Reduction of Bacillus cereus Contamination in Biofilms on Stainless Steel Surfaces by Application of Sanitizers and Commercial Detergent

Lee, Min-Jeong,Ha, Ji-Hyoung,Kim, Yong-Su,Ryu, Jee-Hoon,Ha, Sang-Do The Korean Society for Applied Biological Chemistr 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.1

We established the effectiveness of a washing, detergent and sanitizer treatments for reducing Bacillus cereus (B. cereus) contamination in biofilms on stainless steel surfaces. A water treatment did not affect biofilms and chlorine treatments at 200 ppm for 10 min and 300 ppm for 5 min, iodophor at 200 ppm for 10 min, and QAC at 400 ppm for 10 min were used to eliminate all B. cereus cells. B. cereus cells after detergent treatment were eliminated by chlorine at 100 ppm for 10 min, 200 ppm for 5 min, and 300 ppm for 3 min, and with iodophor at 100 ppm for 10 min and 200 ppm for 10 min, and QAC at 400 ppm for 10 min.

붕장어 부산물로 제조한 붕장어탕의 식품학적 특성

허민수(Min Soo Heu),이택상(Take Sang Lee),김혜숙(Hye-Suk Kim),지성준(Seung Joon Jee),이재형(Jae Hyoung Lee),김형준(Hyung Jun Kim),윤민석(Min Seok Yoon),박신호(Shin Ho Park),김진수(Jin-Soo Kim) 한국식품영양과학회 2008 한국식품영양과학회지 Vol.37 No.4

붕장어 가공부산물인 머리와 frame를 효율적으로 이용하기 위한 일련의 연구로 붕장어 부산물을 이용한 붕장어탕의 개발을 시도하였고, 아울러 이의 식품성분 특성에 대하여도 살펴보았다. 살균조건 및 가온일수에 따른 붕장어탕의 저장성 부여를 위한 최적 살균조건은 F? value=8분으로 판단되었다. 붕장어탕의 일반성분은 수분의 경우 90.7%, 조단백질의 경우 4.8%, 조지방의 경우 2.6%, 조회분의 경우 1.5%를 나타내었다. 붕장어탕의 엑스분 질소 함량은 243.1 ㎎/100 g으로 시판 추어탕의 208.0 ㎎/100 g보다 높았다. 붕장어탕의 총 아미노산 함량은 4,310 ㎎/100 g이었고, 주요 구성아미노산은 glutamic acid(637.3 ㎎/100 g, 14.8%), glycine(409.1 ㎎/100 g, 9.5%) 및 alanine(404.4 ㎎/100 g, 9.3%)등이었다. 붕장어탕은 관능적인 비린내는 거의 감지되지 않았고, 맛은 우수하였으며, 항산화능이 인정되었으나 ACE 저해능은 크게 기대할 수 없었다. For the effective use of the conger eel by-products, such as head and frame, Tang, which is the Korean-type soup, from conger eel by-products (TCEB) was developed and its food component characterization was compared with that of commercial Chueotang, loach Tang. According to the results of viable cells and coliform group of TCEB heated at 115oC for various times, the reasonable F0 value was 8 min. The proximate composition of TCEB was 90.7% for the moisture, 4.8% for the protein, 2.6% for the lipid, and 1.5% for the ash. The extractive-nitrogen content of TCEB was 243.1 ㎎/100 g, which was higher than that of commercial Chueotang, 208.0 ㎎/100 g. The total amino acid content of TCEB was 4,310 ㎎/100 g and its major amino acids were glutamic acid (637.3 ㎎/100 g, 14.8%), glycine (409.1 ㎎/100 g, 9.5%) and alanine (404.4 ㎎/100 g, 9.3%). TCEB was not felt in the sensual fish odor and its sensual taste was good. The health functional properties for health of TCEB were 1.29 as a PF (protection factor) for antioxidative activity and 39.4% for angiotensin Ⅰ converting enzyme (ACE) inhibiting activity.

