Bioinformatic prediction and analysis of glucolipid metabolic regulation by miR-34a in Megalobrama amblycephala

Ling‑Hong Miao,Wen‑Jing Pan,Yan Lin,Bo Liu,Ming‑Chun Ren,Qun‑Lan Zhou,Xian‑Ping Ge 한국유전학회 2017 Genes & Genomics Vol.39 No.12

The objective of this study was to analyze the target genes and regulatory function of miR-34a in Megalobrama amblycephala using second-generation highthroughput sequencing and bioinformatic tools. Functional enrichment analysis was performed by gene ontology. MiR- 34a and target gene expression levels were measured in M. amblycephala fed normal and high-carbohydrate diets. The results revealed that miR-34a was highly conserved in several species, and miR-34a of M. amblycephala has a close evolutionary relationship to that of zebrafish and common carp. miRanda, TargetScan, RNAhybrid predicted 5,185, 6,282 and 2,168 target genes, respectively, and 645 target genes were in common. According to annotation information, the target genes were enriched in phosphate metabolism, glycerophospholipid metabolism, Golgi vesicle transport, cell division, and other biological processes (P < 0.05). Pathway enrichment analysis revealed that these target genes were mainly enriched in alpha-linolenic acid and linoleic acid metabolism, ether lipid metabolism, VEGF signaling pathway, Fc epsilon RI signaling pathway, GnRH signaling pathway, and MAPK signaling pathway (P < 0.05). The regulatory role of miR-34a was more significant in the liver than in the brain of M. amblycephala. MiR-34a regulates glucose lipid homeostasis induced by high glucose diets by upregulating hepatic PI3K/Akt, FOXO, and TOR signaling pathways.

关于中国山东省大数据企业创意役量与组织创新的研究

ling ming Kong,김영길,xi ze Geng 국민대학교 중국인문사회연구소 2020 중국지식네트워크 Vol.16 No.-

Purpose The purpose of this paper is to prove the effect of creative competencies on organizational innovation, and provides a new theoretical basis for organizational innovation research. Design- This paper studies organizational innovation by using creative competencies model and 3M organizational achievement model. Findings - This paper proves that the Portfolio level of the original 5 creative competencies influences the level of organizational innovation of enterprises. The higher the level of organizational innovation, the organizational achievements are higher. Research Implications - In this paper, the proposal of the original 5 creative competencies realizes the concrete, scientific and theoretical connotation of the creative competencies, which provides a powerful theoretical method for the follow-up research. 本文通过关于创意役量与组织创新关系的理论与实证研究,证明了创意役量对组织创新的影响作用,并为组织创新研究提供了全新的理论依据。以创意役量理论和3M组织成果模型为工具研究组织创新问题。本论文证明根源性5创意役量的Portfolio程度影响了企业 的组织创新水平,企业的组织创新水平越高,组织成果就越好。根源性5创意役量的提出实现了创意役量内涵的具体化,科学化,理论化,为后续研究提供了有力的理论支持。

Specification of VRML in Color Rapid Prototyping

Ming, Ling Wai,Gibson, Ian Society for Computational Design and Engineering 2002 International Journal of CAD/CAM Vol.1 No.1

The STL or Stereolithography format, established by 3D systems, gathers the geometric data of the model in a number of triangular surfaces. It can be in ASCII or binary format, and is a de facto standard in the Rapid Prototyping (RP) world. RP has developed greatly over the last ten years. In particular, improvement in materials has meant greater part accuracy and strength, which in turn has increased the range of functional applications. Future applications of RP will focus on rapid tooling and direct manufacturing. Direct manufacturing in particular may see much benefit from the incorporation of color into models. For color RP, besides designing new hardware to add color into the prototypes, it is necessary to redefine the CAD software for adding and accurately positioning color onto the model. STL cannot effectively store this kind of information. Among the existing data file formats, VRML is an acceptable one that is complimentary to existing RP processes. This paper acts as a review to discuss several methods of using VRML for coloring model data. This paper will also discuss the problems occurred in coloring the layer contours of the RP model.

