Chemical Composition and Antinociceptive Potential of Campomanesia reitziana Fruits

Luciane Angela Nottar Nesello,Adriana Campos,Theodoro Wagner,Arturo San Feliciano,Fatima de Campos Buzzi,Valdir Cechinel Filho 한국식품영양과학회 2016 Journal of medicinal food Vol.19 No.5

The methanolic extract from Campomanesia reitziana fruits and the main active principle, identified as 4',6'-dihydroxy-3',5'-dimethyl-2'-methoxy chalcone or dimethyl cardamonin (1), exhibited pronounced antinociceptive effects against two models of pain in mice. Compound 1 caused dose-dependent inhibition of abdominal constrictions, with a calculated ID50 value of 8.1 (6.5–10.1) μmol/kg (i.p.), being about 16-fold more potent than two reference analgesic drugs. Methanolic extract and 1 were also effective against the formalin model, inhibiting both phases of pain, causing reductions of 39.9% and 26.8% (extract, 10 mg/kg) and 52.9% and 57.6% (compound 1, 5 mg/kg) for the first and second phases, respectively.

GLUT2 proteins and PPARγ transcripts levels are increased in liver of ovariectomized rats: reversal effects of resistance training

Luciane M. Tomaz,Jean-Marc Lavoie,Marina R. Barbosa,Zahra Farahnak,Cristiani G. Lagoeiro,Natalia S.S Magosso,Sérgio E. A. Perez 한국운동영양학회 2016 Physical Activity and Nutrition (Phys Act Nutr) Vol.20 No.2

Purpose: This study investigated the effects of ovariectomy (Ovx) and 12 weeks of resistance training (RT) on gene expression of GLUT2, the main glucose transporter in the liver, and on PPARγ, a transcription factor known to target GLUT2 expression. Methods: Forty Holtzman rats were divided into 5 groups: Sham-sedentary (Sed), Sham-RT, Ovx-Sed, Ovx-RT, and Ovx-Sed with hormone replacement (E2). The RT protocol consisted of sessions held every 72 h for 12 weeks, during which the animals performed 4 to 9 vertical climbs (1.1 m) at 2 min intervals with progressively heavier weights (30 g after the fourth climb) tied to the tail. The E2 silastic capsule was inserted into the rats’ backs 48 hours before the first RT session. Results: In addition to liver fat, GLUT2 protein levels and PPARγ transcripts were increased (P < 0.05) in Ovx compared to Sham-Sed animals, suggesting increased hepatic glucose uptake under estrogen deficient conditions. RT and E2 in Ovx rats decreased liver fat accumulation as well as GLUT2 and PPARγ gene expression to the level of Sham-Sed animals. Conclusion: The results of this study suggest that liver GLUT2 as well as PPARγ expression in Ovx rats are accompanied by increased fat accumulation and glucose uptake, thus providing a substrate for increased de novo lipogenesis. RT appears to be an appropriate exercise model to circumvent these effects.

How to perform a functional assessment of the fetal heart: a pictorial review

Luciane Alves Rocha,Liliam Cristine Rolo,Edward Araujo Júnior 대한초음파의학회 2019 ULTRASONOGRAPHY Vol.38 No.4

The purpose of this pictorial review was to describe various echocardiographic techniques that can be used for the functional assessment of the fetal heart. The systolic and diastolic assessments of the fetal heart are presented separately, with an emphasis on 2-dimensional Doppler methods and an overview of new technologies. The aim of this summary was to review the tools that can be used by the echocardiographer, and on that basis, to systematize the process of performing a functional assessment.