Forced suction thrombectomy in patients with acute ischemic stroke using the SOFIA Plus device

Roh Hyun Ki,Ju Min-Wook,Byoun Hyoung Soo,Park Bumsoo,Park Kwang Hyon,Jeongwook Lim 대한뇌혈관외과학회 2022 Journal of Cerebrovascular and Endovascular Neuros Vol.24 No.3

Objective: Stent retrieval thrombectomy has recently been the standard treatment for acute ischemic stroke with large artery occlusion. However, the development of catheters for suction thrombectomy has recently led to results comparable to that of stent retrieval thrombectomy (SRT). This study aimed to analyze the safety and efficacy of forced suction thrombectomy (FST) using the SOFIA Plus (MicroVention Terumo, Tustin, CA, USA) device.Methods: We included patients with acute ischemic stroke who underwent FST using the SOFIA Plus device at our institution. Medical records and angiographic data were reviewed, and the results of this study were compared with those of other FST studies.Results: A total of 35 patients were included in this study. The occlusion sites were the internal carotid artery terminal (4), M1 segment (20), and posterior circulation (11). Of the 35 patients, FST was performed in only 21 (60%) patients, and the remaining 14 (40%) patients underwent SRT and FST. In all cases, the recanalization rate was 100%, and the average time from groin puncture to recanalization was 21±4.94 min. In particular, the average time required to reach the SOFIA Plus lesions from the groin puncture was 10.44±5.06 min and about 67% of the FST patients were recanalized at the first attempt. Three-months modified Rankin Scale (mRS) score of ≤2 was observed in 52% of the patients.Conclusions: Forced suction thrombectomy using the SOFIA Plus yielded a high recanalization rate within a shorter time. In particular, the recanalization rate was higher than that reported in previous studies using other types of suction devices.

Energy storage capabilities of nitrogen-enriched pyropolymer nanoparticles fabricated through rapid pyrolysis

Yun, Young Soo,Kim, Yu Hyun,Song, Min Yeong,Kim, Na Rae,Ku, Kyojin,An, Ji Su,Kang, Kisuk,Choi, Hyoung Jin,Jin, Hyoung-Joon Elsevier 2016 Journal of Power Sources Vol.331 No.-

<P><B>Abstract</B></P> <P>Nanostructured pyropolymers contain significant amounts of redox-active heteroatoms, have high specific surface areas, and a defective carbon microstructure, indicating good potential for pseudocapacitive charge storage. In this study, nitrogen-enriched pyropolymer nanoparticles (N-PNs-50) are fabricated from polyaniline nanotubes through rapid pyrolysis at 50 °C min<SUP>−1</SUP>. N-PNs-50 exhibit a nitrogen content of 9.8 wt%, a high specific surface area of 875.8 m<SUP>2</SUP> g<SUP>−1</SUP>, and an amorphous carbon structure with an I<SUB> <I>D</I> </SUB>/I<SUB> <I>G</I> </SUB> intensity ratio of 0.95. These unique characteristics lead to good electrochemical performances, in which reversible capacities of 660 and 255 mAh g<SUP>−1</SUP> are achieved for Li-ion and Na-ion storage, respectively, with favorable voltage characteristics (<1.5 V for Li-ions and <1.2 V for Na-ions). This study provides a more feasible production method for nitrogen-doped pyropolymers and their practicable electrochemical performances for use as an anode in energy storage devices.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Nanostructured pyropolymers (N-PNs-50) were fabricated by rapid pyrolysis. </LI> <LI> N-PNs-50 exhibited a nitrogen content of 9.8 wt% and amorphous carbon structure. </LI> <LI> N-PNs-50 exhibited a high specific surface area of 875.8 m<SUP>2</SUP> g<SUP>−1</SUP>. </LI> <LI> 660 and 255 mAh g<SUP>−1</SUP> were achieved for Li-ion and Na-ion storage, respectively. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Nitrogen-enriched pyropolymer nanoparticles (N-PNs-50) were fabricated from polyaniline nanotubes through rapid pyrolysis at 50 °C min<SUP>−1</SUP>, showing superior electrochemical performances.</P> <P>[DISPLAY OMISSION]</P>

P278 : Nonablative fractional laser as a tool to facilitate skin penetration of 5-aminolevulinic acid with minimal skin disruption

( In Jung Kang ),( Jeong Hwee Choi ),( Min Jae Gwak ),( Hee Kyeong Lim ),( Mu Hyoung Lee ),( Min Kyung Shin ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2

