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Highly Multiplexed Fluorescence <i>in Situ</i> Hybridization for <i>in Situ</i> Genomics
Onozato, Maristela L.,Yapp, Clarence,Richardson, Douglas,Sundaresan, Tilak,Chahal, Varun,Lee, Jesse,Sullivan, James P.,Madden, Marisa W.,Shim, Hyo S.,Liebers, Matthew,Ho, Quan,Maheswaran, Shyamala,Hab American Society for Investigative Pathology 2019 The Journal of Molecular Diagnostics Vol. No.
<P>The quantification of changes in gene copy number is critical to our understanding of tumor biology and for the clinical management of cancer patients. DNA fluorescence <I>in situ</I> hybridization is the gold standard method to detect copy number alterations, but it is limited by the number of genes one can quantify simultaneously. To increase the throughput of this informative technique, a fluorescent bar-code system for the unique labeling of dozens of genes and an automated image analysis algorithm that enabled their simultaneous hybridization for the quantification of gene copy numbers were devised. We demonstrate the reliability of this multiplex approach on normal human lymphocytes, metaphase spreads of transformed cell lines, and cultured circulating tumor cells. It also opens the door to the development of gene panels for more comprehensive analysis of copy number changes in tissue, including the study of heterogeneity and of high-throughput clinical assays that could provide rapid quantification of gene copy numbers in samples with limited cellularity, such as circulating tumor cells.</P>
Profile of Professionals of the Brazilian Production Sector of Timber Housing
( Victor DE ARAUJO ),( Cesar POLANCO ),( Elen MORALES ),( Juliana CORTEZ-BARBOSA ),( Maristela GAVA ),( Jose GARCIA ) 한국목재공학회 2019 목재공학 Vol.47 No.5
On account of the lack of education of Brazilian worker, this paper analyzed the characteristics of those professionals working in the production sector of timber houses. A sectoral survey was carried out with respective entrepreneurs to investigate the available professionals (career and contract types), evaluate the demands of skilled workers, and indicate solutions to improve the quality of labor qualification. Similarly, over 65% of sampled producers presented both direct-hired and outsourced workforce. For such contract ways, Civil Engineers and Architects were the main careers. Carpenters, Civil Engineers and Architects experienced on timber were the main sectoral demands. Timber Engineers have good potentials of service for this sector. The creation of courses on timber products can emerge as a key alternative to train people.
Unique Genetic and Survival Characteristics of Invasive Mucinous Adenocarcinoma of the Lung
Shim, Hyo Sup,Kenudson, Mari-,Zheng, Zongli,Liebers, Matthew,Cha, Yoon Jin,Hoang Ho, Quan,Onozato, Maristela,Phi Le, Long,Heist, Rebecca S.,Iafrate, A. John Elsevier 2015 JOURNAL OF THORACIC ONCOLOGY Vol.10 No.8
Levels of Supplementation for Grazing Beef Heifers
Cabral, Carla Heloisa Avelino,Paulino, Mario Fonseca,Detmann, Edenio,de Campos Valadares Filho, Sebastiao,de Barros, Livia Vieira,Valente, Eriton Egidio Lisboa,de Oliveira Bauer, Maristela,Cabral, Car Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.6
The objective of this experiment was to evaluate the effect of providing different levels of a supplement on the nutritional characteristics and productive performance of heifers on pasture during the rainy-dry transition and dry season in Brazil or tropical area. Thirty crossbred heifers with predominance of Zebu breed were used in a completely randomized experimental design. Treatments consisted of a mineral supplement and 0.5, 1.0, 1.5, or 2.0 kg/animal/d of a protein supplement containing 300 g crude protein (CP)/kg of dry matter (DM). In the rainy-dry transition season there was quadratic effect of the protein supplementation (p<0.10) on daily weight gain (DWG). A linear relationship (p<0.10) was found between increasing supplement intake and intakes of DM, organic matter (OM), crude protein (CP), ether extract (EE), non fibrous carbohydrates (NFC) and total digestible nutrients (TDN). Coefficients of apparent digestibility of CP, EE, and NFC increased linearly (p<0.10) with increasing supplement levels, but there was no effect on the DM apparent digestibility (p>0.10); the microbial efficiency (g CPmic/kg TDN) and the relationship of microbial nitrogen flow with nitrogen intake (g/g nitrogen intake) were negative linear profiles. In the dry season, the descriptive pattern least squares means showed a trend of stabilization of DWG from the supply of 0.98 kg of protein supplement; the intakes of DM, OM, CP, EE, NFC, and TDN showed increasing linear relationship (p<0.10) with protein supplement levels; the means of apparent digestibility coefficients of the different dietary fractions presented a linear-response-plateau (LRP); the microbial nitrogen flow (g/d) showed positive linear profile (p<0.10) for supplementation levels. It is concluded that supplementation improves the productive performance of grazing heifers and that 1.0 kg/d of supplement per animal gives the maximum increment of weight gain.