Liyi,Ma, Mingna,Chan, C.C. Journal of International Conference on Electrical 2013 Journal of international Conference on Electrical Vol.2 No.1

This paper presents a uniform analytical model by energy method and Fourier series expansion to analyze detent force in uneven magnetic field for permanent magnet linear synchronous motor (PMLSM). The model reveals that detent force in long-primary type is mainly influenced by non-ideal distribution of permanent magnet magnetic motive force, while nounified air-gap permeance makes a great impact on detent force of short-primary type. Hence, magnetic field similarity of motor design techniques referring rotary counterpart are adopted. For long-primary type novel method of splitting edge magnets is proposed to reduce end effects force, and optimal widths of edge tooth in short-primary type also verify the effectiveness of magnetic field similarity. The experimental results validate finite element analysis results.

Influence of Peanut Cultivars and Environmental Conditions on the Diversity and Community Composition of Pod Rot Soil Fungi in China

Wang, Mian,Chen, Mingna,Yang, Zhen,Chen, Na,Chi, Xiaoyuan,Pan, Lijuan,Wang, Tong,Yu, Shanlin,Guo, Xingqi The Korean Society of Mycology 2017 Mycobiology Vol.45 No.4

Peanut yield and quality are seriously affected by pod rot pathogens worldwide, especially in China in recent years. The goals of this study are to analyze the structure of fungal communities of peanut pod rot in soil in three peanut cultivars and the correlation of pod rot with environmental variables using 454 pyrosequencing. A total of 46,723 internal transcribed spacer high-quality sequences were obtained and grouped into 1,706 operational taxonomic units at the 97% similarity cut-off level. The coverage, rank abundance, and the Chao 1 and Shannon diversity indices of the operational taxonomic units were analyzed. Members of the phylum Ascomycota were dominant, such as Fusarium, Chaetomium, Alternaria, and Sordariomycetes, followed by Basidiomycota. The results of the heatmap and redundancy analysis revealed significant variation in the composition of the fungal community among the three cultivar samples. The environmental conditions in different peanut cultivars may also influence on the structure of the fungal community. The results of this study suggest that the causal agent of peanut pod rot may be more complex, and cultivars and environmental conditions are both important contributors to the community structure of peanut pod rot fungi.

Detection of copy number variation and selection signatures on the X chromosome in Chinese indigenous sheep with different types of tail

Zhu Caiye,Li Mingna,Qin Shizhen,Zhao Fuping,Fang Suli 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.9

Objective: Chinese indigenous sheep breeds can be classified into the following three categories by their tail morphology: fat-tailed, fat-rumped and thin-tailed sheep. The typical sheep breeds corresponding to fat-tailed, fat-rumped, and thin-tailed sheep are large-tailed Han, Altay, and Tibetan sheep, respectively. Detection of copy number variation (CNV) and selection signatures provides information on the genetic mechanisms underlying the phenotypic differences of the different sheep types. Methods: In this study, PennCNV software and F-statistics (FST) were implemented to detect CNV and selection signatures, respectively, on the X chromosome in three Chinese indigenous sheep breeds using ovine high-density 600K single nucleotide polymorphism arrays. Results: In large-tailed Han, Altay, and Tibetan sheep, respectively, a total of six, four and 22 CNV regions (CNVRs) with lengths of 1.23, 0.93, and 7.02 Mb were identified on the X chromosome. In addition, 49, 34, and 55 candidate selection regions with respective lengths of 27.49, 16.47, and 25.42 Mb were identified in large-tailed Han, Altay, and Tibetan sheep, respectively. The bioinformatics analysis results indicated several genes in these regions were associated with fat, including dehydrogenase/reductase X-linked, calcium voltage-gated channel subunit alpha1 F, and patatin like phospholipase domain containing 4. In addition, three other genes were identified from this analysis: the family with sequence similarity 58 member A gene was associated with energy metabolism, the serine/arginine-rich protein specific kinase 3 gene was associated with skeletal muscle development, and the interleukin 2 receptor subunit gamma gene was associated with the immune system. Conclusion: The results of this study indicated CNVRs and selection regions on the X chromosome of Chinese indigenous sheep contained several genes associated with various heritable traits.