Background: Effective penetration of photosensitizer is an essential step in photodynamic therapy (PDT). There have been trials of several methods, including laser treatment, to facilitate prompt and sufficiently deep transdermal drug delivery. Objectives: This study was performed to evaluate the effects of nonablative fractional laser pretreatment on aminolevulinic acid (ALA) penetration of the skin. Methods: Twelve identical treatment areas of 1 × 1 cm2 on the backs of 10 healthy male subjects were mapped. Each area received laser treatment with a different energy and incubation with ALA for different times. Laser treatment was performed with a 1550 nm fractional erbium glass laser, and the laser energy was set to 20 and 50 mJ with a spot density of 50/cm2. ALA incubation time was set to 30, 60, or 180 min. Porphyrin fluorescence was measured. Results: Nonablative fractional laser-pretreated areas showed significantly increased porphyrin fluorescence compared to non-pretreated areas. Laser energy strength and ALA incubation time were positively correlated with ALA absorption. Conclusion: Nonablative fractional laser treatment effectively enhanced ALA skin penetration. Pretreatment with a nonablative fractional laser can be used for ALA-PDT to achieve higher therapeutic efficacy and shortened ALA incubation times with minimal skin barrier disruption compared to ablative laser.

단신 : 고에너지밀도 펠릿제조를 위한 목재칩 반탄화 특성

이재원 ( Jae Won Lee ),김영훈 ( Young Hun Kim ),이수민 ( Soo Min Lee ),이형우 ( Hyoung Woo Lee ) 한국화학공학회 2012 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.50 No.2

본 연구에서는 소나무 혼합수종을 이용하여 에너지 밀도 증가, 균일한 품질의 바이오매스 제공을 위해 무산소 조건에서 반탄화를 실시하였다. 반응온도는 240, 260, 280˚C로 하여 30분 동안 반응시킨 후반탄화 바이오매스 특성을 조사하였다. 침엽수혼합수종의 반탄화는 무처리 바이오매스와 비교하여 발열량이 향상되었음을 확인하였다. 반탄화 온도가 증가할수록 반탄화된 바이오매스의 탄소함량은 최대 46.55%에서 55.73%로 증가하였다. 반면 수소와 산소의 함 량은 각각 6.00%에서 5.87%, 30.55%에서 27.21%로 감소하였다. 반탄화 과정에서 주로 헤미셀룰로오스와 휘발성 물 질이 제거되었다. 280˚C에서 30분 동안 반응하였을 때 최대 발열량 5,132 kcal/kg을 나타냈다. 이것은 처리전 바이오 매스의 발열량 보다 약 13% 증가하였음을 나타내고 있다. 중량감소율과 에너지수율을 고려하여 비교한 결과 240˚C 에서 30분 동안 처리하였을 때 효과적인 반탄화가 이루어졌다. In this study, torrefaction of mixed softwood chips under anoxic condition was performed to improve energy density and maintain consistent quality of biomass. Characteristics of torrefied biomass depending on reaction time (30 min) and temperature (240, 260, 280˚C) were investigated. Torrefaction of mixed softwood chips significantly improved the heating value compared to that of untreated biomass. As the torrefaction temperature was increased, the carbon content of torrefied biomass increased from 46.55 to 55.73%, while its hydrogen and oxygen contents decreased from 6.00 to 5.87% and from 30.55 to 27.21%, respectively. Most of hemicelluloses and volatile compounds were removed during torrefaction. The highest heating value was 5132 kcal/kg when torrefaction was performed at 280˚C for 30 min. It implied that the heating value increased by 13% compared to that of original biomass. However, the condition of effective torrefaction was at 240˚C for 30 min when weight loss and energy yield was considered.

저산소성 허혈성 뇌손상을 유발시킨 어린 백서에서 Myeloperoxidase 측정 검사

양혜정(Hae Joung Yang),피대훈(Dae Hun Pee),임지혜(Ji Hye Lim),최병민(Byung Min Choi),이기형(Kee Hyoung Lee),은백린(Baik-Lin Eun) 대한소아신경학회 2002 대한소아신경학회지 Vol.10 No.2

