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Adriana Sturion Lorenzi,Mathias Ahii Chia,Fabyano Alvares Cardoso Lopes,Genivaldo Gueiros Z. Silva,Robert A. Edwards,Maria do Carmo Bittencourt-Oliveira 한국미생물학회 2019 The journal of microbiology Vol.57 No.6
Next-generation DNA sequencing technology was applied to generate molecular data from semiarid reservoirs during well-defined seasons. Target sequences of 16S-23S rRNA ITS and cpcBA-IGS were used to reveal the taxonomic groups of cyanobacteria present in the samples, and genes coding for cyanotoxins such as microcystins (mcyE), saxitoxins (sxtA), and cylindrospermopsins (cyrJ) were investigated. The presence of saxitoxins in the environmental samples was evaluated using ELISA kit. Taxonomic analyses of high-throughput DNA sequencing data showed the dominance of the genus Microcystis in Mundaú reservoir. Furthermore, it was the most abundant genus in the dry season in Ingazeira reservoir. In the rainy season, 16S-23S rRNA ITS analysis revealed that Cylindrospermopsis raciborskii comprised 46.8% of the cyanobacterial community in Ingazeira reservoir, while the cpcBAIGS region revealed that C. raciborskii (31.8%) was the most abundant taxon followed by Sphaerospermopsis aphanizomenoides (17.3%) and Planktothrix zahidii (16.6%). Despite the presence of other potential toxin-producing genera, the detected sxtA gene belonged to C. raciborskii, while the mcyE gene belonged to Microcystis in both reservoirs. The detected mcyE gene had good correlation with MC content, while the amplification of the sxtA gene was related to the presence of STX. The cyrJ gene was not detected in these samples. Using DNA analyses, our results showed that the cyanobacterial composition of Mundaú reservoir was similar in successive dry seasons, and it varied between seasons in Ingazeira reservoir. In addition, our data suggest that some biases of analysis influenced the cyanobacterial communities seen in the NGS output of Ingazeira reservoir.
Study of Chloride Corrosion Organic Inhibitors in Alkaline Pore Solution
( M. Cabrini ),( S. Lorenzi ),( T. Pastore ),( S. Pellegrini ) 한국부식방식학회(구 한국부식학회) 2018 Corrosion Science and Technology Vol.17 No.5
This paper compares the inhibition properties of aspartic and lactic acid salts with nitrite ions and their effect on critical chloride concentration. The tests were carried employing carbon steel specimens in saturated lime solution with varying pH in the range between13 to 13.6. The critical chloride concentration was estimated through multiple specimen potentiostatic tests at potentials in the usual range for passive rebar in the alkaline concrete of atmospheric structures. During tests, chloride salt was added every 48 h until all the specimens showed localized attacks. The cumulative distribution curves, i.e. the number of corroded specimens as a function of the chlorides concentration was obtained. Furthermore, IR spectra were recorded for the evaluation of the presence of the organic inhibitors on the passivity film. The results confirmed the inhibitory effect of 0.1M aspartate comparable with nitrite ions, at a similar concentration. Addition of calcium lactate did not result in an increase in the critical chloride concentration. However, the formation of a massive scale containing the substance that could reduce the corrosion propagation was observed.
Preliminary analysis and design of the heat exchangers for the Molten Salt Fast Reactor
Ronco, Andrea Di,Cammi, Antonio,Lorenzi, Stefano Korean Nuclear Society 2020 Nuclear Engineering and Technology Vol.52 No.1
Despite the recent growth of interest in molten salt reactor technology and the crucial role which heat transfer plays in the design of power reactors, specific studies on the design of heat exchangers for the Molten Salt Fast Reactor have not yet been performed. In this work we deliver a preliminary but quantitative analysis of the intermediate heat exchangers, based on reference design data from the SAMOFAR H2020-Euratom project. Two different promising reference technologies are selected for study thanks to their compactness features, the Printed Circuit and the Helical Coil heat exchangers. We present preliminary design results for each technology, based on simplified design tools. Results highlight the limiting effects of the compactness constraints imposed on the fuel salt inventory and the allowed size. Large pressure drops on both flow sides are to be expected, with negative consequences on pumping power and natural circulation capabilities. The small size required for the flow channels also represents possible fabrication issues and safety concerns regarding channel blockage.
