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Menno Hoekstra,Zhengzheng Zhang,Peter W. Lindenburg,Miranda Van Eck 한국지질동맥경화학회 2024 지질·동맥경화학회지 Vol.13 No.1
ObjectiveScavenger receptor class B type I (SR-BI) is primarily known for its role in the selective uptake of cholesteryl esters (CEs) from high-density lipoproteins (HDLs). Here we investigated whether SR-BI deficiency is associated with other potentially relevant changes in the plasma lipidome than the established effect of HDL-cholesterol elevation. MethodsTargeted ultra-high-performance liquid chromatography-tandem mass spectrometry was utilized to measure lipid species in plasma from female wild-type and SR-BI knockout mice. ResultsSR-BI deficiency was associated with a reduction in the average CE fatty acid length (−2%; p<0.001) and degree of CE fatty acid unsaturation (−18%; p<0.001) due to a relative shift from longer, polyunsaturated CE species CE (20:4), CE (20:5), and CE (22:6) towards the mono-unsaturated CE (18:1) species. Sphingomyelin (SM) levels were 64% higher (p<0.001) in SR-BI knockout mice without a parallel change in (lyso)phosphatidylcholine (LPC) concentrations, resulting in an increase in the SM/LPC ratio from 0.102±0.005 to 0.163±0.003 (p<0.001). In addition, lower LPC lengths (−5%; p<0.05) and fatty acid unsaturation degrees (−20%; p<0.01) were detected in SR-BI knockout mice. Furthermore, SR-BI deficiency was associated with a 4.7-fold increase (p<0.001) in total plasma ceramide (Cer) levels, with a marked >9-fold rise (p<0.001) in Cer (d18:1/24:1) concentrations. ConclusionWe have shown that SR-BI deficiency in mice not only impacts the CE concentrations, length, and saturation index within the plasma compartment, but is also associated with plasma accumulation of several Cer and SM species that may contribute to the development of specific hematological and metabolic (disease) phenotypes previously detected in SR-BI knockout mice.
Nico T. Mutters,Caspar J. Hodiamont,Menno D. de Jong,Hendri P. J. Overmeijer,Mandy van den Boogaard,Caroline E.Visser 대한진단검사의학회 2014 Annals of Laboratory Medicine Vol.34 No.2
Background: Microbiological laboratories seek technologically innovative solutions to cope with large numbers of samples and limited personnel and financial resources. One plat- form that has recently become available is the Kiestra Total Laboratory Automation (TLA) system (BD Kiestra B.V., the Netherlands). This fully automated sample processing sys- tem, equipped with digital imaging technology, allows superior detection of microbial growth. Combining this approach with matrix-assisted laser desorption ionization time-of- flight mass spectrometry (MS) (Bruker Daltonik, Germany) is expected to enable more rapid identification of pathogens. Methods: Early growth detection by digital imaging using Kiestra TLA combined with MS was compared to conventional methods (CM) of detection. Accuracy and time taken for microbial identification were evaluated for the two methods in 219 clinical blood culture isolates. The possible clinical impact of earlier microbial identification was assessed ac- cording to antibiotic treatment prescription. Results: Pathogen identification using Kiestra TLA combined with MS resulted in a 30.6 hr time gain per isolate compared to CM. Pathogens were successfully identified in 98.4% (249/253) of all tested isolates. Early microbial identification without susceptibility testing led to an adjustment of antibiotic regimen in 12% (24/200) of patients. Conclusions: The requisite 24 hr incubation time for microbial pathogens to reach suffi- cient growth for susceptibility testing and identification would be shortened by the imple- mentation of Kiestra TLA in combination with MS, compared to the use of CM. Not only can this method optimize workflow and reduce costs, but it can allow potentially life-saving switches in antibiotic regimen to be initiated sooner.
