가압가열 및 Microwave 처리가 생면의 품질에 미치는 영향

박시우,김꽃봉우리,김민지,강보경,박원민,김보람,박홍민,최정수,최호덕,안동현,Bark, Si-Woo,Kim, Koth-Bong-Woo-Ri,Kim, Min-Ji,Kang, Bo-Kyeong,Pak, Won-Min,Kim, Bo-Ram,Park, Hong-Min,Choi, Jung-Su,Choi, Ho-Duk,Ahn, Dong-Hyun 한국식품영양학회 2013 韓國食品營養學會誌 Vol.26 No.4

중력분을 이용하여 제면한 후 가압가열, microwave 및 가압가열과 microwave 병행 처리한 후, 생면 및 삶은 면의 품질에 미치는 영향에 대하여 알아보았다. 생면의 pH는 microwave 1 min 처리구에서 무처리구와 유의적인 차이를 보이지 않았으며, 수분 함량은 autoclave 50 min 처리구에서 무처리구와 유의적인 차이를 보이지 않았고, 그 외의 처리구에서는 모두 수분 함량이 감소함을 확인하였다. 면의 색도 측정 결과는 명도는 무처리구에 비해 모든 처리구에서 감소하였으며, 적색도는 microwave 1 min 처리구는 감소한 반면, 나머지 처리구는 증가하였다. 황색도는 autoclave 50 min 처리구와 autoclave 50 min/microwave 1 min 병행 처리구에서 증가한 반면, 나머지 물리적 처리구에서는 감소하였다. 삶은 면의 색도에서는 명도, 적색도, 황색도 모두에서 무처리구에 비하여 물리적 처리구에서 증가한 값을 나타내었다. 물성 측정 결과는 경도, 부착성, 응집성, 검성, 복원성에서 무처리구와 비교시 microwave 1 min 처리구는 유의적으로 감소하였으며, 그 외 처리구에서는 유의적으로 증가한 값을 나타내었다. 탄력성과 전단력은 모든 처리구에서 유의적으로 증가하였으며, 인장력은 가압가열 30 min 처리구와 무처리구가 유의적인 차이를 보이지 않았으나, 나머지 물리적 처리구에서는 유의적으로 증가하였다. 관능 평가 결과는 생면의 경우 색 항목에서 물리적 처리구들이 유의적으로 낮은 점수를 받았다. 맛, 질감, 향 항목에서는 무처리구와 물리적 처리구간 유의적 차이를 보이지 않았다. 전체적인 기호도에서는 microwave 1 min 처리구가 가장 높은 점수를 얻었으며, 가압가열(50 min)과 microwave(1 min) 병행 처리구는 무처리구와 비슷한 점수를 얻었다. 따라서 제면 후 가압가열 및 microwave 처리가 생면 및 삶은 면에 있어서 색과 질감 항목을 보완, 개선시킨다면 알러겐성이 저감화된 면을 제품화하는데 적합할 것으로 사료되어진다. This study was conducted to determine the effects of physical treatments for quality of wet noodles. Noodles were being tried with a microwave (for 1 min), an autoclave (for 30 or 50 min), and both autoclave and microwave (for 30/1 min or 50/1 min). The results showed that the pH levels were slightly decreased after treatments of autoclave and autoclave/microwave. The moisture contents were considerably decreased as compared to the control except autoclave (50 min). After all treatments, the lightness was decreased in all samples, but, redness was increased (except microwave) and the yellowness was increased after autoclave (50 min) and autoclave/microwave (50/1 min). Texture was increased as compared to the control except microwave. In the sensory evaluation, the noodles treated with microwave, autoclave (50 min), and autoclave/ microwave (50/1 min) showed a high score in overall preference. From these results, both the autoclave and microwave methods can be applied to the wet noodles without diminishing its quality to a great extent.

