Transcriptional activation of hypoxia-inducible factor-1α by HDAC4 and HDAC5 involves differential recruitment of p300 and FIH-1

Seo, Hee-Won,Kim, Eun-Jin,Na, Hyelin,Lee, Mi-Ock Elsevier 2009 FEBS letters Vol.583 No.1

<P><B>Abstract</B></P><P>The interplay between hypoxia-inducible factor-1α (HIF-1α) and histone deacetylase (HDACs) have been well studied; however, the mechanism of cross-talk is unclear. Here, we investigated the roles of HDAC4 and HDAC5 in the regulation of HIF-1α function and its associated mechanisms. HDAC4 and HDAC5 enhanced transactivation by HIF-1α without stabilizing HIF-1α. HDAC4 and HDAC5 physically associated with HIF-1α through the inhibitory domain (ID) that is the binding site for factor inhibiting HIF-1 (FIH-1). In the presence of these HDACs, binding of HIF-1α to FIH-1 decreased, whereas binding to p300 increased. These results indicate that HDAC4 and HDAC5 increase the transactivation function of HIF-1α by promoting dissociation of HIF-1α from FIH-1 and association with p300.</P><P><B>Structured summary:</B></P><P>MINT-6802187:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>FIH1</I> (uniprotkb:Q9NWT6) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802058:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>HDAC4</I> (uniprotkb:P56524) by <I>pull down</I> (MI:0096)</P><P>MINT-6802021:</P><P><I>HIF1 alpha</I> (uniprotkb:Q61221) <I>physically interacts</I> (MI:0218) with <I>HDAC4</I> (uniprotkb:P56524) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802036:</P><P><I>HIF1 alpha</I> (uniprotkb:Q61221) <I>physically interacts</I> (MI:0218) with <I>HDAC5</I> (uniprotkb:Q9UQL6) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802102:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>HDAC5</I> (uniprotkb:Q9UQL6) by <I>pull down</I> (MI:0096)</P><P>MINT-6802121, MINT-6802156:</P><P><I>P300</I> (uniprotkb:Q09472) <I>physically interacts</I> (MI:0218) with <I>HIF1 alpha</I> (uniprotkb:Q16665) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P>

Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

혈액투석실 간호사의 비판적 사고성향과 임상의사결정능력이 환자안전역량에 미치는 영향

서미란(Mi-ran Seo),정경희(Kyung-Hee Chung) 인문사회과학기술융합학회 2018 예술인문사회융합멀티미디어논문지 Vol.8 No.8

This descriptive research is to provide preliminary data for the development of a nursing service program that ensures the safety of hemodialysis patients by identifying the effects of nurses’ critical thinking disposition and clinical decision making ability on the patient safety competence. The research participants were recruited through convenience sampling. The survey was conducted on 202 nurses who had worked for three months or longer in a hemodialysis unit. Critical thinking disposition was measured using the instrument to measure critical thinking disposition developed by Yun Jin (2004), and clinical decision making ability was measured using the Korean version of the Clinical Decision Making in Nursing Scale (Jenkins, 1985), which was translated and revised by Baek Mi-kyung (2005). The instrument to measure patient safety competence in this study is the one developed by Lee Nam-ju (2012) and then revised and supplemented by Jang Hae-na (2012) for clinical nurses. In summary, Consequently, education programs tailored for nurses according to their years of experience and position need to be developed and implemented. In addition, policies related to patient safety accidents should be prepared and continuous education should be provided for nurses working at a hemodialysis unit. 본 연구는 서술적 조사연구로 혈액투석실에 근무하는 간호사의 비판적 사고성향과 임상의사결정능력이 환자안전에 미치는 영향을 확인하여 혈액투석환자의 안전간호를 위한 서비스 프로그램 개발의 기초자료를 제공하기 위해 수행되었다. 연구대상은 혈액투석실 근무경력 3개월 이상의 간호사 202명이며, 구조화된 설문지를 이용하여 자료를 수집하였다. 연구도구는 윤진(2004)이 개발한 비판적 사고성향 측정도구, Jenkins(1985)가 개발한 The Clinical Decision Making in Nursing Scale을 백미경이(2005)이 수정한 임상의사결정능력 측정도구, 이남주(2012)가 개발한 환자안전역량 측정도구를 장해나(2013)가 수정한 도구를 사용하였다. 연구결과 혈액투석환자의 안전간호 실현을 위해 간호사의 비판적 사고성향과 임상의사결정 능력이 주요한 영향요인임을 확인하였으므로 직급별 맞춤형 교육프로그램을 개발하여 적용하고, 환자안전을 위한 정책을 마련하여 지속적으로 환자, 보호자 및 간호사에 대한 교육이 필요할 것으로 사료되었다.