목 적 : 예전에는 뇌경색 부위에서 발견되는 백혈구를 단지 뇌손상에 뒤따르는 생리적인 반응으로만 생각하였으나 최근 10년 사이 재관류 손상에서도 백혈구에 의한 염증 작용이 중요한 역할을 한다고 알려지면서 이들의 역할에 대한 연구가 새롭게 이루어지고 있다. PML의 존재는 세포질내 과립에서 분비되는 MPO를 생화학적으로 측정함으로서 증명할 수 있는데 저자들은 미성숙 뇌에서 저산소성 허혈증에 의한 뇌손상이 발생할 때 백혈구가 침윤되는 과정을 MPO 측정 검사를 통하여 관찰하였고, P와 L-selectin 억제제인 Fucoidin을 투여한 후 MPO의 변화를 확인하였다. 방 법 : 생후 7일된 어린 흰쥐를 사용하여 우측총 경동맥을 전기 응고시켜 자르고 8% 산소에 노출시켰다. 저산소 노출 후 회복 시간에 따라 동물을 희생시키고 뇌를 추출하여 뇌조직 1 g당 10mL의 20 mM potassium Phosphate buffer(pH 7.4)를 첨가하여 50초간 분쇄하였다. 각각의 균질회된 조직 샘플들은 4℃에서 20분 동안 원심 분리하여 상층액을 제거한 후, 침전물은 처음 조직량에 따라 조직 1 g당 10 mL의 0.5% cetylditrimethylethyl ammonium bromide(wt/vol)가 첨가된 50 mM potassium phosphate buffer(pH 6.0)를 첨가하였다. Vortex를 이용하여 buffer와 침전물을 잘 섞고 60℃수조에서 120분 동안 방치하였다. 그 후 각 조직 샘플들을 4 watts에서 음파처리하고 4℃에서 15분 동안 원심 분리를 시행하여 상층액을 새로운 tube에 옮겼다. MPO 측정검사를 위한 MPO reaction buffer는 50 mM potassium phosphate buffer(pH 6.0) 100 mL에 0.53 mM odianisidine edihydrochlorde와 0.0005% HO를 섞어 만들었다. MPO reaction solution 2.9 mL에 각각의 MPO sample 0.1 mL을 첨가하여 460 nm의 파장에서 5분 동안 MPO에 다른 흡광도 차이를 관찰하였다. 결 과 : 조직내 백혈구 침윤의 지표로 측정한 MPO 활성도는 대조군에서는 미미하였으나 총 경동백을 절단한 우측 대뇌군에서는 허혈 및 저산소증 유발 후 8시간째부터 현저히 증가되기 시작하여 24시간 경과 후 발현이 가장 높았으며, Fucoidin 50 mg/kg으로 전처치한 약물 실험군에서는 MPO 활성도가 현저히 감소하였다. 결 론 : 백혈구는 미성숙 뇌의 저산소성 허혈증에 의한 뇌손상에서 중요한 매개체 역할을 하며, 백혈구의 생화학적 활성도를 나타내는 MPO 활성도는 저산소성 허혈성 뇌손상의 정도를 측정하는 지표로 이용될 수 있다고 생각된다. Purpose : Neutrophils found around an infarcted area in the brain was once considered as only the physiologic response following the brain injury, but recent studies have shown that inflammatory responses by neutrophils play an important role in the reperfusion injury. The presence of ploymorphonuclear leukocytes(PML) is proven by biochemical assay of myeloperoxidase(MPO) Secreted in the cytoplasmic granules. We observed the process of PML infiltration on hypoxic-ischemic brain injury of immature rats by the assay of MPO activity and changes of the MPO activity after the administration of fucoidin, inhibitor of P- and L-selectin. Methods : We used a well characterized model of the brains of 7 day-old-rats, which had unilateral hypoxic and ischemic injuries(HI). Those injuries were induced by unilateral carotid artery ligation followed by timed exposure to hypoxic inspiratory gas mixture(8% O). MPO activity was measured in the brain tissue homogenates of HI rats(n=18) at 0, 2, 8, 24 and 48 hrs and in rats that received fucoidin immediately before and again after hypoxia(50 mg/kg, n=6) at 8 and 24 hrs. Controls(n=2) were rats with neither hypoxia nor ischemia. The brain samples were homogenized in 20 mM potassium phosphate buffer(pH 7.4) for 50 secs. The homogenate was centrifuged at 14,000 g at 4℃ for 15 mins and the supernatant was discarded. The tissue was pulverized, weighed, and suspended in 1 mL of 50 mM potassium phosphate buffer solution(pH 6.0) contatining 0.5% cetylditrimethylammonium bromide(wt/vol). The tissue was sonicated and centrifuged at 10,000 g for 15 mins. 200 µL of the supernatant was mixed with 1 mL of 50 mM potassium phosphate buffer solution(pH 6.0) containing 10 μL of 1.325 mM o-dianisidine hydrochloride and 170 µL of 3% hydrogen peroxide(vol/vol). Changes in absorbance at 460 nm were measured for 5 mins by using microplate reader. One unit of MPO activity was defined as that degrading 1 µmol peroxide/min at 25℃, and the result was expressed as units of MPO/100 gm tissue. Results : In HI rats, MPO activity increased at 2 hrs after HI and peaked at 24 hrs in the right hemisphere. In rats with fucoidin treatment immediately before and again after hypoxia, the MPO activity significantly decreased in both hemispheres compared with HI rats(P<0.05). MPO activity in the tissue of control rats was insignificant. Conclusion : The dynamic changes of the MPO activity suggest the important role of PMN on hypoxic-ischemic brain injuries in immature rats. MPO activity could be used as an index of the severity of injuries of hypoxic-ischemic brains.

