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Mahe Talat 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.4
Urease was used for estimation of Cu²+ in aqueous solution. Process variables were optimized by Central Composite Design using MINITAB® 15 software. Results predicted by the design were close to experimental value indicating suitability of the model. 3D surface plot and isoresponse contour plot were helpful in predicting results by performing limited set of experiments. Urease was extracted from discarded seeds of pumpkin to apparent homogeneity by heat fractionation and gel filtration. Homogeneous enzyme preparation was further immobilized in 3.5% alginate. Effect of Cu²+ on the activity of soluble and immobilized enzyme was investigated. Enzyme inhibition was biphasic, irreversible, and non-competitive (Ki = 1.06 μM).
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Om Prakash,S. H. Hasan,Rajesh K. Pandey,Mahe Talat 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.2
Enzyme urease is extracted from the discarded seeds of pumpkin. Urease was purified to apparent homogeneity (5.2 fold) by heat treatment at 48 ± 1℃ and gel filtration through Sephadex G-200. Effect of model metal ions on the activity of the homogeneous enzyme preparation (sp. activity 353 U/mg protein, A280/A260 = 1.12) of soluble as well as immobilized enzyme was investigated. The soluble and immobilized urease has been used for the quantitative estimation of general water pollution with heavy metal ions like Hg²+, Cu²+, Cd²+, and Co²+. The measurements of the urease residual activity have been carried out in tris-acetate buffer after pre-incubation of model metal salt. The inhibition was found to be biphasic with an initial rapid loss of activity and remainder in slow phase of 10~15 min. The immobilization was done in 3.5% alginate beads leading to 86% of entrapment. There was no leaching of the enzyme over a period of 15 days at 4℃. The beads were fairly stable up to 50℃ and exhibited activity even at -10℃. The inhibition by these ions was non-competitive and irreversible, hence could not be restored by dialysis. Based on the values of inhibition constant Ki the heavy-metal ions were found to inhibit urease in the following order Hg²+ > Cu²+ > Cd²+ > Co²+.
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Belabbas Dihia,Koch Caroline,Chaudru Ségolène,Lederlin Mathieu,Laviolle Bruno,Le Pabic Estelle,Boulmier Dominique,Heautot Jean-François,Mahe Guillaume 대한영상의학회 2020 Korean Journal of Radiology Vol.21 No.11
Objective: We aimed to assess the effects of remote ischemic pre-conditioning (RIPC) on the incidence of contrast-induced nephropathy (CIN) after an intravenous (IV) or intra-arterial injection of contrast medium (CM) in patient and control groups. Materials and Methods: This prospective, randomized, single-blinded, controlled trial included 26 patients who were hospitalized for the evaluation of the feasibility of transcatheter aortic valve implantation and underwent investigations including contrast-enhanced computed tomography (CT), with Mehran risk scores greater than or equal to six. All the patients underwent four cycles of five minute-blood pressure cuff inflation followed by five minutes of total deflation. In the RIPC group (n = 13), the cuff was inflated to 50 mm Hg above the patient’s systolic blood pressure (SBP); in the control group (n = 13), it was inflated to 10 mm Hg below the patient’s SBP. The primary endpoint was the occurrence of CIN. Additionally, variation in the serum levels of cystatin C was assessed. Results: One case of CIN was observed in the control group, whereas no cases were detected in the RIPC group (p = 0.48, analysis of 25 patients). Mean creatinine values at the baseline, 24 hours after injection of CM, and 48 hours after injection of CM were 88 ± 32 μmol/L, 91 ± 28 μmol/L and 82 ± 29 μmol/L, respectively (p = 0.73) in the RIPC group, whereas in the control group, they were 100 ± 36 μmol/L, 110 ± 36 μmol/L, and 105 ± 34 μmol/L, respectively (p = 0.78). Cystatin C values (median [Q1, Q3]) at the baseline, 24 hours after injection of CM, and 48 hours after injection of CM were 1.10 [1.08, 1.18] mg/L, 1.17 [0.97, 1.35] mg/L, and 1.12 [0.99, 1.24] mg/L, respectively (p = 0.88) in the RIPC group, whereas they were 1.11 [0.97, 1.28] mg/L, 1.13 [1.08, 1.25] mg/L, and 1.16 [1.03, 1.31] mg/L, respectively (p = 0.93), in the control group. Conclusion: The risk of CIN after an IV injection of CM is very low in patients with Mehran risk score greater than or equal to six and even in the patients who are unable to receive preventive hyperhydration. Hence, the Mehran risk score may not be an appropriate method for the estimation of the risk of CIN after IV CM injection.
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