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Differential Role of VDR Coactivators SRC3 and MED1 in Regulating Skin Homeostasis
( Lizhi Hu ),( Jing Zhang ),( Maoqiang Man ) 한국피부장벽학회 2018 한국피부장벽학회지 Vol.20 No.2
The vitamin D receptor (VDR) participates in skin homeostasis maintenance by modulating sequential expression of a number of genes involved in proliferation, differentiation, barrier function, and immune response, etc. This process is under precise regulation of coactivators and corepressors, activating or inhibiting the transcription activity of VDR in specific spatio-temporal fashion. In keratinocytes, the major coactivator complexes include the vitamin D receptor interacting protein (DRIP, or Mediator) complex and steroid receptor coactivator (SRC) complex. MED1, a subunit of Mediator that that binds directly to VDR, is abundantly expressed in proliferating keratinocytes. We found that MED1 had a specific role in the regulation of epidermal stem cell self-renewal, keratinocyte proliferation and differentiation ability by modulating the activation of β-catenin signaling pathways. SRC3, on the other hand, regulates the ability of 1,25(OH)2D3 to induce functions in the more differentiated keratinocyte such as lipid synthesis and processing required for permeability barrier formation and innate immune response triggered by disruption of the barrier. Therefore, we provide evidence to show that alternative combination of MED1 or SRC3 with VDR could regulate the on and off of target genes expression, selectively control different VDR functions, and achieve the skin homeostasis maintenance in diverse layers of skin.
Xianjing Zheng,Haimei Liu,Maoqiang Ma,Jianbo Ji,Faliang Zhu,Longru Sun 대한약학회 2021 Archives of Pharmacal Research Vol.44 No.4
Over the past 100 years, Salvia miltiorrhiza f. alba (Lamiaceae) (RSMA) roots have been used to curethromboangiitis obliterans (TAO) in local clinics. This studyaimed to confi rm the anti-thrombotic effi cacy of 12 phenolicacids obtained from RSMA and to clarify the possibleunderlying mechanisms. The results of quantitative real-timepolymerase chain reaction (qRT-PCR) and enzyme-linkedimmunosorbent assay (ELISA) experiments demonstratedthat most of the phenolic acids markedly inhibited PAI-1protein and mRNA levels but increased t-PA protein andmRNA levels in TNF-α-induced EA.hy926 cells ( P < 0.05or 0.001), with lithospermic acid displaying the strongesteff ect. In vitro anticoagulation and antiplatelet aggregationassays showed that lithospermic acid and salvianolic acidB signifi cantly prolonged prothrombin time (PT), activatedpartial thromboplastin time (APTT), decreased fi brinogen concentration (FIB), and inhibited platelet aggregationinduced by adenosine diphosphate (ADP) in rat blood. Bothlithospermic acid and salvianolic acid B markedly down-regulatedthe expression of factor Xa and factor IIa on the externalsurface of EA.hy926 cells and demonstrated signifi cantanti-factor IIa and anti-factor Xa activity using chromogenicsubstrates in vitro. Western blot results revealed that bothlithospermic acid and salvianolic acid B also signifi cantlyinhibited the expression of TF, p-p65, p-p38, and pJNK proteinsinduced by TNF-α. These results indicated that all ofthe phenolic acids appeared to have some anti-thromboticactivity, with salvianolic acid B and lithospermic acid markedlydecreasing the chance of thrombosis by regulating theNF-κB/JNK/p38 MAPK signaling pathway in response toTNF-α.
Jiang Tianyan,Deng Biao,He Qiaoqing,Bi Maoqiang,Chen Xi,Bao Lianwei,Zhou Xin 대한전기학회 2023 Journal of Electrical Engineering & Technology Vol.18 No.2
This paper presents Weibull failure models by experimental work and the step-up accelerated electrical–thermal test of oil-impregnated paper with 0.2 mm thickness is carried out under pulsating DC voltage at different temperatures. The breakdown voltage and failure time of the oil gap–paper insulation specimens at the same voltage duration is obtained by accelerated electrical–thermal aging test. The Weibull failure models of three-variable oil gap–paper insulation modified by Fallou and Ramu are established to analyze the failure data of oil gap–paper under different electric field intensities and temperatures. The statistical test method was used to evaluate the applicability and accuracy of two modified triple-variables Weibull failure models. The comprehensive test index of the Fallou-modified WFD is 1.6851, and the comprehensive test index of the Ramu-modified WFD is 1.8124. The Fallou-modified results were more accurate than Ramu-modified results in estimating the statistical distribution of failure data. Results show that the proposed triple-variable Weibull failure model could fully reflect the insulation state of oil gap–paper under the action of electric and thermal fields.
Jiang Tianyan,Zhang Tao,Li Yang,Zeng Shuai,Liu Qiang,Bi Maoqiang 대한전기학회 2023 Journal of Electrical Engineering & Technology Vol.18 No.4
The safe operation of power transformer is vital to the reliability of modern power network. The bushing is a key component of transformer that serves to connect the transformer windings to the transmission lines. Due to its intricate structure and severe operating conditions, the bushing is among the most frequent cause of transformer failure. Therefore, it is essential to monitor the condition of transformer bushing for maintaining the safe and stable operation of grid. In this paper, we propose a graphene piezoresistive pressure sensor based on handkerchief paper (GHPPS) to monitor the internal pressure of transformer bushing. The GHPPS possesses a highly porous structure and a fibrous tissue, and exhibits a superb sensitivity to the variations of bushing internal pressure. Moreover, we compared the sensitivity and conductivity of the sensors with different paper layers. The GHPPS with 8 layers owns the highest sensitivity (15.6 kPa−1), the smallest response time (60 ms) and recovery time (75 ms), and the best stability. In addition, a hierarchical alarm device is used to test the monitoring capability of GHPPS at different pressure levels. The results prove that the GHPPS is a promising tool for monitoring bushing conditions.
Zhikun Zhang,Ying Zhou,Jing-Kui Yang,Peilong Wang,Xiaoou Su,Hong Zhao,Yujian He,Zhiqin Cao,Maoqiang Luo 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.7
A new method has been proposed to realize the visual detection of Cr3+ using 4-nitrobenzenethiol (4-NBT) and 4-mercaptobenzoic acid (4-MBA) modified silver nanoparticles (AgNPs). The presence of Cr3+ induces the aggregation of AgNPs through cooperative metal–ligand interaction, resulting in a color change from bright yellow to purple. Consequently, Cr3+ could be monitored by colorimetric response of AgNPs by a UV-Vis spectrophotometer or even naked eyes. We firstly used ethylene diamine tetraacetic acid (EDTA) as a masking agent to selectively detect Cr3+, and other metal ions have little influence on the Cr3+–AgNPs system. The cofunctionalized AgNPs exhibited a highly sensitive detection limit of Cr3+, which is as low as 5 x 10-9 mol L-1, and the absorbance ratio (A600nm /A387nm) is linear with the concentration of Cr3+ ranging from 5 x 10-9 mol L-1 to 2 x 10-6 mol L-1 with a coefficient of 0.993. Particularly, the sensor has been further evaluated to monitor the concentration of Cr3+ in drinking water, the recovery was in good agreement with those obtained by ICP-MS, indicating that this proposed method is successfully applied in real samples.