Soil and Climate Effects on Cowpea Rhizobial Diversity in Pernambuco

Thiago Pontes Lira,Amanda Cordeiro Melo Souza,Tamiris Kempner,Mario Andrade Lira Junior 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

A Single-Tube Multiplexed Assay for Detecting ALK, ROS1, and RET Fusions in Lung Cancer

Lira, M.E.,Choi, Y.L.,Lim, S.M.,Deng, S.,Huang, D.,Ozeck, M.,Han, J.,Jeong, J.Y.,Shim, H.S.,Cho, B.C.,Kim, J.,Ahn, M.J.,Mao, M. American Society for Investigative Pathology and t 2014 The Journal of Molecular Diagnostics Vol.16 No.2

Approximately 7% of non-small cell lung carcinomas (NSCLCs) harbor oncogenic fusions involving ALK, ROS1, and RET. Although tumors harboring ALK fusions are highly sensitive to crizotinib, emerging preclinical and clinical data demonstrate that patients with ROS1 or RET fusions may also benefit from inhibitors targeting these kinases. Using a transcript-based method, we designed a combination of 3' overexpression and fusion-specific detection strategies to detect ALK, ROS1 and RET fusion transcripts in NSCLC tumors. We validated the assay in 295 NSCLC specimens and showed that the assay is highly sensitive and specific. ALK results were 100% concordant with fluorescence in situ hybridization (FISH) (n = 52) and 97.8% concordant with IHC (n = 179) [sensitivity, 96.8% (95% CI 91.0%-98.9%); specificity, 98.8% (95% CI 93.6%-99.8%)]. For ROS1 and RET, we also observed 100% concordance with FISH (n = 46 and n = 15, respectively). We identified seven ROS1 and 14 RET fusion-positive tumors and confirmed the fusion status by RT-PCR and FISH. One RET fusion involved a novel partner, cutlike homeobox 1 gene (CUX1), yielding an in-frame CUX1-RET fusion. ROS1 and RET fusions were significantly enriched in tumors without KRAS/EGFR/ALK alterations. ALK/ROS1/RET/EGFR/KRAS alterations were mutually exclusive. As a single-tube assay, this test shows promise as a more practical and cost-effective screening modality for detecting rare but targetable fusions in NSCLC.

Examining an Effective Communication Message to Promote Participation in Sports Activity: Applying the Extended Parallel Process Model

Lira Yun 글로벌지식마케팅경영학회 2018 Journal of Global Sport Management Vol. No.

The present study examined how a message based on the extended parallel process model can affect individuals’ attitudes and intention to participate in sports activity. A two by two between-subject experiment was conducted among 152 participants who were randomly assigned to one of the four different messages. Individuals’ threat perception was found to increase attitudes and intentions toward sports activity when they also had a high level of efficacy perception to participate in sports activity, supporting the hypothesis. Furthermore, for those with a high level of efficacy, the protective motivation mediated a positive relationship between perceived threat and attitudes, whereas for those with a low level of efficacy, the defensive motivation mediated the negative relationship between perceived threat and attitudes. Neither of the proposed mediators predicted the relationship between perceived threat and intentions. The present study suggests that a theory-based message can help increase campaign effects to promote sports.

The effects of different metal posts, cements, and exposure parameters on cone-beam computed tomography artifacts

Freitas Ana Priscila Lira de Farias,Peixoto Larissa Rangel,Mariz Suassuna Fernanda Clotilde,Bento Patrícia Meira,Maia Amorim Ana Marly Araújo,Rovaris Silva Karla,de Almeida Barros Renata Quirino,Pontu 대한영상치의학회 2023 Imaging Science in Dentistry Vol.53 No.2

Purpose: This study assessed the intensity of artifacts produced by 2 metal posts, 2 cements, and different exposure parameters using 2 cone-beam computed tomography (CBCT) units. Materials and Methods: The sample was composed of 20 single-rooted premolars, divided into 4 groups: Ni-Cr/ zinc phosphate, Ni-Cr/resin cement, Ag-Pd/zinc phosphate, and Ag-Pd/resin cement. Samples were scanned before and after post insertion and cementation using a CS9000 3D scanner with 4 exposure parameters (85/90 kV and 6.3/10 mA) and an i-CAT scanner with 120 kV and 5 mA. The presence of artifacts was assessed subjectively by 2 observers and objectively by a trained observer using ImageJ software. The Mann-Whitney, Wilcoxon, weighted kappa, and chi-square tests were used to assess data at a 95% confidence level (α<0.05). Results: In the subjective analyses, AgPd presented more hypodense and hyperdense lines than NiCr (P<0.05), and more hypodense halos were found using i-CAT (P<0.05) than using CS9000 3D. More hypodense halos, hypodense lines, and hyperdense lines were observed at 10 mA than at 6.3 mA (P<0.05). More hypodense halos were observed at 85 kV than at 90 kV (P<0.05). CS9000 3D presented more hypodense and hyperdense lines than i-CAT (P<0.05). In the objective analyses, AgPd presented higher percentages of hyperdense and hypodense artifacts than NiCr (P<0.05). Zinc phosphate cement presented higher hyperdense artifact percentages on CS9000 3D scans (P<0.05). CS9000 3D presented higher artifact percentages than i-CAT (P<0.05). Conclusion: High-atomic-number alloys, higher tube current, and lower tube voltage may increase the artifacts present in CBCT images.

