Dietary Supplementation of Carob and Whey Modulates Gut Morphology, Hemato-Biochemical Indices, and Antioxidant Biomarkers in Rabbits

Kaïs Rtibi,Khouloud Marzouki,Abedrraouf Salhi,Hichem Sebai 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.10

This study aimed to evaluate the influence of the carob powder (CP) and sweet whey powder (WhP) inclusion into weaning feed on the gut morphology, hemato-biochemical parameters, and antioxidant biomarkers. The addition of 10 g/kg (basal diet +10 g/kg of CP, of WhP) or the mixture (5 g/kg of CP and 5 g/kg of WhP) in the rabbit's standard diet was assessed. A total of 40 weaned New Zealand White rabbits (4 weeks old) was distributed according to body weight (BW) into four treatments (n = 10) and the feed additives were provided for 7 weeks. Tissue samples and blood were obtained after slaughter. Final BW, daily weight gain, feed conversion ratio, intestinal morphology, and carcass dressing were positively affected by CP–WhP treatments compared with the control diet. Also, CP–WhP treatments significantly increased total proteins, calcium and iron levels, fecal cholesterol excretion, total antioxidants capacity, superoxide dismutase, and catalase in different tissues and significantly decreased total cholesterol, triglycerides, and glucose in blood serum. These changes were associated with a diminution of blood tumor necrosis factor alpha, lipid peroxidation, and carbonyl proteins in rabbit tissues. Both the additives separately and especially in the mix may enhance productive performance, protein profile, gut function, immunity, and antioxidant activity, with reducing lipid peroxidation, essential inflammatory mediator, and protein-carbonyl residues of growing rabbits. These findings suggest that CP–WhP dietary supplementation provides novel insights into a variety of bioactive compound mixtures with different beneficial modes of actions.

Hepatoprotective and Renoprotective Effects of Lavender (Lavandula stoechas L.) Essential Oils Against Malathion-Induced Oxidative Stress in Young Male Mice

Slimen Selmi,Manel Jallouli,Najoua Gharbi,Lamjed Marzouki 한국식품영양과학회 2015 Journal of medicinal food Vol.18 No.10

We aimed in the present study to investigate the hepato- and nephroprotective effects of Lavandula stoechas essential oils (LSEO) against malathion-induced oxidative stress in young male mice as well as the possible mechanism implicated in such protection. Animals were divided into eight groups of 12 each: Control, malathion (200 mg/kg b.w.); Various doses of LSEO (10, 30, and 50 mg/kg b.w.), malathion + various doses of LSEO. Malathion and LSEO were daily per orally (p.o.) administered by intragastric gavage during 30 days. We initially found that malathion treatment induced body weight gain decrease as well as a clear nephro- and hepatotoxicity as assessed by significant relative liver and kidney weight increase and related hemodynamic parameters deregulation. Malathion exposure of mice also induced a considerable perturbation of metabolic parameters. On the other hand, we showed that malathion administration was accompanied by an oxidative stress status assessed by an increase of malondialdehyde (MDA) and hydrogen peroxide (H2O2) levels as well as a depletion of sulfhydril group content (-SH) and antioxidant enzyme activities such as catalase (CAT) and glutathione peroxidase (GPx), total superoxide dismutase (SOD), Cu/Zn-SOD, Mn-SOD, and Fe-SOD in the kidney and liver. More importantly, LSEO treatment abolished all malathion-induced body gain loss, liver and kidney relative weight increase, hemodynamic and metabolic disorders, as well as hepatic and renal oxidative stress. In conclusion, our data suggest that LSEO exerted potential hepato- and nephroprotective effects against malathion-induced oxidative stress in mice. The beneficial effect of LSEO might be related, in part, to its antioxidant properties.