Molecular and Cellular Microbiology / Biomedical Sciences : A Homogeneous Immunoassay Method for Detecting Interferon-Gamma in Patients with Latent Tuberculosis Infection

( Fei Wu ),( Lin Wang ),( Qiaomei Guo ),( Mingna Zhao ),( Hongchen Gu ),( Hong Xu ),( Jiatao Lou ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.3

IFN-γ release assays (IGRAs) have been developed as viable alternative diagnostic tools for detecting latent tuberculosis infection (LTBI). A customized homogeneous sandwich luminescent oxygen channeling immunoassay (LOCI) was used to quantify IFN-γ levels in IGRAs. Samples were collected from healthy volunteers (n = 40) who were T-Spot-negative and T-Spot-positive patients (n = 32) at rest. Then the amount of IFN-γ in the supernatant of IGRAs was measured by LOCI. The results demonstrated a low background, and high sensitivity, specificity, accuracy, and reproducibility, and a short assay time (only 30 min) with LOCI for IFN-γ. The recovery range was 81.63-102.06%, the coefficients of variation were below 5%, and the limit of detection was 19.0 mIU/ml. Excellent agreement between LOCI IFN-γ and the T-SPOT.TB test was obtained (97.2% agreement, κ = 0.94). The LOCI IFN-γ concentrations were significantly higher in T-Spot-positive patients than in the healthy group (p < 0.001). Moreover, as observed for the comparative LOCI IFN-γ assay, IFN-γ concentrations were related to the numbers of T-SPOT.TB spots. We have established an in vitro blood test for LTBI diagnosis, defined as LOCI IFN-γ. A high level of agreement between the LOCI IFN-γ method and T-SPOT.TB assay was observed in clinical studies that showed the LOCI IFN-γ method could determine LTBI. This study shows acceptable performance characteristics of the LOCI IFN-γ assay to diagnose LTBI.

Analysis and Optimization of Air-Core Permanent Magnet Linear Synchronous Motors with Overlapping Concentrated Windings for Ultra-precision Applications

Li, Liyi,Tang, Yongbin,Ma, Mingna,Pan, Donghua Journal of International Conference on Electrical 2013 Journal of international Conference on Electrical Vol.2 No.1

This paper presents the analysis and optimization of air-core permanent magnet linear synchronous motor with overlapping concentrated windings to achieve high thrust density, high thrust per copper losses and low thrust ripple. For the motor design, we adopt equivalent magnetizing current (EMC) method to analyze the magnetic field and give analytical formulae for calculation of motor parameters such as no-load back EMF, dynamic force, thrust density and thrust per copper losses. Further, we proposed a multi-objective optimization by genetic algorithm to search for the optimum parameters. The design optimization is verified by 2-D Finite Element analysis (FEA).

Research and Development of Superhard Cutting Database

HuiPing Zhang,WenJuan Zheng,YiNan Lai,Mingna Ding,Zhen Chen 보안공학연구지원센터 2014 International Journal of Hybrid Information Techno Vol.7 No.5

Transcriptome analysis reveals salt-stress-regulated biological processes and key pathways in roots of peanut (Arachis hypogaea L.)

Shanlin Yu,Na Chen,Maowen Su,Xiaoyuan Chi,Zhimeng Zhang,Lijuan Pan,Mingna Chen,Tong Wang,Mian Wang,Zhen Yang 한국유전학회 2016 Genes & Genomics Vol.38 No.6

The cultivated peanut is important oil crop and salt stress seriously influences its development and yield. Tolerant varieties produced using transgenic techniques can effectively increase peanut plantation area and enhance its yields. However, little is known about how gene expression is regulated by salt stress in peanut. In this study, we screened genes regulated by salt stress in peanut roots using microarray technique. In total, 4828 up-regulated and 3752 down-regulated probe sets were successfully identified in peanut roots subjected to 3 and 48 h of salt stress. Data analysis revealed that different response groups existed between the up and down-regulated probe sets. The main up-regulated biological processes involved in salt stress responses included transcription regulation, stress response, and metabolism and biosynthetic processes. The main down-regulated biological processes included transport processes, photosynthesis and development. The Kyoto encyclopedia of genes and genomes pathway analysis indicated that metabolic pathway, biosynthesis of unsaturated fatty acids and plant–pathogen interaction, were mainly up-regulated in peanut under salt stress. However, photosynthesis and phenylalanine metabolism were mainly down-regulated during salt stress. The function of some probe sets in salt stress regulation was not clarified (e.g., protein functioning in cell cycle regulation and xylem development). Many of the genes we identified lacked functional annotations and their roles in response to salt stress are yet to be elucidated. These results identified some candidate genes as potential markers and showed an overview of the transcription map, which may yield some useful insights into salt-mediated signal transduction pathways in peanut.