Asparagus Polysaccharide and Gum with Hepatic Artery Embolization Induces Tumor Growth and Inhibits Angiogenesis in an Orthotopic Hepatocellular Carcinoma Model

Weng, Ling-Ling,Xiang, Jian-Feng,Lin, Jin-Bo,Yi, Shang-Hui,Yang, Li-Tao,Li, Yi-Sheng,Zeng, Hao-Tao,Lin, Sheng-Ming,Xin, Dong-Wei,Zhao, Hai-Liang,Qiu, Shu-Qi,Chen, Tao,Zhang, Min-Guang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Liver cancer is one of leading digestive malignancies with high morbidity and mortality. There is an urgent need for the development of novel therapies for this deadly disease. It has been proven that asparagus polysaccharide, one of the most active derivates from the traditional medicine asparagus, possesses notable antitumor properties. However, little is known about the efficacy of asparagus polysaccharide as an adjuvant for liver cancer chemotherapy. Herein, we reported that asparagus polysaccharide and its embolic agent form, asparagus gum, significantly inhibited liver tumor growth with transcatheter arterial chemoembolization (TACE) therapy in an orthotopic hepatocellular carcinoma (HCC) tumor model, while significantly inhibiting angiogenesis and promoting tumor cell apoptosis. Moreover, asparagine gelatinous possessed immunomodulatory functions and showed little toxicity to the host. These results highlight the chemotherapeutic potential of asparagus polysaccharide and warrant a future focus on development as novel chemotherapeutic agent for liver cancer TACE therapy.

Array Sensing Using Differential Magnetic Field Method for Electrode Tip Position of Submerged Arc Furnaces

Wei Ling Liu,Xiao Hong Han,Ling Zhen Yang,Xiao Ming Chang 한국자기학회 2021 Journal of Magnetics Vol.26 No.1

Is It Possible to Replace Microendoscopic Discectomy with Percutaneous Transforaminal Discectomy for Treatment of Lumbar Disc Herniation? A Meta-Analysis Based on Recurrence and Revision Rate

Zhao, Xiao-ming,Yuan, Qi-ling,Liu, Liang,Shi, Ya-ming,Zhang, Yin-gang The Korean Neurosurgical Society 2020 Journal of Korean neurosurgical society Vol.63 No.4

Objectives : Due to recent developments and the wide application of percutaneous transforaminal discectomy (PTED) in China, we herein compare its clinical effects with microendoscopic discectomy (MED) for the treatment of lumbar disc herniation in terms of recurrence and revision rates. Methods : Six databases, namely, PubMed, EMBASE, Cochrane Library, Ovid, China National Knowledge Infrastructure and Wanfang, were searched by computer. The literature was screened according to inclusion and exclusion criteria, and the quality of the included literature was evaluated. After extracting the data from the papers, Review Manager 5.2 software (Cochrane Collaboration, Oxford, UK) was applied to analyze these data. Finally, sensitivity and publication bias analyses of the results were conducted. Results : A total of 12 studies consisting of 2400 patients were included in this meta-analysis. A comparison of PTED with MED revealed higher postoperative recurrence and postoperative revision rates for PTED (odds ratio [OR] recurrence, 1.60; 95% confidence interval [CI], 1.01 to 2.53; p=0.05 and OR revision, 1.77; 95% CI, 1.18 to 2.64, p=0.006). Conclusion : PTED has a number of advantages because it is a minimally invasive surgery, but its recurrence and revision rates are higher than MED. Therefore, MED should not be completely replaced by PTED.

Molecular Cloning and Expression of cDNA Encoding the Cysteine Proteinase Inhibitor from Upland Cotton

Ming-feng Jiang,Sheng-wei Li,Min Chen,Ying-fan Cai,Yong-fang Xie,Biao Li,Quan Sun,Huai-zhong Jiang,Zheng Pan,Yun-ling Gao,You-Lu Yuan,Yu-zheng Shi 한국식물학회 2009 Journal of Plant Biology Vol.52 No.5

A cDNA encoding a novel cysteine proteinase inhibitor (CPI) was isolated from a gland mutant Xiangmian-18 of upland cotton during the pigments gland forming stage. The cDNA comprises 378 bp and encodes 125 amino acid residues with molecular mass of 13.8 kDa. It contains the conserved motif of cysteine protease inhibitors and belongs to the cystatin superfamily (Gln- Val-Val-Ala-Gly). The deduced amino acid sequences of the domains are highly similar to the normal upland cotton (96.8%). SDS-PAGE and western hybridization analysis showed that the expressed recombinant protein was recombinant CPI. The inhibitory activity of recombinant CPI was 46 u/μg which was measured by inhibiting the protease activity of papain. RT-PCR results indicated that the expression level of developing gland stage was higher than that of undeveloped gland stage.

