Microsatellite instability and its correlation with clinicopathological features in a series of thyroid tumors prevalent in iodine deficient areas

Minal Vaish,Anjali Mishra,Manish Kaushal,Saroj K Mishra,Balraj Mittal 생화학분자생물학회 2004 Experimental and molecular medicine Vol.36 No.2

Thyroid tumors display diverse spectrum of his-topathological groups with geographic variation in its prevalence. Influence of iodine deficiency (a major causative factor) in its etiology, prevalence, or aggressiveness is debatable which reflects the existence of various genetic events in pathoge-nesis. The present study was undertaken to study the role of Microsatellite instability (MSI) or LOH (loss of heterozygosity), an indicator of defective mismatch repair system as a genetic change and tumors. Tumor tisues from total thyroidectomy surgical specimens and blood (matched control) of 36 patients from iodine deficient areas (10 benign; 26 malignant) were obtained after their consent. Urinary iodine analysis was done by alkali ash method for which 10 ml of urine was colected from 18 patients before surgery. Genomic DNA, isolated from tumor tissue and blod was am-plified by polymerase chain reaction (PCR) using mono and dinucleotide markers - BAT-26, BAT-40, TGF(RII, IGFIIR, hMSH3, BAX, D2S123, D9S283, on 8% denaturing polyacrylamide gel folowed by autoradiography. Of total, 66.6% of tumors [70% (7/10) benign and 65.4% malignant cases (17/26)] showed MSI/LOH. Strong asociation of MSI/LOH with low iodine (P = 0.01) and with AMES risk groups i.e. age (P= 0.02), tumor size (P= 0.04) and metastases (P = 0.002) in thyroid tumors was observed. This may help in predicting the biolo-gical behaviour and strengthening the hypothesis thyroid tumors. Our results further substantiate the risk group clasification and help in deciding the treatment modality in particular patient.

Evaluation of antipsychotic and anti-diarrhoeal activities of ethanolic extractof roots of Rubia cordifolia Linn

Minal T Harde,Avinash S Khairnar,Ameya S Kasture,Sanjay B Kasture 경희대학교 융합한의과학연구소 2008 Oriental Pharmacy and Experimental Medicine Vol.8 No.1

The objective of the present study was to assess the antipsychotic and antidiarrhoeal activities of ethanolic extract of roots of Rubia cordifolia in mice and rats. The antipsychotic activity of ethanolic extract of roots of Rubia cordifolia (ERC) was evaluated by observing its effect on amphetamineinduced stereotyped behavior in mice. Effect of ERC was also studied on motor coordination and locomotion in mice. The antidiarrhoeal activity was evaluated using castor oil-induced diarrhoea and excretion of sodium and potassium ions in the intestinal secretion in rats and gastrointestinal transit in mice. The ERC inhibited amphetamine-induced stereotyped behaviour, diminished locomotion and impaired motor coordination. ERC inhibited castor oil-induced diarrhoea, decreased both sodium and potassium excretion in the intestine and decreased gastrointestinal transit. Thus the present study confirms the anti-diarrhoeal activity of Rubia cordifolia. Further studies are necessary to evaluate the potential of Rubia cordifolia as an antipsychotic. The objective of the present study was to assess the antipsychotic and antidiarrhoeal activities of ethanolic extract of roots of Rubia cordifolia in mice and rats. The antipsychotic activity of ethanolic extract of roots of Rubia cordifolia (ERC) was evaluated by observing its effect on amphetamineinduced stereotyped behavior in mice. Effect of ERC was also studied on motor coordination and locomotion in mice. The antidiarrhoeal activity was evaluated using castor oil-induced diarrhoea and excretion of sodium and potassium ions in the intestinal secretion in rats and gastrointestinal transit in mice. The ERC inhibited amphetamine-induced stereotyped behaviour, diminished locomotion and impaired motor coordination. ERC inhibited castor oil-induced diarrhoea, decreased both sodium and potassium excretion in the intestine and decreased gastrointestinal transit. Thus the present study confirms the anti-diarrhoeal activity of Rubia cordifolia. Further studies are necessary to evaluate the potential of Rubia cordifolia as an antipsychotic.