Frequency and intensity of gastrointestinal symptoms in exercisers individuals at rest and during physical exercise: an internet-based survey

Claudio Andre Barbosa de Lira,Ricardo Borges Viana,Kaylla Priscilla Mesquista,Douglas de Assis Teles Santos,Mário Hebling Campos,Marília Santos Andrade,Rodrigo Luiz Vancini 대한장연구학회 2019 Intestinal Research Vol.17 No.4

Background/Aims: Despite the evidence of a modest to high prevalence of gastrointestinal (GI) symptoms in recreational runners and endurance athletes, the frequency and intensity of GI symptoms in exercisers, but nonathletes, individuals from different modalities have been less investigated. Therefore, the present study aimed to assess the prevalence of GI symptoms in individuals that practice moderate or vigorous physical exercise, at rest and during physical exercise training session. Methods: The sample consisted of 142 exercisers individuals (64 women and 78 men with mean age of 32.9±10.7 years). Out of the 142 participants, 71 reported to perform moderate physical exercise and 71 reported to perform vigorous physical exercise. Participants were assessed by an internet-based questionnaire designed to assess the frequency and intensity (at rest and during physical exercise training session) of 18 GI symptoms. Results: The GI symptoms most frequently reported by the respondents (during rest and physical exercise training session, respectively) were flatulence (90.8% and 69.7%), abdominal noise (77.5% and 41.5%), and eructation (73.9% and 52.1%). Overall, the frequency and intensity of symptoms were higher (P<0.050) during rest than physical exercise training session for who perform moderate and vigorous physical exercise. Conclusions: It can be concluded that GI symptoms in exercisers, but nonathletes, individuals are more prevalent during rest than during physical exercise training session, suggesting that moderate and vigorous physical exercise may act as a regulator of the GI tract.

Gastroprotective Activity of Neoglaziovia variegata (Arruda) Mez. (Bromeliaceae) in Rats and Mice

Kamila Lopes de Lira,Flávia Danniele Frota Machado,Ana Flávia Seraine Custódio Viana,Irisdalva Sousa Oliveira,Francilene Vieira da Silva,Hélio de Barros Fernandes,Jackson Roberto Guedes da Silva Almei 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.10

Neoglaziovia variegata (Arruda) Mez (Bromeliaceae) is a medicinal plant popularly known as “caroá.” The leaves are made up of highly resistant fibers, which is of great commercial value to the handicraft and textile industry. Some studies have demonstrated that ethanolic extract of N. variegata have gastroprotective properties. This study aimed to investigate the gastroprotective activity and cytoprotective mechanisms of ethyl acetate (Nv-AcOEt), hexane (Nv-Hex), and chloroform (Nv-CHCl3) fractions of N. variegata leaves. The gastroprotective activity of Nv-AcOEt, Nv-Hex, and Nv-CHCl3 was evaluated using the ethanol and ethanol/HCl-induced gastric injury model. To elucidate the gastroprotective mechanisms, the functions of prostaglandins (PGs), nitric oxide (NO), and KATP channels were evaluated. In addition, the nonprotein sulfhydryl groups and the mucus content in the gastric tissues were analyzed. All fractions of N. variegata leaves at oral doses of 100, 200, and 400 mg/kg significantly decreased ethanol and ethanol/HCl-induced gastric lesions, leading to gastroprotection, accompanied by an increase in reduced glutathione (GSH) and gastric mucus. Gastroprotective activity of Nv-AcOEt was inhibited after pretreatment with ibuprofen and N(G)-nitro-L-arginine (L-NOARG). Gastroprotective effect of Nv-Hex and Nv-CHCl3 was also inhibited after pretreatment with L-NOARG and with glibenclamide. The results indicate that N. variegata (Arruda) Mez exhibits promising gastroprotective activity with the possible participation of NO, PGs, mucus, sulfhydryl groups, and KATP.