물리적 처리에 의한 강력분 밀가루 Gliadin의 항원성 변화

강보경(Bo-Kyeong Kang),김꽃봉우리(Koth-Bong-Woo-Ri Kim),김민지(Min-Ji Kim),박시우(Si-Woo Bark),박원민(Won-Min Pak),김보람(Bo-Ram Kim),안나경(Na-Kyung Ahn),최연욱(Yeon-Uk Choi),최정수(Jung-Su Choi),최호덕(Ho-Duk Choi),안동현(Dong-Hyun A 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.4

본 연구에서는 가압가열 및 microwave 처리가 gliadin의 항원성에 미치는 영향을 살펴보기 위해 강력분에 가압가열과 microwave를 단독 또는 병행으로 처리하여 Ci-ELISA, SDS-PAGE 및 immunoblotting을 실시하였다. 가압가열 처리의 경우 시간이 길어질수록 IgG와의 결합력이 감소하였으며, 특히 50분 처리구에서 약 87%로 가장 낮은 결합력을 보였다. 또한 SDS-PAGE와 immunoblotting 결과에서도 무처리구에서 강하게 보였던 gliadin band가 가압가열처리에 의해 거의 소실되고 항체와 반응하지 않았다. 가압가열 및 microwave를 병행 처리 시도 마찬가지로 gliadin의 결합력이 감소하였으며, 처리구 중 가압가열 50분, microwave 5분 처리구에서 약 93%로 가장 낮은 결합력을 보였다. 반면 microwave를 단독으로 처리한 경우에는 일부 단백질의 변화는 관찰되었으나, 항원성 감소에는 효과가 없음을 확인하여 단백질 변화가 항원성에는 큰 효과를 준 것 같지 않다. 이상의 결과를 통해 가압가열 단독 처리 및 가압가열 및 microwave의 병행처리 시 gliadin의 항원성이 감소함을 확인하였다. This study was conducted to evaluate the effects of physical treatments on the antigenicity of gliadin in strong wheat flour. Strong wheat flour was treated with an autoclave (5, 10, 30, 50 min), a microwave (1, 5, 10 min), or both (10, 30, 50 min/ 5, 10 min), followed by SDS-PAGE, immunoblotting, and Ci-ELISA using anti-gliadin IgG. The results indicated that the binding ability of IgG to gliadin in strong wheat flour slightly decreased after autoclaving or autoclaving/microwaving. In particular, the binding ability was reduced to about 87% after autoclaving for 50 min and to 89% after autoclaving/microwaving (50/5 min). In addition, gliadin bands in the 50 min autoclaved group disappeared in both SDS-PAGE and immunoblotting. On the other hand, the antigenicity of gliadin was unaffected by microwaving alone. In conclusion, the results of this study suggest that autoclaving may reduce the antigenicity of gliadin in strong wheat flour.

반응표면분석법을 이용한 배 퓌레 열수처리 조건 최적화

박민경(Min Kyeong Park),조용식(Yong Sik Cho),장현욱(Hyun Wook Jang) 한국식품영양과학회 2022 한국식품영양과학회지 Vol.51 No.11

