자궁경부 상피종양에서 human papillomavirus 감염과 survivin 발현

윤재호,정동준,이정은,박동명,배동한,선우재근,백무준,김창진 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Human papillomavirus (HPV) has been considered a causative agent of uterine cervical carcinoma. HPV is a DNA oncogenic virus, which is well known as a causative virus in uterine cervical carcinoma. The virus is classified into two groups genotypically, low risk and high risk, according to the carcinogenic potentiality, and the determination of the viral genotype is important in clinical practice. Recently, numerous genotypes can be determined by high throughput method using DNA chip. Survivin is a recently characterized inhibitor of anti-apoptosis (IAP) protein, which is abundantly expressed in most solid and hematological malignancies, but undetectable in normal adult tissues. In this study, HPV genotypes are determined by DNA chip and the expression of survivin was examined by immunohistochemistry in 80 cases of uterine cervical intraepithelial neoplasia (CIN) and invasive carcinoma to see the roles of HPV and survivin in the carciogenesis of uterine cervical epithelial neoplasia. The results were as follows: 1. HPV positive rate was 72.5%, while negative rate was 27.5% in 80 cases of CIN and invasive carcinoma. The CIN and invasive carcinoma showed higher HPV positive rate (p <0.05). 2. HPV positive rate according to the histologic grade were 60%, 65%, 77% and 90% in CINI, CINII, CINII and invasive carcinoma, respectively. HPV positive rate showed increasing tendency according to the histologic grade, though there was no statistical significance. 3. The most frequent genotype was type 16 and the next were 58, 52, 18 and 33 in order of frequency. 4. Survivin was expressed in 96.3% of CIN and invasive carcinoma. The expression rate of survivin showed no significant difference between the histologic grade of CIN and invasive carcinoma, but showed tendency of increased expression rate in invasive carcinoma. 5. Survivin was expressed in HPV positive and in HPV negative each as in 95.5% and 96.6% respectively. There was no significant difference of survivin expression between HPV positive and negative cases. The above results suggest that HPV has no effect on the regulation of survivin expresson level in the uterine cervical intraepithelial neoplasia and invasive carcinomas.

皮下脂肪厚 測定方法의 信賴度 客觀度와 妥當度의 檢定에 관한 硏究

최민동 慶星大學校 1997 論文集 Vol.18 No.2

There are many methods for body fat estimation. Especially the skinfolds thickness measurement was used as an important one of many methods, because of its convenient aspect. So it was considered to be tested its test conditions again for increasing of precision in body fat estimation. Therefore this study was designed to examine reliability, objectivity, and validity of skinfolds thickness method there were vertical, horizontal, and diagonal measurement method for body composition evaluation, for this study, the subjects were selected 53 male students in 3rd-4th grade in Kyung Sung university. The results were as follows : 1. Among values of all most parts by vertical, horizontal, and diagonal method, except suprailiac and calf parts, significant differences of about 0.27-0.73mm were found. 2. The reliabilities for each part of vertical, horizontal, and diagonal method were found highly as 0.9232-0.9981. 3. The objectivities of vertical, horizontal, and diagonal method for each part were found more than about 0.92. But in face part, vertical and horizontal method with 0.7689-0.9072 were a little lower than diagonal method with 0.9095. In calf part, vertical method with 0.7721-0.7989 was lower than horizontal and diagonal methods with 0.8089-0.8588. 4. In each validity to bioelectrical impedance(BI) method, diagonal method was found r=0.7529, horizontal r=0.7418, and vertical method r=0.7391. Abdominal part with r=0.6852-0.7201 was found the highest part of all parts in each method. But face part with r=0.4790-0.5305 was the lowest of all parts. And the other parts were found about r=0.6. 5. In constitution of estimation formula, the following formulas that had abdominal part as explainable variable for body fat estimation were found as valid things. 1) vertical method : %fat=0.347X +8.911 (R=0.6952. SEE=2.2169) 2) horizontal method : %fat=0.345X +7.782 (R=0.7079. SEE=2.1779) 3) diagonal method : %fat=0.352X +8.838 (R=0.7201. SEE=2.1092) X : abdominal skinfolds thickness

