Search for a very light NMSSM Higgs boson produced in decays of the 125 GeV scalar boson and decaying into τ leptons in pp collisions at s = 8 $$ \sqrt{s}=8 $$ TeV

Khachatryan, V.,Sirunyan, A. M.,Tumasyan, A.,Adam, W.,Asilar, E.,Bergauer, T.,Brandstetter, J.,Brondolin, E.,Dragicevic, M.,Erö,, J.,Flechl, M.,Friedl, M.,Frü,hwirth, R.,Ghete, V. M.,Hartl, C. Springer-Verlag 2016 Journal of high energy physics Vol.2016 No.1

<P>A search for a very light Higgs boson decaying into a pair of tau leptons is presented within the framework of the next-to-minimal supersymmetric standard model. This search is based on a data set corresponding to an integrated luminosity of 19.7 fb(-1) of proton-proton collisions collected by the CMS experiment at a centre-of-mass energy of 8 TeV. The signal is defined by the production of either of the two lightest scalars, h(1) or h(2), via gluon-gluon fusion and subsequent decay into a pair of the lightest Higgs bosons, a(1) or h(1). The h(1) or h(2) boson is identified with the observed state at a mass of 125 GeV. The analysis searches for decays of the a(1) (h(1)) states into pairs of tau leptons and covers a mass range for the a(1) (h(1)) boson of 4 to 8 GeV. The search reveals no significant excess in data above standard model background expectations, and an upper limit is set on the signal production cross section times branching fraction as a function of the a(1) (h(1)) boson mass. The 95% confidence level limit ranges from 4.5 pb at m(a1) (m(h1)) = 8 GeV to 10.3 pb at m(a1) (m(h1)) = 5 GeV.</P>

Regulation of cancer cell death by a novel compound, C604, in a c-Myc-overexpressing cellular environment

Jo, M.J.,Paek, A.R.,Choi, J.S.,Ok, C.Y.,Jeong, K.C.,Lim, J.H.,Kim, S.H.,You, H.J. North-Holland ; Elsevier Science Ltd 2015 european journal of pharmacology Vol.769 No.-

<P>The proto-oncogene c-Myc has been implicated in a variety of cellular processes, such as proliferation, differentiation and apoptosis. Several c-Myc targets have been studied; however, selective regulation of c-Myc is not easy in cancer cells. Herein, we attempt to identify chemical compounds that induce cell death in c-Myc-overexpressing cells (STF-cMyc and STF-Control) by conducting MTS assays on approximately 4000 chemical compounds. One compound, C604, induced cell death in STF-cMyc cells but not STF-Control cells. Apoptotic proteins, including caspase-3 and poly(ADP-ribose) polymerase (PAPP), were cleaved in C604-treated STF-cMyc cells. In addition, 5W620, HCT116 and NCI-H23 cells, which exhibit higher basal levels of c-Myc, underwent apoptotic cell death in response to C604, suggesting a role for C604 as an inducer of apoptosis in cancer cells with c-Myc amplification. C604 induced cell cycle arrest at the G2/M phase in cells, which was not affected by apoptotic inhibitors. Interestingly, C604 induced accumulation of c-Myc and Cdc25A proteins. In summary, a chemical compound was identified that may induce cell death in cancer cells with c-Myc amplification specifically through an apoptotic pathway. (C) 2015 Elsevier B.V. All rights reserved.</P>

A C1 inhibitor ortholog from rock bream (Oplegnathus fasciatus): Molecular perspectives of a central regulator in terms of its genomic arrangement, transcriptional profiles and anti-protease activities of recombinant peptide

Umasuthan, N.,Bathige, S.D.N.K.,Revathy, K.S.,Wickramaarachchi, W.D.N.,Wan, Q.,Whang, I.,Kim, E.,Park, M.A.,Park, H.C.,Lee, J. Pergamon Press ; Elsevier Science 2014 DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Vol.42 No.2

