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Tarekegn, Getinet Mekuriaw,Ji, Xiao-yang,Bai, Xue,Liu, Bin,Zhang, Wenguang,Birungi, Josephine,Djikeng, Appolinaire,Tesfaye, Kassahun Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.9
Objective: This study was carried out to assess the haplotype diversity and population dynamics in cattle populations of Ethiopia. Methods: We sequenced the complete mitochondrial cytochrome b gene of 76 animals from five indigenous and one Holstein Friesian${\times}$Barka cross bred cattle populations. Results: In the sequence analysis, 18 haplotypes were generated from 18 segregating sites and the average haplotype and nucleotide diversities were $0.7540{\pm}0.043$ and $0.0010{\pm}0.000$, respectively. The population differentiation analysis shows a weak population structure (4.55%) among the populations studied. Majority of the variation (95.45%) is observed by within populations. The overall average pair-wise distance ($F_{ST}$) was 0.049539 with the highest ($F_{ST}=0.1245$) and the lowest ($F_{ST}=0.011$) $F_{ST}$ distances observed between Boran and Abigar, and Sheko and Abigar from the indigenous cattle, respectively. The phylogenetic network analysis revealed that all the haplotypes detected clustered together with the Bos taurus cattle and converged to a haplogroup. No haplotype in Ethiopian cattle was observed clustered with the reference Bos indicus group. The mismatch distribution analysis indicates a single population expansion event among the cattle populations. Conclusion: Overall, high haplotype variability was observed among Ethiopian cattle populations and they share a common ancestor with Bos taurus.
Laser methane detector-based quantification of methane emissions from indoor-fed Fogera dairy cows
Kobayashi, Nobuyuki,Hou, Fujiang,Tsunekawa, Atsushi,Yan, Tianhai,Tegegne, Firew,Tassew, Asaminew,Mekuriaw, Yeshambel,Mekuriaw, Shigdaf,Hunegnaw, Beyadglign,Mekonnen, Wondimeneh,Ichinohe, Toshiyoshi Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.8
Objective: Portable laser methane detectors (LMDs) may be an economical means of estimating CH<sub>4</sub> emissions from ruminants. We validated an LMD-based approach and then used that approach to evaluate CH<sub>4</sub> emissions from indigenous dairy cows in a dryland area of Ethiopia. Methods: First, we validated our LMD-based approach in Simmental crossbred beef cattle (n = 2) housed in respiration chambers and fed either a high- or low-concentrate diet. From the results of the validation, we constructed an estimation equation to determine CH<sub>4</sub> emissions from LMD CH<sub>4</sub> concentrations. Next, we used our validated LMD approach to examine CH<sub>4</sub> emissions in Fogera dairy cows grazed for 8 h/d (GG, n = 4), fed indoors on natural-grassland hay (CG1, n = 4), or fed indoors on Napier-grass (Pennisetum purpureum) hay (CG2, n = 4). All the cows were supplemented with concentrate feed. Results: The exhaled CH<sub>4</sub> concentrations measured by LMD were linearly correlated with the CH<sub>4</sub> emissions determined by infrared-absorption-based gas analyzer (r<sup>2</sup> = 0.55). The estimation equation used to determine CH<sub>4</sub> emissions (y, mg/min) from LMD CH<sub>4</sub> concentrations (x, ppm m) was y = 0.4259x+38.61. Daily CH<sub>4</sub> emissions of Fogera cows estimated by using the equation did not differ among the three groups; however, a numerically greater milk yield was obtained from the CG2 cows than from the GG cows, suggesting that Napier-grass hay might be better than natural-grassland hay for indoor feeding. The CG1 cows had higher CH<sub>4</sub> emissions per feed intake than the other groups, without significant increases in milk yield and body-weight gain, suggesting that natural-grassland hay cannot be recommended for indoor-fed cows. Conclusion: These findings demonstrate the potential of using LMDs to valuate feeding regimens rapidly and economically for dairy cows in areas under financial constraint, while taking CH<sub>4</sub> emissions into consideration.
