오패산 마우스에서의 단회투여독성시험

이말순 경산대학교 기초과학연구소 2001 基礎科學 Vol.5 No.1

한의학에서 궤양 치료제로 널리 사용되어지고 있는 오패산 (烏貝散)의 마우스 (ICR mouse) 단회투여독성을 관찰하기 위하여 2000, 1000, 500, 250 및 125 mg/kg의 투여 용량으로 암수 마우스에 단회경구투여하였다. 단회투여 후, 14일 동안 임상증상을 매일 관찰하였으며, 5회 이상 체중의 변화를 관찰하였다. 본 실험의 결과 투여 후 14일 동안 설사 또는 연변을 제외한 이상중상이 관찰되지 않았으며, 체중 역시 정상군과 비교하여 유의한 변화가 관찰되지 않았다. 또한 14일 후 부검 시 중요장기의 이상소견 역시 관찰되지 않아 오패산의 독성이 미약한 것으로 관찰되었으며 LD50은 암수 마우스 모두에서 2000 mg/kg 이상으로 관찰되었다. The single dose toxicity of Opaesan, a herbal drug for duodenal ulcer was evaluated in ICR mice. Opaesan was once administered to both sexes of mice at the dote levels of 2000, 1000, 500, 250 and 125 mg/kg for oral route. After single administration, clinical signs were observed every day for 14 days and body weights were measured 5 times including initial measurement on day 0. When observation period was over, the animals were sacrificed and macroscopic examination of major organs was conducted. Neither significant clinical signs nor death after administration was observed during the observation periods except for soft feces or diarrhea. In addition, no abnormal necropsy findings and changes of body weight were observed at terminal necropsy in both sexes. From these results, it is considered that LD50 of Opasan is over 2000 mg/kg in oral administration in both sexes of mice.

텍실브로모보란-메틸 설피드 착물을 이용한 카르복시산의 알데히드로의 부분 환원

차진순,윤말숙,김진억,오세연 嶺南大學校附設 基礎科學硏究所 1985 基礎科學硏究 Vol.5 No.-

The systematic study on the direct partial reduction of carboxylic acids to the corresponding aldehydes with thexylbromoborane-methyl sulfide under the practical conditions (carbon disulfide, room temperature) has been done and also some representative carboxylic acids have been applied to the partial reduction under the optimal reaction condition. The reaction is quite general and the yields of aldehydes are very excellent, especially almost quantitative in aliphaticcarboxylic acids.

리튬-보란계에 의한 유기화합물의 선택환원

尹末淑,金永植,車震淳 嶺南大學校附設 基礎科學硏究所 1986 基礎科學硏究 Vol.6 No.-

A systematic study on the reaction of the lithium-borane system in tetrahydrofuran with representative organic compounds under standardized conditions (THF, 0℃, H?/compd=4/1) has been carried out in order to characterize the reducing characteristics and entity of this reducing system. Most aldehydes and ketones are reduced rapidly to the alcohol stage. Carboxylic acids and acid chlorides are rapidly reduced to corresponding alcohol stages are reduced only slowly. Nitro compounds are very slowly reduced and sulfur compounds are essentially inert to this reducing system. Consequently, this system possesses both reducing characterictics of borane, an acidic type, and lithium borohydride, a basic type.

담낭 적출술 환자의 통계적 고찰(Ⅱ) : 진주와 그 주변 지역을 중심으로(지역적 특성에 관한 고찰) For Evaluation in Chinju and It's surrounding Area(To District characteristic)

姜末順 진주간호보건전문대학 1986 진주간호보건전문대학 논문집 Vol.9 No.1

칼슘투여가 혈중 지질과 칼슘대사에 미치는 영향

한성철,신말순,전형권 龍仁大學校 1998 용인대학교 논문집 Vol.16 No.-

The study was carried to investigate the effects of a long term calcium upon blood lipid metabolism in athletes, The effects of calcium supplementation and hematological variables which are related to calcium metabolism, Subjects(N=30), who were 30 males of athletes, were randomly assigned to two group(placebo group, N=15, experimental group N= 15. Each subject in experimental group was ingested a tablet containing 500mg of calcium three times a day for 8 weeks. Hematological variable were measured pre and post 2, 4, 6, 8 weeks after the tablets ingestion. The results through the statiscal analysis of blood components, calcium, tatal-cholesterol, HDL-C, LDL-C, Total-cholesterol/HDL-C, in lipid components were as follow; 1) Serum calcium was significantly increased in calcium croup after 2 weeks and maintained through out the entire experimental period(P<0.75), while showed no significant change in placebo group. There were significant differences between two group(calcium croup, placebo group). 2) Total cholesterol was decreased after 2, 4, 6, 8 weeks in Calcium group but there was no significantly decreased in placebo group. 3) LDL-C was significantly decreased in calcium group after 2 weeks and maintained through out the entire experimental period(P<0.05), but there was no significantly decreased in placebo group. There were significant differences between two group(calcium group, placebo group). 4) HDL-C was significantly increased in calcium group after 4 weeks and maintained through out the entire experimental period(P<0.05), while showed no significant change in placebo group. There were significant differences between two group(calcium group, placebo croup). 5) TC/HDL-C was decreased after 2, 4, 6, 8 weeks in calcium group but there was no significantly decreased in placebo croup . There were significant differences between two group (P<0.05).