Steel and FRP double-tube confined RAC columns under compression: Comparative study and stress-strain model

Ming-Xiang Xiong,Guangming Chen,Yue-Ling Long,Hairui Cui,Yaoming Liu 국제구조공학회 2022 Steel and Composite Structures, An International J Vol.43 No.2

Recycled aggregate concrete (RAC) is rarely used in load-carrying structural members. To widen its structural application, the compressive behavior of a promising type of composite column, steel-fiber reinforced polymer (FRP) doubletube confined RAC column, has been experimentally and analytically investigated in this study. The objectives are the different performance of such columns from their counterparts using natural aggregate concrete (NAC) and the different mechanisms of the double-tube and single-tube confined concrete. The single-tube confined concrete refers to that in concrete-filled steel tubular (CFST) columns and concrete-filled FRP tubular (CFFT) columns. The test results showed that the use of recycled coarse aggregates (RCA) affected the axial load-strain response in terms of deformation capacity but such effect could be eliminated with the increasing confinement. The composite effect can be triggered by the double confinement of the steel and carbon FRP (CFRP) tubes but not by the steel and polyethylene terephthalate (PET) FRP tubes. The proposed analysis-oriented stress-strain model is capable to capture the load-deformation history of such steel-FRP double-tube confined concrete columns under axial compression.

Study of the Effects of the Antisite Related Defects in Silicon Dioxide of Metal-Oxide-Semiconductor Structure on the Gate Leakage Current

Ling-Feng Mao,Zi-Ou Wang,Ming-Zhen Xu,Chang-Hua Tan 대한전자공학회 2008 Journal of semiconductor technology and science Vol.8 No.2

The effects of the antisite related defects on the electronic structure of silica and the gate leakage current have been investigated using first-principles calculations. Energy levels related to the antisite defects in silicon dioxide have been introduced into the bandgap, which are nearly 2.0 eV from the top of the valence band. Combining with the electronic structures calculated from first-principles simulations, tunneling currents through the silica layer with antisite defects have been calculated. The tunneling current calculations show that the hole tunneling currents assisted by the antisite defects will be dominant at low oxide field whereas the electron direct tunneling current will be dominant at high oxide field. With increased thickness of the defect layer, the threshold point where the hole tunneling current assisted by antisite defects in silica is equal to the electron direct tunneling current extends to higher oxide field.

Relations between rheological and mechanical properties of fiber reinforced mortar

Mingli Cao,Li Li,Ling Xu 사단법인 한국계산역학회 2017 Computers and Concrete, An International Journal Vol.20 No.4

Fresh and hardened behaviors of a new hybrid fiber (steel fiber, polyvinyl alcohol fiber and calcium carbonate whisker) reinforced cementitious composites (HyFRCC) with admixtures (fly ash, silica fume and water reducer) have been studied. Within the limitations of the equipment and testing program, it is illustrated that the rheological properties of the new HyFRCC conform to the modified Bingham model. The relations between flow spread and yield stress as well as flow rate and plastic viscosity both conform well with negative exponent correlation, justifying that slump flow and flow rate test can be applied to replace the other two as simple rheology measurement and control method in jobsite. In addition, for the new HyFRCC with fly ash and water reducer, the mathematical model between the rheological and mechanical properties conform well with the quadratic function, and these quadratic function curves are always concave upward. Based on mathematical analysis, an optimal range of rheology/ flowability can be identified to achieve ideal mechanical properties. In addition, this optimization method can be extended to PVA fiber reinforced cement-based composites.