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- 저자 : Lingling (검색결과 145 건)
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Lingling Feng,Hui Li,Jingmei Jiao,Ding Li,Li Zhou,Jian Wan,Yangsheng Li 한국식물학회 2009 Journal of Plant Biology Vol.52 No.5
Rice cultivar zhonghua11 (Oryza sativa L. ssp. japonica) plants with decreased sedoheptulose-1, 7- bisphosphatase (SBPase) were obtained by transformation with the rice SBPase antisense gene under the control of the maize ubiquitin promoter. The transgenic and wild-type plants were grown at different nitrogen levels (0.1, 1, or 10 mM NH4NO3). Growth rates of the seedlings were measured by the changes in dry weight, and the photosynthetic carbon reduction activities and the potential efficiency of photosystem II were measured by CO2 assimilation and Fv/Fm, respectively. At low N, there are strong effects on growth and photosynthesis when SBPase was reduced by genetic manipulation. Decreased SBPase activity led to a decrease in the amount of starch accumulated in the leaves at all N levels and the decrease was much more prominent in low N than that in high N, but the starch allocation between shoot and root was unaltered. The analysis of chlorophyll fluorescence and SBPase activity indicated that the decrease of growth and photosynthesis at different N levels were not related to the function of PSII but to the activity of SBPase. Western blot analysis showed the content of SBPase in thylakoid membranes was much more than in the stroma fractions in transgenic plants at low N. Results suggested that low N in addition to a 34% decrease in SBPase activity is sufficient to diminish photosynthesis and limit biomass production. Decreased SBPase activity may reduce the N use efficiency of photosynthesis and growth and alter biomass allocation.
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Lingling Li,Zhenghai Liu,Jiaye Xu,Xuedong Ma 한국전기전자재료학회 2020 Transactions on Electrical and Electronic Material Vol.21 No.3
In order to enhance the segregation eff ect of material particles in a hemispherical shell swing-oscillating trough under combination swing-oscillating, by means of three dimensional discrete element method, and by use of the two-dimensional dry granules of plastic ball and steel ball, the discrete element simulation study of particle segregation process is carried out in turn in three cases, which is composed of a single swing-oscillating in smooth trough, a combination swing-oscillating in smooth trough, and a combination swing-oscillating in adding bulge trough. The particle segregation eff ect was evaluated by particle volume concentration and combined with segregation cloud picture simulated. The result indicates that segregation effect of combination swing-oscillating is better than that in single swing-oscillating; layering eff ect of the trough added bulge is better than that in the smooth trough. And compared to single swing-oscillating in smooth trough, the degree of segregation of particles in added bulge trough can be increased by 10–15% when being combination swing-oscillating.
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Eco-friendly Grafting of Chitosan as a Biopolymer onto Wool Fabrics Using Horseradish Peroxidase
Lingling Xu,Nan Zhang,Qiang Wang,Jiugang Yuan,Yuanyuan Yu,Ping Wang,Xuerong Fan 한국섬유공학회 2019 Fibers and polymers Vol.20 No.2
Chitosan and enzymes have been extensively used in modification of wool, due to their non-toxic and eco-friendly characteristics, as well as to reduce pollution in textile production. In the present work, chitosan was grafted onto wool fabrics using horseradish peroxidase (HRP) in order to endow wool with outstanding properties. The reaction mechanism, grafting percentage, and properties of chitosan-grafted pretreated wool fabric including wettability, dyeability, shrink resistance, and antibacterial activity were studied. P-hydroxyphenylacetamide (PHAD), as a model compound for tyrosine residues in wool,was used to study the mechanism of HRP-mediated grafting of chitosan onto wool. UV and FTIR analyses indicated that quinones, which are intermediate of HRP/H2O2-catalyzed PHAD, reacted with the amino groups of chitosan by Schiff base or Michael addition reactions. Scanning electron microscopy (SEM) showed that dichloroisocyanuric acid pretreatment had an etching effect and some substances were deposited onto the wool fiber. SEM and Fourier transform infrared (FTIR) spectroscopy further confirmed the covalent grafting of chitosan onto wool. Compared with chitosan-grafted unpretreatedwool, there was an obvious improvement in grafting efficiency of chitosan onto wool fabric, physical and mechanical properties after pretreatment and chitosan grafting on the wool. For chitosan-grafted pretreated wool fabrics, there was no observable change in the thermal behavior, while the hydrophilicity, dyeability, shrinkage resistance, and antibacterial activity were remarkably improved compared to that of untreated wool. The present work offers a new ecologically acceptable process of grafting chitosan on the wool.