생후 6 개월령 한국재래산양의 위장관에서 chromogranin A 면역반응세포에 대한 분포 및 출현빈도에 관한 연구

이형식,이말순 慶山大學校 基礎科學硏究所 2000 基礎科學 Vol.4 No.2

생후 6개월령의 한국재래산양 위장관에서 chromogranin A 면역반응세포의 각 부위별(위저부, 유문부, 십이지장, 공장, 회장, 결장 및 직장의 7부위) 분포와 출현빈도를 면역조직화학적 방법으로 관찰한 결과, chromogranin A 면역반응세포는 위장관 전부위에서 관찰되었다. 위장관 부위별 출현빈도는 위저부(126.0/1㎟)에서 가장 높은 출현빈도를 나타내었고, 유문부(125.0/1㎟), 직장(80.0/1㎟), 결장(70.0/1㎟), 공장(54.0/1㎟), 회장(54.0/1㎟) 및 십이지장(47.0/1㎟)의 순으로 관찰되었다. 이들 chromogranin A 면역반응세포들은 주로 위저부, 유문부, 십이지장, 회장 및 결장부위에서는 점막샘에 다수, 점막 상피에 소수 관찰되었으며, 공장과 결장에서는 점막 전체에 산재되어 관찰되었다. The regional distribution and relative frequency of chromogranin A-immunoreactive cells in the gastrointestinal tract of the Korean native goat at 6-month-old were studied with immunohistochemical methods. The digestive tract was divided into seven portions, fundus, pylorus, duodenum, jejunum, ileum, colon and rectum. Chromogranin A-immunoreactive cells were detected throughout the whole gastrointestinal tract and they were situated to the mucosal glands, which were located in the basal parts of digestive tract, with round, oval and/or spindle shape and surface epithelium with spindle shape. These cells were most predominant in fundus and the relative frequencies of the other portions were observed to be in the following order : fundus > pylorus > rectum > colon >> jejunum = ileum > duodenum.

장기간의 지구성 운동이 지질과산화에 미치는 영향

김종달,신말순,전형권 龍仁大學校 體育科學硏究所 1998 體育科學硏究論叢 Vol.8 No.1

The purpose of this study was carried to investiate the effects of a long term on blood lipid peroxidation. To achieve the purpose, a chain of experiments were carried out as the followings. First, sixty three Sprague-Dawley rats were randomly assigned into three group: the control group, the endurance training group, the endurance exhaustive group. 21 rats were exercised by endurance training and 21 rats were training in endurance exhaustive training for 12 weeks, but 21 rats were not exercised at all. Endurance training animals were trained 5 days per weeks, for 12 weeks, running for 1 hour at 20m/minute, 0% grade. Endurance exhaustive training animals were trained 5 days per weeks, for 12 weeks, swimming to physical exhaustion. Second, human subjects were randomly assigned to control and endurance training groups. 7 subjects were exercised by endurance training and 7 subjects were not exercised at all. Endurance training groups were trained 4 days per weeks, for 12 weeks, swimming for 2 hour. The trained and rested animals were sacrified for 48 hours after their last training session and sedentary control animals were sacrified at the same time of the day. In human, after their last training session, which was for 48 hours their blood was taken from antecubital vein for sampling. Following 8 weeks, 12 weeks training period, the rats and human were hematological analyzed by the changes of LPO(lipid peroxide: MDA). Statistical techniques for data analysis were One-way ANOVA training times(before, 8 week, 12 week) and two way repeated measure ANOVA to determine the differences among groups. The 5% level of significance was used as the critical level for acceptance of hypotheses for the study. The analysis of data revealed the following findings. There weren't any significant differences in the LPO among three groups following pre and 8 weeks training, but significantly lower in training groups than in control group 12 weeks training in rats. On the other hand, in human, there were no significant differences among three groups following pre training and 8 weeks training, but significantly lower in training groups following 12 weeks training.

주사전자현미경을 이용한 돌거머리(Erpobdella lineata)의 형태

박상래,이말순 효성여자대학교 부설 한국환경위생연구소 1994 환경위생연구 Vol.4 No.1

한국산 돌거머리(E.lineata)의 형태학적인 미세구조를 밝히기 위하여 주사적자현미경을 사용하였다. 그 결과 해부현미경하에서는 관찰되지 않았던 몸통표면의 크고, 작은 피부구멍과 입주위의 감각모 그리고 두부와 몸통, 흡반상에 감각돌기같은 미세구조들이 존재해 있음을 관찰하였으며, 이러한 새로운 형질들은 앞으로의 분류에 있어서 중요한 Key로 이용되리라 사료된다. In order to observe morphological structure of Erpobdella lineata, the Scanning Electron Microscope was used. As the result, finestructures, such as large and small epithelial lacunae at the skin surface, sensilla around the mouth, sensory pappillae on the cephalic portion and the trunk, as well as the suckers were investigated. But under the stereoscopic microscope, those were not visualized in detail.

