HMGB1 regulates autophagy through increasing transcriptional activities of JNK and ERK in human myeloid Leukemia cells

( Ming Yi Zhao ),( Ming Hua Yang ),( Liang Chun Yang ),( Yan Yu ),( Min Xie ),( Shan Zhu ),( Rui Kang ),( Dao Lin Tang ),( Zhi Gang Jiang ),( Wu Zhou Yuan ),( Xiu Shan Wu ),( Li Zhi Cao ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.9

HMGB1 is associated with human cancers and is an activator of autophagy which mediates chemotherapy resistance. We here show that the mRNA levels of HMGB1 are high in leukemia cells and it is involved in the progression of childhood chronic myeloid leukemia (CML). HMGB1 decreases the sensitivity of human myeloid leukemia cells K562 to anti-cancer drug induced death through up-regulating the autophagy pathway, which is confirmed by the observation with an increase in fusion of autophagosomes and autophagolysosomes. When overexpressing HMGB1, both mRNA levels of Beclin-1, VSP34 and UVRAG which are key genes involved in mammalian autophagy and protein levels of p-Bcl-2 and LC3-II are increased. Luciferase assays document that over-expression of HMGB1 increases the transcriptional activity of JNK and ERK, which may be silenced by siRNA. The results suggest that HMGB1 regulates JNK and ERK required for autophagy, which provides a potential drug target for therapeutic interventions in childhood CML. [BMB reports 2011; 44(9): 601-606]

Studies on the Time Stability of the Magnetic Performance of Nd-Fe-B Magnets

Zhu Ming-Gang,Liu Xing-Min,Li Zheng-bang,Li Wei 한국자기학회 2007 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

한국 영상매체 접촉과 관광지 이미지, 방문의도 간의 관계

왕명명(Ming Ming Wang),류시영(Si Young Ryu),송운강(Woon Gang Song) 한국관광연구학회 2011 관광연구저널 Vol.25 No.1

The purpose of this study is to explore the relationship between visual media`s contact and intention to visit after watching Korean dramas and movies. Korean Wave became one of the important research project in tourism field. From the literature review, the authors propose a new model of destination image formation process including the familiarity. In order to analyze the new model, data were collected from 335 students. This study carried out descriptive statistical analysis, reliability analysis, factor analysis and path analysis to test the model. The findings of this study are as follows: 1) the visual media contact frequency had positive influence on familiarity, 2) The familiarity had positive influence on cognitive image, affectional image and overall image, 3) The cognitive image had positive influence on affectional image and on overall image, and affectional image also had positive influence on overall image, 4) cognitive image, affectional image and the overall image showed the positive influence on potential tourists`s intention to visit. Based on the results of this study, several points were made from the perspectives of international tourism marketing. The limitations of the study and the future research directions are also discussed.

Preparation of Homogeneous Microstructure Pure Lead Metaniobate by Two-Step Sintering

Xiao-gang Zhao,Peng Liu,Bo Chao,Su Juan Liu,Ming Pang,Xiao-Ming Chen,Jian-Ping Zhou 대한금속·재료학회 2014 ELECTRONIC MATERIALS LETTERS Vol.10 No.1

The purpose of the present work is to obtain the Lead metaniobate ceramics with the orthorhombic phase via a two-step sintering method. The samples were first sintered at 1320°C for 10 min, and then sintered separately at 1260°C, 1220°C, and 1180°C for 4 h. All the ceramics show orthorhombic phase and homogeneous microstructure. It was found that the abnormal grain growth was restrained obviously.