Jeong Hyun Lee(이정현),Ep Heuvelink,Menno J. Bakker 한국원예학회 2009 원예과학기술지 Vol.27 No.3
광수준에 따른 재식밀도가 연중 스프레이 절화국의 생육패턴과 품질에 미치는 상호작용 효과를 구명 하고, 기존의 경험적인 국화 생장 및 품질 예측 모델의 검증을 하고자 본 연구를 수행하였다. 절화국(Chrysanthemum morifolium) ‘Reagan Improved’를 재식밀도 16, 32, 64와 80 plants m?² 와 광수준 100%, 66%와 43%를 조합하여 여름철에 네덜란드에 있는 독립된 유리온실3개 구역에서 실험을 수행하였다. 식물의 생체중과 건물중, 화수와 줄기의 강도 및 초장은 광수준과 재식밀도간의 상호작용 효과가 유의하였으며, 생육량과 줄기의 품질은 광수준이 증가하고 식물체당 차지하는 면적 증가할수록 그 차이가 컸고, 광수준이 낮아 질수록 재식밀도간의 생육특성의 차이가 작았다. 재식밀도가 높은 64-80 plant m?²에서는 초장의 차이가 나타나지 않았지만, 광수준 43%에서는 초장인 64 plants m?²까지 증가하다가 80 plants m?²에서 감소하는 quadratic 반응을 나타내었다. 단위면적당 건물생산량과 엽면적지수는 광수순과 재식밀도에 따라 증가하였고, 광이용효율(LUE)은 가장 낮은 광수준에서 가장 높게 나타났으며, 재식밀도 16-80 plants/m2 범위에서는 quadratic 반응을 보였다. 절화의 외형적인 품질인 초장 대비 스프레이길이 비율(초장대비 첫 개화지 마디부터 생상점까지의 길이의 비)은 엽면적 지수와 부의 상관을 나타내었다. 경험적 모델식 들은 낮은 재식밀도 범위에서 생체중과 꽃의 개수를 과대평가되었다. 경험적인 단순모델은 연구결과를 요약할 수 있는 도구로 사용할 수 있을 것으로 사료된다. 연중 균일한 품질(줄기당 개화수 및 줄기강도, 개체당 줄기의 형태 등)은 광량과 재식밀도간의 상호작용효과가 매우 높기 때문에 스케줄에 의한 재식밀도의 결정보다는 외부광량에 따른 적정 재식밀도를 고려가 매우 중요할 것으로 생각된다. Aims of this study are to analyze growth pattern and development of cut chrysanthemum and test simple regression models in relation to light level and plant density. Cut chrysanthemum (Chrysanthemum morifolium), cv. Reagan Improved, was grown at four different plant densities of 16, 32, 64 or 80 plants m?² combined with three different light levels of 100%, 66% or 43% of light in three greenhouse compartments in summer at Wageningen University in the Netherlands. Plant fresh and dry mass, stem strength, number of flowers and stem length had shown interactive effects between light level and plant density. All these parameters increased with increased light level and with increased plant density; however, the decrease as a result of light reduction was much larger at low plant densities except for stem length. Stem length increased with increased plant density, following a saturation-type of response, except for the lowest light level, where an optimum-response was observed, resulting in a reduced stem length at the highest density. Dry mass production per m2 greenhouse area and final leaf area index increased with increased light level and with increased plant density. Light use efficiency (LUE, unit dry mass per unit of cumulative intercepted PAR) was 48% higher at lower light levels than 100%. LUE also showed a quadratic response to plant density in range of 16 and 80 plant m?². Using an existing empirical linear model for plant, fresh mass and number of flowers for cut chrysanthemum showed overestimation in plant fresh mass and under estimation for number of flowers at the lowest plant density in all light level in this study. Plant shape, calculated as a ratio between stem length and a length between a node with first lateral stem to top point of plant, linearly decreased with increased LAI. Plant qualities hence strongly varied depending on crop canopy like leaf area index. Although the simple regression models are good tools for summary of experiment, it does not fit at lower plant density. To produce a constant product quality throughout the year, optimal number of plant m?² with amount of available light should be considered through the growing period.