본 연구는 열수처리 온도(50, 70, 90°C) 및 열수처리 시간(15, 30, 45분)을 달리하여 배 퓌레를 제조한 후 품질특성을 분석하여 반응표면분석법을 통해 최적의 열수처리 조건을 구하고자 하였고, 중심합성계획법에 따라 총 9가지의 실험점에서 진행하였다. 열수처리 온도 및 시간을 독립변수로 정하고, 품질특성에 관련된 적색도, ΔE값, 일반세균, 대장균군, polyphenol oxidase 활성, 총 폴리페놀 함량, 총 플라보노이드 함량을 종속변수로 설정하였다. 독립변수가 종속변수에 미치는 영향에 대해 분석한 결과, 적색도와 갈변을 나타내는 ΔE값은 각각 -0.38~1.20, 0.97~11.88의 범위로 측정되었고, 반응식의 R2은 각각 0.9754, 0.9818이었다. 일반세균 및 대장균군의 생균수는 각각 0.00~4.91 log CFU/mL, 0.00~4.03 log CFU/mL의 범위였고, 반응식의 R²은 0.9849, 0.9714였다. 갈변의 원인이 되는 polyphenol oxidase 활성은 122.57~235.13 unit 범위였으며, 반응식의 R²은 0.8616의 값을 보였다. 배 퓌레의 유효성분으로 총 폴리페놀 함량 및 총 플라보노이드 함량은 각각 19.33~24.54 GAE mg/100 g, 4.56~5.78 GAE mg/100 g 범위였으며, 이때 반응식의 R²은 각각 0.9181, 0.9097을 나타내었다. 이를 바탕으로 contour map을 superimposing 하여 최적 열수처리 조건을 설정한 결과, 최적화된 열수처리 조건의 온도범위는 69.3~80.4°C, 시간 범위는 16.8~25.8분으로 나타났으며, 최적점은 74.6°C, 20.7분으로 나타났다. 이에 대해 모델의 신뢰성을 검증하기 위해 예측값과 실험값을 비교한 결과, 각 반응변수의 실험값은 모두 예측값의 95% 신뢰구간 및 예측구간 범위에 들어와 모델의 적합성을 확인하였다. This study analyzed the quality characteristics of pear puree by varying the hydrothermal treatment temperature (50, 70, and 90°C) and time (15, 30, and 45 min) and sought to determine the optimal hydrothermal treatment conditions through the response surface methodology. A total of nine experimental points were studied according to the central composite design. The temperature and time were set as independent variables, and the redness, ΔE value, aerobic bacteria, coliform group, polyphenol oxidase (PPO) activity, total polyphenol content (TPC), and total flavonoid content (TFC) related to the quality characteristics were set as dependent variables. The redness and the ΔE value were measured as −0.38∼1.20 and 0.97∼11.88, respectively, and the R² value was 0.9754 and 0.9818, respectively. The R2 value of viable cell counts were 0.9849 and 0.9714 in the aerobic bacteria and coliform groups. The PPO activity was 122.57∼235.13 g/min, and the R² value was 0.8616. The TPC and TFC as active ingredients of pear puree were in the range of 19.33∼24.54 and 4.56∼5.78 GAE mg/100 g, respectively, and the R² values were 0.9181 and 0.9097, respectively. Based on this, the contour map was superimposed to set the optimal hydrothermal treatment conditions. The optimum points derived were 74.6°C and 20.7 min. When the predicted and the experimental values were compared to verify the reliability of the model, all the experimental values of each of the response variables were within the 95% confidence and prediction intervals, confirming the suitability of the model.

Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid and Sensitive Detection of Barley Yellow Dwarf Virus in Oat

Na-Kyeong Kim,Sang-Min Kim,Rae-Dong Jeong 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.5

Barley yellow dwarf virus (BYDV) is an economically important plant pathogen that causes stunted growth, delayed heading, leaf yellowing, and purple leaf tip, thereby reducing the yields of cereal crops worldwide. In the present study, a reverse transcription recombinase polymerase amplification (RT-RPA) assay was developed for the detection of BYDV in oat leaf samples. The RT-RPA assay involved incubation at an isothermal temperature (42°C) and could be performed rapidly in 5 min. In addition, no cross-reactivity was observed to occur with other cereal-infecting viruses, and the method was 100 times more sensitive than conventional reverse transcription polymerase chain reaction. Furthermore, the assay was validated for the detection of BYDV in both field-collected oat leaves and viruliferous aphids. Thus, the RT-RPA assay developed in the present study represents a simple, rapid, sensitive, and reliable method for detecting BYDV in oats.

A Min-Max Distance-based Preference Boundary Applied for Recommending New Items

Hyea Kyeong Kim,Min Kyu Jung,Moon Kyoung Jang,Jae Kyeong Kim 한국경영정보학회 2011 한국경영정보학회 학술대회논문집 Vol.2011 No.06

Expression mechanism of tryptophan hydroxylase 1 in mouse islets during pregnancy.