맥반석의 용출시험 및 중금속 흡착특성

김종부,신명국,성낙환,최문정,김경주,이동석 강원대학교 산업기술연구소 2000 産業技術硏究 Vol.20 No.A

This experiment was done to investigate the leaching and adsorption properties of heavy metals on porphyry. The comparison with respect to the leachability of heavy metals from porphyry between the Korea Standard Leaching Test (KSLT) and the Toxicity Characteristic Leaching Procedure (TCLP) was carried out. The fractional composition of leachate and the total concentration of heavy metal of porphyry were studied through Sequential Extraction Procedure (SEP) and EPA Method 3050. Adsorption experiment of porphyry has pointed out that the optimum dosage of porphyry for 50ppm Pb was over 10g/L, the effective particle size for absorption was below 200 mesh and the optimum pH was about 7. From the Freundlich' adsorption equation, l/n was 1.0722, and k was 0.0041. After adsorption, the fractional composition of Pb was changed. The exchangeable, carbonate, reducible fractions were increased, and the organic fraction was not changed, and the residual fraction was decreased.

Recovery Voltage Measurements of Oil-immersed Transformer

Ming-hua Li,Ming Dong,Yan-ming Qu,Zhang Yan 한국전기전자재료학회 2006 Transactions on Electrical and Electronic Material Vol.7 No.5

One of the methods currently being investigated as a possible non-intrusive diagnostic tool for condition monitoring of power transformer and cable is the recovery voltage measurement, which will be improving the ability to detect the content of water concentration and the ageing process in the insulation system and may thus be an indicator of insulation quality and its ageing status. The polarization phenomenon was studied using RVM with oil-paper samples. In order to interpret its mechanism, the Extended Debye model was introduced. With different circuit parameters, various simulation results were gotten. Furthermore, with the test samples of different ageing condition, measurements are accomplished in the lab. On the basis of this experiment as well as theoretical analysis, correlations between polarizations and ageing were analyzed.

Numerical investigation on Rayleigh-Bénard-Marangoni flow of water near the maximum density in cylindrical pools with large aspect ratios

Dong-Ming Mo,Liu-Zhu Cao,Li Zhang,Shuang Ye,You-Rong Li 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.10

We report a set of direct numerical simulation results on Rayleigh-BénardMarangoni (R-B-M) flow of cold water in the cylindrical pools. The heat transfer between the free surface and the environment is considered. The aspect ratio Г of the cylindrical pool varies from 2 to 8. Rayleigh (Ra) and Biot (Bi) numbers are respectively confined in Ra ≤ 10 4 and 0 < Bi ≤ 50. The flow onset critical Ra is determined and the influences of Bi and the density inversion parameter ( mΘ ) on the critical Ra are analyzed. The primary bifurcation flow structures of R-B-M flow are shown and the evolution of the flow structures with Ra and Bi at different mΘ is observed. Furthermore, the heat transfer ability is estimated by Nusselt number. The results indicate that the critical Ra of the flow onset increases with increasing Bi and Θ m . But it decreases with the increase of Г. The primary bifurcation pattern is multicellular flow. With increasing Г, the number of flow cells in multicellular flow increases fast. With increasing Ra, the up-triangular and up-quadrilateral flow structures appear at Г = 4, and finally transits to the up one-torus. With increasing Ra and Г, and decreasing Θ m , average Nusselt number increases monotonically. However, with increasing Bi, it first increases, and then decreases.

Exploring innovation in Shanzhai: the case of mobile phones

Ming Dong,Stephen Flowers 기술경영경제학회 2016 ASIAN JOURNAL OF TECHNOLOGY INNOVATION Vol.24 No.2

Chinese innovation in low-cost, ‘good enough’ electronic products has been very active. Suchproducts may be copies of current branded goods that are positioned as straightforwardcounterfeits, knock-offs, or Shanzhai. In this paper, we argue that this simplistic analysis isinadequate to describe novel approaches to the production of new goods and that a newsystem of innovation is emerging. This novel innovation system has a distinct set of contextspecific actors and intra- and inter-firm structures that have aided the technology innovationsand the development of new business models. This paper employs a case study method anduses qualitative data to explore how innovations emerge in new mobile technologies and theinnovation system that supports their emergence. It examines the Shanzhai mobile phoneindustry as a case and provides a first attempt to map the innovation system that hasdeveloped. The results suggest that the distinctive characteristics of the Chinese innovationsystem contribute to the innovations around the product and the production processes. It further confirms the suitability of applying the innovation system approach to Shanzhaiphenomenon, and explores new forms of innovation dynamics. The paper concludes with aninitial agenda for further research in this area.