Effects of mining activities on Nano-soil management using artificial intelligence models of ANN and ELM

Liu, Qi,Peng, Kang,Zeng, Jie,Marzouki, Riadh,Majdi, Ali,Jan, Amin,Salameh, Anas A.,Assilzadeh, Hamid Techno-Press 2022 Advances in nano research Vol.12 No.6

Mining of ore minerals (sfalerite, cinnabar, and chalcopyrite) from the old mine has led in significant environmental effects as contamination of soils and plants and acidification of water. Also, nanoparticles (NP) have obtained global importance because of their widespread usage in daily life, unique properties, and rapid development in the field of nanotechnology. Regarding their usage in various fields, it is suggested that soil is the final environmental sink for NPs. Nanoparticles with excessive reactivity and deliverability may be carried out as amendments to enhance soil quality, mitigate soil contaminations, make certain secure land-software of the traditional change substances and enhance soil erosion control. Meanwhile, there's no record on the usage of Nano superior substances for mine soil reclamation. In this study, five soil specimens have been tested at 4 sites inside the region of mine (<100 m) to study zeolites, and iron sulfide nanoparticles. Also, through using Artificial Neural Network (ANN) and Extreme Learning Machine (ELM), this study has tried to appropriately estimate the mechanical properties of soil under the effect of these Nano particles. Considering the RMSE and R2 values, Zeolite Nano materials could enhance the mine soil fine through increasing the clay-silt fractions, increasing the water holding capacity, removing toxins and improving nutrient levels. Also, adding iron sulfide minerals to the soils would possibly exacerbate the soil acidity problems at a mining site.

Cloning and molecular characterization of a new fungal xylanase gene from Sclerotinia sclerotiorum S2

( Olfa Elleuch Ellouze ),( Sana Loukil ),( Mohamed Nejib Marzouki ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.10

Sclerotinia sclerotiorum fungus has three endoxylanases induced by wheat bran. In the first part, a partial xylanase sequence gene (90 bp) was isolated by PCR corresponding to catalytic domains (β 5 and β 6 strands of this protein). The high homology of this sequence with xylanase of Botryotinia fuckeliana has permitted in the second part to amplify the XYN1 gene. Sequence analysis of DNA and cDNA revealed an ORF of 746 bp interrupted by a 65 bp intron, thus encoding a predicted protein of 226 amino acids. The mature enzyme (20.06 kDa), is coded by 188 amino acid (pI 9.26). XYN1 belongs to G/11 glycosyl hydrolases family with a conserved catalytic domain containing E86 and E178 residues. Bioinformatics analysis revealed that there was no Asn-X-Ser/Thr motif required for N-linked glycosylation in the deduced sequence however, five O-glycosylation sites could intervene in the different folding of xylanses isoforms and in their secretary pathway. [BMB reports 2011; 44(10): 653-658]

Management of the energy harvesting for MEMS/NEMS via newmark current method

Shang, Kun,Shan, Huafeng,Alkhalaf, Salem,Marzouki, Riadh,Khadimallah, Mohamed Amine Techno-Press 2022 Advances in nano research Vol.12 No.6

The free and forced vibration in addition to electric energy harvesting of a piezoelectric disk resting on two-parameter foundation modeled by modified couple stress as well as Kirchhoff plate theory is probed. The governing equations and boundary conditions are obtained using Hamilton's principle. Then, the free and forced vibration are solved using numerical solutions, generalized differential quadrature method (GDQM) and Newmark-beta method. The forced vibration is resulted from a base excitation load. Also, the possible voltage which can be harvested from this system is obtained using generalized integral quadrature method. The validity of the formulation and solution procedure is confirmed using a compassion study. The impact of parameters such as length effect, inner to outer radius ratio, and foundations parameters on the free and forced vibration as well as energy harvesting is investigated in detail. This paper can be a basis for future studies in the area of piezoelectric harvesters in small scales.