가현운동을 이용한 애니메이션 장치 개발 연구

강춘명 ( Gang Chun Ming ),전신종 ( Jeon Shin Jong ) 한국기초조형학회 2018 기초조형학연구 Vol.19 No.1

인간의 시각은 본능적으로 정지된 것보다 움직이는 것에 대해 더욱 민감하게 반응한다. 따라서 과거로부터 정지된 이미지를 움직이는 것처럼 보이게 하려는 많은 노력이 있어 왔다. 그 예가 무토스코프(Mutoscope), 스트로보스코프(Stroboscope), 조트로프(Zoetrope), 스캐니메이션(Scanimation)등과 같은 가현운동 원리를 이용한 방법이다. 가현운동은 실제로 물리적인 운동은 발생하지 않은 상태이지만, 움직이는 것처럼 보이는 시지각 상의 움직임이다. 본 연구는 아나모르포즈(Anamorphose)원리와 가현운동을 결합시킨 새로운 애니메이션 장치 개발을 제시하고, 다양한 실험과 수학적 알고리즘을 통해 제작 가능성을 검증하는데 그 목적이 있다. 1장과 2장에서는 연구배경과 목적, 가현운동을 통한 다양한 애니메이션 장치에 대하여 분류 기술하였고, 3장과 4장에서는 새로운 애니메이션 장치인 스캐니마스코프(Scanimascope)의 의의와 다양한 장치 개발의 실험을 진행하였다. 이상과 같은 과정을 통해 다음과 같은 결론을 얻을 수 있었다. 첫째, 가현운동 원리를 이용한 애니메이션의 새로운 장치를 개발하는데 의의가 있으며, 이 장치의 이름을 “스캐니마스코프(Scanimascope)”로 명명하였다. 둘째, 아나모르포즈의 원리를 적용한 반사그림은 분할된 거울의 반사를 통해 그림을 모아서 보는 것으로써 주관적 윤곽선의 인지에 제한을 받는다. 따라서 반사그림의 이미지 컷이 많을 경우에는 동작의 흐름이 매끄럽지 않으므로 2~3개 그림이 합쳐지는 것이 이상적이다. 셋째, 잔상효과를 유지하기 위해서는 이미지의 동작 변환 시간은 0.2~0.4초 사이에 있어야 한다. 따라서 속도에 비례하여 거울의 너비는 1.5mm일 때 가장 효과적인 동작 이미지를 얻을 수가 있다. 스캐니마스코프(Scanimascope)는 주변 환경의 영향을 별로 받지 않으며, 설치 장소나 조건에 있어서 매우 가변적이고 효율적이기 때문에 다양한 방법으로서의 활용 가치가 있다고 판단된다. 또한, 첨단 컴퓨터의 발전이 눈부신 현대에서 스캐니마스코프(Scanimascope)의 아날로그적 원리는 새로운 영역을 창출과 더불어 다양한 분야에서의 활용 가치를 기대해 본다. As the human vision instinctively responds more sensitively to movement than still forms, endless efforts have been made to make bring movement to still images. The fruits of such efforts are mutoscope, stroboscope, zoetrope, and scanimation, etc. which are based on the principle of apparent motion. Apparent motion refers to a state which is perceived to move visually but does not actually involve physical motion. The methods devised using the principle of apparent motion hold physical limitations, such as needing a dark environment, being reproducible only on a plane, needing electricity, and requiring a fast speed. To this end, this study illustrates the development of a new animation device that combines the principles of anamorphosis and apparent motion and verifies the production possibility through various experiments and mathematical algorithms. The scope of the study was limited to experiments applying the principles of apparent motion and anamorphosis to planar mirrors. Chapters 1 and 2 describe the background and purpose of this research as well as the various types of animation devices that use apparent motion. In Chapters 3 and 4, we explain the significance of a new animation device, which we named scanimascope, and present our multiple experimentations for developing the device. Through this process, we underline the importance of developing a new device for animation using the principle of apparent motion. Compared to existing animation devices, the scanimascope is a three-dimensional structure that does not need to be viewed through a specific device and is relatively free from environmental limitations. We found that mirror anamorphosis, which puts together reflected images using multiple mirrors, relies on the individual perception of contours and therefore, too many images can prohibit the smooth flow of motion, making two or three images the ideal number. Also, to maintain the afterimage effect, the image’s motion transition time should be between 0.2 and 0.4 seconds, which means that the most effective motion image can be obtained when the width of the mirror is 1.5 mm in proportion to the speed. As it is minimally affected by the surrounding environment, the scanimascope can be usefully and efficiently installed anywhere under any condition. We hope that the scanimascope’s analog principles, which contrasts with the development of advanced information technology in the modern era, will open a new field that can be utilized in various areas.