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Lingling Liu,Shixian Chen,Anchang Xu,Guangming Cai 한국섬유공학회 2020 Fibers and polymers Vol.21 No.2
Adding nano silver wires (AgNWs) onto electrospun polyurethane (PU) nanofiber mat provide a new idea for developing wearable smart textiles. The excellent stretchability of PU and the excellent electrical conductivity of AgNWsenable wearable smart textiles to monitor human physiological data. In this study, PU nanofiber mat was prepared by electrospinning and subsequently was dip into AgNWs dispersion for conductivity. The PU nanofiber mats adsorbed with AgNWs were with both good stretching and electrical conductivity. The surface morphology and surface elements were analyzed by scanning electron microscopy (SEM) and energy dispersive spectrometer (EDS). The mechanical properties and the variation of electrical conductivity under static and dynamic stretching cycles were studied. In addition, it is accurately monitoring variation in electrical conductivity during finger bending and wrist movement. The experimental results show that the PU mat treated by AgNWs has the potential to be used as a wearable strain sensor for real-time monitoring of human motion.
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Lingling Meng 보안공학연구지원센터 2014 International Journal of Hybrid Information Techno Vol.7 No.6
Query suggestion attracts great concern recently. It is crucial for capturing frequently asked questions in question-answering system and most popular topics in search engine. Besides these, is also used in advertising retrieval systems, e-commerce system for advertising push to get more profits. The paper gives a general review of query suggestion methods. On the whole, all the methods can be grouped into two categories: session based methods and click-through based methods. Adjacency based query suggestion, co-occurrence based query suggestion, query-flow graph based query suggestion, clustering based query suggestion, and bipartite graph based query suggestion are presented respectively in detail. Furthermore, how to evaluate the performance of query suggestion is denoted. Finally the important related issues of the area in further research are discussed.
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Lingling Yan,Yiming Bai,Bo Yang,Nuofu Chen,Zhan'ao Tan,Tasawar Hayat,Ahmed Alsaedi 한국물리학회 2018 Current Applied Physics Vol.18 No.4
The efficient photon harvesting in near infrared wavelength range is still a challenging problem for high performance Cu(In1-x, Gax)Se2 (CIGS) solar cell. Herein, adjusting the energy band distribution of CIGS solar cell could provide significant academic guidance for devices with superior output electric power. To understand the role of each functional layer, the optimal 3000 nm CIGS absorber layer with 1.3 eV bandgap and 30 nm CdS buffer layer were firstly obtained via simulating the uniform band-gap structures. By introducing CIGS absorber layer with a double grading Ga/(GaþIn) profile, the power conversion efficiency of the double gradient band gap cell is superior to that of uniform band-gap cell through extending absorption of near-infrared wavelength range. Upon optimization, the best power conversion efficiency of CIGS with a double gradient band gap solar cell is improved significantly to 24.90%, among the best values reported in literatures, which is an 8.17% relative increase compared with that of the uniform band-gap cell. Our findings provide a theoretical guide toward the design of high performance solar cells and enrich the understandings of the energy band engineering for developing of novel semiconductor devices.
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Lingling Deng,Erli Zhang,Chang Chen 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.5
Cisplatin is a platinum-based compound that islargely employed as an effective antitumor drug against awide spectrum of solid neoplasms for many years. Despiteof its initial therapeutic success, cisplatin often results inhigh incidence of chemoresistance and high-dose cytotoxicity. Consequently, considerable efforts in hopes ofreducing the dose-dependent side effects of cisplatin whileretaining, or even enhancing, its antitumor properties havebeen undertaken throughout the past three decades. Nitricoxide (NO) is a small lipophilic free radical gas possessingversatile biological functions, including antitumor activities. However, NO, of itself, is difficult to be used, becauseof its extreme instability and short half-life. Previously, wehave reported a stable NO donor, b-galactosyl-pyrrolidinyldiazeniumdiolate (b-Gal-NONOate), which exerts tumorkilling effects through site-specific intracellular release ofexogenous NO. In this study, we further investigated thecombined inhibitory effect of b-Gal-NONOate and cisplatinagainst C6/LacZ, 9L/LacZ, and HeLa/LacZ tumorcells. It was shown that, in combination with b-Gal-NONOate, the antitumor effects of cisplatin against thesecommon tumor cell lines were increased in a dose-dependentmanner. Furthermore, the combination of thesechemicals resulted in a synergistic suppression on tumorgrowth, which was achieved under a much lower cisplatindosage. Collectively, our findings indicate that b-Gal-NONOate can synergistically improve the antitumor effectof cisplatin, and may therefore reduce its side effectscaused by high dose cisplatin monochemotherapies. Accordingly, b-Gal-NONOate is an important therapeuticassistant reagent with great potential of clinical applicability,and thus worth of continuous research in the comingfuture.
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