Transcriptional activation of hypoxia-inducible factor-1α by HDAC4 and HDAC5 involves differential recruitment of p300 and FIH-1

Seo, Hee-Won,Kim, Eun-Jin,Na, Hyelin,Lee, Mi-Ock Elsevier 2009 FEBS letters Vol.583 No.1

<P><B>Abstract</B></P><P>The interplay between hypoxia-inducible factor-1α (HIF-1α) and histone deacetylase (HDACs) have been well studied; however, the mechanism of cross-talk is unclear. Here, we investigated the roles of HDAC4 and HDAC5 in the regulation of HIF-1α function and its associated mechanisms. HDAC4 and HDAC5 enhanced transactivation by HIF-1α without stabilizing HIF-1α. HDAC4 and HDAC5 physically associated with HIF-1α through the inhibitory domain (ID) that is the binding site for factor inhibiting HIF-1 (FIH-1). In the presence of these HDACs, binding of HIF-1α to FIH-1 decreased, whereas binding to p300 increased. These results indicate that HDAC4 and HDAC5 increase the transactivation function of HIF-1α by promoting dissociation of HIF-1α from FIH-1 and association with p300.</P><P><B>Structured summary:</B></P><P>MINT-6802187:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>FIH1</I> (uniprotkb:Q9NWT6) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802058:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>HDAC4</I> (uniprotkb:P56524) by <I>pull down</I> (MI:0096)</P><P>MINT-6802021:</P><P><I>HIF1 alpha</I> (uniprotkb:Q61221) <I>physically interacts</I> (MI:0218) with <I>HDAC4</I> (uniprotkb:P56524) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802036:</P><P><I>HIF1 alpha</I> (uniprotkb:Q61221) <I>physically interacts</I> (MI:0218) with <I>HDAC5</I> (uniprotkb:Q9UQL6) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802102:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>HDAC5</I> (uniprotkb:Q9UQL6) by <I>pull down</I> (MI:0096)</P><P>MINT-6802121, MINT-6802156:</P><P><I>P300</I> (uniprotkb:Q09472) <I>physically interacts</I> (MI:0218) with <I>HIF1 alpha</I> (uniprotkb:Q16665) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P>

Microbial Differentiation on Chemical Properties of Paddy Soils in Reclaimed Tidal Lands at Western-coast Area of Korea

Mi-Na Park,Kwang-Min Yang,Jin-Hee Ryu,Tongmin Sa,Joon-Ho Choi 한국토양비료학회 2016 한국토양비료학회지 Vol.49 No.4

The scientific information on the microbial differentiation according to the changes in chemical properties of paddy soil in reclaimed tidal lands is not enough to understand the reclamation processes. The changes in microflora based on the chemical properties of paddy soils at the same sites of reclaimed tidal lands (21 samples from Nampo, Ewon, Sukmoon and Shihwa sites) were investigated in 2013 and 2015. In general, organic matter in paddy soils increased whereas pH decreased with the reclamation time. The electrical conductivities (EC) of soil samples were closely related to the exchangeable Na+. With an increases in EC of paddy soils from 0.39 to 48.9 dS m-1, the ratios (%) of halotolerant and halophilic bacteria to mesophilic bacteria proportionally increased from 0.2% to 102,000%. The population of halotolerant and halophilic bacteria in total microflora was also differentiated with the changes in EC of the same sites from reclaimed tidal soils within 2 years. The population of mesophilic bacteria decreased with an increase in EC above 5 dS m-1. The microflora including halotolerant and halophilic bacteria could be a candidate as a biological parameter in evaluating the reclamation processes in addition to the chemical index of EC.