Iida, Hitoshi,Ogihara, Takeshi,Min, Mun-kyeong,Hara, Akemi,Kim, Yeong Gi,Fujimaki, Kyoko,Tamaki, Motoyuki,Fujitani, Yoshio,Kim, Hail,Watada, Hirotaka Journal of Endocrinology (Ltd. by Guarantee) 2015 Journal of molecular endocrinology Vol.55 No.1

<P>Serotonin signaling plays key roles in augmentation of pancreatic β-cell function during pregnancy. Increased expression of tryptophan hydroxylase 1 (Tph1), a rate-limiting enzyme for serotonin synthesis by lactogenic hormones, is involved in this phenomenon. To investigate its mechanisms, we here performed 5'-RACE and identified β-cell-specific transcription initiation sites for Tph1. Prolactin enhanced the expression of mRNA containing these exons; however, reporter gene plasmids containing the proximal 5'-flanking region of these exons did not show prolactin responsiveness in MIN6 cells. Prolactin-induced Tph1 expression was inhibited by a Jak2 inhibitor and was partially inhibited by an MEK1/2 or PI3K inhibitor. Therefore, we analyzed interferon γ-activated sequences (GAS) and found GAS-A about 9-kbp upstream of the transcription start site. The reporter gene plasmid containing the GAS-A region linked to a heterologous promoter showed increased promoter activity by prolactin, which was inhibited by the forced expression of a dominant-negative mutant form of Stat5A and a Jak2 inhibitor. Chromatin immunoprecipitation analysis showed that prolactin treatment augmented Stat5 binding to the GAS-A region in MIN6 cells, as well as in isolated mouse islets, and that Stat5 recognized the GAS-A region in pregnant mouse islets. In addition, the transactivation activity of Stat5 was enhanced by prolactin through the Erk and PI3K pathways in MIN6 cells. Finally, serotonin expression was attenuated in islets of β-cell-specific Stat5-deficient mice compared with that of control littermates during pregnancy. Our findings suggest that prolactin-induced Tph1 expression is mediated by the activation of Jak2/Stat5, Erk, and PI3K pathways in β cells.</P>

Development of in-situ electro-generated chlorine leaching and its application to the leaching of platinum

Kim Min-Seuk,Kim Rina,Chung Kyeong-Woo,Lee Jae-Chun 한국자원공학회 2022 Geosystem engineering Vol.25 No.4

An in-situ electro-generated chlorine leaching with a closed recycling unit of unconsumed gas was developed for precious metal dissolution and applied to the leaching of platinum from a spent automotive catalyst containing 0.13 wt% Pt. Cathodic current in a cathodic compartment of the chlorine generation cell reduces the unconsumed chlorine gas into chloride ions for further chlorine generation. The electro-reduction efficiency was 99.4% in alkaline 2 M NaCl solution, whereas 80.8% in 35% HCl solution at 0.62 mmol min−1 supply rate of unconsumed chlorine gas. The 98.3% platinum from the spent automotive catalyst leached without discharging the unconsumed chlorine gas into the air at 100 g L−1 solid/liquid ratio and 80°C in a 7 M HCl solution. Increasing the solid/liquid ratio to 400 g L−1 drastically decreased the leaching percentage to 16.5% in 90 minutes by possible adsorption of leached Pt to the porous surface of the automotive catalyst carrier.