C1 inhibitor (C1Inh), a member of serpin superfamily, is a crucial regulator of the activation of various plasmatic cascades associated with immunity and inflammation. This study describes the identification and characterization of a C1Inh gene from rock bream Oplegnathus fasciatus (OfC1Inh) at structural, expressional and functional levels. The cDNA-(2245bp) and corresponding gDNA-sequences (5.2kbp) of OfC1Inh were isolated from rock bream transcriptome- and BAC-libraries, respectively. Predicted amino acid sequence of OfC1Inh revealed a two-domain architecture composed of an N-terminal region with two Ig-like domains and a C-terminal region with a serpin domain. Tertiary model of OfC1Inh disclosed its active site topology. In the multi-exonic genomic arrangement of OfC1Inh, it consisted of eleven exons disjoined by ten introns as observed in few other fish homologs. Our comparative analysis indicated that the teleostean C1Inhs were distinct from their non-teleostean vertebrate counterparts in terms of their (1) extended N-terminal domains, (2) evolutionary divergence and (3) exon-intron distribution. The OfC1Inh had a TATA-deficient promoter with a putative initiator element, and two tandemly arranged downstream promoter elements. Several components associated with the immune and inflammatory transcriptional activation were also predicted to exist in 5' flanking region of OfC1Inh. The exclusive mRNA levels in liver and moderate levels in extra-hepatic tissues intimated the diversified importance of OfC1Inh in rock bream physiology. We also provide an evidence for the involvement of OfC1Inh in immune balance, based on its modulated transcription upon different PAMP (lipopolysaccharide and poly I:C)- or pathogen (Streptococcus iniae and rock bream irido virus)-challenges. A recombinantly expressed fusion protein [(r)OfC1Inh] was employed in demonstrating the anti-protease function of OfC1Inh. The (r)OfC1Inh exhibited detectable inhibitory activity against C1 esterase and thrombin, where the anti-C1 esterase role was shown to be potentiated by heparin. Taken together, the results of this study provide the first line of evidence for the possible involvement of a teleostean C1Inh in fish immunity, based on its expressional response(s) and inhibitory properties against two enzymes involved in biological cascades.

A SUB-SATURN MASS PLANET, MOA-2009-BLG-319Lb

Miyake, N.,Sumi, T.,Dong, Subo,Street, R.,Mancini, L.,Gould, A.,Bennett, D. P.,Tsapras, Y.,Yee, J. C.,Albrow, M. D.,Bond, I. A.,Fouqué,, P.,Browne, P.,Han, C.,Snodgrass, C.,Finet, F.,Furusawa, K IOP Publishing 2011 The Astrophysical journal Vol.728 No.2

<P>We report the gravitational microlensing discovery of a sub-Saturn mass planet, MOA-2009-BLG-319Lb, orbiting a K-or M-dwarf star in the inner Galactic disk or Galactic bulge. The high-cadence observations of the MOA-II survey discovered this microlensing event and enabled its identification as a high-magnification event approximately 24 hr prior to peak magnification. As a result, the planetary signal at the peak of this light curve was observed by 20 different telescopes, which is the largest number of telescopes to contribute to a planetary discovery to date. The microlensing model for this event indicates a planet-star mass ratio of q = (3.95 +/- 0.02) x 10(-4) and a separation of d = 0.97537 +/- 0.00007 in units of the Einstein radius. A Bayesian analysis based on the measured Einstein radius crossing time, t(E), and angular Einstein radius,theta(E), along with a standard Galactic model indicates a host star mass of M-L = 0.38(-0.18)(+0.34) M-circle dot and a planet mass of M-p = 50(-24)(+44)M(circle plus), which is half the mass of Saturn. This analysis also yields a planet-star three-dimensional separation of a = 2.4(-0.6)(+1.2) AU and a distance to the planetary system of D-L = 6.1(-1.2)(+1.1) kpc. This separation is similar to 2 times the distance of the snow line, a separation similar to most of the other planets discovered by microlensing.</P>

