Genome‑wide characterization of simple sequence repeats in Palmae genomes

Manee M. Manee,Badr M. Al‑Shomrani,Mohamed B. Al‑Fageeh 한국유전학회 2020 Genes & Genomics Vol.42 No.5

Background Microsatellites or simple sequence repeats (SSRs) have become the most significant DNA marker technology used in genetic research. The availability of complete draft genomes for a number of Palmae species has made it possible to perform genome-wide analysis of SSRs in these species. Palm trees are tropical and subtropical plants with agricultural and economic importance due to the nutritional value of their fruit cultivars. Objective This is the first comprehensive study examining and comparing microsatellites in completely-sequenced draft genomes of Palmae species. Methods We identified and compared perfect SSRs with 1–6 bp nucleotide motifs to characterize microsatellites in Palmae species using PERF v0.2.5. We analyzed their relative abundance, relative density, and GC content in five palm species: Phoenix dactylifera, Cocos nucifera, Calamus simplicifolius, Elaeis oleifera, and Elaeis guineensis. Results A total of 118241, 328189, 450753, 176608, and 70694 SSRs were identified, respectively. The six repeat types were not evenly distributed across the five genomes. Mono- and dinucleotide SSRs were the most abundant, and GC content was highest in tri- and hexanucleotide SSRs. Conclusion We envisage that this analysis would further substantiate more in-depth computational, biochemical, and molecular studies on the roles SSRs may play in the genome organization of the palm species. The current study contributes a detailed characterization of simple sequence repeats in palm genomes.

Visions for the EU’s Federal Foreign Policy: For A Better Future

( Mane Heo ) 한국EU학회 2022 Asia-Pacific Journal of EU Studies Vol.20 No.1

The paper aims to explore a possibility of applying a federal foreign policy in the long tradition of the European integration. It tries to seek a more coordinated common foreign, security and defense policies in its efforts to ensure both more effectiveness and sustainment of CFSP and ESDP in a federal architecture, while it purports to develop European characters in the three fields, with the effectuation of a new Council for Federal Foreign Policy. The EU in the Post-Cold War period faces more uncertainties and challenges both internally and externally. It suggests the compelling necessities to apply federal foreign policy as part of institutional innovations that could move to an ever closer union and that could enhance European characters in the three fields.

Potential of different salt forming agents on the formation of chitosan nanoparticles as carriers for protein drug delivery systems

Manee Luangtana‑anan,Jurairat Nunthanid,Sontaya Limmatvapirat 한국약제학회 2019 Journal of Pharmaceutical Investigation Vol.49 No.1

The effects of salt forming agents for chitosan on the potential for nanoparticle formation was investigated. The salt forms were prepared from the amino acid group, including glutamic and aspartic acids, and the alpha hydroxyl acid group, including lactic and glycolic acids. All types of chitosan salt could be used to prepare bovine serum albumin (BSA) loaded nanoparticles. The chitosan salts prepared from the amino acid group showed a higher salt formation ability as demonstrated using FTIR, hence a higher %encapsulation efficiency (%EE) and a reduction in zeta potential were obtained. The difference was due to the different organic acids used giving different polymer conformations and pH values in solution. Chitosan glutamate gave the highest salt formation ability and hence the highest %EE was obtained. The release of protein from all types of chitosan was similar and chitosan glutamate exhibited the highest release. Chitosan salt is therefore a material of choice for protein-loaded nanoparticles and the characteristics of nanoparticles can be readily modulated by different types of salt form.

Failure in the Denuclearization of the DPRK: Six Gradual Countermeasures and a Way to ‘Carpet Peace’

( Mane Heo ) 한국EU학회 2021 Asia-Pacific Journal of EU Studies Vol.19 No.2

Having taken the bold initiative for the denuclearization of the DPRK, South Korea removed all U.S. tactical nuclear weapons in 1991 under the Roh Tae-Woo government. Inspired by the idea of denuclearization, South Korea has made long, constant efforts to reach a solution to the issue. The sustainable conflict resulted from the issue has continued to damage the conditions for ‘carpet peace’ on the peninsula designed as the highest level of peace. The 1994 Geneva Agreed Framework aimed to freeze the nuclear development programs at Yongbyon, but failed. The September 2005 Six-Party Talks, long chaired by China, did not reach a solution for the dismantling of the nuclear facilities. The February 2017 Agreement aimed at implementing the Joint Statement of September 2005 did not work, either. The KEDO, a multinational consortium to provide North Korea with two light water reactors, broke down. This paper lays out a way to achieve ‘carpet peace’ through six countermeasures to the nuclearization, with the ‘Six-Party Talks +EU’ suggested as one of them.

A Northeast Asian Multilateral Security Cooperation Regime : Retrospects and Prospects

Mane, Heo 부산대학교 과학교육연구소 2003 교사교육연구 Vol.42 No.-

L-carnitine Supplemented Extender Improves Cryopreserved-thawed Cat Epididymal Sperm Motility

Manee-In, S.,Parmornsupornvichit, S.,Kraiprayoon, S.,Tharasanit, T.,Chanapiwat, P.,Kaeoket, K. Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.6

Cryopreservation of epididymal sperm is an effective technique to preserve genetic materials of domestic cats and wild felids when they unexpectedly die. However, this technique inevitably causes detrimental changes of cryopreserved-thawed spermatozoa, for example, by physical damage and excessive oxidative stress. L-carnitine is an antioxidant that has been used to improve sperm motility in humans and domestic animals. This study aimed to investigate the effects of L-carnitine on cat epididymal sperm quality following cryopreservation and thawing. After routine castration, cauda epididymides were collected from 60 cat testes. The epididymal spermatozoa from 3 cauda epididymides were pooled as 1 replicate. Spermatozoa samples (16 replicates) were examined for spermatozoa quality and then randomly divided into 4 groups: 0 mM L-carnitine (control), 12.5 mM, 25 mM and 50 mM L-carnitine. The sperm aliquots were then equilibrated and conventionally frozen. After thawing, sperm motility, plasma membrane integrity, DNA integrity and acrosome integrity were evaluated. The 25 mM L-carnitine significantly improved sperm motility compared with a control group (p<0.05), although this was not significantly different among other concentrations. In conclusion, supplementation of 25 mM L-carnitine in freezing extender improves cauda epididymal spermatozoa motility. The effects of L-carnitine on the levels of oxidative stress during freezing and thawing remains to be examined.

