Serial values for hematologic and biochemical analysis after myocardial infarction in rats

( Mi Jin Lee ),( Hyun Jin Tae ),( Ying Hua Li ),( Do Hyeon Yu ),( In Ae Han ),( Seok Won Lee ),( Dong Choon Ahn ),( In Shik Kim ),( Jin Ho Park ) 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.2

To diagnose acute myocardial infarction(MI), many cardiac markers have been used in hematologic and biochemical analysis, and many studies have been published for hema-tologic and biochemical analysis associated with human acute MI. However, after occurrence of acute MI, the serial investigation for values in hematologic and bioche-mical analysis including chronic MI has rarely been performed. To observe the change of the serial values in hematologic and biochemical analysis, we induced artificial MI. The left main descending artery(LMDA) of the left coronary artery was ligated during the progression(day 1, 3, 5, 7, 14 and 30) of MI. Total 66 Sprague-Dawley rats were divided into the sham group(n=24, thoracotomy without LMDA ligation) and the experimental(MI) group(n=42, with LMDA ligation). And all individual in each group was sacrified at day 1, 3, 5, 7, 14 and 30 for the hematologic and biochemical analysis. In comparison of hematologic analysis between the sham and MI groups, the mean values of red blood cell(RBCs), hemoglobin and hematocrit(HCT) showed a steady increase. In biochemical analysis, the mean values of glucose, choles-terol, total creatine kinase(CK) and isoenzyme MB, and lactate dehydrogenase(LDH) were increased in all MI groups compared with the sham groups. The results of this study suggest that early hematologic and biochemical mean values occurred after acute MI are similar to those of human acute MI. In conclusion, we could observe the alterations and serial values in hematologic and biochemical analysis to the extent of chronic status after acute MI.

Serial values for hematologic and biochemical analysis after myocardial infarction in rats

Lee, Mi-Jin,Tae, Hyun-Jin,Li, Ying-Hua,Yu, Do-Hyeon,Han, In-Ae,Lee, Seok-Won,Ahn, Dong-Choon,Kim, In-Shik,Park, Jin-Ho The Korean Society of Veterinary Service 2008 韓國家畜衛生學會誌 Vol.31 No.2

To diagnose acute myocardial infarction (MI), many cardiac markers have been used in hematologic and biochemical analysis, and many studies have been published for hematologic and biochemical analysis associated with human acute MI. However, after occurrence of acute MI, the serial investigation for values in hematologic and biochemical analysis including chronic MI has rarely been performed. To observe the change of the serial values in hematologic and biochemical analysis, we induced artificial MI. The left main descending artery (LMDA) of the left coronary artery was ligated during the progression (day 1, 3, 5, 7, 14 and 30) of MI. Total 66 Sprague-Dawley rats were divided into the sham group (n=24, thoracotomy without LMDA ligation) and the experimental (MI) group (n=42, with LMDA ligation). And all individual in each group was sacrified at day 1, 3, 5, 7, 14 and 30 for the hematologic and biochemical analysis. In comparison of hematologic analysis between the sham and MI groups, the mean values of red blood cell (RBCs), hemoglobin and hematocrit (HCT) showed a steady increase. In biochemical analysis, the mean values of glucose, cholesterol, total creatine kinase (CK) and isoenzyme MB, and lactate dehydrogenase (LDH) were increased in all MI groups compared with the sham groups. The results of this study suggest that early hematologic and biochemical mean values occurred after acute MI are similar to those of human acute MI. In conclusion, we could observe the alterations and serial values in hematologic and biochemical analysis to the extent of chronic status after acute MI.

