Retinoic acid induces expression of Ig germ line α transcript, an IgA isotype switching indicative, through retinoic acid receptor

Mi-Hee Park,박석래,Mi-Ra Lee,Young-Ha Kim,Pyeung-Hyeun Kim 한국유전학회 2011 Genes & Genomics Vol.33 No.1

Retinoic acid (RA) is considered to possess an activity of IgA isotype switching. Thus far, TGF-β1 is known to be the most powerful IgA isotype switch factor. To elucidate the molecular mechanisms underlying the Ig germ line (GL) α transcriptional regulation by RA, we constructed three different sizes of mouse GLα promoter reporters; short-GLα(-130/+14), middle-GLα(-448/+72) and long-GLα(-3028/+72). Based on luciferase assay, RA increased the activity of all three GLα promoter reporters by approximately 2-fold and the effect was further enhanced by TGF-β1. Overexpression of Smad3/4 increased TGF-β1-induced GLα promoter activities but had no effect on RA-induced GLα promoter activities. In order to analyze the characteristics of the RA-inducible GLα promoter region,we also constructed two mutant reporters: Smad3 binding elements (SBEs)-substituted short-GLα (short-GLα mSBE)and Runx3 binding elements (RBEs)-substituted short-GLα(short-GLα mRBE) promoter reporters. Promoter activities of the two mutant reporters to RA were comparable to that of wild type reporter, while those of the two mutant reporters to TGF-β1 were markedly diminished as compared to that of WT short-GLα. Finally, RA-induced GLα transcription was virtually disappeared by LE540, an antagonist of RA receptor (RAR). Taken together, these results suggest that RA induces GLα transcription mainly through RAR pathway, where neither Smad3/4 nor Runx3 is involved.

류마티스관절염 환자의 인슐린 저항성 결정인자

이정욱 ( Joung Wook Lee ),박영은 ( Young Eun Park ),조미라 ( Mi Ra Cho ),백승훈 ( Seung Hoon Baek ),김근태 ( Geun Tae Kim ),이준희 ( Jun Hee Lee ),김성일 ( Sung Il Kim ) 대한류마티스학회 2009 대한류마티스학회지 Vol.16 No.2

Objective: Rheumatoid arthritis (RA) is associated with an increased cardiovascular events. These may be related to insulin resistance (IR). We evaluated status of IR and analyzed the relationship between IR and clinical and laboratory characteristics in patients with RA. Methods: We examined 52 RA patients (43 females) and 52 age and sex matched healthy controls. We measured Homeostasis model assessment (HOMA) IR, calculated according to fasting serum glucose and insulin. Results: In patients, age was 50.8±10.2 years old, disease duration was 42.1±30.9 months. In controls, HOMA IR was 0.62±0.30 and in patients, it was 1.28±0.50. This difference was highly significant (p<0.001). Patients with early RA (disease duration is less than 36 months) were 28, and established RA (more than 36 months) were 24. HOMA IR was significantly higher in patients with established RA (1.42±0.45) than those with early RA (1.16±0.45) (p=0.03), and significantly correlated with disease duration (r=0.36, p=0.01), BMI (r=0.36, p<0.001), total cumulative prednisolon dose (r=0.34, p=0.01). Disease duration and BMI were independent predictors for HOMA IR (p<0.01, p=0.03). Conclusion: In patients with RA, IR measured by HOMA IR was more significantly increased than that of healthy control and significantly correlated with disease duration, BMI, and total cumulative prednisolon dose; however, the determinants of HOMA IR in RA patients were disease duration and BMI.

류마티스관절염과 만성 호산구성 폐렴에 동반된 호산구성 흉막삼출

지용관 ( Yong Gwan Jee ),라상호 ( Sang Ho Ra ),박유미 ( Yu Mi Park ),차재황 ( Jae Whang Cha ),강용석 ( Yong Seok Kang ),박정하 ( Jeong Ha Park ),강태영 ( Tae Young Kang ) 대한류마티스학회 2013 대한류마티스학회지 Vol.20 No.5

We describe a 48-year-old man with family history of rheumatoid arthritis (RA) affected by chronic eosinophilic pneumonia (CEP) with severe peripheral eosinophilia. CEP might develop as a complication of longstanding active RA. The patient with 5 months history of seropositive RA and chronic respiratory symptoms, alveolar and blood eosinophilia, peripheral pulmonary infiltrates and pleural effusion on chest imaging. The lung may be involved as an extraarticular manifestation of RA. However, CEP is not recognized as a typical lung manifestation of RA, and the two diseases rarely coexist. The effusion was an eosinophil predominant exudates and was characterized by low pH, and glucose level and high lactic dehydrogenase. The patient responded rapidly to combination of steroids and disease modifying anti-rheumatic drugs.

