Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

Hepatic Cirrhosis Occurring in a Young Woodchuck(Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus(WHV)

Kyu-shik Jeong,정원일,Jae-yong Chung,Mi-young An,Chae-yong Jung,Gyoung-jae Lee,Jong-soo Kang,Byeong-cheol Kang,Young-heun Jee,Bruce H Williams,Young-oh Kwon,Da-hee Jeong 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Cirrhosis Occurring in a Young Woodchuck (Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus (WHV)Da-hee Jeong, Won-il Jeong, Jae-yong Chung, Mi-young An, Chae-yong Jung, Gyoung-jae Lee1, Jong-soo Kang1, Byeong-cheol Kang2, Young-heun Jee3, Bruce H Williams4, Young-oh Kwon5 and Kyu-shik Jeong*College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea1Shinwon Scientific Co., LTD, Research Institute, Suwon, Korea2Clinical Research Institute, Seoul National University, Seoul 110-744, Korea3College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea

여성 연소노인의 지각연령과 사회무용지도자 실제에 따른 지도효율성

정문미(Jung Moon Mi),원영신(Won Young Shin) 글로벌시니어건강증진개발원 2011 글로벌시니어건강증진개발원 논문집 Vol.1 No.1

본 연구는 여성 연소노인의 사회 인구학적 특성에 따른 사회무용 지도자 실제, 사회무용 지도자 실제에 따른 지도 효율성의 차이, 그리고 지각연령이 지도 효율성에 미치는 영향을 규명하는 데 목적이 있다. 이와 같은 연구목적을 달성하기 위하여 서울지역 소재 노인교육기관(노인교실, 노인대학, 노인학교, 노인복지회관, 사회복지회관, 대학부설 평생교육원 및 사회교육원, 종교기관 등)에서 사회무용 프로그램에 참여한 경험이 있는 여성 연소노인을 모집단으로 설정한 다음, 유의표본표집법을 이용하여 450명의 연구대상을 표집하였으며, 불성실하게 응답한 자료를 제외하고 총 428명의 자료를 최종분석에 사용하였다. 자료처리는 SPSS Windows 15.0 version을 이용하여 빈도분석, 탐색적 요인분석, 신뢰도분석, 카이검정, 상관관계, 다중 회귀분석, 공분산분석을 실시하였으며, 다음과 같은 결론을 도출하였다. 첫째, 사회 인구학적 특성에 따른 사회무용 지도자 실제는 참여기관 유형에 있어서만 사회무용 지도자 실제 하위특성 모두에 한 차이가 나타났다. 또한 결혼유지 상태는 지도자의 성별, 연령을 제외, 교육수준과 경제수준은 성별을 제외, 직업상태(현재)는 성별과 전공경력을 제외한 모든 사회무용 지도자 실제 하위특성에 유의한 차이가 나타났다. 역연령은 지도자의 전공경력에서만 유의한 차이가 나타났다. 둘째, 지각연령이 지도 효율성에 미치는 영향을 분석한 결과, 지각연령의 하위요소 중, 보이는 연령이 지도 효율성의 하위 요소 중, 인지된 지도만족(B=-.106)에 부적(-), 활동 연령이 인지된 기능향상(B=.109)과 인지된 지도만족(B=.178)에 있어 모두 정적(+) 영향을 미치는 것으로 나타났다. 셋째, 사회 인구학적 특성과 지각연령이 일정한(통제된) 경우, 실제 사회무용 지도자의 하위특성인 성별, 연령, 교육수준, 전공경력 모두에 따라 지도 효율성의 하위요인 모두에 유의한 차이가 있었다. The purpose of this study is to find the between the young-old woman the demographic characteristics and practice of social dance instructor, the difference of between practice of social dance instructor and coaching effectiveness, and tne effect of between the self-perceived age and oaching effectiveness. The subjects in this study were 450 women who were selected by purposive sampling from a population of the young-old woman who had ever participated in social dance programs in senior education institutions senior class, senior college, senior school, Senior welfare center, social welfare center, university, school of lifelong education, religion community) in Seoul. After a survey was conducted, the answer sheets from 428 respondents were analyzed except some incomplete ones. As for data handling, a SPSS Windows Ver. 15.0 program was employed, and a frequency analysis, exploratory factor analysis, reliability analysis, pearson chi-square, binary-logistic regression, ANCOVA and were utilized. The findings of the study were as follows: First, practice of the coaches according to social population characteristics shows the significant difference for all practical lower facts of the coaches only in types of participation institute. Also, marriage status without the coaches’ gender and age, educational and economic level without gender, and current job status without the gender and career show the significant difference in practical lower facts of all the coaches. Chronological age shows the significant difference only in the career of the coaches. Second, the analysis of the influence of elf-perceived age on the coaching effectiveness shows all recognized coaching satisfaction(B=-.106) of (-), function improvement(B=.109) with the recognition of activity age and recognized coaching satisfaction(B=.178) were influenced in (+) way. Third, in case of social population characteristics and self-perceived age are controled, all lower factors of coaching effectiveness shows significant difference according to the actual coaches’ gender, age, educational level and career.

