Effect of sucrose and pectin addition on physical, chemical, thermal and rheologicalproperties of frozen/thawed pineapple pulps

Márcia Cavalcante Conceição,Tatiana Nunes Fernandes,Mônica Elisabeth Torres Prado,Jaime Vilela de Resende 한국유변학회 2012 Korea-Australia rheology journal Vol.24 No.3

Pectin (0-1.0g/100mL) and sucrose (0-20g/100mL) were added to pineapple pulp to improve their rheo\-logical properties, thermal properties and stability after freezing and thawing processes. The properties of the mixes were characterized before and after freezing and thawing. Samples were frozen at -20oC, and the freeze concentration was evaluated every 60min. The thawing rate was evaluated at 19oC and quantified by photographic editing and image analysis software. The thawing rates and values for the freeze con\-centration were leveled out at pectin concentrations above 0.5g/100mL pectin, which indicated that pectin functions to maintain structural homogeneity during freezing. In the thawed samples, the plastic viscosity values were leveled out from pectin concentrations (0.25-0.75g/100mL) as the sucrose concentration increased when compared to unfrozen samples. The differences between the rheological parameters of the unfrozen and frozen/thawed pulps, the higher yield stress values after thawing were attributed to the size of suspended particles in the pulp. Applications can specify formulations of frozen products containing pec\-tin, where these properties can be handled after thawing the product.

Evaluation of the Efficacy of Flaxseed Meal and Flaxseed Extract in Reducing Menopausal Symptoms

Márcia Constantino Colli,Adelar Bracht,Andréa Assunção Soares,Andréa Luisa de Oliveira,Cinthia Gandolfi Bôer,Cristina Giatti Marques de Souza,Rosane Marina Peralta 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.9

The objective of this study was to evaluate the efficacy of flaxseed meal and flaxseed extract in reducing climacteric symptoms of menopausal women. Ninety menopausal women were randomly distributed into three study groups: group I received 1 g per day of flaxseed extract containing at least 100 mg of secoisolariciresinol diglucoside (SDG), group II received 90 g per day of flaxseed meal containing at least 270 mg of SDG, and group III received 1 g per day of collagen (placebo group). Subjects were assessed for menopausal symptoms by the Kupperman index at the beginning and at the end of the 6 months of treatment. Subjects were also assessed for endometrial thickness and vaginal cytology. The Kupperman index values at the beginning and end of the treatments were analyzed using the paired t-test. Both the flaxseed extract (P=.007) and the flaxseed meal (P=.005) were effective in reducing the menopausal symptoms when compared with the placebo control (P=.082). Alternatively, the changes in Kupperman index were also computed and submitted to analysis of variance. In this case, no significant differences were found (P=.084) although the data indicate a decreasing tendency for the Kupperman index by both the flaxseed extract and the flaxseed meal groups. Neither the flaxseed extract nor the flaxseed meal exerted clinically important estrogenic effects on the vaginal epithelium or endometrium as revealed by the absence of changes in the blood levels of follicle stimulating hormone and estradiol, as well as in the endometrial thickness, and vaginal epithelial maturation value. No serious adverse events related to the treatments were reported. Although the results of the present study do not allow an unequivocal conclusion about the action of flaxseed on the menopausal symptoms, they suggest that it could be premature to conclude that no such action exists. Clearly the matter still deserves further experimental attention.

