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Improved Method for the Preparation of Silk Fibroin Hydrolysates
Madyarov, Shukhrat,Lee, Kwang Gill,Yeo, Joo Hong,Nam, Jin,Lee, Yong Woo 한국잠사학회 1999 한국잠사곤충학회지 Vol.41 No.2
An improvement of methods in fibroin hydrolysates preparation significantly enlarges their applications to practical use. Acidic hydrolysis by hydrochloric acid is one of the methods for silk fibroin depolymerization. A low yield of final product and long time of the process are the demerits of this method. Possibility of preparation of water-soluble silk hydrolysates with more yield and less expenses is investigated in this study. Such possibility is occurred with the increasing treatment temperature and simultaneously decreasing treatment time, concentration of hydrochloric acid and respectively, the concentration of sodium hydroxide used for neutralization of hydrolysates after hydrolysis. Colour is decreased in this case and a small amount of activated carbon is demanded for decoluorization of hydrolysates. Production cost of desalted products are reduced, too. Protection of hydrolysates against precipitation after neutralization, and separation and during concentrating process is the other merit of this method. Creamy-coloured insoluble silk powder is the remainder of hydrolyzed fibroin. This is the only defect of the proposed method. However, this insoluble powder can be used as a matrix for the irnmobilization of enzymes and other physiological active substances. Fine particles of this powder can be used as additives for artificial diets and cultural media, as well as raw materials for polymer membranes, etc.
Madyarov, S.R.,Mirzaeva, G.S.,Otarbaev, D.O.,Khamidi, K.S.,Kamilova, S.I.,Akhmerov, R.N.,Khamraev, A.S. Korean Society of Sericultural Science 2003 International Journal of Industrial Entomology Vol.7 No.2
The silkworm Bombyx mori L. was studied as the potential “host” of popular in Uzbekistan biocontrol ectoparasite, entomophagous Bracon hebetor Say. Being one of representatives of economic-beneficial insects, the silkworm (larvae, pupae and imago) can be used as highly sensitive test organism for revealing of neuro toxic effects of insects venom as well as of their purified components in screening assays. In comparative aspect, except a mulberry silkworm, representatives of Uzbekistan pests cotton-boll worm Helycoverpa armigera Hbn., lesser mulberry pyralid Glyphodes pyloalis Wlk., codling moth Corpocapsa pomonella L., malaria mosquito Anopheles claviger and Colorado potato beetle Leptinotarsa decemlineata Say have been subjected to insect toxic test of bracon venom gland extract (VGE) and its fractions which were obtained by gel-chromatography on Sephadex G-100. The paralyzing effect of the VGE and its fractions was shown in a various degree on the pests.
Biotechnological Approaches in Sericultural Science and Technology of Uzbekistan
Madyarov Shukhrat R. Korean Society of Sericultural Science 2005 International Journal of Industrial Entomology Vol.11 No.1
Biotechnological researches in Central Asian Research Institute of Sericulture were developed since 1981 when laboratory of artificial diets for mulberry silkworm was created. The researches were directed not only to creation of accessible artificial diets (AD) for off-season mass rearing of economical beneficial insect and for industrialization of sericulture but also to obtaining of easy reproducible source of animal proteins, carbohydrates, lipids, enzymes, enzymes inhibitors and other metabolites - bioresource for manufacture of oriental medicine preparations. Space experiments with silkworms became possible with use of AD. These experiments used mulberry silkworm as high effective board test organism with outstanding developmental, reproductive and transgenic properties having important significance in future of cosmonautics. Introduction to practice of bioprotective and ecologically pure method of drying and sterilizing of green cocoons and by-products of silk manufacture allows to increase efficiency of cocoons reeling technology as well as to preserve nativity of biological active substances in mulberry silkworm pupae that is unachievable by traditional methods of raw material processing. New spheres of application of two important silk proteins - fibroin and sericin in medicine, cosmetics, for bio- and nanotechnology are opening with use of biotechnological approaches. At present the achievements and experience of R & D in these fields are used in creation of modern pests biocontrol agents. Schemes of efficient processing of cocoon raw material, by-products and wastes of sericulture and cocoon processing are devel- Institute of Physiology and Biophysics, Uzbek Academy of Sciences, I, Niyazov street, 700095, Tashkent, Uzbekistan. oped for obtaining of traditional and new consumer products.
Review : Biotechnological Approaches in Sericultural Science and Technology of Uzbekistan
( Shukhrat R. Madyarov ) 한국잠사학회 2005 International Journal of Industrial Entomology Vol.11 No.1
Biotechnological researches in Central Asian Research Institute of Sericulture were developed since 1981 when laboratory of artificial diets for mulberry silk-worm was created. The researches were directed not only to creation of accessible artificial diets (AD) for off-season mass rearing of economical beneficial insect and for industrialization of sericulture but also to obtaining of easy reproducible source of animal proteins, carbohydrates, lipids, enzymes, enzymes inhibitors and other metabolites-bioresource for manufacture of oriental medicine preparations. Space experiments with silkworms became possible with use of AD. These experiments used mulberry silkworm as high effective board test organism with outstanding developmental, reproductive and transgenic properties having important significance in future of cosmonautics. Introduction to practice of bioprotective and ecologically pure method of drying and sterilizing of green cocoons and by-products of silk manufacture allows to increase efficiency of cocoons reeling technology as well as to preserve nativity of biological active substances in mulberry silkworm pupae that is unachievable by traditional methods of raw material processing. New spheres of application of two important silk proteins-fibroin and sericin in medicine, cosmetics, for bio- and nanotechnology are opening with use of biotechnological approaches. At present the achievements and experience of R&D in these fields are used in creation of modern pests biocontrol agents. Schemes of efficient processing of cocoon raw material, by-products and wastes of sericulture and cocoon processing are developed for obtaining of traditional and new consumer products.