Effects of sperm insemination on the final meiotic maturation of mouse oocytes arrested at metaphase I after in vitro maturation

Yoon, Jeong,Juhn, Kyoung-Mi,Yoon, San-Hyun,Ko, Yong,Lim, Jin-Ho The Korean Society for Reproductive Medicine 2017 Clinical and Experimental Reproductive Medicine Vol.44 No.1

Objective: The aims of this study were to investigate whether fertilization could induce the resumption of meiosis in mouse oocytes arrested at metaphase I (MI) after in vitro maturation (IVM), and to investigate the effect of $Ca^{2+}$ chelator treatment at the time of fertilization on the transition from MI to metaphase II (MII). Methods: MII-stage and arrested MI-stage mouse oocytes after IVM were fertilized, and then embryonic development was monitored. Blastocysts from each group were transferred into 2.5 days post-coitum pseudo-pregnant ICR mice. MI oocytes after IVM were treated with a $Ca^{2+}$ chelator to investigate the effect of $Ca^{2+}$ oscillations on their maturation. Results: As insemination time increased, the number of oocytes in the MI group that reached the MII stage also increased. The blastocyst rates and total cell numbers in the MII group were significantly higher than in the MI group. No pregnancy occurred in the MI group, but 10 pregnancies were achieved (10 of 12) in the MII group. The proportion of MI oocytes that matured to MII oocytes after fertilization was significantly higher in the non-treated group than in the $Ca^{2+}$ chelator-treated group. Conclusion: The findings that a higher proportion of MI-arrested oocytes progressed to MII after fertilization and that the MI-to-MII transition was blocked by $Ca^{2+}$ chelator treatments before fertilization indicate that the maturation of MI oocytes to MII oocytes is associated with intracellular $Ca^{2+}$ oscillations driven by fertilization.

미세각막절개 백내장 수술 후 낭내 삽입한 일체형 비구면 인공수정체의 임상 결과

김윤전,전미현,고동아,김재용,김명준,차흥원,Yoon Jeon Kim,Mi Hyun Cheon,Dong Ah Ko,Jae Yong Kim,Myoung Joon Kim,Hung Won Tchah 대한안과학회 2013 대한안과학회지 Vol.54 No.4

Purpose: To evaluate the stability and optical performance of the newly developed single-piece aspheric intraocular lens (IOL) by comparing the clinical outcome of the aspheric IOL with the new optic profile design (HOYA iSert, HOYA iMics) and the aspheric IOL (Akreos MI60), which has been proven effective and safe. Methods: iSert, iMics, and MI60 were inserted into 55 eyes, 60 eyes, and 50 eyes, respectively, after microincision phacoemulsification cataract surgery. Best corrected visual acuity (BCVA), refraction in spherical equivalent, anterior chamber depth (ACD), total higher order aberration (HOA), contrast sensitivity, and surgically induced astigmatism (SIA) were measured and each IOL was evaluated on the functional stability, anterior-posterior stability, centration in the capsular bag, and quality of vision. Results: No statistical differences in preoperative and postoperative BCVA among the 3 IOL groups were observed, however, MI60 showed significant myopic shift postoperatively. Anterior-posterior stability assessed with postoperative change in refractive error and ACD was slightly lower in the MI60 group. In terms of vision quality, while total aberration, total HOA, coma aberration, and contrast sensitivity for the 3 IOLs were not different significantly, spherical aberration of the MI60 group was higher than the other groups at 6 months postoperative. SIA was significantly increased in eyes implanted with iSert than in eyes with iMics or MI60 at 1 month postoperatively, however, the differences were no longer evident after 3 months postoperatively. Conclusions: The new aspheric IOLs, iSert and iMics, showed good stability and visual outcome equal to MI60 at the 6-month postoperative follow-up.

Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

Curcumin과 Protoporphyrin류 화합물의 상호작용에 의한 형광특성 변화

김현정,김미리,최보희,홍정일 서울여자대학교 자연과학연구소 2013 자연과학연구논문집 Vol.25 No.-

Curcumin, a dietary polyphenol from Curcuma longa L., has been reported to show many beneficial bioactivities. Protoporphyrins are widely distributed in various natural sources. They are also intermediates in the endogenous synthesis of heme and commonly form a complex with metal ions. In the present study, we investigated changes in fluorescence properties of curcumin and protoporphyrin IX (PPIX) through their interactions. Fluorescence intensity of curcumin increased concentration-dependently showing a peak emission at 535 nm with an excitation at 440 nm. Curcumin fluorescence was not changed by PPIX; the fluorescence intensity of PPIX significantly decreased in the presence of curcumin. Curcumin fluorescence decreased time-dependently; the fluorescence from PPIX was rather enhanced according to time, which was less pronounced in the presence of curcumin. Curcumin fluorescence in cells decreased markedly, which is slightly enhanced by co-treatment of PPIX. Curcumin fluorescence also decreased by zinc protoporphyrin; the fluorescence from ZnPP was also quenched by curcumin. The present results suggest a possible interaction of metal porphyrins with curcumin, which may influence on their bioactivities.