Evaluation of antipsychotic and anti-diarrhoeal activities of ethanolic extract of roots of Rubia cordifolia Linn

Harde, Minal T.,Khairnar, Avinash S.,Kasture, Ameya S.,Kasture, Sanjay B. Kyung Hee Oriental Medicine Research Center 2008 Oriental pharmacy and experimental medicine Vol.8 No.1

The objective of the present study was to assess the antipsychotic and antidiarrhoeal activities of ethanolic extract of roots of Rubia cordifolia in mice and rats. The antipsychotic activity of ethanolic extract of roots of Rubia cordifolia (ERC) was evaluated by observing its effect on amphetamineinduced stereotyped behavior in mice. Effect of ERC was also studied on motor coordination and locomotion in mice. The antidiarrhoeal activity was evaluated using castor oil-induced diarrhoea and excretion of sodium and potassium ions in the intestinal secretion in rats and gastrointestinal transit in mice. The ERC inhibited amphetamine-induced stereotyped behaviour, diminished locomotion and impaired motor coordination. ERC inhibited castor oil-induced diarrhoea, decreased both sodium and potassium excretion in the intestine and decreased gastrointestinal transit. Thus the present study confirms the anti-diarrhoeal activity of Rubia cordifolia. Further studies are necessary to evaluate the potential of Rubia cordifolia as an antipsychotic.

Protocol for management of pregnant patients requiring emergency minor oral surgical procedures: a prospective study in 52 patients

Ajinath Nanasaheb Jadhav(Ajinath Nanasaheb Jadhav ),Shushma G(Shushma G ),Uzma Hamidullah Siddiqui(Uzma Hamidullah Siddiqui ),Minal Sharma(Minal Sharma ),Yaseer Irfan Shaikh(Yaseer Irfan Shaikh ),Pooj 대한구강악안면외과학회 2023 대한구강악안면외과학회지 Vol.49 No.1

Objectives: Dental or maxillofacial emergencies are uncommon during pregnancy, but if they occur, they are challenging to treat due to potential risks. The mother should not be denied necessary medical or dental care because of pregnancy. The aim of the study is to observe outcomes of pregnancy in patients requiring emergency minor oral surgical procedures during gestation and to determine the safety of the pregnant woman undergoing the procedure and the fetus. Materials and Methods: The study was conducted on 52 pregnant women requiring emergency oral surgical procedures. A standard treatment protocol for treatment of specific entities was followed. Close monitoring and observation were the primary goal of treatment. All patients were followed postoperatively until complete recovery from the surgical procedures and then until birth of the baby. A control group of 52 healthy pregnant patients who did not require oral surgical procedures was considered for statistical analysis. The measurements to calculate observation were fetal loss (spontaneous abortion), preterm birth, low-birth weight, or incidence of any congenital anomalies in the baby and its association with surgical procedures. Results: No fetal loss occurred in any of the cases. However, four patients experienced preterm birth and seven neonates exhibited low birth weights. No congenital abnormalities were discovered. In one instance, a patient who underwent surgery for a mandibular symphysis fracture under general anesthesia in the 31st week of pregnancy experienced labor pain on the fourth postoperative day, requiring an emergency Caesarean section. Conclusion: The results of our study demonstrate that, compared to the control group, minor emergency surgeries performed during pregnancy have no discernible negative effects on the fetus. These procedures can safely be performed by adhering to our described protocols.

Is long term storage of cryopreserved stem cells for hematopoieticstem cell transplantation a worthwhile exercise in developingcountries?