Welfare traits of Bos indicus cattle castrated immunologically and fed beta-adrenergic agonists

Martello, Luciane Silva,Antonelo, Daniel Silva,Consolo, Nara Regina Brandao,Pacheco, Veronica Madeira,Negrao, Joao Alberto,Rosa, Alessandra Fernandes,Leme, Paulo Roberto,Sousa, Rafael Vieira,Silva, Sa Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.9

Objective: This work was carried out to evaluate the effects of zilpaterol hydrochloride (ZH) and ractopamine hydrochloride (RH) combined with immunocastration on the welfare traits of feedlot Nellore cattle. Methods: Ninety-six Nellore males (average body weight [BW] = 409±50 kg; average 20 mo of age) were divided into two groups according to BW; half of the animals in each group received two doses of an immunocastration (ImC) vaccine in a 30 day interval, and the other half did not receive the vaccine (NoC). Afterward, the animals were housed and fed a common diet for 70 days. Then, they were split into three groups and fed one of the following diets for 30 additional days: control (CO) diet, with no β-AA; ZH diet, containing 80 mg/d ZH; and RH diet, containing 300 mg/d RH. Welfare traits were assessed by monitoring body surface temperature using infrared thermography (IRT) and plasma cortisol and temperament measurements. Results: There was no interaction between sexual condition and diet for any trait. The ImC and NoC groups did not differ in rectal and ocular temperatures. The ImC animals had higher flight speeds (p = 0.022) and tended to have higher cortisol levels (p = 0.059) than the NoC animals. Animals fed ZH and RH did not differ in cortisol levels, respiratory rate, rectal temperature, temperature measured by IRT, or temperament behaviour. Conclusion: The ImC animals showed a less stable temperament during handling practices than NoC, whereas ZH and RH supplementation had no adverse effects on animal welfare.

교정치료 초기에 사용되는 4가지 호선의 초기 치료효과를 비교하기 위한 전향적 임상 실험 연구

unknown,Malcolm L. Jones,Luciane M. Menezes,Koo, Daniel,Carlos N. Elias 대한치과교정학회 2005 대한치과교정학회지 Vol.35 No.5

본 연구의 목적은 교정치료 초기에 사용되는 스테인레스스틸, 다가닥철선, 초탄성 NiTi, 열활동성 NiTi 재료로 이루어진 총 4가지 호선의 초기 치료효과를 비교하기 위하여 시행되어으며, 실험의 설계는 전향적 임상 실험(prospective randomized clinical trial)으로서 브라질 리오데자네이로 주립 치과대학에 내원한 45명의 고정식 교정장치 환자를 대상으로 시행되었다. 각 호선의 재료는 환자의 치열에 무작위로 배당되었는데 스테인레스스틸은 26명,다가닥철선은 22명,초탄성NiTi는 22명, 열활동성NiTi는 20명에게 할당되었고.8주후에 모형을 다시 제작한 후 3차원 디지털 영상 장비를 이용하여 모형의 치관에 설정된 해부학적 지표의 변화를 측정하였는데 치료전 및 치료후 치열불규칙지수(Dental Irregularlty Index)의 차이로 초기 교정치료 효과를 비교하였다. 분산분석을 시행하여 불규칙지수의 변화를 살펴본 결과 호선의 재료에 따른 초기 치료 효과는 유의한 차이가 나타나지 않았다. The aim of this study was to compare the clinical performance of 4 types of orthodontic wires, indicated for initial tooth alignment: stainless steel, multistranded steel, superelastic and thermoactivated nickel-titanium. A prospective randomized clinical trial was conducted on a sample of 45 patients, at the Dental School of the State University of Rio de Janeiro, Brazil. Fixed appliances were fitted and study casts were obtained from each patient. Randomly, the wires were allocated as follows: 26dental arches for superelastic NiTi wires, 22 for stainless steel, 22 for multistranded and 20 for thermoactivated archwires. After 8 weeks, the archwires were removed and impressions for study casts were taken again. Using a 3D digitization technique of defined anatomical points on the study cast crowns, a Dental irregularity Index (Dll) was created for each study cast. The difference between Dll before and after the archwire insertion expressed the aligning effect of the wires. ANOVA tests were employed to evaluate the anatomical point approximation (positive Dll) and separation (negative Dll), for each area of the dental arches: upper and lower whole arch and anterior arch. Results showed no significant difference between the different archwires.

