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Md. Abdul Kayum,Jong-In Park,Nasar Uddin Ahmed,Gopal Saha,Ill-Sup Nou 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
Efficient infiltration of water through cell membranes is arbitrated by a family of transmembrane water channels called aquaporins (AQPs). Aquaporin belongs to a highly conserved group of membrane proteins called major intrinsic proteins that facilitate the transport of water and a variety of low molecular weight solutes across biological membranes,which is essential for plants to survive in stress conditions. This study identified 59 BrAQP genes from B. rapa database and Br135K microarray dataset, which was formed by applying low-temperature stresses to contrasting Chinese cabbage two inbreed lines, Chiifu and Kenshin. Based on phylogenetic analyses of BrAQPs revealed four distinct subfamilies, such as plasma membrane intrinsic proteins (PIP), tonoplast intrinsic proteins (TIP), NOD26-like intrinsic proteins (NIP), small basic intrinsic proteins (SIP) with aquaporin of Tomato and Arabidopsis thaliana. All BrAQP genes were firstly examined through homology study with existing biotic and abiotic stress resistance-related aquaporin genes of other plant species and found a high degree of homology. We selected PIP subfamily genes for expression analysis based on microarray data with high and differential transcript abundance levels and homology study with stress related aquaporin genes of other plant species. In our study, we characterized all B. rapa aquaporin genes and understanding the BrPIP subfamily gene function in plants under various environmental stimuli, the expressions of BrPIP genes under various abiotic stress conditions including cold, drought, salinity, water logging, ABA treatment and Fusarium oxysporum f. sp. Conglutinans infection were investigated by a quantitative real-time reverse transcription-PCR analysis. In our expression analysis, 4 BrPIP genes showed responsive expression against F. oxysporum f. sp. Conglutinans infection. The selected genes showed an organ-specific expression, and 12 out of 22 BrPIP genes were differentially expressed in Chiifu compared to Kenshin under cold stresses. Only 7 genes showed up regulation under drought stress and incase of salt stress 17 BrPIP genes were more responsiveness. Additionally, 18 BrPIP genes were up regulated by ABA treatment and all BrPIP genes showed down regulation under water logging stress. Together with expression and bioinformatic analyses, our results provides novel basis to allocate the stress-related biological function to each PIP gene.
Md Abdul Ahad,Mst Kamrun Nahar,Md Ruhul Amin,Sang Jae Suh,Yong Jung Kwon 한국응용곤충학회 2015 한국응용곤충학회지 Vol.54 No.2
Mimosa pudica (미모사), Argemone mexicana (멕시코 가시양귀비) Leucus aspara (꿀풀과 일종), Polygonum hydropiper (여뀌), Blumea lacera (국화과 일종) 등 5종의 식물 헥산추출물들의 팥바구미 성충에 대한 살충, 성충우화억제력 및 녹두 종실피해 방제력이 검증되었다. 그 결과, 밭바구미 성충 살충력은 35-69%이었으며, 녹두에 추출물을 처리한 후 성충우화 방제율은 33-63%, 종실 피해 방제율은 13-49%이었다. 추출물의 농도가 증가할수록 살충력, 성충우화 및 종실피해 방제율 등이 증가하는 것으로 나타났다. 이와 같이 5종의 식물 헥산추출물들은 밭바구미 친환경 방제제로 이용가능성이 있을 것으로 판단된다. This study was conducted with n-hexane extracts of sensitive plant Mimosa pudica, mexican poppy Argemone mexicana, panimarich Leucus aspara, water pepper Polygonum hydropiper and shialmutra Blumea lacera weeds against pulse beetle Callosobruchus chinensis (Coleoptera: Bruchidae) for protection of mung bean Vigna radiata grains. The LC50 values of the weed extracts ranged from 4.5 to 6.4, 4.1 to 5.6 and 3.6 to 5.5 g/100 mL at 24, 48 and 72 hours of post treatment, respectively. The extracts showed 35 to 69% fecundity and 33 to 63% adult emergence inhibitory effect on the pest, and revealed 13 to 49% grain protection of mung beans. Insect mortality, fecundity and adult emergence inhibitory effects, and grain protection activity increased with increased concentration of the extracts. The shialmutra followed by water pepper extracts revealed better performances in fecundity and adult beetle emergence inhibitory effect compared to the other weeds. The findings proved that the n-hexane extracts of the five weeds are sources of botanical insecticides which may be used in the integrated management of C. chinensis.