Measurement of the top quark mass in the dileptonic tt¯ decay channel using the mass observables Mbℓ , MT2 , and Mbℓν in pp collisions at s=8 TeV

Sirunyan, A. M.,Tumasyan, A.,Adam, W.,Asilar, E.,Bergauer, T.,Brandstetter, J.,Brondolin, E.,Dragicevic, M.,Erö,, J.,Flechl, M.,Friedl, M.,Frü,hwirth, R.,Ghete, V. M.,Hartl, C.,Hö,rmann, N American Physical Society 2017 Physical Review D Vol.96 No.3

<P>A measurement of the top quark mass (M-t) in the dileptonic t (t) over bar decay channel is performed using data from proton-proton collisions at a center-of-mass energy of 8 TeV. The data was recorded by the CMS experiment at the LHC and corresponds to an integrated luminosity of 19.7 +/- 0.5 fb(-1). Events are selected with two oppositely charged leptons (l = e, mu) and two jets identified as originating from b quarks. The analysis is based on three kinematic observables whose distributions are sensitive to the value of Mt. An invariant mass observable, M-bl, and a 'stransverse mass' observable, M-T2, are employed in a simultaneous fit to determine the value of M-t and an overall jet energy scale factor (JSF). A complementary approach is used to construct an invariant mass observable, M-blv, that is combined with M-T2 to measure M-t. The shapes of the observables, along with their evolutions in M-t and JSF, are modeled by a nonparametric Gaussian process regression technique. The sensitivity of the observables to the value of M-t is investigated using a Fisher information density method. The top quark mass is measured to be 172.22 +/- 0.18(stat)(-0.93)(+0.89) (syst) GeV.</P>

Pharmacokinetics and Mammary Residual Depletion of Erythromycin in Healthy Lactating Ewes

Goudah, A.,Sher Shah, S.,Shin, H.C.,Shim, J.H.,Abd El-Aty, A. M. Blackwell Publishing Ltd 2007 Journal of veterinary medicine. A, Physiology, pat Vol.54 No.10

<P>Summary</P><P>The aim of this investigation was to examine the pharmacokinetics and mammary excretion of erythromycin administered to lactating ewes (<I>n</I> = 6) by the intravenous (i.v.), intramuscular (i.m.) and subcutaneous (s.c.) routes at a dosage of 10 mg/kg. Blood and milk samples were collected at pre-determined times, and a microbiological assay method was used to measure erythromycin concentrations in serum and milk. The concentration–time data were analysed by compartmental and non-compartmental kinetic methods. The serum concentration–time data of erythromycin were fit to a two-compartment model after i.v. administration and a one-compartment model with first-order absorption after i.m. and s.c. administration. The elimination half-life (<I>t</I><SUB>1/2&bgr;</SUB>) was 4.502 ± 1.487 h after i.v. administration, 4.874 ± 0.296 h after i.m. administration and 6.536 ± 0.151 h after s.c. administration. The clearance value (Cl<SUB>tot</SUB>) after i.v. dosing was 1.292 ± 0.121 l/h/kg. After i.m. and s.c. administration, observed peak erthyromycin concentrations (<I>C</I><SUB>max</SUB>) of 0.918 ± 0.092 <I>&mgr;</I>g/ml and 0.787 ± 0.010 <I>&mgr;</I>g/ml were achieved at 0.75 and 1.0 h (<I>T</I><SUB>max</SUB>) respectively. The bioavailability obtained after i.m. and s.c. administration was 91.178 ± 10.232% and 104.573 ± 9.028% respectively. Erythromycin penetration from blood to milk was quick for all the routes of administration, and the high AUC<SUB>milk</SUB>/AUC<SUB>serum</SUB> (1.186, 1.057 and 1.108) and C<SUB>max‐milk</SUB>/C<SUB>max‐serum</SUB> ratios reached following i.v., i.m. and s.c. administration, respectively, indicated an extensive penetration of erythromycin into the milk.</P>