Predictive Role of Glutathione-S-transferase Gene Polymorphisms in Risk and Prognosis of Hepatocellular Carcinoma

Li, Cheng-Gang,Zhao, Zhi-Ming,Hu, Ming-Geng,Liu, Rong Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

Aim: We conducted a prospective study in an Chinese population to detect associations of GSTM, GSTT and GSTP polymorphisms with hepatocellular carcinoma (HCC), and analyze roles in determining survival outcome. Methods: A prospective follow-up study was conducted with 476 HCC patients and 481 controls collected from May 2005 to May 2007. All patients were followed up until the end of Dec. 2011. GSTM1, GSTT1 and GSTP1 genotyping were performed by PCR-CTPP methods. Results: Null GSTM1 carriers had a 1.64 fold risk of HCC compared with non-null genotype, while GSTP1 Val/Val carriers had a 93% increased risk over the GSTP1 IIe/IIe genotype. The median follow-up time for the 476 patients was 34.2 months (range: 1 to 78 months). Individuals with null GSTM1 genotype had better survival of HCC than non-null genotype carriers (HR=0.71, 95%CI=0.45-0.95). Similarly, GSTP1 Val/Val genotypes had significant better survival than the GSTP1 IIe/IIe genotype (HR=0.34, 95%CI=0.18-0.65). Individuals carrying null GSTM1 and GSTP1 Val/Val who received chemotherapy had lower risk of death from HCC than those without chemotherapy. Conclusion: This study indicated carriage of null GSTM1 and GSTP1 Val/Val genotypes to have roles in susceptibility to and survival from HCC.

Is It Possible to Replace Microendoscopic Discectomy with Percutaneous Transforaminal Discectomy for Treatment of Lumbar Disc Herniation? A Meta-Analysis Based on Recurrence and Revision Rate

Zhao, Xiao-ming,Yuan, Qi-ling,Liu, Liang,Shi, Ya-ming,Zhang, Yin-gang The Korean Neurosurgical Society 2020 Journal of Korean neurosurgical society Vol.63 No.4

Objectives : Due to recent developments and the wide application of percutaneous transforaminal discectomy (PTED) in China, we herein compare its clinical effects with microendoscopic discectomy (MED) for the treatment of lumbar disc herniation in terms of recurrence and revision rates. Methods : Six databases, namely, PubMed, EMBASE, Cochrane Library, Ovid, China National Knowledge Infrastructure and Wanfang, were searched by computer. The literature was screened according to inclusion and exclusion criteria, and the quality of the included literature was evaluated. After extracting the data from the papers, Review Manager 5.2 software (Cochrane Collaboration, Oxford, UK) was applied to analyze these data. Finally, sensitivity and publication bias analyses of the results were conducted. Results : A total of 12 studies consisting of 2400 patients were included in this meta-analysis. A comparison of PTED with MED revealed higher postoperative recurrence and postoperative revision rates for PTED (odds ratio [OR] recurrence, 1.60; 95% confidence interval [CI], 1.01 to 2.53; p=0.05 and OR revision, 1.77; 95% CI, 1.18 to 2.64, p=0.006). Conclusion : PTED has a number of advantages because it is a minimally invasive surgery, but its recurrence and revision rates are higher than MED. Therefore, MED should not be completely replaced by PTED.