A Study on the Landscape Planning and Construction along the Yellow River in Shandong

( Dong Ming Yu ),( Yong Ji Piao ),( Zhen Wang ),( Juan Zhang ) 한국녹지환경디자인학회 2006 녹지환경학회지 Vol.2 No.1

Waterfront is the origin of human culture and the vivid district of ecology and landscape in a city. Yellow River is the mother river to Chinese people and also is one of the most complicated and difficultly managed rivers in the world. Based on the analysis of present situations of landscape planning and construction of Shandong Yellow River, some countermeasures are put forward in the paper.

Linear-stability analysis of thermocapillary convection in an annular two-layer system with free surface subjected to a radial temperature gradient

Dong-Ming Mo,Deng-Fang Ruan 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.7

The linear-stability analyses of thermocapillary flow in an annular two-layer system subjected to a radial temperature gradient were conducted. The annular pool with upper free surface and adiabatic bottom is filled with immiscible double layer liquid of 5cSt silicone oil / HT-70. The outer wall of the annular pool is heated and inner wall is cooled. The results indicate that the critical Marangoni number decreases with the increase of aspect ratio and radius ratio. The effect of the ratio of the lower liquid thickness to the total double layer thickness on the convection onset is complicated. The minimum critical Marangoni number is located near the thickness ratio of 0.125. Moreover, there is one Hopf bifurcation from the steady to oscillatory flow when the thickness ratio varies from 0.625 to 0.75. At the same time, two types of flow destabilizing mechanism are identified. Comparing with the results with upper rigid wall, it can be found that the stability of thermocapillary convection in annular two-layer system with the free surface reduces largely.

A Novel Ginsenosidase from an Aspergillus Strain Hydrolyzing 6-O-Multi- Glycosides of Protopanaxatriol-Type Ginsenosides, Named Ginsenosidase Type IV

( Dong Ming Wang ),( Hong Shan Yu ),( Jian Guo Song ),( Yu Feng Xu ),( Chun Ying Liu ),( Feng Xie Jin ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.10

Herein, a novel ginsenosidase, named ginsenosidase type IV, hydrolyzing 6-O-multi-glycosides of protopanaxatrioltype ginsenosides (PPT), such as Re, R1, Rf, and Rg2, was isolated from the Aspergillus sp. 39g strain, purified, and characterized. Ginsenosidase type IV was able to hydrolyze the 6-O-α-L-(1→2)-rhamnoside of Re and the 6-O-β-D- (1→2)-xyloside of R1 into ginsenoside Rg1. Subsequently, it could hydrolyze the 6-O-β-D-glucoside of Rg1 into F1. Similarly, it was able to hydrolyze the 6-O-α-L-(1→2)- rhamnoside of Rg2 and the 6-O-β-D-(1→2)-glucoside of Rf into Rh1, and then further hydrolyze Rh1 into its aglycone. However, ginsenosidase type IV could not hydrolyze the 3-O- or 20-O-glycosides of protopanaxadioltype ginsenosides (PPD), such as Rb1, Rb2, Rb3, Rc, and Rd. These exhibited properties are significantly different from those of glycosidases described in Enzyme Nomenclature by the NC-IUBMB. The optimal temperature and pH for ginsenosidase type IV were 40℃ and 6.0, respectively. The activity of ginsenosidase type IV was slightly improved by the Mg2+ ion, and inhibited by Cu2+ and Fe2+ ions. The molecular mass of the enzyme, based on SDS-PAGE, was noted as being approximately 56 kDa.

ORIGINAL ARTICLE : From Blemish to Decoration: the Landscape of Municipal Facilities in Urban Green Space

( Dong Ming Yu ),( Yong Ji Piao ),( Tae Dong Cho ),( Gab Soon Park ) 한국환경과학회 2014 한국환경과학회지 Vol.23 No.1

As the combination of technique and art, environmental facilities have the function to improve the image of urban green space. Among these facilities, some can be designed and arranged reasonably. But to the facilities of sewage, electricity and sanitation and so on, are usually ignored and become the blemish in the green space. Based on the analysis of the status, taking the design of well cover of sewage as an example, the landscape approaches are discussed from form, material, color and artistic technique and so on.