Modulatory Effect of Byrsonima basiloba Extracts on the Mutagenicity of Certain Direct and Indirect-Acting Mutagens in Salmonella typhimurium Assays

Walclecio de Moraes Lira,Fabio Vieira dos Santos,Miriam Sannomiya,Clenilson Martins Rodrigues,Wagner Vilegas,Eliana Aparecida Varanda 한국식품영양과학회 2008 Journal of medicinal food Vol.11 No.1

Byrsonima basiloba A. Juss. species is a native arboreal type from the Brazilian “cerrado” (tropical Americansavanna), and the local population uses it to treat diseases, such as diarrhea and gastric ulcer. It belongs to the Malpighiaceaefamily, and it is commonly known as “murici.” Considering the popular use of B. basilobaderivatives and the lack of phar-macological potential studies regarding this vegetal species, the mutagenic and antimutagenic effect of methanol (MeOH) andchloroform extracts were evaluated by the Ames test, using strains TA97a, TA98, TA100, and TA102 of Salmonella ty-phimurium. No mutagenic activity was observed in any of the extracts. To evaluate the antimutagenic potential, direct and in-direct mutagenic agents were used: 4 nitro-o-phenylenediamine, sodium azide, mitomycin C, aflatoxin B1, benzo[a]pyrene,and hydrogen peroxide. Both the extracts evaluated showed antimutagenic activity, but the highest value of inhibition level(89%) was obtained with the MeOH extract and strain TA100 in the presence of aflatoxin B1. Phytochemical analysis of theextracts revealed the presence of n-alkanes, lupeol, ursolic and oleanolic acid, (. )-catechin, quercetin-3-O-.-L-arabinopyra-noside, gallic acid, methyl gallate, amentoflavone, quercetin, quercetin-3-O-(2.-O-galloyl)-.-D-galactopyranoside, andquercetin-3-O-(2.-O-galloyl)-.-L-arabinopyranoside.

니카라과: 새로운 전환점에 도달했는가?

엘비라 쿠아드라 리라(Elvira Cuadra Lira),임태균(옮김) 서울대학교 라틴아메리카연구소 2019 라틴아메리카이슈 Vol.- No.-

Effect of Angelicae gigantis Radix Extract on Experimentally Induced Hyperlipemia in Rats

Chung, M.-H 한국생약학회 1998 생약학회지 Vol.29 No.4

IMPACTS OF AMBUSH MARKETING: AN EXAMINATION OF THE DANGERS ASSOCIATED WITH IMPLEMENTING AMBUSH MARKETING

Kihan Kim,Lira Yun,Yunjae Cheong 글로벌지식마케팅경영학회 2017 Global Fashion Management Conference Vol.2017 No.07