A Na⁺-coupled C₄-dicarboxylate transporter (Asuc_0304) and aerobic growth of <i>Actinobacillus succinogenes</i> on C₄-dicarboxylates

Rhie, Mi Na,Yoon, Hyo Eun,Oh, Hye Yun,Zedler, Sandra,Unden, Gottfried,Kim, Ok Bin Society for General Microbiology 2014 Microbiology Vol.160 No.7

<P><I>Actinobacillus succinogenes</I>, which is known to produce large amounts of succinate during fermentation of hexoses, was able to grow on C<SUB>4</SUB>-dicarboxylates such as fumarate under aerobic and anaerobic conditions. Anaerobic growth on fumarate was stimulated by glycerol and the major product was succinate, indicating the involvement of fumarate respiration similar to succinate production from glucose. The aerobic growth on C<SUB>4</SUB>-dicarboxylates and the transport proteins involved were studied. Fumarate was oxidized to acetate. The genome of <I>A. succinogenes</I> encodes six proteins with similarity to secondary C<SUB>4</SUB>-dicarboxylate transporters, including transporters of the Dcu (C<SUB>4</SUB>-dicarboxylate uptake), DcuC (C<SUB>4</SUB>-dicarboxylate uptake C), DASS (divalent anion : sodium symporter) and TDT (tellurite resistance dicarboxylate transporter) family. From the cloned genes, Asuc_0304 of the DASS family protein was able to restore aerobic growth on C<SUB>4</SUB>-dicarboxylates in a C<SUB>4</SUB>-dicarboxylate-transport-negative <I>Escherichia coli</I> strain. The strain regained succinate or fumarate uptake, which was dependent on the electrochemical proton potential and the presence of Na<SUP>+</SUP>. The transport had an optimum pH ~7, indicating transport of the dianionic C<SUB>4</SUB>-dicarboxylates. Transport competition experiments suggested substrate specificity for fumarate and succinate. The transport characteristics for C<SUB>4</SUB>-dicarboxylate uptake by cells of aerobically grown <I>A. succinogenes</I> were similar to those of Asuc_0304 expressed in <I>E. coli</I>, suggesting that Asuc_0304 has an important role in aerobic fumarate uptake in <I>A. succinogenes</I>. Asuc_0304 has sequence similarity to bacterial Na<SUP>+</SUP>-dicarboxylate cotransporters and contains the carboxylate-binding signature. Asuc_0304 was named SdcA (<U>s</U>odium-coupled C<SUB>4</SUB>-<U>d</U>i<U>c</U>arboxylate transporter from <I><U>A</U></I>. <I>succinogenes</I>).</P>

효소분해와 염과 당 및 항산화 작용 상승제의 첨가에 의한 계피 추출액의 특성 변화

김나미(Na Mi Kim),도재호(Jae Ho Do),이종수(Jong Soo Lee),김우정(Woo Jung Kim) 한국응용생명화학회 1994 Applied Biological Chemistry (Appl Biol Chem) Vol.37 No.4

The dried cinnamon was extracted with enzymes, salts, sugars and additives in order to find the most effective extraction material. Enzymatic hydrolysis of cinnamon suspension with cellulase, hemicellulase, pectinase, β-1.4-glucosidase, tannase and lipase showed a little increase of their cinnamic aldehyde contents. Solid yield, antioxidant activity and degree of browning were increased in hemicellulase treatment. Acid and alkali extraction of cinnamon showed a some increase in solid yields and antioxidant activity was increased by addition of glucose and Na-ascorbate. Cinnamic aldehyde contents and degree of browning were increased in extraction with Na-citrate addition.