Santhosh Kumar Devadas,Minal Khairnar,Sumathi S Hiregoudar,Shashank Ojha,Sachin Punatar,Alok gupta,Anant Gokarn,Pallavi Bhole,Sadhana Kannan,Navin Khattry 대한혈액학회 2017 Blood Research Vol.51 No.4

Background: Stem cell units (SCUs) that are cryopreserved prior to both autologous and allogeneic hematopoietic stem cell transplants (for donor lymphocyte infusion) remain unused or partially used several times, and become an increased burden to blood banks/SCU repositories. Because of the scarcity of data regarding the duration for which the storage is useful, there is no general consensus regarding disposal of SCUs. Methods: We conducted a retrospective audit of SCU utilization in 435 patients who planned to undergo either autologous stem cell transplantation (auto-SCT) (N=239) or allogeneic stem cell transplantation (allo-SCT) (N=196) at a tertiary cancer care center between November 2007 to January 2015. Results: Our cohort consisted of 1,728 SCUs stored for conducting auto-SCT and 729 SCUs stored for conducting donor lymphocyte infusions (DLIs) after allo-SCT. Stem cells were not in-fused in 12.5% of patients who had planned to undergo auto-SCT, and 80% of patients who underwent allo-SCT never received DLI. Forty-one percent of SCUs intended for use in auto-SCT remained unutilized, with a second auto-SCT being performed only in 4 patients. Ninety-four percent of SCUs intended for carrying out DLIs remained unused, with only minimal usage observed one year after undergoing allo-SCT. Conclusion: The duration of storage of unused SCUs needs to be debated upon, so that a consensus can be reached regarding the ethical disposal of SCU.

Is long term storage of cryopreserved stem cells for hematopoieticstem cell transplantation a worthwhile exercise in developingcountries?

Santhosh Kumar Devadas,Minal Khairnar,Sumathi S Hiregoudar,Shashank Ojha,Sachin Punatar,Alok gupta,Anant Gokarn,Pallavi Bhole,Sadhana Kannan,Navin Khattry 대한혈액학회 2017 Blood Research Vol.52 No.4

Background: Stem cell units (SCUs) that are cryopreserved prior to both autologous and allogeneic hematopoietic stem cell transplants (for donor lymphocyte infusion) remain unused or partially used several times, and become an increased burden to blood banks/SCU repositories. Because of the scarcity of data regarding the duration for which the storage is useful, there is no general consensus regarding disposal of SCUs. Methods: We conducted a retrospective audit of SCU utilization in 435 patients who planned to undergo either autologous stem cell transplantation (auto-SCT) (N=239) or allogeneic stem cell transplantation (allo-SCT) (N=196) at a tertiary cancer care center between November 2007 to January 2015. Results: Our cohort consisted of 1,728 SCUs stored for conducting auto-SCT and 729 SCUs stored for conducting donor lymphocyte infusions (DLIs) after allo-SCT. Stem cells were not in-fused in 12.5% of patients who had planned to undergo auto-SCT, and 80% of patients who underwent allo-SCT never received DLI. Forty-one percent of SCUs intended for use in auto-SCT remained unutilized, with a second auto-SCT being performed only in 4 patients. Ninety-four percent of SCUs intended for carrying out DLIs remained unused, with only minimal usage observed one year after undergoing allo-SCT. Conclusion: The duration of storage of unused SCUs needs to be debated upon, so that a consensus can be reached regarding the ethical disposal of SCU.

Future Perspective and Current Aspects of Internet of Things Enable Design

Evedeep Kaur Bhatia,Simran Ohri,Gurbani Kaur,Minal Dhankar,Sweety Dabas 보안공학연구지원센터 2015 International Journal of Software Engineering and Vol.9 No.8

In this work, we are going to survey the latest progress in Internet of Things (IoTs) and also design IoTs enable electronics design like frame buffer, content addressable memory, and key generator for encryption and decryption. We are analyzing future perspective, overall impact, and its role in every corner of life, characteristics features and current aspects of IoTs. Apart from this, we study how this concept came into existence and its emergence changes our lives. In this paper, we have also designed IoTs enable Frame Buffer on FPGA for Object Tracking, IoTs enable Content Addressable Memory for processor and IoTs enable Key Generator for Green Communication. In order to make IOTs enable design, we are embedding a 128-bit Internet Protocol Version 6 (IPv6) address in each and every design that enables.