An In-depth Investigation into the Influence of Chatbot Usability and Age on Continuous Intention to Use: A Comprehensive Study

Manigandan L.,Sivakumar Alur 한국경영정보학회 2024 Asia Pacific Journal of Information Systems Vol.34 No.1

This study aims to assess the impact of chatbot usability and demographics on continuous intention to use across different sectors. The research employed Braun’s Bot Usability Scale (BUS11) to measure chatbot usability, focusing on accessibility, quality, conversation quality, privacy risk, and response time. A total of 187 participants completed a survey as part of this study. Variance-based SEM was utilized to examine relationships and test hypotheses. This study contributes to the ongoing discourse on chatbot adoption and user behaviour. It enhances the understanding of chatbot usability, highlighting the role of age in continued intention to use chatbots. The findings suggest that different age groups may possess specific preferences and expectations regarding chatbot usability. These differing preferences can influence their intention to continue using this technology. The study reveals that chatbot usability significantly impacts continuous intention to use and that age moderates the relationship between perceived conversation quality, information, privacy, security, and continuous intention to use. Based on the study’s results, it is recommended that chatbot designers enhance usability to promote long-term adoption and usage.

제브라피쉬 배아의 다양한 단계에서 알로에 추출물의 기형유도 능력 평가

Manigandan Krishnan,Jai Heon Lee,Sun Chul Kang 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.10

Background : Obvious applications of aloe (Aloe vera) and their rapid intake make it crucial to evaluate their biological impacts to aquatic organisms. Methods and Results : In this study, we investigated the possible effects of aloe extract on zebrafish embryos (Danio rerio) by assessing teratogenic properties including survival rate, hatching success, heart rate and morphological changes for 96hpf (hours post fertilization). We noticed that exposure to aloe extracts (0.1, 10, 50, 100 and 150 μl/ml in embryo medium) to zebrafish embryos showed no alterations in survival and morphological changes when compared to control embryos. In contrast, embryos exposed to 150 μl/ml of aloe extract shows delayed hatching rate at 72hpf when compared to lower concentrations. Similarly, aloe extract at 150 μl/ml exposed embryos elicited significantly reduced heart rate (132 ± 1 beats/min) at 48hpf when compare to control embryos. Conclusion : Based on the above findings, we speculate the teratogenic effect of aloe extract on zebrafish embryos.

Anti-Endotoxin 9-Meric Peptide with Therapeutic Potential for the Treatment of Endotoxemia

( Manigandan Krishnan ),( Joonhyeok Choi ),( Sungjae Choi ),( Yangmee Kim ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.1

Inflammatory reactions activated by lipopolysaccharide (LPS) of gram-negative bacteria can lead to severe septic shock. With the recent emergence of multidrug-resistant gram-negative bacteria and a lack of efficient ways to treat resulting infections, there is a need to develop novel anti-endotoxin agents. Antimicrobial peptides have been noticed as potential therapeutic molecules for bacterial infection and as candidates for new antibiotic drugs. We previously designed the 9-meric antimicrobial peptide Pro9-3 and it showed high antimicrobial activity against gram-negative bacteria. Here, to further examine its potency as an anti-endotoxin agent, we examined the antiendotoxin activities of Pro9-3 and elucidated its mechanism of action. We performed a dye-leakage experiment and BODIPY-TR cadaverine and limulus amebocyte lysate assays for Pro9-3 as well as its lysine-substituted analogue and their enantiomers. The results confirmed that Pro9-3 targets the bacterial membrane and the arginine residues play key roles in its antimicrobial activity. Pro9-3 showed excellent LPS-neutralizing activity and LPS-binding properties, which were superior to those of other peptides. Saturation transfer difference-nuclear magnetic resonance experiments to explore the interaction between LPS and Pro9-3 revealed that Trp3 and Tlr7 in Pro9-3 are critical for attracting Pro9-3 to the LPS in the gram-negative bacterial membrane. Moreover, the anti-septic effect of Pro9-3 in vivo was investigated using an LPS-induced endotoxemia mouse model, demonstrating its dual activities: antibacterial activity against gram-negative bacteria and immunosuppressive effect preventing LPS-induced endotoxemia. Collectively, these results confirmed the therapeutic potential of Pro9-3 against infection of gram-negative bacteria.

Tests and numerical behavior of circular concrete-filled double skin steel tubular stub columns under eccentric loads

Manigandan R.,Manoj Kumar 국제구조공학회 2023 Structural Engineering and Mechanics, An Int'l Jou Vol.88 No.3

This article describes experimental and numerical analyses of eccentrically loaded over the axially loaded circular concrete filled double-skinned steel tubular (CFDST) short columns. Tests on circular CFDST short columns under eccentric and concentric loading were conducted to assess their responses to the frequent intensity of 5–30 mm at the interval of each 5 mm eccentric loading conditions with constant cross-sectional proportions and width-to-thickness ratios of the outside and internal tubes. The non-linear finite-element analysis of circular CFDST short columns of eccentrically loaded over the axially loaded was performed using the ABAQUS to predict the structural behavior and compare the concentric loading capacity over the various eccentric loading conditions. The comparison outcomes show that the axial compressive strength of the circular CDFST short columns was 2.38–32.86%, lesser than the concentrically loaded short column with the inner circular section. Also, the influence of computer simulation employed is more efficient in forecasting the experimentally examined performance of circular CFDST stub columns.