한강하구 장항습지 하천숲의 군집특성

나미연 ( Mi-yeon Na ),오충현 ( Choong-hyeon Oh ) 한국환경생태학회 2021 한국환경생태학회 학술대회지 Vol.2021 No.1

장항습지는 강둑이 없는 자연형 기수역에 속하며, 생물다양성 측면에서 가치가 높은 수변 습지이다. 한강과 임진강과 합류하여 서해로 유입되는 지점부터 장항습지까지 한강하구에 속하며 일부지역은 1953년 비무장지대(DMZ)로 설정되고, 장항습지는 50여년간 군사시설보호지역으로 지정되어 민간인 출입이 통제되었다. 습지 보전 상태가 양호하고, 하천을 따라 길게 형성된 버드나무 군락은 철새와 멸종위기종의 서식처가 되고 있다. 최근 고양시 장항습지 발전전략 수립연구에서 수변상 육상화를 방지하고 버드나무군락의 생물다양성과 역동성을 유지하기 위해 습지의 물흐름에 대한 복원의 필요성을 제기되었으나, 육화 현황과 식생에 대한 선행 연구가 미흡한 실정이다. 본 연구는 한강하구 퇴적작용에 의한 지형변화를 파악하고, 버드나무 군락의 군집구조를 분석하여 육화 진행 현황과 원인을 분석하여 향후 습지보전 관리 방안과 복원 방향을 위한 기초 자료의 활용을 위해 수행되었다. 장항습지는 과거 행정구역상 옛 고양군 지도읍에 속하며, 1970년대 ‘사미섬’의 모습은 두 개의 하중도 형태이며, 1974년 항공사진과 구글어스(Googl Earth Map)의 1985년 위성사진을 통해 ‘사미섬’의 형태를 확인할 수 있다. 1988년 신곡수중보 설치 이후에는 수중보 아래쪽에 유속이 느려지면서 퇴적량이 늘어 하나의 섬으로 연결되었으며, 1990년 자유로건설 등 인위적인 요인으로 육지면적이 증가하여 자유로를 따라 현재와 같은 장항습지의 형태로 사주가 발달하였다. 2000년대 이후 장항습지 면적은 계속해서 증가하고 버드나무 군락이 형성되었다. 버드나무 군락을 크게 6개의 구역으로 구분한 각 30개의 조사구를 100㎡ 면적의 방형구법(Quadrat)을 이용하여 교목, 아교목, 관목의 세 개 층위별 개체밀도와 흉고직경(DBH), 수관폭을 조사하여 상대우점치(Importance Value)와 평균상대 우점치(Mean Importance Value)를 분석하였다. Brown-Blanquet(1913) 방법으로 각 방형구내 출현하는 식물종을 조사하여 Shannon의 최대종다양도(H´max), 우점도(Dominance)와 균재도(Evenness)를 산출하여 비교하였다. TWINSPAN 기법으로 군락을 분류하여 군집특성을 파악하고, 각 조사구에서 수변까지의 거리와 대표 수종 MIV의상관분석(Correlation analysis)과 회귀분석(Regression analysis)을 진행하였다. 각 조사구의 흉고직경(DBH)별 개체수 분포를 통해 식물의 수령 및 수목 군집의 천이 과정을 파악하였고, 환경요인 분석으로 조사시점의 과거 10여년간 연도별 평균수위와 개화기 이전 1월~3월의 강수량, 군락별 토양입도, 유기물과 무기물 등 토양 성분을 분석하였다. TWINSPAN 기법을 통한 장항습지 군락 분류는 크게 선버들군락, 버드나무-선버들군락, 버드나무-능수버들군락으로 나뉜다. 