Polyphasic delimitation of a filamentous marine genus, Capillus gen. nov. (Cyanobacteria, Oscillatoriaceae) with the description of two Brazilian species

Taiara A. Caires,Goia de M. Lyra,Guilherme S. Hentschke,Aaron Matheus S. da Silva,Valter L. de Araújo,Célia L. Sant’Anna,José Marcos de C. Nunes 한국조류학회I 2018 ALGAE Vol.33 No.4

Lyngbya C. Agardh ex Gomont is a nonheterocytous cyanobacterial genus whose evolutionary history is still poorlyknown. The traditionally defined Lyngbya has been demonstrated to be polyphyletic, including at least five distinctclades, some of which have been proposed as new genera. Intraspecific diversity is also clearly underestimated in Lyngbyadue to the lack of unique morphological characters to differentiate species. In this study, we describe the new genusCapillus T. A. Caires, C. L. Sant’Anna et J. M. C. Nunes from benthic marine environments, including two new Brazilianspecies (here described as C. salinus T. A. Caires, C. L. Sant’Anna et J. M. C. Nunes, and C. tropicalis T. A. Caires, C. L. Sant’Anna et J. M. C. Nunes), and two species yet to be described, one of them from India (Capillus sp. 2.1), and the otherfrom United States of America, based on strain PCC 7419. Capillus species presented cross-wise diagonal fragmentation,assisted or not by necridic cells, which has not been previously mentioned for Lyngbya. Ultrastructural analyses showedthat C. salinus and C. tropicalis have numerous gas vesicles, which are rarely described for benthic marine species. Thenew genus formed a well-supported clade, and the D1-D1′ and Box B secondary structures of internal transcribed spaceralso supported the proposal of its new species. These findings help to clarify the diversity of species in the Lyngbya complexand the taxonomy of the group, and highlight the need of further floristic surveys in tropical coastal environments,which remain poorly studied.

Novel anti-apoptotic mechanism of A20 through targeting ASK1 to suppress TNF-induced JNK activation

Won, M,Park, K A,Byun, H S,Sohn, K-C,Kim, Y-R,Jeon, J,Hong, J H,Park, J,Seok, J H,Kim, J M,Yoon, W-H,Jang, I-S,Shen, H M,Liu, Z G,Hur, G M Macmillan Publishers Limited 2010 CELL DEATH AND DIFFERENTIATION Vol.17 No.12

The zinc-finger protein A20 has crucial physiological functions as a dual inhibitor of nuclear factor-κB (NF-κB) activation and apoptosis in tumor necrosis factor (TNF) receptor 1 signaling pathway. Although the molecular basis for the anti-NF-κB function of A20 has been well elucidated, the anti-apoptotic function of A20 is largely unknown. Here, we report a novel mechanism underlying the anti-apoptotic function of A20: A20 blocks TNF-induced apoptosis through suppression of c-jun N-terminal kinase (JNK) by targeting apoptosis signal-regulating kinase1 (ASK1). First, the ectopic expression of A20 drastically inhibits TNF-induced JNK activation and apoptosis in multiple cell types including those deficient of NF-κB activation. Unexpectedly, the blunting effect of A20 on TNF-induced JNK activation is not mediated by affecting the TNFR1 signaling complex formation. Instead, A20 interacts with ASK1, an important MAPKK kinase in the JNK signaling cascade. More importantly, overexpression of wild-type A20, but not of mutant A20 (ZnF4; C624A, C627A), promotes degradation of the ASK1 through the ubiquitin-proteasome system. Taken together, the results from this study reveal a novel anti-apoptotic mechanism of A20 in TNF signaling pathway: A20 binds to ASK1 and mediates ASK1 degradation, leading to suppression of JNK activation and eventually blockage of apoptosis.

