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Ling‑Hong Miao,Wen‑Jing Pan,Yan Lin,Bo Liu,Ming‑Chun Ren,Qun‑Lan Zhou,Xian‑Ping Ge 한국유전학회 2017 Genes & Genomics Vol.39 No.12
The objective of this study was to analyze the target genes and regulatory function of miR-34a in Megalobrama amblycephala using second-generation highthroughput sequencing and bioinformatic tools. Functional enrichment analysis was performed by gene ontology. MiR- 34a and target gene expression levels were measured in M. amblycephala fed normal and high-carbohydrate diets. The results revealed that miR-34a was highly conserved in several species, and miR-34a of M. amblycephala has a close evolutionary relationship to that of zebrafish and common carp. miRanda, TargetScan, RNAhybrid predicted 5,185, 6,282 and 2,168 target genes, respectively, and 645 target genes were in common. According to annotation information, the target genes were enriched in phosphate metabolism, glycerophospholipid metabolism, Golgi vesicle transport, cell division, and other biological processes (P < 0.05). Pathway enrichment analysis revealed that these target genes were mainly enriched in alpha-linolenic acid and linoleic acid metabolism, ether lipid metabolism, VEGF signaling pathway, Fc epsilon RI signaling pathway, GnRH signaling pathway, and MAPK signaling pathway (P < 0.05). The regulatory role of miR-34a was more significant in the liver than in the brain of M. amblycephala. MiR-34a regulates glucose lipid homeostasis induced by high glucose diets by upregulating hepatic PI3K/Akt, FOXO, and TOR signaling pathways.
Ling-Ling Xie,Yuan-Dong Xu,Jie-Jie Zhang,Cheng-Peng Zhang,Xiao-Yu Cao,Ling-Bo Qu 대한금속·재료학회 2013 ELECTRONIC MATERIALS LETTERS Vol.9 No.4
Er-doped LiV3O8 as cathode material for secondary lithium batteries was prepared through a rheological phase reaction method. The as-prepared materials were investigated by x-ray diffraction (XRD), scanning electron microscopy (SEM), and galvanostatic discharge/charge measurements. The results indicate that Er doped phase preserves the layered structure of the pristine LiV3O8 and has an enlarged interlayer spacing. Compared to LiV3O8 sample, Er-doped LiV3O8 sample displays more uniform particles and large surface area. The electrochemical test shows that Er doping does not change the process of Li+ insertion/deinsertion. Er-doped LiV3O8 electrode exhibits an initial discharge capacity of 294.2 mAh g−1 and maintains a stable capacity of 220.7 mAh g−1 after 50 cycles, indicating a greatly improved good cycleability comparing with the undoped one.
Ling-yan Li,Shuang-lin Li,Yan Shao,Rui Dou,Bo Yin,Ming-bo Yang 한국물리학회 2018 Current Applied Physics Vol.18 No.4
In this work, Polyvinylidene Fluoride (PVDF)/polystyrene (PS)/high density polyethylene (HDPE) ternary blends displayed a core-shell structure where HDPE was the core, PS was the shell, and this core-shell system dispersed in PVDF matrix. Here, multiwall carbon nanotubes (MWCNTs) and ferroferric oxide (Fe3O4) was incorporated. F-F composites with MWCNTs was in PS shell and Fe3O4 was in PVDF matrix and E-F composites with MWCNTs was in PS shell and Fe3O4 was in HDPE core were fabricated by melt blending. It was indicated that the core-shell morphology between PS and HDPE was well retained with the incorporation of Fe3O4 and MWCNTs. Both the electrical conductivity of F-F and E-F composites were similar without no obvious change with the incorporation of Fe3O4. Composites with greater than 20 dB shielding effectiveness were easy to obtain. The highest SE we observed was for the F-F composite with 1 vol% Fe3O4 and 1 vol%MWCNTs was 25 dB at 9.5 GHz, and the SE was over 20 dB in the whole measured frequency(X-band). The E-F composites with SE greater than 20 dB in X-band was at 2 vol% Fe3O4 and 1 vol% MWNCTs. Such effective and lightweight nanocomposites were obtained, resulting from the synergetic effect of MWCNTs and Fe3O4 nanoparticles.