The number of companies investing substantial marketing resources in sport sponsorship has been growing rapidly. This trend has resulted in more competition among the candidate companies to acquire official sponsorship rights. Not surprisingly, the recent increase in the popularity of sport sponsorship has been accompanied by increased attempts in ambush marketing – a marketing strategy wherein the companies associate themselves with, and therefore capitalize on, a particular sporting event without paying any sponsorship fee (Hoek & Gendall, 2002). The purpose of this study is to examine how such ambush-marketing activities can damage the evaluation of the company when they are noticed by consumers. This study further examines whether the prior reputation of the company influences the relationships between ambush marketing and company evaluations. Sponsorship and Ambush Marketing Prior research in sport marketing (Meenaghan, 1983; Sullivan & Mulphy, 1998) suggests that the sponsorship of sporting events offers sponsors opportunities to develop favorable brand images by creating goodwill perceptions (Hoek, Gendall, and West, 1990; Marshall, 1992). As noted above, companies unable to fund sponsorship rights have attempted to achieve the same benefits by engaging in promotional efforts associated with the event as if they were true sponsors (McKelvey, 1994). It is, however, only when consumers are unaware of the fact that the companies are, in fact, ambush marketers that these companies can expect positive goodwill perceptions from their ambush marketing practices. This is because ambush marketing has been perceived to be an unethical business practice. Payne (1998) indicated that ambush marketing not only harms the integrity and viability of global sport events, but also infringes on official sponsors’ rights; similarly, O’Sullivan and Murphy (1998) claim that ambush marketing is just as bad as stealing. Thus, once consumers become aware of the fact that a company is implementing an ambush marketing practice, rather than being a true sponsor, those consumers will be disappointed, and negative evaluations of the company are most likely to result. Based on the discussion above, the following hypotheses are proposed: H1: When an ambush-marketing attempt is not noticed, subjects exposed to an ambush- marketing practice will have more favorable evaluations of the company than the evaluations of the subjects not exposed to an ambush- marketing practice. H2: When an ambush-marketing attempt is noticed, subjects exposed to an ambushmarketing practice will have more unfavorable evaluations of the company than the evaluations of the subjects not exposed to an ambush- marketing attempt. Expectation and Evidences We further predict that the risks associated with ambush marketing are more profound for companies with high levels of a good reputation, rather than low. Such reasoning is based on theories of the interaction between expectations and evidences. In this study, expectation refers to the belief probabilities of attribute occurrence (Olson and Dover, 1976); prior research suggests that consumers may form expectations about a company based on the corporate reputation—the sum of the values that stakeholders attribute to a company, based on their perceptions of the company’s image and behavior over time (Dalton & Croft, 2003). Corporate reputation comprises three dimensions (Graham and Helen 2005) including public responsibility (e.g., ―Are they socially responsible?‖), consumer fairness (e.g., ―Do they treat the consumers with some respect?‖), and leadership and success (e.g., ―Are they successful at what they do?‖). Thus, having established a high degree of good reputation indicates that consumers can expect more positive perceptions on each of the three dimensions of corporate reputation described above. According to research in consumer satisfaction, it is the direction and the degree of disconfirmation resulting from the incongruity between the prior expectation and the evidences that determine the product evaluation and satisfaction (Oliver 1980). In particular, Oliver (1980), and Tse and Wilton (1988) proposed that expectations are thought to create a frame of reference about which one makes a comparative judgment, whereby evidences higher (or lower) than the expectations are thought to be a positive (or negative) disconfirmation. Mandler (1982) further suggests that increasing the degree of discrepancy between a schema-triggered expectation and new information results in heightened arousal and cognitive processing, which leads to a more polarized evaluation of products and services. Therefore, in a sponsorship context, a seemingly true sponsorship activity (i.e., an unnoticed ambush attempt) is likely to lead to a positive disconfirmation of the consumers’ expectations about a company, and the degree of disconfirmation will be greater when the company’s prior degree of good reputation is low, rather than high. On the other hand, when noticed by consumers, an ambush-marketing attempt is likely to lead to negative disconfirmation of prior expectations about a company, and the degree of disconfirmation will be greater when the company’s prior degree of good reputation is high, rather than low. Based on the discussion, the following hypotheses are posed: H3: When an ambush-marketing attempt is not noticed, subjects exposed to the ambush-marketing practice will perceive a positive disconfirmation of expectations, and the degree of disconfirmation will be greater when the company is disreputable than when it is reputable. H4: When an ambush-marketing attempt is noticed, subjects exposed to the ambush-marketing practice will perceive a negative disconfirmation of expectations, and the degree of disconfirmation will be greater when the company is reputable than when it is disreputable. Disconfirmation and Evaluation Satisfaction literature (Oliver 1980) also suggests a high degree of consistency between the degree of disconfirmation and satisfaction, as well as the traditional criteria of attitudes. Oliver (1980), for example, showed positive relationships between the degree of disconfirmation and the amount of attitude change. Also, given the earlier discussion that the degree of disconfirmation is proportional to the extremity of the attitude, the following hypotheses are posed: H5: When an ambush-marketing attempt is not noticed, subjects exposed to the ambush-marketing practice will have a more favorable attitude toward the company, and the degree of enhancement in company evaluation will be greater when the company is disreputable than when it is reputable. H6: When an ambush-marketing attempt is noticed, subjects exposed to the ambush-marketing practice will have a more negative attitude toward the company, and the degree of reduction in company evaluation will be greater when the company is reputable than when it is disreputable. Method In order to examine the proposed hypotheses, a 2-by-2 by-2 full-factorial experiment will be conducted. A convenient sample of college students will be used for theory-testing purposes. There are three independent variables. Prior reputation (high vs. low reputation), and whether the ambush-marketing attempt is noticed or not (noticed vs. unnoticed) are the between-subject factors, whereas the measurement sequence (before vs. after stimulus exposures) is the within-subject factor. Corporate reputation will be manipulated by exposing subjects to either high- or low-reputation company profiles. Subjects will then be asked to rate the dependent variables the first time. Then, subjects from each company reputation condition will be exposed to hypothetical newspaper articles describing the company as either a true sponsor or an ambush marketer. After being exposed to the stimulus newspaper articles, the subjects will be asked to rate the key dependent variables the second time. The primary dependent variables include perceived corporate reputation, and attitude toward the company. Data collected from the experiment will be analyzed by three-way ANOVA.