Gi Won Choi,Alebel Mekuriaw Abebe,Jin Woo Choi,Je Min Lee 한국원예학회 2021 한국원예학회 학술발표요지 Vol.2021 No.10
Bacterial wilt, caused by the Ralstonia pseudosolnacearum species complex (RSSC), is an economically significant vascular disease that limits tomato production in tropical and subtropical regions. Two major quantitative trait loci (QTL) of bacterial wilt resistance on chromosome 6 (Bwr-6) and 12 (Bwr-12) were previously identified in Solanum lycopersicum ‘Hawaii 7996’; however, marker-assisted breeding for bacterial wilt resistance is not well established. To dissect the QTL, seven markers were developed based on the SNPs near the two QTLs from whole genome resequencing data. Six cleaved amplified polymorphic sites (CAPS) and derived CAPS(dCAPS) markers within the Bwr-6 region and dCAPS marker near Bwr-12 were developed, and resistance levels in 117 tomato cultivars were evaluated. Two markers, RsR6-5 on chromosome 6 and RsR12-1 on chromosome 12, were selected based on the genotypic and phenotypic analysis. The combination of Ch6-5 and Ch12-1 effectively distinguish resistant and susceptible cultivars. Furthermore, the efficiency of the two markers was validated in the F₃ generation derived from the F₂ population between E6203(susceptible) and Hawaii 7996 (resistant). Resistant alleles at both loci led to the resistance to bacterial wilt. These markers will facilitate marker-assisted breeding of tomato resistant to bacterial wilt.
Sick at work: prevalence and determinants among healthcare workers, western Ethiopia
Tesfaye Hambisa Mekonnen,Mekuriaw Alemayewu Tefera,Yayehirad Alemu Melsew 대한직업환경의학회 2018 대한직업환경의학회지 Vol.30 No.-
Background: Going to work despite feeling sick also known as sickness presenteeism is one of the emerging global occupational health challenges. Sickness presenteeism negatively affects both health of work forces and productivity of organizations in general. However, there is insufficient research exploring this situation in majority of the Sub-Saharan African countries, including Ethiopia. Thus this study intended to investigate the prevalence and determinant factors of sickness presenteeism among health care workers, Western Ethiopia. Methods: This study used an institution based cross-sectional quantitative study design. The study period was from February to March, 2017. We employed simple random sampling method to select 360 study samples. Data collection was performed by pre-tested structured and self- administered questionnaire. We used SPSS version 20 to carry out binary logistic regression analysis. Odds ratio with 95% confidence intervals was calculated and significance of associations was determined at p-value < 0.05. Results: A total of 344 respondents fully completed the survey questionnaire. Mean age with standard deviation was 30. 28 ± 6.181. Prevalence of sickness Presenteeism was 52.6% [95%CI: (47.4, 57.8)] in the past 12 months. Educational status [AOR:2.1, 95%CI: (1.17,3.90)], financial problem [AOR:1.9,95%CI:(1.07,3.46)], sickness absenteeism [AOR:2.7,95% CI:(1.50,5.02)], lack of staff replacement [AOR:2.7,95%CI:(1.50,5.02)], absence of occupational health services [AOR:3.0,95%CI:(1.34,6.70)], and pressure from supervisor [AOR:1.8,95% CI:(1.01,3.31)] were significant predictors of the dependent variable. Conclusion: Relatively higher proportions of workers indicated sickness presenteeism as compared to other studies. Risk factors like educational status, personal financial problem, sickness absenteeism, lack of staff replacement, absence of occupational health services, and pressure from supervisors considerably increased the likely occurrence of employees’ sick attendance. It is advisable for health care managers to hire adequate health care staffs, to implement basic occupational health services and to design strategies which reduce pressure from supervisors.