여성 연소노인의 지각연령에 따른 사회무용 지도자 선호에 대한 연구

정문미(Jung, Moon Mi),원영신(Won, Young Shin),고대선(Ko, Dae Sun) 한국노년학회 2010 한국노년학 Vol.30 No.2

본 연구는 여성 연소노인의 사회 인구학적 특성에 따른 지각연령의 차이를 알아보고 지각연령이 사회무용 지도자 선호에 미치는 영향을 규명하는 데 목적이 있다. 이와 같은 연구목적을 달성하기 위하여 서울지역 소재 노인교육기관(노인교실, 노인대학, 노인학교, 노인복지회관, 사회복지회관, 대학부설 평생교육원 및 사회교육원, 종교기관 등)에서 사회무용 프로그램에 참여한 경험이 있는 여성 연소노인을 모집단으로 설정한 다음, 유의표본표집법을 이용하여 450명의 연구대상을 표집하였으며, 불성실하게 응답한 자료를 제외하고 총 428명의 자료를 최종분석에 사용하였다. 자료처리는 SPSS Windows 15.0 version을 이용하여 빈도분석, 탐색적 요인분석, 신뢰도분석, 카이검정, 독립표본 t-test, 일원분산분석, 이항 로지스틱 회귀분석을 실시하였으며, 다음과 같은 결론을 도출하였다. 첫째, 사회 인구학적 특성에 따른 지각연령은 역연령, 교육수준에 있어서 지각연령 하위요인(보이는 연령, 느끼는 연령, 관심사 연령, 활동 연령) 모두에 유의한 차이가 나타났다. 또한 결혼유지 상태는 관심사 연령과 느끼는 연령, 경제수준은 보이는 연령, 관심사 연령, 느끼는 연령에 있어서만 유의한 차이가 나타났다. 둘째, 사회 인구학적 특성에 따른 사회무용 지도자 선호는 교육수준에 있어서 사회무용 지도자 선호 하위특성(성별, 연령, 교육수준, 전공경력) 모두에 유의한 차이가 나타났다. 또한 역연령은 지도자의 전공경력을 제외, 경제수준은 지도자의 성별을 제외한 모든 사회무용 지도자 선호 하위특성에 유의한 차이가 나타났으며, 결혼유지 상태는 지도자의 교육수준에 있어서만 유의한 차이가 나타났다. 셋째, 지각연령이 사회무용 지도자 선호에 미치는 영향을 분석한 결과, 지각연령의 하위 요소 중 보이는 연령만이, 사회무용 지도자 선호하위특성 중 전공경력을 제외한 성별(B=.458), 연령(B=.483), 교육수준(B=.76) 모두에 유의한 정적(+) 영향을 미치는 것으로 나타났다. The purpose of this study was to examine the demographic characteristics of the female young-old to their self-perceived age and the impact of their self-perceived age on preference for a social dance instructor. The subjects in this study were 450 women who were selected by purposive sampling from a population of the female young-old who had ever participated in social dance programs in senior education institutions senior class, senior college, senior shool, Senior welfare center, social welfare center, university, school of lifelong education, religion community) in Seoul. After a survey was conducted, the answer sheets from 428 respondents were analyzed except some incomplete ones. As for data handling, a SPSS Windows Ver. 15.0 program was employed, and a frequency analysis, exploratory factor analysis, reliability analysis, pearson chi-square, independent-samples t-test, one-way ANOVA and binary-logistic regression were utilized. The findings of the study were as follows: First, self-perceived age by social population characteristics shows significant difference in all lower factors of self-perceived age(Look age, Feel age, Interest age, Do age) as chronological age, education level. Also, the marriage status shows significant difference only in interest age and feel age, the economic level shows only in look age, interest age and feel age. Second, the social dance instructor preference by social population characteristics shows significant difference in the all lower facts of social dance coaches preference(gender, age, education level, instructor career) up to educational level. Also, chronological age without the instructor career, economic level without instructor gender show the significant difference in the lower facts of all social dance instructor preference. Also, marriage status shows the significant difference in the educational level of the instructor. Third, the analysis of the influence of self-perceived age on the instructor preference shows all gender(B=.458), age(B=.483) and educational lever(B=.76) without career were influenced in significant (+) way.