3′‐Sialyllactose as an inhibitor of p65 phosphorylation ameliorates the progression of experimental rheumatoid arthritis

Kang, Li‐,Jung,Kwon, Eun‐,Soo,Lee, Kwang Min,Cho, Chanmi,Lee, Jae‐,In,Ryu, Young Bae,Youm, Tae Hyun,Jeon, Jimin,Cho, Mi Ra,Jeong, Seon‐,Yong,Lee, Sang‐,Rae,Kim, Wook,Yang John Wiley and Sons Inc. 2018 British journal of pharmacology Vol.175 No.23

<P><B>Background and Purpose</B></P><P>3′‐Sialyllactose (3′‐SL) is a safe compound that is present in high levels in human milk. Although it has anti‐inflammatory properties and supports immune homeostasis, its effect on collagen‐induced arthritis (CIA) is unknown. In this study, we investigated the prophylactic and therapeutic effect of 3′‐SL on the progression of rheumatoid arthritis (RA) in <I>in vitro</I> and <I>in vivo</I> models.</P><P><B>Experimental Approach</B></P><P>The anti‐arthritic effect of 3′‐SL was analysed with fibroblast‐like synoviocytes <I>in vitro</I> and an <I>in vivo</I> mouse model of CIA. RT‐PCR, Western blotting and ELISA were performed to evaluate its effects <I>in vitro</I>. Histological analysis of ankle and knee joints of mice with CIA was performed using immunohistochemistry, as well as safranin‐O and haematoxylin staining.</P><P><B>Key Results</B></P><P>3′‐SL markedly alleviated the severity of CIA in the mice by reducing paw swelling, clinical scores, incidence rate, serum levels of inflammatory cytokines and autoantibody production. Moreover, 3′‐SL reduced synovitis and pannus formation and suppressed cartilage destruction by blocking secretion of chemokines, pro‐inflammatory cytokines, https://en.wikipedia.org/wiki/Matrix_metalloproteinases and osteoclastogenesis <I>via</I> NF‐κB signalling. Notably, phosphorylation of p65, which is a key protein in the NF‐κB signalling pathway, was totally blocked by 3′‐SL in the RA models.</P><P><B>Conclusions and Implications</B></P><P>3′‐SL ameliorated pathogenesis of CIA by suppressing catabolic factor expression, proliferation of inflammatory immune cells and osteoclastogenesis. These effects were mediated <I>via</I> blockade of the NF‐κB signalling pathway. Therefore, 3′‐SL exerted prophylactic and therapeutic effects and could be a novel therapeutic agent for the treatment of RA.</P>

마우스에서 조합시험법에 의한 고려홍삼의 생식발생독성 평가

김미라, 박동선, 장자영, 신선희, 이예은, 백인정, 연정민, 이세라 , 남상섭, 황방연, 노재섭, 김순선, 조대현, 남상윤, 이범준, 김윤배, 윤영원 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.1

본문참조

동북아 협력 네트워크와 지방의 역할

라미경 호남대학교 인문사회과학연구소 2007 인문사회과학연구 Vol.17 No.-

국제환경의 변화에 따라 동북아 역내 간에 있어서 긴밀한 교류 협력의 필요성은 더욱 절실하게 제기되고 있다. 더 나아가 동북아 지역의 시민들이 공동의 평화와 번영을 함께 추구하고 영위할 수 있는 지역공동체의 설립이 중요한 과제로 떠오르고 있다. 이에 본 연구는 동북아 지역공동체 형성이라는 상황을 지방간의 관계를 중심으로 분석하고자 한다. 동북아 지역의 지방들이 국경을 넘어 어떻게 교류협력하고 있는가,서로 쟁점들은 무엇인가 그리고 경제적 상호보완성과 지리적 근접성을 살리면서 앞으로 지역공동체 형성에 지방들이 기여할 수 있을 것인가 등에 대한 연구는 시의적절하다고 생각한다. 왜냐하면 동북아 지역공동체와 이 지역의 협력이 중앙정부 차원에서만 진행되고 있지 않기 때문 이다. 또한 지방끼리의 협력과 전망에 대한 연구 성과가 미비하기 때문에 본 연구를 통해 이 분야연구에 기여하고자 하는 데 목적이 있다. This paper discusses the Roles of Local Government and Cooperative Network In Northwest Asia. It follows in change of international environment and it is proposed the necessity of the cooperation to Northeast Asia inside of area. The research which is a Northeast Asia area community it analyzes the relationship of region for in the center and it does to sleep. The regions of the Northeast Asia area will go over the border and the flow cooperative, with each other are the issues will be what how and to save an economic trade name complement result geography neighboring characteristic and the regions will be able to contribute in the next area community formation is the research against the back thinks that it is right time and appropriate. Because the Northeast Asia area community and cooperation of this region only central government dimension, it is because is not advanced.

