Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

일본 오사카시에 거주하는 일부 초등학생의 체중에 대한 인식 조사

김미옥 대구보건대학 2009 대구보건대학 論文集 Vol.29 No.-

응집과 흡착에 의한 음용수중 유해 중금속의 제거

옥치상,김정아,박미연 高神大學校 保健科學硏究所 1992 보건과학연구소보 Vol.2 No.-

Water added with humic acid(10㎎/L) and heavy metals(??, ??, ??, ??, ??) were investigated the advanced removal effects of heavy metals in drinking water by alum, ion exchange resin and activated carbon. Based on the results, the removal rates of heavy metals in optimum alum coagulation (5mg/L) were ??, 38.7%; ??, 35.5%; ??, 34.8%; ??, 19.7%; ??, 4.3%. And the removal effect series of heavy metals were ??, ?? for contact time 1hr in batch with ion exchange and ?? for filtration time 1hr in column packed with ion exchange, and ?? for contact time 1hr in batch with activated carbon and ??, ?? for filtration time 1hr in column packed with activated carbon. The removal of heavy metals by continuous treatment by column packed with ion exchange and activated carbon after alum coagulation were more increase than those of each treatment, was a marked increase as contact time was increased.

진주검무의 현상학적 해석과 교육적 가치

황미숙,권오륜,차옥수 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.2

This study aims to find out aesthetic points of Jinju Gummu, a traditional Korean sword dance, in terms of beauty of phenomenal dancing arts. Physique, phenomenal body, is a physical entity and manifestation of existence. Accordingly, all kinds of movements deliver consciousness and at the same time they have a meaning of motion itself. Dance as a phenomenon embodies spirit in phenomenon itself, and further, pushes itself toward dynamic movements of body, according to features and perception of bodily experiences. Jinju Gummu imbodies thoughts, emotions, and beauty in a united universe of time and space through body movements. f regular school curriculum so that our students can learn to appreciate its aesthetic value. The dance can then become part of popular culture. With free expressions of body, dance develops personal characteristics and introduces extensive values of art, leading human beings to the world of imaginary experience and understanding and to view the phenomenon from an internal aspect of individuality that is perceptible and an external aspect of concrete ideas. This implies neither body nor movements exist without a physical shape. To perceive and understand dance is to deem both stillness of dancing body and dynamic body as time and spacious configuration. The fundamental principle of the movements in the dance tries not only to express the instinctive energy of human body, but also to make an embodiment of the integral part of human mind. In other words, Jinju Gummu can be seen as a harmonious form of samatological rythm of human body and the inner world of human mind. It is hoped that the Gummu is incorporated as a part of regular school curriculum so that our students can learn to appreciate its aesthetic value. The dance can then become part of popular culture.

성인 대학생들의 대인관계 형성에서 mentoring의 활용성 탐색 연구

장미옥 동명정보대학교 2002 저널 언론정보 Vol.1 No.-

중금속 이온 존재 하에서의 아스코르브산 자동산화반응에 대한 단백질의 영향

김미옥,유리나 울산대학교 2002 생활과학논문집 Vol.4 No.1

Effects of superoxide dismutase(SOD), catalase, and such other proteins as bovine serum albumin(BSA), ovalbumin, lysozyme, and γ-globulin on the autoxidation rates of L-ascorbic acid(AsA) in the presence of heavy metal ions in aqueous solution and buffer solution were studied. AsA was dissolved in a ultra-refined water and 0.07 M potassium phosphate buffer (pH 7.4, treated Chelex-100) at a concentration of 50μM and 5 μM Fe(Ⅲ) or 0.1 μM Cu(Ⅱ) was added, and a oxygen gas was bubbled through the solution at a flow rate of 200 ml/min at 35℃. The amount of remaining AsA in the reaction mixture was determined by using a UV spectrophotometer(at 265 nm). It was confirmed that the ratio of remaining AsA was significantly larger in the presence of SOD, catalase, BSA, ovalbumin, lysozyme, and γ-globulin than in the absence of proteins. It was suggested that the non-enzymatic effects of SOD, catalase and some other proteins might be involves in the stabilization of AsA.

저체중 또는 과체중 청소년에서 캠프 프로그램을 이용한 영양 교육효과에 관한 연구

성미경,승정자,류화춘,박재년,박동연,최미경,조경옥,최선혜,이윤신,김유경,이은주 대한지역사회영양학회 2003 대한지역사회영양학회지 Vol.8 No.4

This study was performed to evaluate the effectiveness of a nutrition education program developed for nutritionally imbalanced adolescents. A summer nutrition camp was held for 23 overweight and 16 underweight subjects. Its effectiveness with regard to was evaluated at the end of the camp and 6 months later. Nutrition knowledge, nutritional attitude, food behavior, nutrient intake, exercise habit and ideal body figures desired by the subjects. The results showed significantly higher nutrition knowledge scores at the end of the camp as compared to those obtained prior to the camp program, and these scores were maintained for at least six months. Nutrition attitude scores also improved after the education program, and these improved scores also lasted for 6 months. However, the food behavior scores measured 6 months after the education program were not significantly different from those obtained prior to the camp. Also, the exercise habit, the ideal body figures and the body figures desired by the subjects remained unchanged. When nutrient intakes of subjects were assessed before the program and 6 months later, the mean daily vitamin C intake was significantly increased after the education program. Also, the intake of iron from plant food sources increased in the overweight subjects, while less iron from animal source were consumed by the underweight subjects. Both groups tended to consume more vegetables and fruits 6 months after the education program which may have contribute to the higher vitamin C and plant-based iron intakes. These results indicate that a 4-day nutrition education camp program sustained changes in nutrition knowledge and nutrition attitude for 6 months. The increased intake of vegetables and fruits was also achieved through this education program. However, changes in dietary behavior in adolescents may require repeated education.