링컨센터(LCI)의 교사 재교육에서 심미적 교육에 대한 고찰

백미현,이희수 한국문화교육학회 2010 문화예술교육연구 Vol.5 No.3

일반적으로 교사들의 대표적 재교육인 교원연수는 전공과 관련한 이론 보충이나 교수법, 보편적 교양 등이며. 그 형식은 강의식과 토론 위주인 것으로 알려져 있다. 해마다 되풀이 되는 형식과 내용으로는 교사들의 재교육 기대감이나 정신적 만족감을 충족시키기 어렵다. 그로 인해 교육현장에서의 학습전이를 기대하기 힘들다. 본 연구는 기존 교사교육과정의 한계를 극복하는 대안으로서의 문화예술교육 프로그램을 활용한 교사 재교육에 대한 의미와 가치를 제시하고자 미국 뉴욕의 링컨센터의 심미적 교육 입문과정의 참여 경험을 바탕으로 사례를 분석하였다. 링컨센터는 Dewey의 심미적 경험과 탐구이론을 수용, 이를 예술교육 프로그램에 적용한 Maxine Greene의 철학에 기초하여 지속적으로 프로그램을 발전시키고 있다. 연구결과, 예술강사, 교육행정가, 기획자와 일반 교사들을 교육대상으로 적용한 이 심미적 교육의 특징은 수업설계과정과 교육과정의 순서적 특징, 선지식의 배제로 인한 상상력학습, 창작자 위치에서의 작품제작 경험, 탐구과정을 통한 예술작품의 분석, 촉진자와 전문가의 역할, 예술작품 범위의 개방성 등으로 나타났다. 심미적 교육이 교사재교육에 활용될 때 교사의 창의력, 업무처리 능력, 정신적 만족감 등이 향상되어 궁극적으로 교육현장에 긍정적인 파급효과를 가져올 것으로 기대된다. The in-service training, which is one of the most typical re-educational programs teachers, is generally organized with forms of lectures on the supplementary theories which are related with teachers' majors, teaching methods, or general refinement. It is repeated again without changing the forms and contents. Therefore, it is hard to meet the teachers' needs and expectation. In this regard, this study is an attempt to analyze the Introductory Course of Aesthetic Education at Lincoln Center Institute for the Arts in Education for the purpose of proposing the value of re-education utilizing the culture & arts education program. LCI is developing the program based on Maxine Greene's art education philosophy that accepts Dewey's theory of aesthetic experience and inquiry. The aesthetic education programed for art instructors, educrats, architects, and teachers has features as follows; procedural synergy of class design and curriculum development, imaginatory learning excluded prior knowledge, experiencing making art work with artistic point of views, appreciating works through inquiring process, roles of facilitators and experts, and broadening boundaries of defining works of art etc. We expect spin-offs by Aesthetic Education be-all and end-all to learning environment; the program could develop teachers' creativity, performance as well as growing satisfaction when it should be applied to the re-education for themselves.

