Uniaxial Compression Properties and Compression Fatigue Performance of Selective Laser Melted Ti–6Al–4V Cellular Structure

Ming‑Wei Wu,Jhewn‑Kuang Chen,Mo‑Kai Tsai,Pei Wang,Tien‑Lin Cheng,Bo‑Huan Lin,Po‑Hsing Chiang,Annadurai Dhinakar 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.1

The selective laser melted (SLM) Ti–6Al–4V cellular solid is a promising material in biomedical and other applications. The mechanical properties of the SLM Ti–6Al–4V cellular material are predominantly controlled by the design of the cellular structure. The objective of this study was to investigate the uniaxial compressive properties and compressive fatigueperformance of the SLM Ti–6Al–4V cellular solid with a new cuboctahedron unit cell reinforced by four diagonal strutsand two horizontal struts along the X and Y axes. The infuences of hot isostatic pressing (HIP) on the various mechanicalproperties were also examined. The results showed a smoother stress–strain curve and fewer stress oscillations in the HIPspecimen than in the SLM specimen. The HIP process slightly decreased the yield strength of the SLM sample from 55 to52 MPa. Under uniaxial compressive stress, the SLM and HIP specimens exhibited layer-by-layer fracture and diagonalshear fracture, respectively. Furthermore, the HIP treatment signifcantly increased the fatigue performance and improved thefatigue endurance ratio at 106cycles from 0.15 to 0.35. The HIP treatment contributed to several positive efects, includingphase transformation from brittle α′ to α + β, the elimination of strut porosity and residual stress, an increase in lath size,and a better combination of strength and ductility, thereby improving the fatigue performance. The efects of stress mode onthe fracture mechanism were also clarifed using in situ observation in combination with digital image correlation.

Applying Physiological Status Monitoring in Improving Construction Safety Management

Ming-Kuan Tsai 대한토목학회 2017 KSCE JOURNAL OF CIVIL ENGINEERING Vol.21 No.6

A variety of research has highlighted the importance of improving safety in the construction industry. Due to the attributes of construction activities (e.g., inappropriate shiftwork and schedules) and sites (e.g., darkness, narrowness), human errors frequently cause construction accidents, especially when construction workers suffer from mental and/or physical fatigue. To address this problem, this study proposes an approach based on Physiological Status Monitoring (PSM). By detecting brain wave rhythms and Heart Rate Variability (HRV) of workers, the proposed approach could analyze fatigue levels. After identifying risks of fatigue, the proposed approach notifies the fatigued workers as well as transfers relevant statistics to construction managers. The managers, therefore, are able to supervise their workers in real time. Given the results of on-site tests, construction safety management is enhanced, while the construction accidents caused by mentally and/or physically fatigued workers are circumvented as quickly as possible. Consequently, this study is a useful reference for similar applications in many countries.

Hospitality and Culture in the Smile

( Tsai Ming Chun ) 한국기초조형학회 2019 한국기초조형학회 작품집 Vol.2019 No.1

Cooperative emergency braking warning system in vehicular networks

Tsai, Ming-Fong,Chao, Yung-Cheng,Chen, Lien-Wu,Chilamkurti, Naveen,Rho, Seungmin SpringerOpen 2015 Eurasip Journal on Wireless Communications and Net Vol.2015 No.-

Critical Reflections on Abiding-Places in the Perfection of Wisdom in Eight Thousand Lines

TSAI Yao-ming 동국대학교 불교학술원 2021 International Journal of Buddhist Thought & Cultur Vol.31 No.2

This article focuses on the real abiding of the mind-body complex demonstrated in the Aṣṭasāhasrikā Prajnāpāramitā with an emphasis on how Buddhist teachings explore the notion of abiding-places or temporary residences in one lifetime. The key findings of this article can be summarized in the following key points. First, any abiding-place enduring for long lifespans, including those temporary residences in one lifetime, are impermanent and devoid of own-being. Second, in terms of an abiding-place per se, none of the abiding-place is qualified to be real abiding-place for the mind-body complex due to the lack of own-being. Third, the misconception that the mind-body complex can permanently settle in a fixed abiding-place not only leads to emotional fluctuations, but also lays a preposterous emphasis on environmental surroundings. Fourth, bodhisattvas cultivate prajnāpāramitā so their mind-body complexes can abide rightly. Fifth, bodhisattvas channel sentient beings to the real abiding of the mind-body complex through helping sentient beings relieve suffering and abide in any of the Three Paths of cultivation, i.e., śrāvakayāna, pratyekabuddhayāna, and bodhisattvayāna.