Production of Bioactive Compounds with Antitumor Activity Against Sarcoma 180 by Pleurotus sajor-caju

Ivaneliza Simionato Assis,Mariane Bonatti Chaves,Marcia Luciane Lange Silveira,Regina Maria Miranda Gern,Elisabeth Wisbeck,Agenor Furigo Ju´nior,Sandra Aparecida Furlan 한국식품영양과학회 2013 Journal of medicinal food Vol.16 No.11

This work studied the influence of culture medium composition and pH on exopolysaccharide (EPS) production by Pleurotus sajor-caju and validates the antitumor activity of the produced EPSs and of the mycelial biomass (intracellular polysaccharides [IPSs]) against Sarcoma 180 (S180) cells. The effect of the initial concentrations of (NH4)2SO4, yeast extract and soy peptone on EPS production by P. sajor-caju was studied in shake flasks. A bioreactor was used to evaluate the pH values and the initial CaCO3 and glucose concentrations. Extracts of EPSs (PE1) and IPSs obtained through two different separation processes (PM1 and PM2) were tested on mice inoculated with S180 cells. A medium containing 2.5, 1.0, and 1.0 g/L of (NH4)2SO4, yeast extract and soy peptone, respectively, provided the highest EPS concentration (0.6 g/L). The use of pH 4.0, 1.0 g/L CaCO3 and 20 g/L initial glucose concentration enhanced EPS productivity (3.84 g/L per hour). The PE1 extract promoted the highest reduction of S180 growth (86%), followed by the PM2 extract (80%); growth reduction was doseindependent for both substances. This work provides information about culture medium and conditions that enhanced the production of extracellular polysaccharides by P. sajor-caju. The results can contribute to the search for new bioactive products bringing novel aspects to the medical and pharmaceutical areas.

Green carbon dots synthesized from Chlorella Sorokiniana microalgae biochar for chrome detection

Aline C.C. Pena,Lucas Manique Raymundo,Luciane F. Trierweiler,Mariliz Gutterres 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.117 No.-

In the quest to find an economical and accurate method of detecting toxic metals as well as finding anapplication for biochar as a fuel, this work used microalgae biochar to sinter Carbon Dots (CDs) thatcan be used as pathways for metallic ions detection. Carbon Dots from microalgae biochar (generatedin pyrolysis) were synthesized through thermochemical depolymerization with KMnO4 at different concentrations. After this step, the purification of the nanoparticles was performed. The characterization ofCDs was performed through fluorescence intensity, Zeta Potential, Fourier Transform InfraredSpectroscopy (FTIR), and atomic force microscopy (AFM). The results showed that all samples exhibitedfluorescence, samples synthesized with KMnO4 at concentrations of 8.54 % and 10.0 % (CD-MBK06 andCD-MBK07, respectively) showed the highest fluorescence intensity and good stability. When addingCr (VI) and Cr (III) to the CD-MBK06 and CD-MBK07 samples, it is possible to notice that there was fluorescencequenching as the metal ion concentration increased, except for the CD-MBK07 sample for Cr(III), at a length of 350 nm, indicating that this sample shows selectivity for extinction only for Cr (VI).

FE analysis of RC pipes under three-edge-bearing test: Pocket and diameter influence

Marcela Novischi Kataoka,Jefferson Lins da Silva,Luciane Marcela Filizola de Oliveira,Mounir Khalil El Debs 사단법인 한국계산역학회 2017 Computers and Concrete, An International Journal Vol.20 No.4

This paper studies on the behavior of reinforced concrete (RC) pipes used in basic sanitation in the conduction of storm water and sanitary sewer. Pipes with 800 mm and 1200 mm in diameter were analyzed. The 800 mm pipes were built with simple reinforcement and the 1200 mm pipes with double reinforcement. For the two diameters of pipes the presence or absence of the pocket was evaluated, and the denomination of each one is spigot and pocket pipe (SPP) and ogee joint pipe (OJP), respectively. The 3D numerical models reproduce the three-edge-bearing test that provides information about the strength and stiffness of the reinforced concrete pipes. The validation of the computational models was carried out comparing the vertical and horizontal displacements on the springline and crown/invert and it was also evaluated the reinforcement strains and the crack pattern. As a main conclusion, the numerical models represented satisfactorily the behavior of the pipes and can be used in future studies in parametric analysis.

