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Qing-Mei Quan,Qing-Xia Wang,Xue-Li Zhou,Shan Li,Xiao-Ling Yang,Yun-Guo Zhu,Zhou Cheng 한국미생물학회 2014 The journal of microbiology Vol.52 No.2
Ophiocordyceps sinensis (Ascomycota: Ophiocordycipitaceae)is a native fungal parasite of Hepialidae caterpillars and oneof the most economically important medicinal caterpillarfungi in China. However, little is known about the phylogeneticand evolutionary relationships between O. sinensis andits host insects. In this study, nuclear ITS and β-tubulin sequencesfrom O. sinensis and mitochondrial COI, COII, andCytb sequences from its hosts were analyzed across 33 populationssampled from five regions in China. Phylogenetically,both O. sinensis and its hosts were divided into three geographicallycorrelated clades, and their phylogenies were congruent. Analysis of molecular variance and calculated coefficientsof genetic differentiation revealed significant geneticdivergence among the clades within both O. sinensis (FST=0.878, NST=0.842) and its hosts (FST=0.861, NST=0.816). Estimatedgene flow was very low for O. sinensis (Nm=0.04) andthe host insects (Nm=0.04) among these three clades. Manteltests demonstrated a significant correlation (P<0.01) betweenthe genetic distances for O. sinensis and its hosts, as well as asignificant association (P<0.05) between geographic and geneticdistances in both. The similar phylogenetic relationships,geographic distributions, and genetic structure and differentiationbetween O. sinensis and its hosts imply that they have coevolved.
Qing-Qing Dong,Qiu-Tong Wang,Lei Wang,Ya-Xin Jiang,Mei-Ling Liu,Hai-Jie Hu,Yong Liu,Hao Zhou,Hong-Peng He,Tong-Cun Zhang,Xuegang Luo 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.4
Sulforaphane (SFN), a natural compound derived from cruciferous vegetables, has been proved to possess potent anti-cancer activity. SMYD3 is a histone methyltransferase which is closely related to the proliferation and migration of cancer cells. This study showed that SFN could dose-dependently induce cell cycle arrest, stimulate apoptosis, and inhibit proliferation and migration of gastric carcinoma cells. Accompanied with these anticancer effects, SMYD3 and its downstream genes, myosin regulatory light chain 9, and cysteine-rich angiogenic inducer 61, was downregulated by SFN. Furthermore, overexpression of SMYD3 via transfection could abolish the effects of SFN, suggesting that SMYD3 might be an important mediator of SFN. To the best of our knowledge, this is the first report describing the role of SMYD3 in the anti-cancer of SFN. These findings might throw light on the development of novel anti-cancer drugs and functional food using SFN-rich cruciferous vegetables.
Qing Zhong Li,Qing Xian Huang,Shu Cui Li,Mei Zi Yang,Bin Rao 대한약리학회 2012 The Korean Journal of Physiology & Pharmacology Vol.16 No.5
A simple, sensitive and reproducible high-performance liquid chromatography (HPLC) method has been validated for determining concentrations of glutamate, glycine, and alanine in human plasma. Proteins in plasma were precipitated with perchloric acid, followed by derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC). Simultaneous analysis of glutamate, glycine, and alanine is achieved using reversed-phase HPLC conditions and ultraviolet detection. Excellent linearity was observed for these three amino acids over their concentration ranges with correlation coefficients (r)>0.999. The intra- and inter-day precision were below 10%. This method utilizes quality control samples and demonstrates excellent plasma recovery and accuracy. The developed method has been successfully applied to measure plasma glutamate, glycine, and alanine in twenty volunteers.
Qing-Mei Guo,Guoli Huang,Xin-Ying Yin,Jian-Jian Bian,Xiao-Mei Li,Ming-Yu Teng 대한화학회 2020 Bulletin of the Korean Chemical Society Vol.41 No.12
Three iridium complexes of (dfppy)2Ir(DAPB), (dfppy)2Ir(DAPB), and (dfppy)2Ir(DAPB) were synthesized by using phosphine carbonylimide derivative as auxiliary ligand and fluoro?/trifluoromethyl substituted 2-phenylpyridine derivatives as primary ligands. Their quantum efficiency range from 19.4% to 38.5%, and show strong rigidochromic effect. The maximum emission wavelengths of solid-state fluorescence have remarkable red shifts from 39 to 55?nm compared with these in CH2Cl2.
Qing Zhong Li,Qing Xian Huang,Shu Cui Li,Mei Zi Yang,Bin Rao 대한생리학회-대한약리학회 2012 The Korean Journal of Physiology & Pharmacology Vol.15 No.5
A simple, sensitive and reproducible high-performance liquid chromatography (HPLC) method has been validated for determining concentrations of glutamate, glycine, and alanine in human plasma. Proteins in plasma were precipitated with perchloric acid, followed by derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC). Simultaneous analysis of glutamate, glycine, and alanine is achieved using reversed-phase HPLC conditions and ultraviolet detection. Excellent linearity was observed for these three amino acids over their concentration ranges with correlation coefficients (r)>0.999. The intra- and inter-day precision were below 10%. This method utilizes quality control samples and demonstrates excellent plasma recovery and accuracy. The developed method has been successfully applied to measure plasma glutamate, glycine, and alanine in twenty volunteers.