The efficacy of bench-step aerobic program in reducing some cardiovascular risk factors in overweight or obese women

이미라,김완수 대구대학교 인문과학연구소 2008 人文科學硏究 Vol.32 No.-

본 연구의 목적은 12주 스텝운동 프로그램이 과체중(23 ㎏/㎡≤체질량지수<25 ㎏/㎡) 및 비만(체질량지수≥25 ㎏/㎡) 여성의 심혈관 위험 요인(비만관련 지표와 혈당 및 지질성분)에 미치는 효과를 알아보기 위한 것이다. 선발된 25명의 과체중 및 비만 여성 중 프로그램에 90% 이상 참여하고 프로그램 전과 후에 이루어진 검사를 수행한 15명의 프로그램 수행 전과 후의 비만관련 지표(체중, 체질량지수, 체지방율 및 총체지방), 혈당, 중성지방 그리고 고밀도지단백콜레스테롤을 비교하였다. 본 프로그램은 주 3회, 12주 동안 이루어졌고, 45분에서 60분 동안 예비심박수의 40/50%에서 50/60%의 운동 강도로 진행되었다. 운동량은 건강상의 이점 획득 목적으로 현재 권고되는 최소 운동량에 근거하여 결정되었다. 12주 스텝운동 프로그램 후 대상자의 체지방율이 유의수준에 가깝게 감소되었고(T=2.121, P=.052), 체중, 체질량지수와 체지방이 유의하게 감소되어(T=5.270, P=.000; T=2.658, P=.019; T=3.661, P=.003), 전반적으로 비만관련 지표에 미치는 효과가 지지되었다. 혈당(T=1.850, P=.087), 중성지방(T=-1.249, P=.234) 및 고밀도지단백콜레스테롤(T=.084, P=.934)은 유의한 변화를 보이지 않았다. 본 연구결과는 선행 연구결과들과 함께 12주 스텝운동 프로그램이 전반적으로 비만관련 지표를 유의하게 감소시킴으로써 과체중 및 비만 여성의 심혈관 질환 위험을 감소시킬 수 있는 효과적인 운동 프로그램이 될 수 있음을 보여주고 있다. 반면 혈당과 혈중 지질성분 개선을 위해서는 운동량의 증가 또는 근량 증가 및 내장지방 감소 효과를 초래할 수 있는 운동 프로그램으로의 수정 등과 같은 전략들이 부가되어야 한다는 것을 시사하고 있다. 그러나 본 연구 포함 스텝운동 프로그램의 심혈관 위험 감소 효과를 검증한 현재까지의 연구들은 연구 설계상의 제한점을 갖고 있고, 따라서 일반화를 위한 무작위화되고 통제된 연구가 필요하다.

복부비만 여성 대상 걷기 운동 프로그램의 비만관련 지표 및 심폐체력 개선 효과

이미라,김완수 김천과학대학 2007 김천과학대학 논문집 Vol.33 No.-

The purpose of this study was to investigate the effects of a 12-wk brisk walking program consisted of the exercise amount, which is comparable with the current minimum physical activity recommendation associated with health benefits, on obesity-related indices (body weight, body mass index, waist circumference, waist-to-bip ratio, body fat percent, and total body fat) and aerobic fitness in Korean women with abdominal obesity. The subjects were 11 middle-aged women with abdominal obesity (waist circumference ≥80 cm). The intervention consisted of walking for 45-60 min per day at an intensity of 40(50) to 60(65)% of heart rate reserve(HRR) for 3 days per week Subjects of this study had decreases in obesity-related indices overall. Decreases in body weight, waist-to-hip ratio, body mass index, waist circumference, and total body fat were statistically significant. Aerobic fitness was increased after the intervention and this increase was close to statistical significance. A 12-wk brisk walking program consisted of the exercise amount, which is comparable with the current minimum physical activity recommendation associated with health benefits, could be an effective strategy to reduce obesity-related indices in middle-aged Korean women with abdominal obesity. Whether higher amount of exercise is required to promote significant improvement in aerobic fitness in this population needs to be investigated in well-controlled further studies.