Strength and permeability of fiber-reinforced concrete incorporating waste materials

Xu, Yun,Xu, Yin,Almuaythir, Sultan,Marzouki, Riadh Techno-Press 2022 Advances in concrete construction Vol.13 No.2

Ecological issues such as natural resource reduction and enormous waste disposals are increasingly leading in developing civilization toward sustainable construction. The two primary environmental issues are the depletion of natural resources and the disposal of trash in open landfills. Waste steel fiber (WSF) was investigated for usage as a cement-based concrete (CBC) constituent in this research. Recycling waste fibers both makes cement composites more long and cost-effective, also aids in pollution reduction. The objective of this study is to analyze the impacts of waste fiber on the fresh and mechanical features of concrete using recycled additives. A comparative research on the durability and mechanical qualities of fiber-reinforced concrete (FRC) constructed with natural aggregates was conducted for this aim. The obstacles to successful WSF recycling methods application in the building industry have been investigated, resulting that CBCs with these fibers make an economic and long lasting choice to deal with waste materials. The workability of fiber enhanced concrete was found to be comparable to that of normal concrete. Fibers have a considerable impact on the splitting tensile strength, flexural and compressive strength of recycled concrete. Fiber may enhance the water permeability. When the WSF content is 0.6 kg/m3, the water absorption is nearly half. Fibers would have no effect on its permeability.

Comparison of postural control between subgroups of persons with nonspecific chronic low back and healthy controls during the modified Star Excursion Balance Test

Shallan, Amjad,Lohman, Everett,Alshammari, Faris,Dudley, Robert,Gharisia, Omar,Al-Marzouki, Rana,Hsu, Helen,Daher, Noha korean Academy of Physical Therapy Rehabilitation 2019 Physical therapy rehabilitation science Vol.8 No.3

Objective: To compare the postural control between non-specific chronic low back pain (NSCLBP) subgroups and healthy people during dynamic balance performance using a modified Star Excursion Balance Test (mSEBT). Design: Cross-sectional study. Methods: Eighteen NSCLBP subjects (9 active extension pattern [AEP], 9 flexion pattern [FP]), and 10 healthy controls were enrolled in this study. All subjects performed mSEBT on their dominant leg on a force plate. Normalized reach distance and balance parameters, including the center of pressure (COP) displacement and velocity, were recorded. Results: There were significant differences in mean reach distances in both posterolateral and posteromedial (PM) reach directions between AEP and healthy subjects (p<0.001) and between FP and healthy subjects (p<0.001). However, there were no significant differences among the three groups in the anterior reach direction. Also, the results showed no significant differences in mean COP variables (velocity and displacement) between pooled NSCLBP and healthy subjects. However, the subjects were reclassified into AEP, FP and healthy groups and the results showed a significant difference in mean COP velocity in the PM direction between AEP and FP subjects (p=0.048), and between AEP and healthy subjects (p=0.024). Conclusions: The findings in this study highlight the heterogeneity of the individuals with NSCLBP and the importance of identifying the homogenous subgroups. Individuals with AEP and FP experience deficits in dynamic postural control compared to healthy controls. In addition, the findings of this study support the concept of the Multidimensional Classification System.

Insight into the corrosion inhibition property of Artocarpus heterophyllus Lam leaves extract

Jiahong He,Qiang Xu,Guoqiang Li,Qiang Li,Riadh Marzouki,Wenpo Li 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.102 No.-

In this work, the method (water extraction way) was uesd to acquire Artocarpus heterophyllus Lamleaves extract (AHLLE). We refer to the relevant references on the composition of Artocarpus heterophyllusLam leaves and perform Fourier infrared spectroscopy analysis for AHLLE. It can be concluded thatAHLLE contains 2-(2,4-dihydroxyphenyl)-5,7-dihydroxy-4H-chromen-4-one (DPD), (2R,3R)-2-(2,4-dihydroxyphenyl)-3 ,5,7-trihydroxychroman-4-one (DPT), 3-(4-hydroxyphenyl)acrylic acid (HPT), and5-(6-hydroxybenzofuran-2-yl)benzene-1,3-diol (HBB). Quantum chemical calculations (QCC) data andmolecular dynamics (MD) simulations results show that DPD, DPT, HPT, and HBB all have the potentialof excellent corrosion inhibitors. Scanning electron microscope (SEM) results and atomic force microscope(AFM) test results show that after adding AHLLE to H2SO4 environment, the copper surface is stillcorrespondingly flat. In addition, the results of electrochemical experiments indicate that the AHLLE is500 ppm, the anti-corrosion efficiency obtained from electrochemical impedance spectroscopy datacan reach 97.3%. With the temperature augments to 313 K, the corrosion inhibition nature of AHLLEcan still be maintained to 97%. AHLLE is adsorbed onto the Cu surface and belongs to Langmuir monolayeradsorption.