Description of 42 unrecorded bacterial species in Korea, belonging to the class Alphaproteobacteria
Qing Mei Liu,김승범,Jung-Hoon Yoon,KISEONG JOH,성치남,Che Ok Jeon,김원용,김명겸,임완택 국립생물자원관 2019 Journal of species research Vol.8 No.4
Here we describe indigenous prokaryotic species in Korea, a total of 42 bacterial strains affiliated to the class Alphaproteobacteria isolated from various environmental samples: fermented vinegar, sea water, beach sand, fresh water, salt flats, moss, algae, activated sludge, and soil. From the high 16S rRNA gene sequence similarity (>98.7%) and formation of a robust phylogenetic clade with the closest species, it was determined that each strain belonged to predefined bacterial species. There is no official report that these 42 species included in Alphaproteobacteria in Korea: 15 species of 6 genera in the order Rhodospirillales, 12 species of 10 genera in the order Rhizobiales, 10 species of 8 genera in the order Rhodobacterales, 4 species of 4 genera in the order Sphingomonadales and 1 species of 1 genus in the order Caulobacterales. Gram reaction, colony and cell morphology, basic biochemical characteristics, isolation source, and strain IDs are also described in the species description section.
( Si Mei Qing ) 한국정치평론학회 2012 정치와 평론 Vol.11 No.-
Arc of Empire focuses on America`s four wars in Asia - in the Philippines, against Japan, in Korea, and in Vietnam -, and the problem of empire. For the first time in the Western literature, these four wars were not treated as ``separate and unconnected.`` Rather, they were considered as “phases in a U.S. attempt to establish and maintain a dominant position in eastern Asia sustained over some seven decades against considerable resistance.” Furthermore, for the first time in the Western literature, the authors tackles “the tough issues of what happens when an imperial project collapses and of what insights the U.S. Asian imbroglio offers on the current U.S. involvement in the Middle East and Central Asia.” This book should have important and far-reaching implications for current American debates on US foreign policy toward East Asia or the new security strategy of ``returning to Asia.``
Zhang, Qing-Mei,Shen, Ning,Xie, Sha,Bi, Shui-Qing,Luo, Bin,Lin, Yong-Da,Fu, Jun,Zhou, Su-Fang,Luo, Guo-Rong,Xie, Xiao-Xun,Xiao, Shao-Wen Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8
Melanoma-associated antigen (MAGE) family genes have been considered as potentially promising targets for anticancer immunotherapy. MAGED4 was originally identified as a glioma-specific antigen. Current knowledge about MAGED4 expression in glioma is only based on mRNA analysis and MAGED4 protein expression has not been elucidated. In the present study, we investigated this point and found that MAGED4 mRNA and protein were absent or very lowly expressed in various normal tissues and glioma cell line SHG44, but overexpressed in glioma cell lines A172,U251,U87-MG as well as glioma tissues, with significant heterogeneity. Furthermore, MAGED4 protein expression was positively correlated with the glioma type and grade. We also found that the expression of MAGED4 inversely correlated with the overall methylation status of the MAGED4 promoter CpG island. Furthermore, when SHG44 and A172 with higher methylation were treated with the DNA demethylating agent 5-aza-2'-deoxycytidine (5-AZA-CdR) reactivation of MAGED4 mRNA was mediated by significant demethylation in SHG44 instead of A172. However, 5-AZA-CdR treatment had no effect on MAGED4 protein in both SHG44 and A172 cells. In conclusion, MAGED4 is frequently and highly expressed in glioma and is partly regulated by DNA methylation. The results suggest that MAGED4 might be a promising target for glioma immunotherapy combined with 5-AZA-CdR to enhance its expression and eliminate intratumor heterogeneity.
한반도에서 미국과 중국의 대결: 위기의 시대에 양국은 서로를 어떻게 평가했는가?
시메이 ( Mei Qing Si ),김기근 한국정치평론학회 2012 정치와 평론 Vol.10 No.-
Before the outbreak of the Korean War, neither Washington nor Beijing intended to engage in a war with the other. By the end of 1950, China and the United States were at war in Korea. In a time of crisis, how did both sides assess each other`s intentions? What historical lessons can we draw from the U.S.-China military confrontation in the Korean crisis? In an attempt to answer these questions, this essay will examine the shift in the Truman administration`s military strategy from the adoption of the defensive perimeter to the decision to cross the 38th parallel in Korea. Doing so will provide important insights on the legacy of President Harry S. Truman in Northeast Asia.