Exploratory Study of Epidermis, Basement Membrane Zone, Upper Dermis Alterations and Wnt Pathway Activation in Melasma Compared to Adjacent and Retroauricular Skin

( Ana Cláudia Cavalcante Espósito ),( Gabrielli Brianezi ),( Nathália Pereira De Souza ),( Luciane Donida Bartoli Miot ),( Hélio Amante Miot ) 대한피부과학회 2020 Annals of Dermatology Vol.32 No.2

Background: Melasma is a chronic acquired focal hypermelanosis which pathogenesis has not been fully elucidated. Classical pathophysiologic studies have analysed the affected and perilesional areas, but little is known about the status of sun-protected skin, which is subjected to the same endogenous and genetic factors. Objective: To assess the histological characteristics of melasma compared to adjacent and retroauricular skin. Methods: Skin samples were collected from 10 female from: melasma, perilesional area and retroauricular. The samples were stained (haematoxylin-eosin, periodic acid-Schiff, Fontana-Masson, picrosirius red, toluidine blue and Verhoeff), immunolabelled for CD34 and Wnt1. The data from the skin sites were analysed simultaneously by a multivariate model. Results: Melasma skin exhibited noteworthy stratum corneum compaction, greater collagen heterogeneity, solar elastosis, higher number of mast cells, basement membrane zone (BMZ) damage, Wnt1 expression, pendulum melanocytes, higher cellularity and vascular proliferation at the superficial dermis. Stratum corneum compaction, collagen heterogeneity and BMZ abnormalities were variables associated to melasma that not follow a continuum through retroauricular to adjacent skin. Mast cell count was the variable that disclosed correlation with the most other abnormalities as well as had the greater contribution in the multivariate model. Conclusion: In addition to melanocyte hyperactivity, melasma skin exhibits alterations in the epidermal barrier, upper dermis and BMZ, which differ from the adjacent sun-exposed skin and retroauricular skin, indicating a distinct phenotype, rather than a mere extension of photoageing or intrinsic ageing. Mast cells appear to play a central role in the physiopathology of melasma. (Ann Dermatol 32(2) 101∼108, 2020)

Effect of number of uses and sterilization on the instrumented area and resistance of reciprocating instruments

Peraça Victor de Ornelas,Xavier Samantha Rodrigues,de Almeida Gomes Fabio,dos Santos Luciane Geanini Pena,Souza Erick Miranda,Pappen Fernanda Geraldo 대한치과보존학회 2021 Restorative Dentistry & Endodontics Vol.46 No.2

Objectives: This study evaluated the effect of repeated uses and autoclaving in the instrumented area, fracture resistance, and time of instrumentation of thermally treated nickel-titanium reciprocating systems. Materials and Methods: Two hundred simulated canals were instrumented using Reciproc Blue and WaveOne Gold. Each file was used up to 10 times or until fracture. The instrumented area was measured in pre- and post-operative images, using ImageJ software. Kaplan-Meier survival analysis evaluated the number of uses of instruments before fracture. Instrumented area and time of instrumentation were analyzed by Mann-Whitney U test and Kruskal-Wallis. Correlations among the number of uses and instrumented area were measured. The level of statistical significance was set at p < 0.05. Results: Reciproc Blue presented a higher estimated number of uses in comparison with WaveOne Gold (p = 0.026), but autoclaving did not affect the resistance to fracture of instruments (p > 0.05). The instrumented area was different among the evaluated groups (p = 0.039), and the instrumented area along the uses of both tested instruments was reduced. With the time of instrumentation, there was also a significant difference among the evaluated groups; the groups without sterilization cycles were faster, in comparison to those submitted to autoclaving (p = 0.010). Conclusions: Reciproc Blue was more resistant than WaveOne Gold, suffering later fracture. Additionally, the sterilization cycles did not influence the estimated number of uses of thermally treated reciprocating instruments, but the instrumented area of root canals was reduced along with the repeated uses of both instruments.