RON and MET Co-overexpression Are Significant Pathological Characteristics of Poor Survival and Therapeutic Targets of Tyrosine Kinase Inhibitors in Triple-Negative Breast Cancer

Tian-Hao Weng,Min-Ya Yao,Xiang-Ming Xu,Chen-Yu Hu,Shu-Hao Yao,Yi-Zhi Liu,Zhi-Gang Wu,Tao-Ming Tang,Pei-Fen Fu,Ming-Hai Wang,Hang-Ping Yao 대한암학회 2020 Cancer Research and Treatment Vol.52 No.3

Purpose Triple-negative breast cancer (TNBC) is highly malignant and has poor prognosis and a high mortality rate. The lack of effective therapy has spurred our investigation of new targets for treating this malignant cancer. Here, we identified RON (macrophage-stimulating 1 receptor) and MET (MET proto-oncogene, receptor tyrosine kinase) as a prognostic biomarker and therapeutic targets for potential TNBC treatment. Materials and Methods We analyzed RON and MET expression in 187 primary TNBC clinical samples with immunohistochemistry. We validated the targeted therapeutic effects of RON and MET in TNBC using three tyrosine kinase inhibitors (TKIs): BMS-777607, INCB28060, and tivantinib. The preclinical therapeutic efficacy of the TKIs was mainly estimated using a TNBC xenograft model. Results Patients with TNBC had widespread, abnormal expression of RON and MET. There was RON overexpression, MET overexpression, and RON and MET co-overexpression in 63 (33.7%), 63 (33.7%), and 43 cases (23.0%), respectively, which had poor prognosis and short survival. In vivo, the TKI targeting RON ant MET inhibited the activation of the downstream signaling molecules, inhibited TNBC cell migration and proliferation, and increased TNBC cell apoptosis; in the xenograft model, they significantly inhibited tumor growth and shrank tumor volumes. The TKI targeting RON and Met, such as BMS-777607 and tivantinib, yielded stronger anti-tumor effects than INCB28060. Conclusion RON and MET co-overexpression can be significant pathological characteristics in TNBC for poor prognosis. TKIs targeting RON and MET have stronger drug development potential for treating TNBC.

1.54 μm photoluminescence emission at room-temperature of erbium-implanted lithium niobate crystal

Gang Fu,Shi Ling Li,Xue Lin Wang,Feng Chen,Ke Ming Wang 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.52 No.-

Erbium doped materials are of great interest in integrated optoelectronic technology, due to their Er³+ intra-4f emission at 1.54 μm, a standard telecommunication wavelength. Lithium niobate crystals (LN) are of great importance for fabrication of integrated optical devices, due to their excellent properties such as high electro-optical coefficient, low propagation loss, and high Curie temperature. Lithium niobate crystal has been implanted with 500 keV Er ions at a fluence of 3.0×10 15 ions/㎝² with the aim of optically doping the material in the near surface region. In order to recrystallize the amorphized implanted region the sample was annealed at 500℃ for 90 min in oxygen atmosphere. Photoluminescence (PL) and Rutherford backscattering spectrometry studies were performed on the as-implanted sample before and after annealing. 1.54 μm room-temperature photoluminescence emission was observed in the annealed sample. The relationship between annealing temperature and photoluminescence intensity is discussed.

Experimental Investigation and Modeling on Residual Stress of Welded Steel Circular Tubes

Gang Shi,Xue Jiang,Wenjing Zhou,Tak-Ming Chan,Yong Zhang 한국강구조학회 2013 International Journal of Steel Structures Vol.13 No.3

To investigate the residual stress of welded steel circular tubes, an experimental program including 9 different sectional dimensions and different steel strength specimens were described in which sectioning method was employed. Based on large quantities of original test data, the residual stress distribution and the magnitudes of these different specimens were obtained,and the effects of the diameter to thickness ratio, the steel strength and hot galvanizing process as well as welding types were analysed. It was found that the residual stress distribution of high strength steel welded tubes was flatter than the ones of ordinary steel tubes, and galvanizing process decreased the maximum residual tensile stress. Finally, the residual stress distribution model for steel welded circular tube sections was proposed which showed good agreement with the test results. Meanwhile, the finite element verification was carried out which provided reference for the future FEA of column buckling of high strength steel circular tubes. The research results provide foundation for the future study on the column buckling of welded steel circular tubes.