Nerve growth factor upregulates sirtuin 1 expression in cholestasis: a potential therapeutic target

Ming-Shian Tsai,Po-Huang Lee,Cheuk-Kwan Sun,Ting-Chia Chiu,Yu-Chun Lin,I-Wei Chang,Po-Han Chen,Ying-Hsien Kao 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

This study investigated the regulatory role of nerve growth factor (NGF) in sirtuin 1 (SIRT1) expression in cholestatic livers. We evaluated the expression of NGF and its cognate receptors in human livers with hepatolithiasis and the effects of NGF therapy on liver injury and hepatic SIRT1 expression in a bile duct ligation (BDL) mouse model. Histopathological and molecular analyses showed that the hepatocytes of human diseased livers expressed NGF, proNGF (a precursor of NGF), TrkA and p75NTR, whereas only p75NTR was upregulated in hepatolithiasis, compared with non-hepatolithiasis livers. In the BDL model without NGF therapy, p75NTR, but not TrkA antagonism, significantly deteriorated BDL-induced liver injury. By contrast, the hepatoprotective effect of NGF was abrogated only by TrkA and not by p75NTR antagonism in animals receiving NGF therapy. Intriguingly, a positive correlation between hepatic SIRT1 and NGF expression was found in human livers. In vitro studies demonstrated that NGF upregulated SIRT1 expression in mouse livers and human Huh-7 and rodent hepatocytes. Both NGF and proNGF induced protective effects against hydrogen peroxide-induced cytotoxicity in Huh-7 cells, whereas inhibition of TrkA and p75NTR activity prevented oxidative cell death. Mechanistically, NGF, but not proNGF, upregulated SIRT1 expression in human Huh-7 and rodent hepatocytes via nuclear factor (NF)-κB activity, whereas NGF-induced phosphoinositide-3 kinase/Akt, extracellular signal–regulated kinase and NF-κB signaling and SIRT1 activity were involved in its hepatoprotective effects against oxidative injury. These findings suggest that pharmacological manipulation of the NGF/SIRT1 axis might serve as a novel approach for the treatment of cholestatic disease.

Study on a Binder by Using Porcine Blood Plasma Transglutaminase, Thrombin and Fibrinogen

Tsai, Chong-Ming,Tseng, Tsai-Fuh,Yang, Jeng-Huh,Chen, Ming-Tsao Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.1

The purpose of this study was to prepare a binder containing porcine blood transglutaminase (TGase), thrombin and fibrinogen. Extracted TGase, thrombin and fibrinogen were used alone or mixed with different proportions of their volume (v/v/v) by nine combinations as follows were 0.5:1:15, 0.5:1:20, 0.5:1:25, 1:1:15, 1:1:20, 1:1:25, 1.5:1:15, 1.5:1:20 and 1.5:1:25, respectively. Five ml of each combination were mixed with 0.6 ml of 0.25 M calcium chloride before experiment. After storage at 4C for 0, 1, 2, 3, 4 and 5 weeks, enzyme activity, total plate count, pH value, and SDS-PAGE of TGase, thrombin and fibrinogen were tested and pH value, clotting time and gel strength of the nine combination binders were determined. The results showed that total plate count of thrombin and pH value of TGase were significantly higher (p<0.05) than in other treatments. SDS-PAGE results showed that purified TGase, thrombin and fibrinogen from porcine blood plasma compared with commercial products (Sigma) had the same band patterns and nine different combination binders had no significant effect. Enzymatic activity of TGase and thrombin decreased as storage time increased. Total plate count of TGase, thrombin and fibrinogen and clotting time of the binder increased as storage time increased. The higher amount of fibrinogen in combinations, the stronger the gel strength.

A Contemporary Application of Buddhist Worldview for Understanding and Transforming Sentient Beings

TSAI Yao-ming 동국대학교 불교학술원 2016 International Journal of Buddhist Thought & Cultur Vol.26 No.1