두경부 마사지가 중환자실 환자의 수면과 불안에 미치는 효과

김미용,전선영,송윤희,최은진,김재희,김미성,주명순,김남선 병원간호사회 2006 임상간호연구 Vol.11 No.2

Purpose: This study was to apply head and neck massage to patients in intensive care unit and to inventigate the effect of that massage on sleep and state anxiety. Method: The subjects in this study were 27 patients who were admitted in medical intensive care unit. The study was performed from June thru September of 2005 on the One-group pretest-posttest design and the sleep, state anxiety of the subjects were measured before and after head and neck massage. For data analysis, paired t-test and Pearson correlation coefficient were utilized. Result: The first hypothesis that the subjects might have a better sleep after being exposed to head and neck massage was accepted. The second hypothesis that the subjects might feel less state anxiety afrer being exposed to head and neck massage was accepted. The third hypothesis that the sleep of the ICU patients maight be correlated to their anxiety was accepted, as there appeared correlation between their sleep and anxiety. Conclusion: Head and neck massage is identified as one of independent nursing interwentions to improve the sleep of ICU patients and ease their anxiety, and it is necessary to apply it to clinical practices.

Bacillus species related to food poisoning

Oh, Mi-Hwa,Kim, Gun-Hee 식물자원연구소 덕성여자대학교 2006 植物資源硏究誌 Vol.5 No.-

The major concern in the food industry is the outgrowth of pathogenic Bacillus species which have the potential to cause a food poisoning incident. Bacillus cereus is the most prevalent pathogenic Bacillus species found in food and other industrial processes. However, other species in the genus Bacillus, including B. subtilis, B. licheniformis, B. brevis, B. pumilus and B. thuringiensis have been implicated as potential agents of food poisoning.

도라지가 난소를 절제한 흰쥐의 혈 중 지질 함량 변화에 미치는 영향

김미향,김원희,박미라,한희선,배송자 신라대학교 자연과학연구소 2003 自然科學論文集 Vol.11 No.-

This study was designed to observe the effects of the feeding Platycodon grandiflorum A.DC(PG) extract on the improvement of the lipids on serum in ovariectomized rats. To investigate the effects of ethanol extract, Sprague-Dawley female rats were randomly assigned to groups as follows: Sham, OVX-control and ovariectomized rats supplemented with PG at 50㎎/㎏ bw/day. Body weight gain was not significantly different in groups. The levels of total cholestcrol was higher in ovaiectornized control rats than Sham-operated rats, but supplementation of PG ethanol extracts at 50mg/kg bw/day decreased the level of cholesterol in surum. These results might be expected that ethanol extract of PG is believed to be possible protective effects for the fatty serum increasing serum lipid.

신규간호사 배치후 교육시 프리셉터쉽 적용 사례연구

김창희,김혜숙,조혜성,이순옥,함형미,박미미 성인간호학회 2000 성인간호학회지 Vol.12 No.4

The purpose of this study was to examine the effect of preceptorship on On the Job Training of new nurses. The research design was a retrospective survey and one group pre-rost test design. The subjects were 49 entry nurses and 49 preceptors The data were collected from Feburary 1, 1998 to December 1, 1998. One questionnair applied in this study consisted of general characteristics, the responses of new entry nurses and their preceptors and their qualities. The other questionnair consisted of job satisfaction of new entry nurses and Preceptors and their competencies. The data were analyzed for frequencies, mean score, standard deviation, t-test, and ANOVA. The results of the study were as follows : 1) Preceptees's responses were that the nurses' affiliation of unit. cooperation of other nurses, and competencies of nursing skills improved after OJT applied preceptorship. 2) Preceptors's answers were that preceptorship had a positive effect on their continuing education. 3) While preceptees's job satisfaction scores were high in interaction, administration, and organization, they were low in autonomy and pay. 4) Pre-competency scores were the same as post-competency scores. 5) After preceptorship, preceptors responded that their nursing skill & communication competency increased, but their nursing administration competency decreased. 6) Finally, preceptorship's other effects were as follows: ① Checklists for preceptee education at the nursing unit were developed separately. ② It was proved that 'preceptor interview note' was effective on manage preceptorship. ③ We obtained duty orierlap periods, medical-surgical units-1 month. 5 ICU-2 months. operaction room-3 months ④ We prepared a preceptor program based on the study results.