Education of traditional medicine for people with visual impairments in Brazil: Challenges and strategies

Mércia Ferreira Souza,Arthur Sá Ferreira 한국한의학연구원 2021 Integrative Medicine Research Vol.10 No.2

Evaluation of mycelial growth of Lentinula edodes and Ganoderma lucidum originated from South Korea and Brazil

Márcia Araújo Motta,Jeonghwa Jang,Mijeong Kim,Eunsil Choi,Jiyoung Kim,Anna Carolina Torracca-Rocha,Won-Sik Kong,Arailde Fontes Urban 한국버섯학회 2017 한국버섯학회지 Vol.15 No.2

The choice of strain, substrate, and environmental conditions are fundamental factors that determine the ability of a fungus to develop and produce mushrooms with high quality. The objective of this study was to compare the strains of Lentinula edodes and Ganoderma lucidum originated from South Korea and Brazil in relation to their vegetative development in different culture media, to obtain isolates with high quality. The strains of the two mushroom species from Brazil and South Korea grew slowly, possibly because of abiotic factors or storage technique used. However, it may be concluded that temperature, humidity, light, pH, and nutrients from the substrate, if not regulated appropriately may affect the vegetative species.

Color discrepancy of single-shade composites at different distances from the interface measured using cell phone images

Santana Márcia Luciana Carregosa,Livi Gabriella de Jesus Santos,Faria-e-Silva André Luis 대한치과보존학회 2024 Restorative Dentistry & Endodontics Vol.49 No.1

Objectives This study aimed to evaluate the impact of substrate color and interface distance on the color adjustment of 2 single-shade composites, Vittra APS Unique and Charisma Diamond One. Materials and Methods Dual disc-shaped specimens were created using Vittra APS Unique or Charisma Diamond One as the center composite, surrounded by shaded composites (A1 or A3). Color measurements were taken with a spectrophotometer against a gray background, recording the color coordinates in the CIELAB color space. Illumination with a light-correcting device and image acquisition using a polarizing filter-equipped cell phone were performed on specimens over the same background. Image processing software was used to measure the color coordinates in the center and periphery of the inner composite and in the outer composite. The color data were then converted to CIELAB coordinates and adjusted using data from the spectrophotometer. Color differences (ΔE00) between the center/periphery of single-shade and outer composites were calculated, along with color changes in single-shade composites caused by different outer composites. Color differences for the inner composites surrounded by A1 and A3 were also calculated. Data were analyzed using repeated-measures analysis of variance (α = 0.05). Results The results showed that color discrepancies were lowest near the interface and when the outer composite was whiter (A1). Additionally, Charisma Diamond One exhibited better color adjustment ability than Vittra APS Unique. Conclusions Color discrepancies between the investigated single-shade composites diminished towards the interface with the surrounding composite, particularly when the latter exhibited a lighter shade. Objectives This study aimed to evaluate the impact of substrate color and interface distance on the color adjustment of 2 single-shade composites, Vittra APS Unique and Charisma Diamond One. Materials and Methods Dual disc-shaped specimens were created using Vittra APS Unique or Charisma Diamond One as the center composite, surrounded by shaded composites (A1 or A3). Color measurements were taken with a spectrophotometer against a gray background, recording the color coordinates in the CIELAB color space. Illumination with a light-correcting device and image acquisition using a polarizing filter-equipped cell phone were performed on specimens over the same background. Image processing software was used to measure the color coordinates in the center and periphery of the inner composite and in the outer composite. The color data were then converted to CIELAB coordinates and adjusted using data from the spectrophotometer. Color differences (ΔE00) between the center/periphery of single-shade and outer composites were calculated, along with color changes in single-shade composites caused by different outer composites. Color differences for the inner composites surrounded by A1 and A3 were also calculated. Data were analyzed using repeated-measures analysis of variance (α = 0.05). Results The results showed that color discrepancies were lowest near the interface and when the outer composite was whiter (A1). Additionally, Charisma Diamond One exhibited better color adjustment ability than Vittra APS Unique. Conclusions Color discrepancies between the investigated single-shade composites diminished towards the interface with the surrounding composite, particularly when the latter exhibited a lighter shade.

