Enzyme-Linked, Biotin-Streptavidin Bacterial-Adhesion Assay for Helicobacter pylori Lectin-Like Interactions with Cultured Cells

MURILLO, GUZMAN,ANTONIA, MARIA,ASCENCIO, FELIPE 한국미생물 · 생명공학회 2001 Journal of microbiology and biotechnology Vol.11 No.1

A simple method for studying the lectin-like interactions between Helicobacter pylori and cultured human epithelial cell lines was developed using an enzyme-linked, biotin-streptavidin bacterial-adhesion assay. The present study suggests that this method is suitable for evaluating the participation of lectin interactions in the adhesion of H. pylori to cultured HeLa S3 and Kato Ⅲ cells, both fixed and glycosidase-treated cells, as well as assessing glycoconjugated binding inhibition studies. The time-course and dose-dependent kinetics of the biotin-labeled H. pylori adhesion to the formaldehyde-fixed Hela S3 and Kato Ⅲ cell lines exhibited saturation. In addition, the binding of the biotin-labeled H. pylori to the formaldehyde-fixed cultured cells was partially blocked by pre-incubation with glycoconjugates and polyclonal antibodies against a heparan sulfate binding protein from H. pylori.

Improving Morphological Quality and Uniformity of Hydrothermally Grown ZnO Nanowires by Surface Activation of Catalyst Layer

Murillo, Gonzalo,Lozano, Helena,Cases-Utrera, Joana,Lee, Minbaek,Esteve, Jaume Springer US 2017 NANOSCALE RESEARCH LETTERS Vol.12 No.1

<P>This paper presents a study about the dependence of the hydrothermal growth of ZnO nanowires (NWs) with the passivation level of the active surface of the Au catalyst layer. The hydrothermal method has many potential applications because of its low processing temperature, feasibility, and low cost. However, when a gold thin film is utilized as the seed material, the grown NWs often lack morphological homogeneity; their distribution is not uniform and the reproducibility of the growth is low. We hypothesize that the state or condition of the active surface of the Au catalyst layer has a critical effect on the uniformity of the NWs. Inspired by traditional electrochemistry experiments, in which Au electrodes are typically activated before the measurements, we demonstrate that such activation is a simple way to effectively assist and enhance NW growth. In addition, several cleaning processes are examined to find one that yields NWs with optimal quality, density, and vertical alignment. We find cyclic voltammetry measurements to be a reliable indicator of the seed-layer quality for subsequent NW growth. Therefore, we propose the use of this technique as a standard procedure prior to the hydrothermal synthesis of ZnO NWs to control the growth reproducibility and to allow high-yield wafer-level processing.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1186/s11671-017-1838-x) contains supplementary material, which is available to authorized users.</P>

Chemical Composition, In vitro Gas Production, Ruminal Fermentation and Degradation Patterns of Diets by Grazing Steers in Native Range of North Mexico

Murillo, M.,Herrera, E.,Carrete, F.O.,Ruiz, O.,Serrato, J.S. Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.10

The objective of the study was to quantify annual and seasonal differences in the chemical composition, in vitro gas production, in situ degradability and ruminal fermentation of grazing steers… diets. Diet samples were collected with four esophageal cannulated steers ($350{\pm}3$ kg BW); and four ruminally cannulated heifers ($342{\pm}1.5$ kg BW) were used to study the dry matter degradation and fermentation in rumen. Data were analyzed with repeated measurements split plot design. The crude protein, in vitro dry matter digestibility and metabolizable energy were higher during the first year of trial and in the summer (p<0.01). The values of calcium, phosphorus, magnesium, zinc and copper were higher in summer (p<0.05). The gas produced by the soluble and insoluble fractions, as well as the constant rate of gas production were greater in summer and fall (p<0.01). The ammonia nitrogen ($NH_3N$) and total volatile fatty acids concentrations in rumen, the soluble and degradable fractions, the constant rate of degradation and the effective degradability of DM and NDF were affected by year (p<0.05) and season (p<0.01). Our study provides new and useful knowledge for the formulation of protein, energetic and mineral supplements that grazing cattle need to improve their productive and reproductive performance.