The microstructure and mechanical performance of high strength alloy steel X2M

Manigandan, K.,Srivatsan, T.S.,Freborg, A.M.,Quick, T.,Sastry, S. Techno-Press 2014 Advances in materials research Vol.3 No.1

In this paper, the microstructure, hardness, tensile deformation and fracture behavior of high strength alloy steel X2M is presented anddiscussed. The influence of both composition and processing on microstructure of the as-provided material and resultant influence of microstructure, as a function of orientation, on hardness, tensile properties and final fracture behavior is highlighted. The macroscopic mode and intrinsic microscopic features that result from fracture of the steel specimens machined from the two orientations, longitudinal and transverse is discussed. The intrinsic microscopic mechanisms governing quasi-static deformation and final fracture behavior of this high strength steel are outlined in light of the effects oftest specimen orientation, intrinsic microstructural effects and nature of loading.

Investigation on circular and octagonal concrete-filled double skinned steel tubular short columns under axial compression

Manigandan R,Manoj Kumar,Hrishikesh N. Shedge 국제구조공학회 2022 Steel and Composite Structures, An International J Vol.44 No.1

This paper describes the experimental and numerical investigation on circular and octagonal CFDST short columns under concentric loading to study their responses to various internal circular steel tube sizes by the constant cross-sectional dimensions of the external circular and octagonal steel tube. The non-linear finite element analysis of circular and octagonal CFDST columns was executed using the ABAQUS to forecast and compare the axial behavior influenced by the various sizes of internal circular steel tubes. The study shows that the axial compressive strength and ductility of circular and octagonal CFDST columns were significantly influenced by inner steel tubes with the strengths of constituent materials.

Teratogenic Inducing Capacity of Aloe Extract on Different Stages of Zebrafish Embryos

Manigandan Krishnan(마니간단 크리쉬난),Jai Heon Lee(이재헌),Sun Chul Kang(강선철) 한국약용작물학회 2016 한국약용작물학회 학술대회논문집 Vol.2016 No.2

Loss of Lymphocyte Cytosolic Protein 1 (LCP1) Induces Browning in 3T3-L1 Adipocytes via β3-AR and the ERK-Independent Signaling Pathway

Manigandan SUBRAMANI,Jong Won YUN 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10

Lymphocyte cytosolic protein 1 (LCP1) plays a vital role in non-hematopoietic malignancies, and is also a well-known tumor biomarker; however, evidence regarding its function in adipocytes remains to be elucidated. The current study explores the physiological role of LCP1 in cultured 3T3-L1 white adipocytes, by applying the loss-of-function study using siRNA. Induction of fat browning by LCP1 depletion was evidenced by evaluating the gene and protein expression levels of brown fat-associated markers through real-time qRT-PCR and immunoblot analysis, respectively. We observed that deficiency of LCP1 promotes mitochondrial biogenesis, and significantly enhances expressions of the core brown fat-specific genes (Cd137, Cidea, Cited1, Tbx1, and Tmem26) and proteins (PGC-1α, PRDM16, and UCP1). In addition, deficiency of LCP1 promotes lipid catabolism as well as suppresses adipogenesis and lipogenesis. Loss of LCP1 also ameliorates cellular stress by downregulating JNK and c-JUN in adipocytes, and stimulates apoptosis. A mechanistic study revealed that deficiency of LCP1 induces browning in white adipocytes, independently via β3-AR and the ERK signaling pathway. The current data reveals a previously unknown mechanism of LCP1 in browning of white adipocytes, and highlights the potential of LCP1 as a pharmacotherapeutic target for treating obesity and other metabolic disorders.

Shape effect on axially loaded CFDST columns

Manigandan R,Manoj Kumar 국제구조공학회 2022 Steel and Composite Structures, An International J Vol.43 No.6

Concrete-filled double skinned steel tubular (CFDST) columns have been used to construct modern structures such as tall buildings and bridges as well as infrastructures as they provide better, lesser weight, and greater stiffness in structural performance than conventional reinforced concrete or steel members. Different shapes of CFDST columns may be needed to satisfy the architectural and aesthetic criteria. In the study, three-dimensional FE simulations of circular and elliptical CFDST columns under axial compression were developed and verified through the experimental test data from the perspectives of full load-displacement histories, ultimate axial strengths, and failure modes. The verified FE models were used to investigate and compare the structural performance of CFDST columns with circular and elliptical cross-section shapes by evaluating the overall load-deformation curves, interaction stress-deformation responses, and composite actions of the column. At last, the accuracy of available design models in predicting the ultimate axial strengths of CFST columns were investigated. Research results showed that circular and elliptical CFDST column behaviors were generally similar. The overall structural performance of circular CFDST columns was relatively improved compared to the elliptical CFDST column.

ATP1A2 Negatively Regulates UCP1-dependent and UCP1-independent Thermogenesis in 3T3-L1 Adipocytes

Subramani MANIGANDAN,Jong Won YUN 한국생물공학회 2022 한국생물공학회 학술대회 Vol.2022 No.9