Polyphasic delimitation of a filamentous marine genus, Capillus gen. nov. (Cyanobacteria, Oscillatoriaceae) with the description of two Brazilian species

Caires, Taiara A.,Lyra, Goia de M.,Hentschke, Guilherme S.,da Silva, Aaron Matheus S.,de Araujo, Valter L.,Sant'Anna, Celia L.,Nunes, Jose Marcos de C. The Korean Society of Phycology 2018 ALGAE Vol.33 No.4

Lyngbya C. Agardh ex Gomont is a nonheterocytous cyanobacterial genus whose evolutionary history is still poorly known. The traditionally defined Lyngbya has been demonstrated to be polyphyletic, including at least five distinct clades, some of which have been proposed as new genera. Intraspecific diversity is also clearly underestimated in Lyngbya due to the lack of unique morphological characters to differentiate species. In this study, we describe the new genus Capillus T. A. Caires, C. L. Sant'Anna et J. M. C. Nunes from benthic marine environments, including two new Brazilian species (here described as C. salinus T. A. Caires, C. L. Sant'Anna et J. M. C. Nunes, and C. tropicalis T. A. Caires, C. L. Sant'Anna et J. M. C. Nunes), and two species yet to be described, one of them from India (Capillus sp. 2.1), and the other from United States of America, based on strain PCC 7419. Capillus species presented cross-wise diagonal fragmentation, assisted or not by necridic cells, which has not been previously mentioned for Lyngbya. Ultrastructural analyses showed that C. salinus and C. tropicalis have numerous gas vesicles, which are rarely described for benthic marine species. The new genus formed a well-supported clade, and the D1-D1' and Box B secondary structures of internal transcribed spacer also supported the proposal of its new species. These findings help to clarify the diversity of species in the Lyngbya complex and the taxonomy of the group, and highlight the need of further floristic surveys in tropical coastal environments, which remain poorly studied.

MOA-2010-BLG-073L: AN M-DWARF WITH A SUBSTELLAR COMPANION AT THE PLANET/BROWN DWARF BOUNDARY

Street, R. A.,Choi, J.-Y.,Tsapras, Y.,Han, C.,Furusawa, K.,Hundertmark, M.,Gould, A.,Sumi, T.,Bond, I. A.,Wouters, D.,Zellem, R.,Udalski, A.,Snodgrass, C.,Horne, K.,Dominik, M.,Browne, P.,Kains, N.,Br IOP Publishing 2013 The Astrophysical journal Vol.763 No.1

<P>We present an analysis of the anomalous microlensing event, MOA-2010-BLG-073, announced by the Microlensing Observations in Astrophysics survey on 2010 March 18. This event was remarkable because the source was previously known to be photometrically variable. Analyzing the pre-event source light curve, we demonstrate that it is an irregular variable over timescales >200 days. Its dereddened color, (V - I)(S),(0), is 1.221 +/- 0.051 mag, and from our lens model we derive a source radius of 14.7 +/- 1.3 R-circle dot, suggesting that it is a red giant star. We initially explored a number of purely microlensing models for the event but found a residual gradient in the data taken prior to and after the event. This is likely to be due to the variability of the source rather than part of the lensing event, so we incorporated a slope parameter in our model in order to derive the true parameters of the lensing system. We find that the lensing system has a mass ratio of q = 0.0654 +/- 0.0006. The Einstein crossing time of the event, t(E) = 44.3 +/- 0.1 days, was sufficiently long that the light curve exhibited parallax effects. In addition, the source trajectory relative to the large caustic structure allowed the orbital motion of the lens system to be detected. Combining the parallax with the Einstein radius, we were able to derive the distance to the lens, D-L = 2.8 +/- 0.4 kpc, and the masses of the lensing objects. The primary of the lens is an M-dwarf with M-L,M-1 = 0.16 +/- 0.03 M-circle dot, while the companion has M-L,M-2 = 11.0 +/- 2.0 M-J, putting it in the boundary zone between planets and brown dwarfs.</P>