장애,비장애인 휠체어 농구선수의 유산소 능력

김성수,이동희,황부근,신말순,배종진,윤진환,지용석,안희구 한국특수체육학회 2002 한국특수체육학회지 Vol.10 No.2

The purpose of this study was to analyzed the aerobic capacity of disability· able-bodied wheelchair basketball players. Cardiorespiratory parameters were measured during graded maximal exercise test. Exercise time, HRpeak, oxygen uptake(VO_2, L·min^-1, ml·min^-1·kg^-1), carbon dioxide production(VCO_2, L·min^-1), ventilation(L·min^-1) were examined in disability national elite male wheelchair basketball at letes(DA, N = 10), able-bodied wheelchair basketball athletes(AA, N = 10). All data were expressed in means and standard deviation. Exercise time, HRpeak, oxygen uptake, carbon dioxide production, ventilation were not different between groups. In conclusion, aerobic capacity of disability national elite male wheelchair basketball athletes is the same able-bodied wheelchair basketball athletes. These finding suggest that chronic wheelchair basketball training produce local adaptations in the functional upper body musculature.

피조개 발의 점액질에 대한 조직화학적 연구

최병태,조기진,홍말숙,정권순,조운복 釜山大學校 師範大學 1995 교사교육연구 Vol.31 No.-

피조개 발의 조직학적 구조는 hematoxylin-eosin염색 및 Weigert iron hematoxylin염색을, 점액질 성상을 검색하기 위하여 PAS반응, diastase 침지 후 PAS반응, alcian blue pH 2.5염색, alcian blue pH 1.0염색, alcian blue pH 2.5-PAS염색, alcian blue pH 1.0-PAS염색, aldehyde fuchsin pH 1.7-alcian blue pH 2.5염색 및 0.25% toluidine blue염색을 실시하였다. 피조개의 발은 상피, 상피하결합조직 및 근층으로 이루어져 있었으며 상피는 단층섬모원주세포로 되어 있었다. 상피하결합조직 및 근층에 다세포성 점액선인 발선이 발달되어 있었고 도관은 결합조직과 상피세포를 거쳐 개구되어 있었다. 근층은 종주근, 수직근 및 횡근섬유로 구성되어 있었다. 점액질의 조성을 보면 상피세포섬모에는 sialomucin만을, 상피세포는 중성점액질만 함유하고 있었다. 상피세포 및 결합조직내 점액도관에는 산성과 중성점액질의 혼합성인 것과 산성점액질만 함유한 것이 혼재하나 전자가 더 많았다. 산성점액질의 성상으로 보아 대부분이 강 sulfomucin을 가지며 일부는 약 sulfomucin과 sialomucin의 혼합성이었다. 대부분 발선의 점액선포에 대한 점액질 조성은 점액도관과 유사하였으나 일부 상부발선의 점액선포는 중성점액질만을, 하부발선의 점액선포는 sialomucin만을 함유하였다. This study was performed to investigate the histological structure and the histochemical properties of the mucosubstances in the foot of Anadara broughonii. For the histological structure, hematoxylin-eosin and Weigert iron hematoxylin staining were used. The mucosubstances stained with PAS, PAS-diastase, 0.25% toluidine blue, alcian blue pH 2.5, alcian blue pH 1.0, alcian blue pH 2.5-PAS, alcian blue pH 1.0-PAS, aldehyde fuchsin pH 1.7-alcian blue pH 2.5 methods. The foot was composed basically of three layers: epithelium, subepithelial connective tissue and muscular layers. The foot was lined by simple ciliated columnar epithelial cell. Foot glands consisted of multicelluear mucous acini were present in the subepithelial connective tissue and muscular layer. The mucous duct of foot glands was distributed from connective tissue to epithelial cells, and open onto the surface of the foot. The foot muscle traveled in three planes as longitudinal, transverse and vertical muscle fiber. About the properties of mucosubstances on the foot, cilia of epithelial cell contained sialomucin only and epithelial cell neutral mucosubstances only. Most of mucous duct within epithelial cells and connective tissue showed a mixture of acidic and neutral mucosubstances, while a few mucous ducts were contained acidic ones only. Among acidic mucosubstances, notable strong sulfomucin were found in most mucous cell of duct, and a mixture of weak sulfomucin and sialomucin appeared in a few cells. The properties of mucosubstances in the food glands were similiar to those of mucous duct. But a few mucous acini contained neutral mucosubstances and sialomucin only in upper and lower portion of foot gland, respectively.