Based on the Anūnatvâpūrṇatva-nirdeśa and the discourse on worldviews therein,this paper examines the migration among abiding-places and the relationship betweensentient beings and worldly abiding-places from the viewpoint of cyclic rebirth(saṃsāra). Foregrounding worldviews as its discursive landscape and acknowledgingthe diversity thereof in this world, this paper further discusses the challenges posed by theexperience of different, and sometimes antagonistic, worldviews from the perspective ofco-arising and mutual accomplishment to exemplify the inclusive characteristic of theAnūnatvâpūrṇatva-nirdeśa. The Anūnatvâpūrṇatva-nirdeśa breaks down sentient beings’ repeated births anddeaths into the realm of sentient beings, eka-dharma-dhātu, and tathāgata-garbha,all of which are not only rooted in dharma-kāya, but, in their relation to the realm ofsentient beings, also point the way to life-practice. Through life-practice, the sufferingof repeated births and deaths is clearly recognized, and hence one had better not cling toany worldly abiding-places. By examining into the facts connected with cyclic rebirth,one can cultivate remarkable ability to detach oneself from any investment in worldlyabiding-places and thus become liberated and at ease. In addition to an examination of the abiding-places, the issue of how variousworldviews should be cognized will also be explored. From the vantage point ofeka-dharma-dhātu, first, the Anūnatvâpūrṇatva-nirdeśa examines the variety ofworldviews from the angle of conditioned co-arising. Second, it channels those whofirmly hold their preconceptions to mutual growth through its inclusive characteristic.

Upregulating sirtuin 6 ameliorates glycolysis, EMT and distant metastasis of pancreatic adenocarcinoma with krüppel-like factor 10 deficiency

Tsai Yi-Chih,Chen Su-Liang,Peng Shu-Ling,Tsai Ya-Li,Chang Zuong-Ming,Chang Vincent Hung-Shu,Ch’ang Hui-Ju 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

Krüppel-like factor 10 (KLF10) is a tumor suppressor in multiple cancers. In a murine model of spontaneous pancreatic adenocarcinoma (PDAC), additional KLF10 depletion accelerated distant metastasis. However, Klf10 knockout mice, which suffer from metabolic disorders, do not develop malignancy. The mechanisms of KLF10 in PDAC progression deserve further exploration. KLF10-depleted and KLF10-overexpressing PDAC cells were established to measure epithelial-mesenchymal transition (EMT), glycolysis, and migration ability. A murine model was established to evaluate the benefit of genetic or pharmacological manipulation in KLF10-depleted PDAC cells (PDACshKLF10). Correlations of KLF10 deficiency with rapid metastasis, elevated EMT, and glycolysis were demonstrated in resected PDAC tissues, in vitro assays, and murine models. We identified sirtuin 6 (SIRT6) as an essential mediator of KLF10 that modulates EMT and glucose homeostasis. Overexpressing SIRT6 reversed the migratory and glycolytic phenotypes of PDACshKLF10 cells. Linoleic acid, a polyunsaturated essential fatty acid, upregulated SIRT6 and prolonged the survival of mice injected with PDACshKLF10. Modulating HIF1α and NFκB revealed that EMT and glycolysis in PDAC cells were coordinately regulated upstream by KLF10/SIRT6 signaling. Our study demonstrated a novel KLF10/SIRT6 pathway that modulated EMT and glycolysis coordinately via NFκB and HIF1α. Activation of KLF10/SIRT6 signaling ameliorated the distant progression of PDAC. Clinical Trial Registration: ClinicalTrials.gov. identifier: NCT01666184.

INVESTIGATING SATISFIED BUT DISLOYAL BUSINESS CUSTOMERS

Ming-Chih Tsai,Rico Merkert,Shiau-Chi Lin,Mei-Ting Tsai 글로벌지식마케팅경영학회 2018 Global Marketing Conference Vol.2018 No.07

Satisfaction paradox indicates customer satisfaction does not necessarily translate into customer repurchase. Competitor’s attractiveness and customer demographics are two main moderators for the paradoxical phenomenon. Competitor’s attractiveness exhibits customer switching effect, whereas customer demographics imply customers moored with their current service provider. Still, the cases were few investigated in B2B settings where customers are in general more complex than consumers as involving more intricate organizational network influences. In a business network, transactions may incur buyer/seller relational activities that mitigate paradoxical behavior. Nonetheless, the variable is yet identified and the interacting effects between the moderating variables are not clarified. Thus, this study aims to develop an operational model to classify satisfied customers into loyal, moored and paradoxical segments using three genres of moderators: competitor’s attractiveness, organizational and transactional variables in a B2B setting. In the model, the theoretical bases of switching behaviors are applied for four statistical analysis executed in a logic sequence, including a factor analysis to consolidate quality measurements, a quadrant analysis to locate the effect of competitor’s attractiveness, a clustering analysis to segment satisfied customers into four segments, and finally, nonparametric tests to validate the organizational and transactional segmentation variables. Empirically, we study Taiwanese manufacturers who engaged in global trade and have had experiences in choosing the global air express services that form an oligopolistic market and a strategic group so competitor’s attractiveness can be better calibrated. A total of 180 valid samples are collected and analyzed. The results contribute to the literatures of B2B service marketing.