In-vitro and In-vivo Anti-hyperglycemic Effects of Nipa palm (Nypa fruticans Wurmb) Extract

Kyung-Tae Kwon,Gi Jung Kim,Tae Yang Kim,Jin-A Yu,Su-Young Lee,Ji-Yoon Hong,Ye Lim Jang,Tessema Haimanot Miticu,Young-In Kwon 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

In this study, Nipa palm extract by solvent were investigated for their anti-hyperglycemic and using in-vitro, in-vivo animal models. In-vitro study, we investigated the inhibitory activities of Nipa palm stems and flower extracts by solvent against various digestive enzymes such as α-glucosidase, sucrase, maltase, and glucoamylase. In addition, α-amylase inhibitory activity, oxygen radical absorbance capacity, total phenolic and flavonoid content were measured. Among solvent extract, stem water extract (SWE) had the highest α-glucosidase inhibitory activity (3.6 mg/ml of IC50) and high sucrase inhibitory activity (0.28 mg/ml IC50). Furthermore, the extracts were investigated for in-vivo post-prandial blood anti-hyperglycemic effect using Sprague-Dawley(SD) rat model. The maximum blood glucose concentration(Cmax) in SWE-treated SD-rat (0.5 g-ΚBE/kg-bw) was significantly lower than in untreated SD-rats (199.9±10.4 vs. 222.0±15.4 hr·mg/dL, p<0.05). These results indicate that SWE may improve exaggerated post-prandial spikes in blood glucose via inhibition of intestinal α-glucosidase and thus delays carbohydrate absorption in colon.

대도시 지역주민의 흡연실태와 관련요인 분석

김기열,임상규,서인선,강창열,이병직,남철현,위광복 慶山大學校 保健福祉硏究所 1997 保健福祉硏究 Vol.1 No.-

To collect basic data for deveoping education program for quitting smoking, 1200 subjects were selected randomly living in Seoul, Pusan, Taegu and suburban areas and interviewed about their smoking habits and attitudes from June 20 to July 20, 1997. The result of this study was summarized as follows: 1. In the general characterisics of subjects, the proportion of female was the higher(53.8%) than that of male. Among them, the portion of twenties was 46.6%. in level of educational background, the rate of college graduates was 45.2%, that of high school was 35.7%, that of middle school was 11.5%. The highest proportion by occupation was 38.9% of student. The next was 11.7% of technician, 10.1% of unemployed, 9.4% of office worker and 9.3% of salesman. 2. The proportion of the smokers among subjects was 34.3%. The portion of male smokers was 65.7%, that of female smokers was 7.3%, which had tendency to increased. At the rate of smoking by age, 47.6% in over fifties, 43.6% in forties and 38.7% in thirties were showed that the rate of smoking was the higher in the older age. 3. In the quantity of smoking cigarettes, the rate of one park of cigarettes per day was 52.7%, that of a half pack was 31.1% and that of over two 53.2% and that in female was 59.1% which was highest. 4. In smoking period, the rate of smokers for over 20 years was 29.7%, that for 1-4 years was 25.1% and that for 5-9 years was 20.9%. The variables of age, marital status, educational level and occupation were statistically significant(p<0.01). 5. In the intention of quitting smoking, 48.0% of subjects wanted to stop smoking, 33.4% of them wanted to reduce amounts of cigarettes and 82.8% fo them had positive intention to stop smoking, on the other side 18.6% of subjects did not have any intention to stop smoking. 6. The proportion of subjects who tried to stop smoking was 67.9%. By characteristics, the rate of 67.9% in male, 27.3% in female, 90.9% in under nineteens, 73.6% in married group 69.4% in lower social class, 77.8% in high school graduated and 72.3% in Buddist was the higher than that of other groups. 7. The proportion of subjects who succeeded in quitting smoking was 12.0%. By characteristics, the rate of 40.2% in male, 34.8% in over 50 years old, 19.7% in married group, 15.4% in lower social class, 13.9% in apartment resident, 15.7% in high school graduate and 17.1% in Buddist was the higher than that of other group. 8. In the reason of quitting smoking, 81.0% of subjects had quitted smoking for health, 6.0% of them for religion and 13.0% of them for others. By characteristics, the rate of reason for health was 83.5% in male, 100.0% in age of forties, 84.6% in lower social class. 9. In the affecting factors of quitting smoking, 40.7% of subjects was influenced on family which was the highest, 29.6% of them on health education, 14.8% of them on friend's recommandation, 9.9% of them on medical doctor's advice and 4.9% of them on campaign. By age, twenties was affected by friend, thirties by health education and forties by family. 10. The most affecting independent variable of smoking was sexuality (β=0.42055), age(β=0.25285), drinking (β=0.15425), marital status and health condition(R²=44.8%). These results were shown that education program for quitting smoking especially emphasized injurious to health should be continued in the unit of family, school, office and society, particularly education program for female should be strengthened.