치과에서 사용되는 필름 현상액에 대한 품질 평가 연구

한미라,강병철 전남대학교 치과대학 1999 전남치대논문집 Vol.11 No.1

The purpose of this study was to compare the qualities of the four different processing chemicals(solutions). With EP 21 films(Ektaspeed plus film, Kodak Co., USA), nine unexposed and nine exposed films of a step wedge were processed utilizing automatic film processor(XR 24, Durr Co., Germany) for 5 days. During 5 days, the total number of processed films including out-patient's intraoral films were about 400-500 for each brand. Base plus fog density, film density, contrast of processed films were measured with densitometer(model 07-443 digital densitometer, Victoreen Co., USA). These measurements were analyzed for comparison. The results were as follows, 1. For the base plus fog density, there was significant difference among the four chemicals(p<0.05). The sequence of the base plus fog densities was in ascending order by Kodak, X-dol 90, Agfa and Konica. 2. For the film density, all chemicals showed useful range of photographic densities(0.25-2.5). The sequence of the film densities was in ascending order by Kodak, X-dol 90, Konica and Agfa. But there was no statistically significant difference of film density between X-dol and Kodak (p<0.05). 3. The sequence of the contrasts was in ascending order by Konica, X-dol 90, Kodak and Agfa. But there was no statistically significant difference of contrast between X-dol and Konica (p<0.05). These results indicated that the four processing chemicals had the clinically useful film density and contrast, but only Kodak processing chemical had useful base plus fog density.

초등학교 6학년 아동의 흡연에 관한 연구

박미라,박인숙 충남대학교 간호과학연구소 2003 충남대 간호학술지 Vol.6 No.1

The purpose of this study was to make a comparative analysis of smoking knowledge, attitude and behavior among children in their six year in elementary school to serve as a basis for the development of successful smoking prevention education program. The subjects were 600 boy and girl students in the sixth grade randomly selected from five elementary schools in north Chungcheong province. Data were collected for six days from September 2 through 7, 2002. To assess subjects smoking knowledge and attitude, Choi Hye-yeoung (2000)'s modified version of WHO(1982)'s Guidelines for the content of tobacco smoking surveys of the general population was again altered by this researcher for use in this study. The collected data were analyzed with SAS program. The frequency, percentage, mean and standard deviation were calculated, and t-test Chi-square test, ANOVA, and Logistics regression procedures were employed. The brief findings of this study were as 1) Out of the 600 students investigated, 21.83 percent(131 students) had a smoking experience, and among these students, 1.83 percent(11) smoked at present. 2) The collective average in smoking knowledge was 13.04 out of 19 points. The smoking-experience group got a mean of 12.78, and the non-experienced group got a mean of 13.12. The latter group had a slightly better knowledge than the former, but the difference between the two was insignificant(p=0.416). 3) The collective average in smoking attitude was 34.45, out of 40 points. The non-experienced group got a mean of 35.41, and the experienced group got a mean of 30.77. The former group had a more desirable attitude than the latter, and the gap between the two groups was very significant(p=0.000). 4) The smoking knowledge was positively correlated to the smoking attitude(r=0.24, p=0.000). 5) As for connections between smoking knowledge and general characteristics, school grades(F =10.97, p=0.000), the sources of smoking information(F=3.00, p=0.000), father educational background(F=4.06, p=0.003) and gender(t =-2.31, p=0.21) made a significant difference to the smoking knowledge. 6) Concerning relations between smoking attitude and general characteristics, the smoking attitude was different according to gender (t = -4.53, po=0.000), school grades(F=7.96, p=0.000), smoking or non- smoking of siblings(F=5.86, p=0.000), presence or absence of smoking friends(F=3.07, p=0.003), family atmosphere(F=4.87, p=0.008), experience of going on errands to buy a pack of cigarettes(t = -2.50, p=0.012), father's smoking or nonsmoking(F=4.87, p=0.026), and parent marital status (F=2.40, p=0.049). 7) Regarding connections between smoking experience and general characteristics, their smoking experience was different according to gender(x2=21.38, p=0.000), presence or absence of smoking friends (x2=55.81, p=0.000), smoking or nonsmoking of siblings(x2 = 15.11, p=0.004), experience of going on errands to buy a pack of cigarette(x2=7.50, p=0.006), mother's smoking or nonsmoking(x2 = 13.34, p=0.009), female relative's smoking or nonsmoking(x2 = 11.39, p=0.022), and mother's job (x2=4.64, p=0.031). 8) The factors associated with the smoking experience were the number of smoking friends(Wald x2=29.62, p=0.000) and smoking attitude(Wald x2=42.54, p=0.000).