초등 영재학생과 일반학생의 진로인식, 과학 선호도 및 과학자의 정형화된 이미지 비교

안미정,유미현 韓國英才學會 2012 영재교육연구 Vol.22 No.3

본 연구의 목적은 초등학교 영재학급 학생과 일반학급 학생들의 진로인식과 과학 선호도 및 과학자의 정형화된 이미지를 비교하는 것이다. 본 연구를 위해 초등학교 6학년 영재학생 52명, 일반학생 80명이 연구에 참여하였다. 본 연구의 결과는 다음과 같다. 첫째, 영재학생과 일반학생의 진로인식을 비교한 결과, 영재학생들의 진로인식이 더 높게 나왔으며, 두 집단 간의 유의미한 차이가 나타났다. 이를 통해 전반적으로 일반학생에 비해서 영재학생의 진로인식이 높다는 것을 알 수 있다. 둘째, 영재학생과 일반학급 학생의 과학 선호도는 영재학생이 유의미하게 높은 결과가 나왔다. 하위영역별로 자세히 보면 6개의 모든 하위영역에서 영재학생이 일반학생보다 유의미하게 높았으며, 성별에 따른 비교에서도 영재학생에서만 유의미한 차이가 있었다. 또한 집단과 성별의 차이 간 상호작용 효과는 일반학생에 비하여 영재학생이 과학 학습의 흥미와 학습 지속 실행의지가 높게 나타났다. 셋째, 영재학생과 일반학생의 정형화된 과학자의 이미지 검사를 실시한 결과, 영재학생에 비해 일반학생이 느끼는 과학자의 이미지에서 더 많은 정형화된 모습을 볼 수 있었다. 넷째, 영재학생의 진로인식과 과학 선호도, 진로인식과 과학자의 정형화된 이미지의 상관관계를 살펴본 결과 각각의 변인들 간에 유의미한 상관관계가 나타났다. 다중회귀분석을 통해 진로인식이 과학 선호도에 유의미하게 영향을 준다는 결과를 얻었다. The purpose of this study was to investigate the career awareness, the preference for science and stereotypic image of the scientist between the gifted students and non-gifted students in elementary school. For this study, 52 gifted students and 80 non-gifted students were participated. The results were as follows: First, the career awareness of gifted students was significantly higher than that of non-gifted students. Second, the preference for science of gifted students was significantly higher in all sub-domains than non-gifted students. There was a significant interaction effect between group and gender in the scientific interest and the intention of solving problems. Third, analyzing stereotypic image test for scientist between the gifted students and non-gifted students, it proved that non-gifted students had more stereotypic image comparing with the gifted students. Forth, carrying out the correlation analysis on the career awareness and the preference for science, the career awareness and the stereotypic image of the scientist, it proved that there were significant correlations with each other. It revealed that the career awareness affected the preference for science significantly through multiple regression analysis.

폐암환자의 생존율에 영향을 미치는 예후인자

현미경,이태용 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

This study was performed to know the survival rate and prognostic factors of lung cancer patient who were hospitalized in E university hospital among records in Daejeon cancer registry between 1993 to 2001. There were no differences between man and woman in terms of 1-year and 3-year survival rate. There was no difference between the survival rate of smoker and that of non-smoker. The survival rate of patients having hypertension was significantly different from that of patients not having hypertension. At the time of diagnosis, coughing, dyspnoea and chest pain were the most frequent clinical symptoms of these patients, but there were no differences in 1-year and 3-year survival rate according to the existence of these symptoms. The upper lobe was most frequent primary site of lung cancer. The survival rate of the patients whose primary site was the middle lobe was highest. The survival rate of squamous cell carcinoma was highest. Statistical significance according to the clinical symptoms of a patient demonstrated the symptoms such as coughing, dyspnoea or chest pain were related to the survival rate. Also it demonstrated that the prognostic factors such as age, primary lesion, and histology type affects the survival rate statistically significantly.

인지행동요법과 현실요법이 대학생의 문제해결능력과 역기능적 태도에 미치는 효과

현미열 韓國學校保健學會 2005 韓國學校保健學會誌 Vol.18 No.2

Purpose:This study examined the effects of cognitive-behavioral therapy on problem-solving ability and dysfunctional attitudes in undergraduate students. Method: This study was conducted between September 2004 and May 2005. The subjects consisted of 23 undergraduate students. The experimental group (n=13) participated in a cognitive-behavioral therapy that consist of 8 session over an 8-week period. The control group (n=10) participated in a reality therapy that also consisted of 8 sessions over an 8-week period. Problem-solving ability and dysfunctional attitudes were measured for all subjects before and after therapy. These data were analyzed by unpaired t-test and t-test with the SAS program. Results:Following cognitive-behavioral therapy, the experimental group experienced a significant decrease in score of problem-solving ability and in approach-avoidance style (the subscale in the problem-solving ability). There were no changes in these values after the reality therapy in control group. Conclusions:In light of these results, cognitive-behavioral therapy has a positive effect on problem-solving ability in undergraduate students.