심한 낙타혹(Hump)의 형성을 동반한 제 2 형 막증식성 사구체신염(Dense Deposit Disease) 1예

오희정,유민아,이영숙,김미연,김승정,강덕희,윤견일,최규복,성순희 이화여자대학교 의과대학 2004 EMJ (Ewha medical journal) Vol.27 No.1

Dense deposit disease (DDD), known as type Ⅱ MPGN, is an uncommon form of glomerulonephritis. It is an acquired primary glomerular disease, characterised by electron microscopic evidence of a continous, dense membrane deposition replacing the lamina densa. There is associated alternative pathway complement activation and a C3 nephritic factor maybe present. Patients with dense deposit disease tend to be young at clincal onset and more commonly have persistent nephrotic syndrome, gross hematuria, and acute nephritis episode and persistent depression of the serum complement level. The prognosis of dense deposit disease is worse than that of typeⅠMPGN. We experienced a patient with dense deposit disease that presenting persistent nephrotic syndrome, gross hematuria and having subepithelial hump. It is a first report of dense deposit disease having subepithelial hump in Korea, therefore we report this case with the review of relevant literatures.

자발성 뇌내출혈 환자의 비위관 제거시기에 관한 연구

김명희,김영희,김영미,주영희,이윤미,정은혜 성인간호학회 2001 성인간호학회지 Vol.13 No.1

Dysphagia is common and serious problem in intracerebral hemorrhage patients. Nasogastric-tube incubation is an important method for dysphagic patients who have an intracerebral hemorrhage. But many discomforts develope in patients with a nasogastric-tube. Therefore, it is necessary to decide when to remove the tube and as early as possible. The purpose of this study is to decide the applying time of training program to facilitate deglution for dysphagic patients who have intracerebral hemorrhage. Among the 343 patients with intracerebral hemorrhage who had been admitted to P-university hospital from April, 1994 to December, 1998, the medical record of the 110 patients with nasogastric tube were reviewed retrospectively. Results from the study were as follows : 1.Nasogastric tube insert duration of improved patients was a mean of 23.2 days 2.When a L-tube was inserted and removed, the L.O.C. of improved patients was 2.6±0.8(in the midst of stupor or a semicoma). 1.9±0.5(close to drowsy), respectively This was signifcantly different(p=0.000). 3.When a L-tube was inserted and removed, GCS of improved patients was 9.3±2.9. 12.1±2.2 respectively This was significantly different (p=0.000). 4.The duration of nasogastric tube insertion was the longest when in a stupor(L.O.C), 6 ∼8 points(GCS).

프로토콜 분석을 통한 창의적 디자인 전략으로서의 은유와 유추

최한희(Choi, Han_Hee),주서령(Ju, Seo Ryeung),김미정(Kim, Mi Jeong) 한국실내디자인학회 2013 한국실내디자인학회논문집 Vol.22 No.1

The aim of this research is to explore the potential of analogy and metaphor as design strategies for supporting the creative design thinking. For the empirical research on analogical and metaphoric process in designing, we conducted design experiments that consist of three different conditions of given items as stimulation for design inspiration: baseline, surrealist paintings, and housing design collections. The results were analyzed using a protocol analysis in order to obtain more systmatic interpretation of the design processes and strategies. As a result, it was noted that students are more apt to read visual information rather than semantic information in the given items. Instead of the representation of their senses or feelings from the paintings, they visualized the analogical images of the paintings for the design representation. However, analogical and metaphoric thinking derived from the given items seem change a designer’s perspective, thus bring a novel interpretation on design problems, and eventually more creative and meaningful design ideas. An extended research using one-semester training and observation of the design studio process is introduced as a follow-up study for this paper. This research will investigate the long-term effect of the analogy and metaphor on the design thinking.