수변거리와 버드나무 MIV는 43%의 양의 상관관계를 가지며, 선버들과 버드나무의 MIV는 93%의 강한 음의 상관관계가 있음을 확인하였다. 버드나무 MIV와 수변거리와의 회귀분석에서 p-value가 0.01906으로 0.05보다 작으므로 유의한 값을 가진다. 즉, 한강 수변상에서 가까운 곳에 선버들이 우점하고, 수변에서 멀어질수록 버드나무가 우점한다는 결론을 도출하였다. 전 조사구의 DBH 분포는 버드나무가 10cm~30cm 범위내에 집중적으로 분포하고, 선버들 DBH는 2cm~ 50cm 이상까지 넓게 분포하였다. 선버들 우점하는 조사 구에서 DBH 30cm와 42cm의 대경목 두 개체와 직경 14cm~23cm의 8개의 분지가 연결된 DBH가 140cm인 고령목이 확인되었다. 이를 통해 장항습지의 최초 선구수종은 선버들이며, 점차적으로 버드나무가 이입되었다는 것을 알 수 있다. 조사연도로부터 과거 5년간 강수량과 수위가 감소하였고, 이에 따라 선버들의 고사목 비율이 급속도로 늘어나면서 버드나무가 세력을 확장한 것으로 판단된다. 또한 관목층에서 찔레꽃, 오갈피나무, 쥐똥나무 등 산림 수종의 출현하는 것으로 보아 육화가 이미 진행됨을 확인하였다. 버드나무속 조사구내 출현하는 초본층 포함한 식물종을 중심으로 진행한 종다양도 분석에서 선버들이 우점한 군락내 우점도(Dominance)가 높게 나타난 반면, 버드나무가 우점하는 군락에서 국화과, 마디풀과, 석죽과 등 다양한 초본이 출현하여 우점도가 낮게 나타났다. 이는 육화 진행에 따른 귀화식물, 생태계교란식물 등 외부 이입 종이 증가한 것으로 보인다. 수목생장을 위한 토양성분 적정함량 기준치의 염화나트륨(NaCl) 함량은 0.05%이하이고, 선버들 고사목 비율이 높은 세 조사구에서 기준치보다 높게 나타났다. 나트륨 양이온치환용량(Na+)의 경우, 선버들이 우점하는 두 조사구에서는 낮게 나타나고, 선버들 고사목 비율이 높은 5개의 조사구에서 염분 농도가 상대적으로 높은 것을 확인하였다. 기수역 특성상 버드나무속 군락내 초본층에서 모새달, 산조풀, 갈대 등의 염생식물이 출현하여 염분 농도를 대략적 예측이 가능하였다. 기상요인과 수위변화로 수분함량이 줄어들고, 육화가 진행됨에 따라 나트륨성분은 토양속에 그대로 잔재한 것으로 추정된다. 육화 방지를 위해서는 식생 및 식물상 조사를 바탕으로 습지내 갯골과 물골을 복원하거나, 염분 농도가 낮은 관개수를 공급하는 것이 효과적일 것이다. 본 연구를 통해 장항습지 하천변 수목군집구조는 선버들 군락에서 버드나무 군락으로 천이가 진행되고, 선버들 고사목 비율이 늘어남에 따라 이미 육화가 심각한 단계에 이르렀음을 확인하였다. 최근 습지보전법에 추가된 하천 수생태계 보전과 습지의 육역화 방지의 내용이 추가됨에 따라 육화 방지를 위한 체계적인 관리 방안과 구체적인 대책 마련이 시급하다.