Environmental Management: Solid Waste Policy in Brazil

( Marchi Cristina M D F ),( Trocoli Márcia J. M. ) 한국폐기물자원순환학회 2011 ISWA Vol.2011 No.0

Assessment of Effects of a Cordia salicifolia Extract on the Radiolabeling of Blood Constituents and on the Morphology of Red Blood Cells

Jacques Natan Grinapel Frydman,Vanessa Camara Rocha,Monica Oliveira Benarroz,Gabrielle Souza Rocha,Márcia Oliveira Pereira,Adenilson de Souza da Fonseca,Mario Bernardo-Filho 한국식품영양과학회 2008 Journal of medicinal food Vol.11 No.4

Effects of a Cordia salicifolia (porangaba) extract on the labeling of blood cells (BCs) with technetium-99m (99mTc) and on the morphology of red BCs were evaluated. Labeling of cellular and molecular structures with 99mTc depends on a reducing agent. Some physical characteristics, as visible absorbance spectrum, electric conductivity, and refractive index of this porangaba extract, were also determined. Blood samples from Wistar rats were incubated with porangaba extract or with 0.9% NaCl (control). Labeling of blood constituents with 99mTc was performed. Plasma (P) and BCs, both soluble (SF-P and SF-BC) and insoluble (IF-P and IF-BC) fractions, were separated. The radioactivity in each fraction was counted, and the percentage of radioactivity incorporated (%ATI) was calculated. Blood smears were prepared, fixed, and stained, and the morphology of the red BCs was evaluated. Data showed an absorbance peak at 480 nm and electric conductibility and refractive index concentration-dependent. Porangaba extract decreased significantly (P < .05) the BC, IF-P, and IF-BC %ATI, and no modifications were verified on the shape of red BCs. Analysis of the results reveals that some physical parameters could be useful to aid in characterizing the extract studied. Moreover, it is possible that chemical compounds of this extract could have chelating/redox actions or be capable of binding to plasma and/or cellular proteins.

Depression and Mania Induce Pro-inflammatory Activation of Macrophages Following Application of Serum from Individuals with Bipolar Disorder

Pamela Ferrari,Mariana Migliorini Parisi,Rafael Colombo,Matheus Becker,Gabriel Fries,Bruna Maria Ascoli,Luiza Paul Géa,Márcia Kauer-Sant’anna,Flávio Kapczinski,Fábio Klamt,Fátima T.C.R. Guma,Adriane R 대한정신약물학회 2018 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.16 No.1

Objective: Evidence has suggested that immune imbalance is involved with bipolar disorder (BD); however, its precise mechanism is poorly understood. This study investigated whether biochemical changes in the serum from BD patients could modulate the phenotype of cultured macrophages. Methods: Eighteen subjects with BD and five healthy individuals were included in this study. The human monocyte cell line U-937 was activated with phorbol 12-myristate 13-acetate (PMA) and polarization was induced with RPMI-1640 media supplemented with 10% serum from each patient for 24 hours. Gene expression of selected M1 and M2 markers was assessed by quantitative PCR. Results: Macrophages exposed to serum of manic and depressive BD patients displayed an increase of interleukin-1 (6.40±3.47 and 9.04±5.84 vs. 0.23±0.11; p<0.05) and tumor necrosis factor- (2.23±0.91 and 2.03±0.45 vs. 0.62±0.24; p=0.002 and p=0.004, respectively) compared to euthymic group (there was no difference between euthymic and controls). In parallel, U-937 macrophages treated with serum of patients in acute episode displayed a down-regulation of CXCL9 (0.29±0.20 vs. 1.86±1.61; p=0.006) and CXCL10 expression (0.36±0.15 and 0.86±0.24 vs. 1.83±0.88; p<0.000 and p=0.04) compared to the euthymia group. Conclusion: Our results are consistent with previous studies showing that changes in peripheral blood markers could modulate M1/M2 polarization in BD. The evidence of macrophages as source of inflammatory cytokines might be helpful to unravel how the mononuclear phagocyte system is involved in the etiology of BD.