쇼핑성향에 따른 니트웨어 구매행동에 관한 연구

이옥희,김경희,최미현 服飾文化學會 2002 服飾文化硏究 Vol.10 No.4

The Main objective of this study was to investigate the relationship between shopping orientations and Knit wear Buying Behavior of college female students. A questionnaire was developed to measure knit wear purchasing motives, fashion information sources of knit wear, evaluation criteria of knit wear product, and general clothing buying behavior. The questionnaire was administered to 505 college female students in Chonbuk and Connnam. the data was analyzed using percentage, frequency, mean, factor analysis, Cluster Analysis and ANOVA, Duncan Multiple Range test. The results of the study were as follows: 1. The college female students were classified into fifth subdivisions by the cluster analysis; convenient shopping group, recreational shopping group, self-confident shopping group, those of Fashion-pursuit group, economic shopping group. 2. The knit wear purchasing motives of consumers were significantly different according to shopping orientation subdivision in social, personal, rational. 3. In the case of fashion information sources of knit wear, significant differences were found according to shopping orientation subdivision in mass media information, information by marketer, mail order advertisements, information by consumer. 4. The evaluation criteria of knit wear product of consumers were significantly different depending on shopping orientation subdivision in esthetic, practicality, individual expression, external criterion. 5. In the case of dissatisfactory factors for wearing knit wear product, significant differences were found according to shopping orientation subdivision. 6. In the case of asking factors to the manufactures of knit wear product, significant differences were found according to shopping orientation subdivision.

임신성 융모 종양의 Immunoperoxidase 방법에 의한 HCG, HPL 및 SP-1의 분포에 관한 면역조직화학적 연구

정미경,한운섭,김옥경 梨花女子大學校 醫科大學 醫科學硏究所 1987 EMJ (Ewha medical journal) Vol.10 No.3

Fourty-six cases of gestational trophoblastic neoplasms (32 cases of hydatidiform mole; 4 cases of invasive mole; 10 cases of choriocarcinoma) and five cases of normal pregnancy as control group were studied for the distribution and intensity of human chorionic gonadotropin(HCG), human placental lactogen(HPL) and regnancy specific beta-1 glycoprotein(SP-1) by immunoperoxidase method. The following results were obtained. 1) The HGC was seen in the syncytiotropholasts, but not cytotrophoblasts of normal placental tissue and hydatidiform mole in all 3 grades. However, in the invasive mole and choriocarcinoma, the HCG was also seen in the cytotrophoblasts. 2) The HPL and SP-1 were only seen in the syncytiotrophoblasts in all those trophoblastic neoplasms. 3) The more malignancy was progressed, the more intensity of the HCG in the syncytiotrophoblasts was increase. Especially, it was severe intensity of positivity in the choriocarcinoma. 4) Intensity of the HPL in the syncytiotrophoblast had the tendency of increase in the invasive mole. But, it was variable in the choriocarcinoma. 5) Intensity of the SP-1 in the syncytiotrophoblast had the tendency of increase in the grade Ⅱ of hydatidiform mole. But thereafter, it was decreased or variable in more aggressive trophoblastic neoplasms, In conclusion, the HCG, HPL and SP-1 were present in the syncytiotrophoblasts of normal placental tissue and various trophoblastic neoplasms. And, the more severe hyperplasia and undifferentiation of trophoblasts were seen, the more intensity of the HCG was increased, suggesting that the HCG can be a useful tumor marker of prognosis in gestational trophpblastic neoplasms.

가족체계 진단 척도 개발 및 타당화 연구 : Minuchin의 구조적 가족치료 이론에 기초하여 On the Basis of Minuchin`s Structural Family Therapy Theory

이미옥 대한가정학회 2004 Human Ecology Research(HER) Vol.42 No.3

The purpose of this study was to develop the Family System Diagnosis Scale and to m i n e its reliability and validity The subscales of the questionnaire included scores on seven construct. In order to define constructs accurately, a careful review of Minuchin s writings, the writings of other family therapists, and relevant articles on family interaction was undertaken. A pool of 150 items was given to eight family counselors along with a description of Minuchiin s concepts. The counselors were asked to choose the category each statement fit and to rate the degree of fit using the Spoint scale. Using exploratory factor analysis confirmatory factor analysis and Linear Structural Relatimship(LJSREL.), six subdimensions of individuation and 55 items of FSDS were identified; enmeshmen disengqemant(l6 items), parent coalition generational coalition(6 items), flexibility rigidity(5 items), spouse conflict resolved unresolved(8 items). mother-child cohesion estrangement(l0 items), father-child cohesion estrangement(l0 items). 356 adolescents(ages 13-18), 356 fathers, 356 mothers in Seoul, Busan, Dague, Incheun, Dajeun, Ulsan, and Kwangju were completed the Family system Diagnosis We(FSDS). The reliability of the scale was calculated by Cronbach's a Coefficient and the total α = .94 and the calculation for each factor was .87, .60, .77, .80 and .79 respectively.