Comparison of postural control between subgroups of persons with nonspecific chronic low back and healthy controls during the modified Star Excursion Balance Test

Amjad Shallan,Everett Lohman,Faris Alshammari,Robert Dudley,Omar Gharisia,Rana Al-Marzouki,Helen Hsu,Noha Daherd 물리치료재활과학회 2019 Physical therapy rehabilitation science Vol.8 No.3

Objective: To compare the postural control between non-specific chronic low back pain (NSCLBP) subgroups and healthy people during dynamic balance performance using a modified Star Excursion Balance Test (mSEBT). Design: Cross-sectional study. Methods: Eighteen NSCLBP subjects (9 active extension pattern [AEP], 9 flexion pattern [FP]), and 10 healthy controls were enrolled in this study. All subjects performed mSEBT on their dominant leg on a force plate. Normalized reach distance and balance parameters, including the center of pressure (COP) displacement and velocity, were recorded. Results: There were significant differences in mean reach distances in both posterolateral and posteromedial (PM) reach directions between AEP and healthy subjects (p<0.001) and between FP and healthy subjects (p<0.001). However, there were no significant differences among the three groups in the anterior reach direction. Also, the results showed no significant differences in mean COP variables (velocity and displacement) between pooled NSCLBP and healthy subjects. However, the subjects were reclassified into AEP, FP and healthy groups and the results showed a significant difference in mean COP velocity in the PM direction between AEP and FP subjects (p=0.048), and between AEP and healthy subjects (p=0.024). Conclusions: The findings in this study highlight the heterogeneity of the individuals with NSCLBP and the importance of identifying the homogenous subgroups. Individuals with AEP and FP experience deficits in dynamic postural control compared to healthy controls. In addition, the findings of this study support the concept of the Multidimensional Classification System.

Gastroprotective and Antioxidant Properties of Trigonella foenum graecum Seeds Aqueous Extract (Fenugreek) and Omeprazole Against Ethanol-Induced Peptic Ulcer

Slimen Selmi,Dhouha Alimi,Kais Rtibi,Saber Jedidi,Dhekra Grami,Lamjed Marzouki,Karim Hosni 한국식품영양과학회 2022 Journal of medicinal food Vol.25 No.5

Trigonella foenum graecum (Fenugreek) is used in traditional phytomedicine for its anti-inflammatory, antiseptic, antidiabetic, and several other therapeutic virtues. The current study was intended to investigate the protecting effects of fenugreek seeds’ aqueous extract (FSAE) using experimentally ethanol (EtOH)-induced gastric peptic ulcer in rats, as immense alcohol consumption can lead to gastric ulcer. Sixty adult male Wistar rats were divided into 6 groups of 10 each: control, EtOH (4 g/kg body weight [b.w.]), EtOH + several doses of FSAE (50, 100, and 200 mg/kg b.w.), and EtOH + Omeprazole (OM, 20 mg/kg orally [p.o.]). Animals were p.o. pretreated with FSAE for 21 days and exposed to a single oral administration of EtOH (4 g/kg b.w.) for 2 h. Gastric ulcer in rats was induced with a single dose of EtOH. Ulcer index, malondialdehyde (MDA), hydrogen peroxide (H2O2), and thiol groups (-SH) content in stomach, and antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were measured. Our recorded results showed that EtOH induced gastric damage, evidenced by the level of oxidative stress markers such as MDA and H2O2 in rats exposed to EtOH. However, significant increases in the activities of antioxidant enzymes were recorded, such as SOD, CAT, and GPx, and a decrease in nonenzymatic antioxidants, such as (-SH). Moreover, histopathological examinations showed the presence of lesions associated with severe tissue damage in the untreated rats. Interestingly, FSAE meaningfully protects against all gastric damages caused by EtOH. We propose that FSAE exhibits protective effects in EtOH-induced peptic ulcer in rats. This protection might be related to its antioxidant and anti-inflammatory properties as well as its opposite effects on some studied intracellular mediators.