Gintonin facilitates catecholamine secretion from the perfused adrenal medulla

Seung-Yeol Na,Ki-Hwan Kim,Mi-Sung Choi,Kang-Su Ha,Dong-Yoon Lim 대한생리학회-대한약리학회 2016 The Korean Journal of Physiology & Pharmacology Vol.20 No.6

The present study was designed to investigate the characteristics of gintonin, one of components isolated from Korean Ginseng on secretion of catecholamines (CA) from the isolated perfused model of rat adrenal gland and to clarify its mechanism of action. Gintonin (1 to 30 μg/ml), perfused into an adrenal vein, markedly increased the CA secretion from the perfused rat adrenal medulla in a dose-dependent fashion. The gintonin-evoked CA secretion was greatly inhibited in the presence of chlorisondamine (1 μM, an autonomic ganglionic bloker), pirenzepine (2 μM, a muscarinic M₁ receptor antagonist), Ki14625 (10 μM, an LPA_1/3 receptor antagonist), amiloride (1 mM, an inhibitor of Na⁺/Ca²⁺ exchanger), a nicardipine (1 μM, a voltage-dependent Ca²⁺ channel blocker), TMB-8 (1 μM, an intracellular Ca²⁺antagonist), and perfusion of Ca²⁺-free Krebs solution with 5mM EGTA (a Ca²⁺chelater), while was not affected by sodium nitroprusside (100 μM, a nitrosovasodialtor). Interestingly, LPA (0.3~3 μM, an LPA receptor agonist) also dose-dependently enhanced the CA secretion from the adrenal medulla, but this facilitatory effect of LPA was greatly inhibited in the presence of Ki 14625 (10 μM). Moreover, acetylcholine (AC)-evoked CA secretion was greatly potentiated during the perfusion of gintonin (3 μg/ml). Taken together, these results demonstrate the first evidence that gintonin increases the CA secretion from the perfused rat adrenal medulla in a dose-dependent fashion. This facilitatory effect of gintonin seems to be associated with activation of LPA- and cholinergic-receptors, which are relevant to the cytoplasmic Ca²⁺ increase by stimulation of the Ca²⁺ influx as well as by the inhibition of Ca²⁺ uptake into the cytoplasmic Ca²⁺ stores, without the increased nitric oxide (NO). Based on these results, it is thought that gintonin, one of ginseng components, can elevate the CA secretion from adrenal medulla by regulating the Ca²⁺ mobilization for exocytosis, suggesting facilitation of cardiovascular system. Also, these findings show that gintonin might be at least one of ginseng-induced hypertensive components.

Polyphenols of Rubus coreanum Inhibit Catecholamine Secretion from the Perfused Adrenal Medulla of SHRs

Byung-Sik Yu,Duck-Mi Na,Mi-Young Kang,Dong-Yoon Lim 대한생리학회-대한약리학회 2009 The Korean Journal of Physiology & Pharmacology Vol.13 No.6

The present study was attempted to investigate whether polyphenolic compounds isolated from wine, which is brewed from Rubus coreanum Miquel (PCRC), may affect the release of catecholamines (CA) from the isolated perfused adrenal medulla of the spontaneously hypertensive rats (SHRs), and to establish its mechanism of action. PCRC (20∼180Ռg/ml) perfused into an adrenal vein for 90 min relatively dose-dependently inhibited the CA secretory responses to ACh (5.32 mM), high K⁺ (56 mM), DMPP (100ՌM) and McN-A-343 (100ՌM). PCRC itself did not affect basal CA secretion (data not shown). Also, in the presence of PCRC (60Ռg/ml), the CA secretory responses to veratridine (a selective Na⁺ channel activator (10ՌM), Bay-K-8644 (a L-type dihydropyridine Ca²⁺ channel activator, 10ՌM), and cyclopiazonic acid (a cytoplasmic Ca²⁺-ATPase inhibitor, 10ՌM) were significantly reduced, respectively. In the simultaneous presence of PCRC (60Ռg/ml) and L-NAME (an inhibitor of NO synthase, 30ՌM), the inhibitory responses of PCRC on the CA secretion evoked by ACh, high K⁺, DMPP, and Bay-K-8644 were considerably recovered to the extent of the corresponding control secretion compared with that of PCRC-treatment alone. The level of NO released from adrenal medulla after the treatment of PCRC (60Ռg/ml) was greatly elevated compared with the corresponding basal level. Taken together, these results demonstrate that PCRC inhibits the CA secretion from the isolated perfused adrenal medulla of the SHRs evoked by stimulation of cholinergic receptors as well as by direct membrane-depolarization. It seems that this inhibitory effect of PCRC is mediated by blocking the influx of calcium and sodium into the adrenal medullary chromaffin cells of the SHRs as well as by inhibition of Ca²⁺ release from the cytoplasmic calcium store at least partly through the increased NO production due to the activation of NO synthase.