Phenyl 2‐pyridyl ketoxime induces cellular senescence‐like alterations via nitric oxide production in human diploid fibroblasts

Yang, Kyeong Eun,Jang, Hyun‐,Jin,Hwang, In‐,Hu,Chung, Young‐,Ho,Choi, Jong‐,Soon,Lee, Tae‐,Hoon,Chung, Yun‐,Jo,Lee, Min‐,Seung,Lee, Mi Young,Yeo, Eui‐,J BLACKWELL PUBLISHING 2016 AGING CELL Vol.15 No.2

<P><B>Summary</B></P><P>Phenyl‐2‐pyridyl ketoxime (PPKO) was found to be one of the small molecules enriched in the extracellular matrix of near‐senescent human diploid fibroblasts (HDFs). Treatment of young HDFs with PPKO reduced the viability of young HDFs in a dose‐ and time‐dependent manner and resulted in senescence‐associated β‐galactosidase (SA‐β‐gal) staining and G2/M cell cycle arrest. In addition, the levels of some senescence‐associated proteins, such as phosphorylated ERK1/2, caveolin‐1, p53, p16<SUP>ink4a</SUP>, and p21<SUP>waf1</SUP>, were elevated in PPKO‐treated cells. To monitor the effect of PPKO on cell stress responses, reactive oxygen species (ROS) production was examined by flow cytometry. After PPKO treatment, ROS levels transiently increased at 30 min but then returned to baseline at 60 min. The levels of some antioxidant enzymes, such as catalase, peroxiredoxin II and glutathione peroxidase I, were transiently induced by PPKO treatment. SOD II levels increased gradually, whereas the SOD I and III levels were biphasic during the experimental periods after PPKO treatment. Cellular senescence induced by PPKO was suppressed by chemical antioxidants, such as N‐acetylcysteine, 2,2,6,6‐tetramethylpiperidinyloxy, and L‐buthionine‐(<I>S</I>,<I>R</I>)‐sulfoximine. Furthermore, PPKO increased nitric oxide (NO) production via inducible NO synthase (iNOS) in HDFs. In the presence of NOS inhibitors, such as L‐NG‐nitroarginine methyl ester and L‐NG‐monomethylarginine, PPKO‐induced transient NO production and SA‐β‐gal staining were abrogated. Taken together, these results suggest that PPKO induces cellular senescence in association with transient ROS and NO production and the subsequent induction of senescence‐associated proteins<B>.</B></P>

Rapid and Visual Detection of Barley Yellow Dwarf Virus by Reverse Transcription Recombinase Polymerase Amplification with Lateral Flow Strips

김나경(Na-Kyeong Kim),이효정(Hyo-Jeong Lee),김상민(Sang-Min Kim),정래동(Rae-Dong Jeong) 한국식물병리학회 2022 Plant Pathology Journal Vol.38 No.2

Barley yellow dwarf virus (BYDV) has been a major viral pathogen causing significant losses of cereal crops including oats worldwide. It spreads naturally through aphids, and a rapid, specific, and reliable diagnostic method is imperative for disease monitoring and management. Here, we established a rapid and reliable method for isothermal reverse transcription recombinase polymerase amplification (RT-RPA) combined with a lateral flow strips (LFS) assay for the detection of BYDV-infected oat samples based on the conserved sequences of the BYDV coat protein gene. Specific primers and a probe for RT-RPA reacted and optimally incubated at 42oC for 10 min, and the end-labeled amplification products were visualized on LFS within 10 min. The RT-RPA-LFS assay showed no cross-reactivity with other major cereal viruses, including barley mild mosaic virus, barley yellow mosaic virus, and rice black streaked dwarf virus, indicating high specificity of the assay. The sensitivity of the RT-RPA-LFS assay was similar to that of reverse transcription polymerase chain reaction, and it was successfully validated to detect BYDV in oat samples from six different regions and in individual aphids. These results confirm the outstanding potential of the RT-RPA-LFS assay for rapid detection of BYDV.

검사노드 갱신 과정에서 발생하는 중복 연산을 제거한 수정된 Extended Min-Sum LDPC 복호기

김민호(Kim Min-Ho),박경빈(Park Kyeong Bin),정기석(Chung Ki-Seok) 한국통신학회 2020 한국통신학회 학술대회논문집 Vol.2020 No.2