Ling-Ling Xie,Xiao-Yu Cao,Li-Xu Zhang,Zhong-Xu Dai,Ling-Bo Qu 대한금속·재료학회 2013 ELECTRONIC MATERIALS LETTERS Vol.9 No.2
A LiV3O8/polyaniline (PAn) composite was prepared by the in-situ polymerization method assisted by sodium dodecyl sulfate and ammonium persulfate. The as-prepared powders were investigated by XRD, SEM, and galvanostatic discharge/charge analysis. It was found that the introduction of PAn to LiV3O8 can effectively buffer the mechanical stress and restrain the number of phase changes of composite material during the electrochemical cycling. Compared with pristine LiV3O8, LiV3O8/PAn composite maintains a reversible capacity of 212.1 mAh g−1 at the current density of 30 mA g−1 after 50 cycles, approximately 22.6%, much higher than the former.
Co3(PO4)2-Coated LiV3O8 as Positive Materials for Rechargeable Lithium Batteries
Ling-Ling Xie,Li-Qin You,Xiao-Yu Cao,Chao-Feng Zhang,Da-Wei Song,Ling-Bo Qu 대한금속·재료학회 2012 ELECTRONIC MATERIALS LETTERS Vol.8 No.4
Co3(PO4)2-coated LiV3O8 has been successfully synthesized and used as positive material for rechargeable lithium batteries by a facile liquid phase method. The as-prepared powders were characterized by x-ray diffraction (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM), and the galvanostatic discharge/charge experiments. As-prepared Co3(PO4)2-coated LiV3O8 forms a good layered structure with a poor cyrstallinity. SEM reveals that Co3(PO4)2-coated LiV3O8 has uniform particle distribution and reduced particle size when compared with bare one. The Co3(PO4)2 coating layer is about 33 - 59 nm forming a continuous lumps attached to LiV3O8 particle surface. Co3(PO4)2-coated LiV3O8 electrode shows increased capacity and more stable cycling. The first and 35th discharge capacities of the Co3(PO4)2-coated LiV3O8electrode are 322.8 mAh g−1 and 235.7 mAh g−1 in the range of 4.0 - 1.8 V at a current rate of 30 mA g−1, respectively. The improved electrochemical performance is assigned to the greatly reduced LiV3O8 particle with uniform morphology. Co3(PO4)2-coating further benefits the phase transitions of LiV3O8 during discharge/charge while preventing parasite reactions between electrode surface and electrolyte.
Weng, Ling-Ling,Xiang, Jian-Feng,Lin, Jin-Bo,Yi, Shang-Hui,Yang, Li-Tao,Li, Yi-Sheng,Zeng, Hao-Tao,Lin, Sheng-Ming,Xin, Dong-Wei,Zhao, Hai-Liang,Qiu, Shu-Qi,Chen, Tao,Zhang, Min-Guang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24
Liver cancer is one of leading digestive malignancies with high morbidity and mortality. There is an urgent need for the development of novel therapies for this deadly disease. It has been proven that asparagus polysaccharide, one of the most active derivates from the traditional medicine asparagus, possesses notable antitumor properties. However, little is known about the efficacy of asparagus polysaccharide as an adjuvant for liver cancer chemotherapy. Herein, we reported that asparagus polysaccharide and its embolic agent form, asparagus gum, significantly inhibited liver tumor growth with transcatheter arterial chemoembolization (TACE) therapy in an orthotopic hepatocellular carcinoma (HCC) tumor model, while significantly inhibiting angiogenesis and promoting tumor cell apoptosis. Moreover, asparagine gelatinous possessed immunomodulatory functions and showed little toxicity to the host. These results highlight the chemotherapeutic potential of asparagus polysaccharide and warrant a future focus on development as novel chemotherapeutic agent for liver cancer TACE therapy.
Ling-Li Sun,He-he Liu,Hao-han Wang,Jian-Ming Si,Hai-bo Jin,Xin-xin Li,Chao Yang,Liang Li,Jiwen Wang 한국유전학회 2013 Genes & Genomics Vol.35 No.3
Myogenic enhancer transcription factor 2c (MEF2c), one of the members of the MEF2 family of transcription factors, plays an important role in mammalian muscle development. However, the role of MEF2c in avian muscle development still remains unclear. To understand the function of MEF2c in avian muscle development, we first cloned the duck MEF2c coding domain sequence (CDS) and analyzed MEF2c expression in duck muscle tissues of embryos from 10 days of incubation to 1 week after birth using real-time PCR technology. The results showed that the duck MEF2c CDS consists of 1,398nucleotides that encode 465 amino acids. The MEF2c duck protein contains a MADS domain, a MEF2 domain and a HJURP_C domain with high homology to related proteins in other organisms. Different expression levels of MEF2c were found in skeletal, smooth and cardiac muscle. Therefore, these results indicated that duck MEF2c has two conserved domains (a MADS and a MEF2 domain), is an indispensable regulator of muscle development, and plays an important role in the development of duck muscle.