Effect of Supplemental Corn Dried Distillers Grains with Solubles Fed to Beef Steers Grazing Native Rangeland during the Forage Dormant Season

Murillo, M.,Herrera, E.,Ruiz, O.,Reyes, O.,Carrete, F.O.,Gutierrez, H. Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.5

Two experiments were conducted to evaluate the effects of the level of corn dry distillers grains with solubles (CDDGS) supplementation on growing performance, blood metabolites, digestion characteristics and ruminal fermentation patterns in steers grazing dormant forage. In Exp. 1, of growth performance, 120 steers ($204{\pm}5kg$ initial body weight [BW]) were distributed randomly into 3 groups (each of 40 steers), which were provided with the following levels of CDDGS supplement: 0%, 0.25%, or 0.50% BW. All groups of steers were grazed for 30 days in each of 3 grazing periods (March, April, and May). Approximately 1,000 ha of the land was divided with electric fencing into 3 equally sized pastures (333 ha in size). Blood samples were collected monthly from 20 steers in each grazing group for analysis of glucose (G), urea-nitrogen (UN) and non-esterified fatty acids. Final BW, average daily gain (ADG) and supplement conversion (CDDGS-C) increased with increasing levels of CDDGS supplementation (p<0.05).The CDDGS supplementation also increased the plasma G and UN concentrations (p<0.05). In Exp. 2, of digestive metabolism, 9 ruminally cannulated steers ($BW=350{\pm}3kg$) were distributed, following a completely randomized design, into groups of three in each pasture. The ruminally cannulated steers were provided the same levels of CDDGS supplementation as in the growing performance study (0%, 0.25%, and 0.50% BW), and they grazed along with the other 40 steers throughout the grazing periods. The dry matter intake, crude protein intake, neutral detergent fiber intake (NDFI), apparent digestibility of dry matter (ADDM), crude protein (ADCP) and neutral detergent fiber (ADNDF) increased with increasing levels of CDDGS supplementation (p<0.05). The ruminal degradation rates of CP (kdCP), NDF (kdNDF) and passage rate (kp) also increased with increasing levels of CDDGS supplementation (p<0.05). Ruminal ammonia nitrogen ($NH_3$-N) and propionate concentrations also increased with increasing levels of CDDGS supplementation (p<0.05). However, acetate concentrations decreased with increasing levels of CDDGS supplementation (p<0.05). Liquid dilution rate increased with increasing levels of CDDGS supplementation but ruminal liquid volume decreased (p<0.05). On the basis of these findings, we can conclude that CDDGS supplementation enhanced the productive performance of cattle grazing native rangeland without negatively affecting forage intake, glucose and urea-nitrogen blood concentrations, ruminal degradation and ruminal fermentation patterns.

1D AND 2D MODELS OF SOLUTE TRANSPORT USING CONSERVATIVE SEMI-LAGRANGIAN TECHNIQUES

MURILLO JAVIER,BURGUETE JAVIER,BRUFAU PILAR,GARCIA-NAVARRO PILAR 한국수자원학회 2005 한국수자원학회 학술대회 Vol.2005 No.1

The Role of Business Ethics in Business Schools: A European Perspective

David Murillo Bonvehí 한국윤리경영학회 2012 한국윤리경영학회 학술대회 발표논문집 Vol.2012 No.3

This brief paper presents a personal perspective on the role of business ethics in business schools. The discussion is divided into four phases: a) I start by presenting the most evident proofs of the current maladies in management studies; b) I then briefly discuss the institutional context of our role as scholars in business schools; c) then with the assistance of the sociology of knowledge, I give a view on the meaning of working from within the existing management paradigm; and d) finally, I present what I consider to be the current role of business ethics; namely, encouraging the institutional and academic transformation of management narratives.