Md. Abdul Kayum,Hee-Jeong Jung,Jong-In Park,Ill-Sup Nou 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
The Alfin-like transcription factor family is one of the important gene families in eukaryotic plants. They are involved in many biological processes, such as lignocellulosic wall biosynthesis, meristem development, metabolite transport, and responses to biotic and abiotic stresses. But the regulatory mechanism of these genes involved in stresses responses is still unrevealed. In this study, we identified a total of 16 Alfin-like genes from Brassica rapa database. The 16 putative Alfin-like proteins were divided into four groups (group I-IV) based on structural and phylogenetic analyses. Accordingly, this study analyzed stress resistance-related functions of all B. rapa Alfin-like (BrAL) genes through a homology study with existing biotic and abiotic stress resistance-related Alfin-like genes of other plant species and found a high degree of similarity with them. Subsequently, these genes were further investigated by real-time quantative PCR under cold, salt and drought stresses and after infection with Fusarium oxysporum f. sp. conglutinans in B. rapa. These genes showed an organ specific expression and all genes differentially expressed in Chiifu compared to Kenshin under cold stress. Ten and seven BrALs responded highly in Kenshin compared to Chiifu under salt and drought stresses respectively. In addition, six BrAL genes showed responsive expression after Fusarium oxysporum f. sp. conglutinans infection in B. rapa. Interestingly, four BrAL genes showed responses against both biotic and abiotic stress factors. Thus, our result provides a useful reference data set as the basis for functional analysis and utilization in the resistance molecular breeding of B. rapa.
Abdul Murad, Nor Azian,Razak, Zuraini Abdul,Hussain, Rosniza Muhammmad,Syed Hussain, Sharifah Noor Akmal,Ching Huat, Clarence Ko,Siti Aishah, Che Md. Ali,Abdullah, Norlia,Muhammad, Rohaizak,Ibrahim, N Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3
Background: HER-2/neu is a proto-oncogene that encodes a transmembrane tyrosine kinase growth factor which is crucial for stimulating growth and cellular motility. Overexpression of HER-2/neu is observed in 10-35% of human breast cancers and is associated with pathogenesis, prognosis as well as response to therapy. Given the imperative role of HER-2/neu overexpression in breast cancer, it is important to determine the magnitude of amplification which may facilitate a better prognosis as well as personalized therapy in affected patients. In this study, we determined HER-2/neu protein expression by immunohistochemistry (IHC) concurrently with HER-2/neu DNA amplification by quantitative real time-polymerase chain reaction (Q-PCR). Materials and Methods: A total of 53 paired tissue samples from breast cancer patients were frozen-sectioned to characterize the tumour and normal tissues. Only tissues with 80% tumour cells were used in this study. For confirmation, Q-PCR was used to determine the HER-2/neu DNA amplification. Results: We found 20/53 (37.7%) of the tumour tissues to be positive for HER-2/neu protein overexpression using IHC. Out of these twenty, only 9/53 (17%) cases were in agreement with the Q-PCR results. The concordance rate between IHC and Q-PCR was 79.3%. Approximately 20.7% of positive IHC cases showed no HER-2/neu gene amplification using Q-PCR. Conclusion: In conclusion, IHC can be used as an initial screening method for detection of the HER-2/neu protein overexpression. Techniques such as Q-PCR should be employed to verify the IHC results for uncertain cases as well as determination of HER-2/neu gene amplification.