Peak mooring forces in the horizontal interlaced multi-layered moored floating pipe breakwater

Mane, Vishwanath,Rajappa, Sacchi,Rao, Subba,Vittal, Hegde A. The Society of Naval Architects of Korea 2011 International Journal of Naval Architecture and Oc Vol.3 No.2

Present study aims to investigate the influence of relative breakwater width W/L (W=width of breakwater, L=wavelength), wave steepness $H_i/gT^2$ (Hi=incident wave height, T=wave period) and relative wave height d/W (d=water depth) on forces in the moorings of horizontal interlaced multi-layered moored floating pipe breakwater (HIMMFPB) model. Studies were conducted on scaled down physical models having three layers of Poly Vinyl Chloride (PVC) pipes, wave steepness $H_i/gT^2$ varying from 0.063 to 0.849, relative width W/L varying from 0.4 to 2.65 and relative spacing S/D=2 (S=horizontal centre-to-centre spacing of pipes, D=diameter of pipes). Peak mooring forces were also measured and data collected is analyzed by plotting non-dimensional graphs depicting variation of $f_s/{\gamma}W^2$ ($f_s$=Sea side Mooring force, ${\gamma}$=specific weight of water) & $f_l/{\gamma}W^2$ ($f_l$=Lee side Mooring force) with $H_i/gT^2$ for d/W varying from 0.082 to 0.276 and also variation of $f_s/{\gamma}W^2$ and $f_l/{\gamma}W^2$ with W/L for $H_i$/d varying from 0.06 to 0.400.

Effect of Composition and Pretreatment Parameters on Activity and Stability of Cu-Al Catalysts for Water-Gas Shift Reaction

Mane, Rasika B.,Jeong, Dae-Woon,Malawadkar, Atul V.,Roh, Hyun-Seog,Rode, Chandrashekhar V. Wiley Blackwell (John Wiley Sons) 2014 CHEMCATCHEM Vol.6 No.6

Fast response of sprayed vanadium pentoxide (V₂O₅) nanorods towards nitrogen dioxide (NO₂) gas detection

Mane, A.A.,Suryawanshi, M.P.,Kim, J.H.,Moholkar, A.V. Elsevier BV * North-Holland 2017 Applied Surface Science Vol.403 No.-

<P><B>Abstract</B></P> <P>The V<SUB>2</SUB>O<SUB>5</SUB> nanorods have been successfully spray deposited at optimized substrate temperature of 400°C onto the glass substrates using vanadium trichloride (VCl<SUB>3</SUB>) solution of different concentrations. The effect of solution concentration on the physicochemical and NO<SUB>2</SUB> gas sensing properties of sprayed V<SUB>2</SUB>O<SUB>5</SUB> nanorods is studied at different operating temperatures and gas concentrations. The XRD study reveals the formation of V<SUB>2</SUB>O<SUB>5</SUB> having an orthorhombic symmetry. The FE-SEM micrographs show the nanorods-like morphology of V<SUB>2</SUB>O<SUB>5</SUB>. The AFM micrographs exhibit a well covered granular surface topography. For direct allowed transition, the band gap energy values are found to be decreased from 2.45eV to 2.42eV. The nanorods deposited with 30mM solution concentration shows the maximum response of 24.2% for 100ppm NO<SUB>2</SUB> gas concentration at an operating temperature of 200°C with response and recovery times of 13s and 140s, respectively. Finally, the chemisorption mechanism of NO<SUB>2</SUB> gas on the V<SUB>2</SUB>O<SUB>5</SUB> nanorods is discussed.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Effect of solution concentration on physicochemical properties of sprayed V<SUB>2</SUB>O<SUB>5</SUB> nanorods is studied. </LI> <LI> Good re­sponse and short re­sponse- re­covery times of V<SUB>2</SUB>O<SUB>5</SUB> nanorods towards NO<SUB>2</SUB> gas show it is potential material for fab­ri­ca­tion of NO<SUB>2</SUB> sensor. </LI> <LI> The chemisorption mechanism of NO<SUB>2</SUB> gas on the V<SUB>2</SUB>O<SUB>5</SUB> nanorods is discussed. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Dye-sensitized solar cell and electrochemical supercapacitor applications of electrochemically deposited hydrophilic and nanocrystalline tin oxide film electrodes

Mane, R.S.,Chang, J.,Ham, D.,Pawar, B.N.,Ganesh, T.,Cho, B.W.,Lee, J.K.,Han, S.H. Elsevier 2009 Current Applied Physics Vol.9 No.1

The present article demonstrates the use of low-cost electrochemically synthesized hydrophilic and nanocrystalline tin oxide film electrodes at room temperature in dye-sensitized solar cells and electrochemical supercapacitors. A mixed phase of tin instead of single phase composed of uniformly distributed irregular spherical grains in a nanometer regime scale was obtained. Tin oxide film electrode showed efficient photoperformance when subjected to dye-sensitized solar cells. The interfacial and specific capacitances of 118.4μF/cm<SUP>2</SUP> and 43.07F/g, respectively, in 0.1M NaOH electrolyte were confirmed from cyclic-voltammetry measurement.