한국비서자격시험의 개선점과 비교연구

남자현,이미정,이미영,이아영,정해금,최유정,이미숙,전인선,정혜림 이화여자대학교 법정대학 비서학과 1997 비서학연구 Vol.- No.17

악성 섬유성 조직구종 1예

정미정,정우권,박영립,황규왕,김영근 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.1

Malignant fibrous histiocytoma is the most common soft tissue sarcoma in adult life and shows a mixed proliferation of both fibroblastic and histiocytic cells. Because of its highly variable morphologic pattern, this tumor has often been confused with other sarcomas such as pleomorphic rhabdomyosarcoma and liposarcoma. We report a case of malignant fibrous histiocytoma occurring in a 22-year-old male who presented with a adult's fist-sized mass with several brown colored nodules on the right buttock of 3 years' duration. Histologic findings shows spindle cells arranged in a storiform pattern characteristic of the typical pleomorphic malignant fibrous histiocytoma.

미치광이풀 모상근 배양에서 Tropane Alkaloid 생산성 증진을 위한 최적 생물학적 엘리시터 선발

정희영,강승미,강영민,김용덕,양재경,정영관,최명석 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Scopolamine and hyoscyamine which belong to tropane alkaloids are the pharmaceutically valuable anticholinergic drugs. In order to increase the productivities, the effects of elicitation were investigated during hairy root cultures of Scopolia parviflora. Biotic elicitors originated from 3 fungi and 1 yeast were prepared as homogenate and supernatant and added to 3-week-old cultures. Both of homogenate and supernatant of Candida albicans elicitors increased the scopolamine production. The production of hyoscyamine was enhanced by homogenate of Fusarium solani and supematant of C. albicans. Most of the other fungal elicitors were also improved on the tropane alkaloid production compared to non-treatment. Among the elicitors tested, C. albicans was proved the optimal biotic elicitor on tropane alkaloids production. These results will be served mass production of tropane alkaloids by large-scale production.

동서양의 식용꽃에 대한 고찰 : 조리방법을 중심으로

김영순,이정희,오순덕,정은미,최지영,유은순,장정미,정진선,하상민 高麗大學校 倂設 保健大學 保健科學硏究所 2001 保健科學論集 Vol.27 No.2

Edible flower means a flower that is good to eat is subject to either cooking or appetizing. In general, people eat edible flower as raw so that the loss of vitamin and inorganic are minimal. Edible flower is usually utilized as salad material or aromatic, decoration for jelly or cake and solid stuff in soup. Thus, decorating food by edible flower improves flavor, fragrance and appetite. Edible flower serves various purposes both east and west. In Korea, Edible flower is mainly subject to soup, cooked potherbs, frying, tea stuff, brewing and etc, whereas in Japan, its various usage covers cooked potherbs, pickle, sliced raw fish, frying, salad, jelly and decoration. On the other hand, Edible flower is used as numerous salad and tea stuff, condiment, roasting, meat, sweets and desserts in Western Hemisphere.