Jaboticaba (Myrciaria cauliflora) Peel Supplementation Prevents Hepatic Steatosis Through Hypolipidemic Effects and Cholesterol Metabolism Modulation in Diet-Induced Nonalcoholic Fatty Liver Disease Rat Model

Nara Nunes Lage,Mayara Medeiros de Freitas Carvalho,Joyce Ferreira da Costa Guerra,Juliana Márcia Macedo Lopes,Renata Rebeca Pereira,Ana Carolina Silveira Rabelo,Vinícius Marques Arruda,Mariana de Fát 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.9

Jaboticaba (Myrciaria cauliflora), a Brazilian fruit, is a good source of dietary fiber and phenolic compounds, which are concentrated mainly in the peel. These compounds have been considered promising in prevention and treatment of hypercholesterolemia and hepatic steatosis. In this study, we investigated the effects of 4% jaboticaba peel powder (JPP) supplementation on cholesterol metabolism and hepatic steatosis in livers of rats fed a high-fat (HF) diet. The rats were fed a standard AIN-93M (control) diet or an HF diet containing 32% lard and 1% cholesterol, both with and without 4% JPP. The M. cauliflora peel composition revealed a low-lipid high-fiber content and phenolic compounds. The phenolic compounds in JPP, tentatively identified by high-performance liquid chromatography and mass spectrometry (HPLC-MS/MS) analysis, were confirmed to contain phenolic acids, flavonoids, and anthocyanins. Moreover, JPP presented significant antioxidant activity in vitro and was not cytotoxic to HepG2 cells, as determined by the lactate dehydrogenase (LDH) assay. After 6 weeks of treatment, our results showed that JPP supplementation increased lipid excretion in feces, reduced serum levels of total cholesterol and nonhigh-density lipoprotein cholesterol, decreased serum aspartate aminotransferase (AST) activity, and attenuated hepatic steatosis severity in rats fed the HF diet. Furthermore, JPP treatment downregulated expression of ACAT-1, LXR-α, CYP7A1, and ABCG5 genes. Therefore, jaboticaba peel may represent a viable dietary strategy to prevent nonalcoholic fatty liver disease as the JPP treatment alleviated hepatic steatosis through improvement of serum lipid profiles and modulation of mRNA expression of genes involved in cholesterol metabolism.

Cytogenotoxic study of aqueous fruit extract of Morinda citrifolia in Wistar albino rats

Germano Pinho de Moraes,Marcus Vinícius Oliveira Barros de Alencar,Lidiane da Silva Araújo,Débora Cássia Vieira Gomes,Márcia Fernanda Correia Jardim Paz,Muhammad Torequl Islam,Ana Amélia de Carvalho M 경희대학교 융합한의과학연구소 2019 Oriental Pharmacy and Experimental Medicine Vol.19 No.3

The use of herbal products and/or their derivatives as an alternative or complementary medicine becomes globally popular. To date, various biological activities have been reported in Morinda citrifolia Linn, especially antioxidants, antimicrobial, anti-infammatory and antitumor efects. This study aimed to investigate the genotoxicity and DNA repair capacity of the aqueous fruit extract of M. citrifolia (AEMC). Additionally, we checked the mutagenic capacity of AEMC by the comet assay and micronucleus tests in rats. For this, rats (n=10; 5 males and 5 females) were divided into fve groups as: negative control (distilled water); positive control (25 mg/kg of cyclophosphamide, i.p.); and three groups for AEMC (2.5, 5 and 10 mg/kg, i.p.). The results suggest that, the AEMC induced genotoxicity in white blood cells, but a DNA repair capacity was also observed after 72 h of AEMC exposure. AEMC also showed cytotoxicity and mutagenicity in liver and kidney cells of rats at 5 and 10 mg/kg doses. In conclusion, AEMC exhibited a dose-dependent cytotoxicity, genotoxicity and mutagenicity along with a time-dependent DNA repair capacity in rats. Further studies are necessary regarding the safe use of this medicinal plant for human consumption.