Cechet, A.,Altieri, S.,Barani, T.,Cognini, L.,Lorenzi, S.,Magni, A.,Pizzocri, D.,Luzzi, L. Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.6
In light of the importance of helium production in influencing the behaviour of fast reactor fuels, in this work we present a burn-up module with the objective to calculate the production of helium in both in-pile and out-of-pile conditions tracking the evolution of 23 alpha-decaying actinides. This burn-up module relies on average microscopic cross-section look-up tables generated via SERPENT high-fidelity calculations and involves the solution of the system of Bateman equations for the selected set of actinide nuclides. The results of the burn-up module are verified in terms of evolution of actinide and helium concentrations by comparing them with the high-fidelity ones from SERPENT, considering two representative test cases of (U,Pu)O<sub>2</sub> fuel in fast reactor conditions. In addition, a code-to-code comparison is made with the independent state-of-the-art module TUBRNP (implemented in the TRANSURANUS fuel performance code) for the same test cases. The herein presented burn-up module is available in the SCIANTIX code, designed for coupling with fuel performance codes.
A reduced order model for fission gas diffusion in columnar grains
Pizzocri D.,Di Gennaro M.,Barani T.,Silva F.A.B.,Zullo G.,Lorenzi S.,Cammi A. 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.11
In fast reactors, restructuring of the fuel micro-structure driven by high temperature and high temperature gradient can cause the formation of columnar grains. The non-spheroidal shape and the non-uniform temperature field in such columnar grains implies that standard models for fission gas diffusion can not be applied. To tackle this issue, we present a reduced order model for the fission gas diffusion process which is applicable in different geometries and with non-uniform temperature fields, maintaining a computational requirement in line with its application in fuel performance codes. This innovative application of reduced order models as meso-scale tools within fuel performance codes represents a first-of-a-kind achievement that can be extended beyond fission gas behaviour.
The TANDEM Euratom project: Context, objectives and workplan
Vaglio-Gaudard C.,Dominguez Bautista M.T.,Frignani M.,Fütterer M.,Goicea A.,Hanus E.,Hollands T.,Lombardo C.,Lorenzi S.,Miss J.,Pavel G.,Pucciarelli A.,Ricotti M.,Ruby A.,Schneidesch C.,Sholomitsky S. 한국원자력학회 2024 Nuclear Engineering and Technology Vol.56 No.3
The TANDEM project is a European initiative funded under the EURATOM program. The project started on September 2022 and has a duration of 36 months. TANDEM stands for Small Modular ReacTor for a European sAfe aNd Decarbonized Energy Mix. Small Modular Reactors (SMRs) can be hybridized with other energy sources, storage systems and energy conversion applications to provide electricity, heat and hydrogen. Hybrid energy systems have the potential to strongly contribute to the energy decarbonization targeting carbon-neutrality in Europe by 2050. However, the integration of nuclear reactors, particularly SMRs, in hybrid energy systems, is a new R&D topic to be investigated. In this context, the TANDEM project aims to develop assessments and tools to facilitate the safe and efficient integration of SMRs into low-carbon hybrid energy systems. An open-source “TANDEM” model library of hybrid system components will be developed in Modelica language which, by coupling, will extend the capabilities of existing tools implemented in the project. The project proposes to specifically address the safety issues of SMRs related to their integration into hybrid energy systems, involving specific interactions between SMRs and the rest of the hybrid systems; new initiating events may have to be considered in the safety approach. TANDEM will study two hybrid systems covering the main trends of the European energy policy and market evolution at 2035’s horizon: a district heating network and power supply in a large urban area, and an energy hub serving energy conversion systems, including hydrogen production; the energy hub is inspired from a harbor-like infrastructure. TANDEM will provide assessments on SMR safety, hybrid system operationality and techno-economics. Societal considerations will also be encased by analyzing European citizen engagement in SMR technology safety.
Noncanonical role of singleminded-2s in mitochondrial respiratory chain formation in breast cancer
Wall Steven W.,Sanchez Lilia,Tuttle Kelly Scribner,Pearson Scott J.,Soma Shivatheja,Wyatt Garhett L.,Carter Hannah N.,Jenschke Ramsey M.,Tan Lin,Martinez Sara A.,Lorenzi Philip L.,Gohil Vishal M.,Rijn 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-
Dysregulation of cellular metabolism is a hallmark of breast cancer progression and is associated with metastasis and therapeutic resistance. Here, we show that the breast tumor suppressor gene SIM2 promotes mitochondrial oxidative phosphorylation (OXPHOS) using breast cancer cell line models. Mechanistically, we found that SIM2s functions not as a transcription factor but localizes to mitochondria and directly interacts with the mitochondrial respiratory chain (MRC) to facilitate functional supercomplex (SC) formation. Loss of SIM2s expression disrupts SC formation through destabilization of MRC Complex III, leading to inhibition of electron transport, although Complex I (CI) activity is retained. A metabolomic analysis showed that knockout of SIM2s leads to a compensatory increase in ATP production through glycolysis and accelerated glutamine-driven TCA cycle production of NADH, creating a favorable environment for high cell proliferation. Our findings indicate that SIM2s is a novel stabilizing factor required for SC assembly, providing insight into the impact of the MRC on metabolic adaptation and breast cancer progression.