Mycobacterium intracellulare에 의한 요추부 척추염 1예

장은영,김미영,김정욱,송은희,백관미,정용필,성흥섭,김미나,김양수,우준희,이상호 대한감염학회 2007 감염과 화학요법 Vol.39 No.3

비결핵성 미코박테리아에 의한 척추염에 대한 증례 보고가 국내에서는 아직 없었다. 저자들은 기저질환이 없는 환자에서 M. intracellulare에 의한 척추염을 rpoB 유전자에 대한 PCR-RFLP 방법을 통해 진단하였고 수술적 치료와 3제 병합 항균 요법으로 성공적인 임상 경과를 보인 1예를 경험하였기에 보고하는 바이다. We report a case of vertebral osteomyelitis due to Mycobacterium intracellulare in a 60-year-old man without predisposing conditions. He was successfully treated with surgical excision of the inflamed tissue and combined antimycobacterial therapy consisting of clarithromycin, ethambutol, and rifampin. In this case, polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP) based on the rpo B gene of cultured isolates allowed rapid identification of M. intracellulare. Although very rare, nontuberculous mycobacteria (NTM) should be suspected as a causative pathogen of vertebral osteomyelitis. To the best of our knowledge, this is the first case of vertebral osteomyelitis caused by NTM reported in the Korean literature.

Functional maturation of lamina propria dendritic cells by activation of NKT cells mediates the abrogation of oral tolerance

Chang, Jae-Hoon,Lee, Jung-Mi,Youn, Hyun-Jun,Lee, Kyoo-A,Chung, Yeonseok,Lee, Ah-Young,Kweon, Mi-Na,Kim, Ho-Youn,Taniguchi, Masaru,Kang, Chang-Yuil WILEY-VCH Verlag 2008 European journal of immunology Vol.38 No.10

<P>We previously showed that although systemic administration of α-galactosylceramide (αGalCer) or agonistic anti-CD40 induced functional maturation of dendritic cells (DC) in mesenteric lymph nodes, only the former treatment succeeded in breaking the induction of oral tolerance. In this study, we looked for the essential factor responsible for the disruption of oral tolerance. We found that lamina propria (LP)-DC was responsible for the oral OVA presentation and that Peyer's patch was not essential for the induction of oral tolerance. Therefore, we investigated the role of LP-DC. Treatment with αGalCer but not with anti-CD40 induced the full maturation of LP-DC at an early time point. This functional activation of LP-DC was mediated by strong activation of NKT cells that reside abundantly in the small intestinal lamina propria (SI-LP) and interferon-γ partially contributed to the LP-DC activation. LP-DC isolated from αGalCer-treated OVA-fed mice induced the differentiation of naïve CD4<SUP>+</SUP> T cells into Th1 and Th2 and was associated with the reduced Foxp3<SUP>+</SUP> population. In contrast, LP-DC isolated from anti-CD40-treated OVA-fed mice failed to generate Th cell differentiation but induced more Foxp3<SUP>+</SUP> CD4<SUP>+</SUP> T cells. Our results demonstrate that triggered by NKT cells in SI-LP, functional maturation of Ag-capturing DC from SI-LP is necessary for the abrogation of oral tolerance induction.</P>

反應性染料에 의한 綿織物의 捺染에 關한 硏究

李年純,朴惠羅,羅美香 영남대학교 자원문제연구소 1991 資源問題硏究 Vol.10 No.-

In order to investgate the change of dye adsorption and printing effect by alkali concentration steaming time, and dyeing paste, cotton fabric was printed with the reactive dyes. The results are as follows : 1. The most effect of dye adsorption showed at 0.5% Na2CO₃to dyeing paste for C. I. Reactive Blue28 and 1% Na2CO₃for Blue 198. 2. The effect of dye adsorption was practically unchanged according to the increase of steaming time for printing was 10 min. 3. The most effect of printing showed at 3% paste to dyeing paste. 4. The colorfastness to laundering of each dye was excellent and the lightfastness was good, but the colorfastness to perspiration was poor more or less. 5. It was found that the colorfastness was not influenced by alkali concentration and steaming time.