퍼머넌트와 염색 연결시술시 정향트리트먼트 적용시점에 따른 모발보호효과

박미나ㆍ정지영ㆍ고경숙(Mi na ParkㆍJee Young JungㆍKyoung sook Ko) 한국인체미용예술학회 2016 한국인체미용예술학회지 Vol.17 No.1

This study attempted to determine the effects of hair protection by investigating the characteristics and possibilities of hair cosmetics using the hot water extracts of Syzygium aromaticum and then hair-protection effects were confirmed through pre-treatment and post-treatment at consecutive chemical treatments. At the consecutive chemical treatments, the general treatment was divided into pretreatment(N1) and general treatment post-process(N2). Then, the Syzygium aromaticum-based treatment was divided into pre-treatment(J1) and post processing(J2). According to the analysis, tensile strength was the highest in the Syzygium aromaticum extract treatment group. At consecutive treatments, ‘J2’ which processed treatment for Syzygium aromaticum, was the highest. In terms of hair thickness, the Syzygium aromaticum group was greater than the general treatment group. When measured to determine the morphological characteristics of ‘Hair’ at 20,000 magnifications using SEM, the Syzygium aromaticum extract post-treatment group (J2) revealed the highest value (373.98±10.83㎚). Regarding hair composition under the EDS, Syzygium aromaticum extract post-treatment group was the highest with 07.77% interms of sulfur content. The results confirmed that Syzygium aromaticum extract treatment is extremely effective in easing hair damage which is caused by consecutive dyeing after permanent. Therefore, the Syzygium aromaticum extract treatment would be effective in preventing hair damage and keeping hair healthy and glossy.

연구논문 : 대학생들의 인터넷 중독에 영향을 미치는 요인에 대한 연구

권미영 ( Mi Young Kwon ),황지영 ( Ji Young Hwang ),정혜윤 ( Hye Yoon Jung ),송시형 ( Shi Hyeong Song ) 극동대학교 사회복지연구소 2013 글로벌사회복지연구 Vol.3 No.2

본 논문의 목적은 대학생의 인터넷 중독에 영향을 주는 요인에 대한 분석을 통해 대학생의 정신건강을 위한 공적프로그램 도입에 대한 함의를 제공하는 것이다. 분석자료는 을지대학교 강남 을지 병원 중독연구소에서 대학생 453명을 대상으로한 설문지이다. 설문조사에 사용된 설문지는 인터넷 중독을 측정하는 인터넷 중독 척도(Young척도), 불안을 측정하는 불안척도(STAI)와 우울을 측정하는 Beck의 우울척도(BDI)로 구성되었다. 분석결과 첫째, 연령 및 학년은 인터넷 중독에 유의미한 영향을 미쳤다. 둘째, 나이가 어릴수록, 학년이 낮을수록 불안과 우울에 빠지기 쉬웠다. 셋째, 무엇보다 불안이 대학생의 인터넷 중독에 가장 큰 영향을 미치는 요인이었다. The purpose of this research to suggest implications on introduction of public-program for college students` mental health through analysing the factors which impact on internet addiction of college students. The analysing data is questionnaire intended to 453 college students in Kang- Nam Eulji hospital addiction institute. The questionnaire consisted of internet addiction scale (Young-scale), anxiety scale (STAI) and depression scale (BDI). This study reveals three main findings. Firstly, age and grade have an effect on internet addiction. Second, the younger, the lower grade, they are likely to be depressed and anxiety. Third, most of all, the anxiety has the most effect on internet addiction of college students.