단주기 구조물의 비탄성 응답제어를 위한 최대응답 계수를 사용한 개선된 마찰감쇠기 설계방법

민경원,이명규,이상현,김형섭 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)

The purpose of this paper is to present a design method for friction damper (FD) for inelastic response control of short period structure. A critical design parameter of FD is maximum friction force (MFF) and previous study evaluated MFF using equivalent damping ratio which is based on the maximum displacement. This procedure, however, gives the overestimated MMF for short period structure. In this study, MFF of FD is evaluated based on RMS displacement response which is obtained by using given maximum response and peak factor. Numerical analysis shows that proposed method provide a reasonable MFF of FD for short period structure.

배자기 중 인간 사지의 형태발생에 관한 연구

박형우,민양기,심용운 대한해부학회 1992 Anatomy & Cell Biology Vol.25 No.1

플립플롭의 초기화 가능성을 고려한 디지털 회로에 대한 고장 검출율의 평가 기법

閔炯福,李宰勳,金信澤 성균관대학교 1998 학술회의지원논문목록집 Vol.1998 No.-

디지탈 회로의 검사 신호에 대한 고장 검출율을 정확하게 계산하기 위해 결함 시뮬레이터를 사용한다. 그러나 결함 시뮬레이터의 실행 시간은 회로를 구성하는 게이트 수의 제곱에 비례하여 오늘날의 대규모 회로에서는 많은 CPU 시간을 소모한다. 따라서 정확도는 떨어지지만 비교적 짧은 시간 안에 회로의 검사성을 평가하기 위한 검사성 분석 알고리즘이 제안되었다. COP는 빠르고 정확하느 순차회로에 적용할 수 없으며 STAFAN은 순차회로에 적용이 가능하지만 정상 회로 시뮬레이션의 사용으로 실행시간이 많이 소모된다. 본 저자들은 정확한 고장 검출율을 단시간에 얻기 위해서 EXTASEC을 제안한 바 있다. EXTASEC은 극히 일부 회로에서 정확도가 떨어지는 현상이 발생하였으며, 이 논문에서는 EXTASEC의 문제가 플립플롭의 초기화와 관련이 있는 것을 밝히고, 개선된 알고리즘 ITEM을 제안한다. ITEM은 후진선의 반복적 계산과 초기화되지 않는 플립플롭에 대한 분석으로 고장 검출율을 정확하고 빠르게 얻을 수가 있었다. Fault simulator has been used 10 compute exact Fault coverages of test vectors lor digital circuit. But it is time consuming because execution time is proportional to square of circuit size. Recently, several algorithms for testability analysis have been published to cope with these problems. COP iS very fast and accurate but cannot be used for sequential circuits, while STAFAN can be used for sequential circuits but nerds vast amount of execution time due to good circuit simulation. We proposed EXTASEC which gave fast and accurate fault coverage. But it shows noticeable errors fora few sequential circuits. In this paper, it 1s shown that the inaccuracy is due to uninitializable flipflops, and we propose ITEM to improve the EXTASEC algorithm. ITEM iS an improved evaluation method of fault coverage by analysis of backward lines and uninitializable flipflops. It is expected to perform efficiently for very large circuits where execution time is critical.