El tratamiento mortuorio del cuerpo humano en las antiguas poblaciones mexicanas

Silvia Murillo Rodriguez 부산외국어대학교 중남미지역원 2013 이베로아메리카 Vol.15 No.2

X-ray and gamma ray shielding behavior of concrete blocks

Hernandez-Murillo Christian Geovanni,Molina Contreras J. Rafael,Escalera-Velasco Luis Alberto,de Leon-Martínez Héctor Asael,Rodriguez-Rodriguez José Antonio,Vega-Carrillo Héctor Rene 한국원자력학회 2020 Nuclear Engineering and Technology Vol.52 No.8

The shielding characteristics of two concrete blocks, widely used in the building industry in Mexico have been determined. These characteristics include the mass interaction coefficients, the linear attenuation coefficients and the half-value layers. The energy-dispersed X-ray fluorescence shows that the percentage mass content of each atom in the sample, and the atomic volume of the constituent elements of a material, plays an important role in its shielding capabilities. The total linear attenuation coefficients and the half-value layers were analyzed for a set of photon energies related to X-rays for diagnosis and cancer treatment with linear accelerators. Our results show that the concrete blocks have similar photon attenuation coefficients than the Portland concrete and better features than gypsum

Performance Tests of Geotextile Permeability for Tunnel Drainage Systems

C. A. Murillo,신종호,김강현,J. E. Colmenares 대한토목학회 2014 KSCE JOURNAL OF CIVIL ENGINEERING Vol.18 No.3

In double-lined tunnels, geotextiles are installed between shotcrete and concrete linings to drain ground water that is in thecircumferential boundary of the tunnels. During the concrete lining placement, the geotextile often experiences pressures from youngconcrete on the curved and rough shotcrete surfaces. The pressures are transferred through a waterproof membrane, and act in thenormal direction to the curved shotcrete face. The geotextile flow behavior under these conditions cannot be represented by standardgeotextile permeability tests. Instead, it requires specially designed performance tests that consider field conditions. A new device toevaluate the permeability of the geotextile in pressurized curved flow channels is proposed; it adopts a flexible loading system and acurved and rough model plate. Testing of geotextiles used for tunnel drainage systems, using the proposed arrangement, shows thatthe effects of the tortuousity of the flow in pressurized channels affects significantly the geotextile permeability.

Carbazole-Based Hyperbranched Polyester Polyol: Structural, Rheological, Thermal and Optical Properties

Edwin A. Murillo,Margarita Cerón,M. Judith Percino 한국고분자학회 2021 Macromolecular Research Vol.29 No.4

The present study sought to obtain a hyperbranched polyester polyol derivative with fluorescent properties. Initially, a 4-((4-(9H-carbazol-9-yl)benzyl)oxy)- 4-oxobut-2-enoic acid (CAV) was synthesized and esterified with a hyperbranched polyester polyol obtained from fourth generation (HBP) to synthesize carbazolebased hyperbranched polyester polyols (HBPCAV). The proportions employed of CAV were 5, 10, 15, and 20 wt% relative to that of HBP. The grafted CAV percentage (P gCAV) increased with the CAV content. The conversion percentages (CP) of the reactions for obtaining HBPCAV were higher than 95% and the hydroxyl values (OHV) were lower than that of HBP. The modification percentage (MP) of the HBP was between 9.33 and 27.38%. Using proton nuclear magnetic resonance (1H NMR) and infrared (IR) analyses was evidenced the formation of CAV and HBPCAV. Dynamic light scattering (DLS) analysis showed that HBPCAV exhibited aggregations. The number average molar mass (M n), viscosity (η), and glass transition temperature (T g) values of the HBPCAV samples were higher than those of the HBP, and also increased with the P gCAV values. The rheological behaviors of the HBP and HBPCAV samples were mainly Newtonian and shear-thinning, respectively. All HBPCAV exhibited electroluminescent properties.