대학생의 노인복지에 대한 인식도

차병준,김무룡,김기열,차경미,김귀희,김현실,서인선,임상규,이순자,위광복,남철현 慶山大學校 保健福祉硏究所 1999 保健福祉硏究 Vol.2 No.-

The purpose of this study is to grasp the change of consciousness of young people in order to seek new approach of policy for aged era. 1,200 students attending collages and universities in three small or medium sized cities were inquired for 2 months, from October 1 to November 30, 1996. The result is as follows. 1. The characteristic of the respondents of this survey is that they include 72.1% of women, 40.4% of people aged between 20 to 21, and 49.1% of people who do not have any religion. Those who are from big cities and those who are form farming villages or fishing village occupy equally 40.2%, those who reside in that cities for a long period 49.0%, and those who live with their family 60.9%. 2. The family feature of the subject is that 31.5% of them are those whose father is the age of 55 and more than 55, 10% of them are those whose mothers age is so, 33.1% of them have 5 family members, 29.5% of them have the family income of 1,000,000 to 1,490,000 won, 83.9% of them are from middle class, 47.7% of them are the people whose grand parents have passed away, 70.4% of them live apart from their grand parents, and 60.9% of them are second child. 3. 64.0% of males, 70% of respondents who are aged over 24, 58.1% of respondents who are from rural communities, 62.4% of respondents who live in rural area, and 58.3% of respondents who live alone, prefer to live with grand parents, marked higher rate than other groups. The groups whose father and mother are the age of more than 55 marked higher rate of 55.9% and 58% each than any other groups(p<0.05). The groups whose family member is less than three, whose grand parents both have passed away in middle class, who live with their grand parents, and who are second children, give more positive answers than any other groups. 4. Those who are older, who are buddhists, who are from rural area or live in rural area, whose family is small, whose family income is small, who are from lower class and whose grand parents are still alive, shows preference to support their parents after marriage. 5. Females(89.4%), those who are from small or medium sized cities(89%), who live with their family(85.6%), whose father is the age of 55 and more than 55, whose mother is the age of 54 and less than 54, whose family member is 6, who are from middle class, whose grandparents are still alive and who are the eldest child in their family, give more response that they prefer to live apart from their children in their old age than any other groups. 6. What elderly people need most right now is past time(42.3%), and the respondents whose age is 20 to 21, who are buddhists, who are from middle sized cities and live alone, whose parents are the age of more than 55, who have small family income, who live with their grand parents and who are second children show higher level than any other groups. 7. 76.8% of respondents answer that they do not need their parent's fortune, marked still higher rate than the opposite answer. Those who are older, who are buddhists, who are from big cities, who reside in rural area, who live alone and have smaller family, who are in bad family economic conditions and who live with grand parents present higher rate of positive response. 8. 59.3% of respondents reply that they think about death from time to time, and those who are female, who are in the age of 20 to 21, who are christian, who live with their family, whose siblings or other family members offer their education expenses show higher rate than any other groups. 9. 92.3% of respondents answer that the budget for welfare facility for elderly people is very small. The younger the respondents are and the worse economic condition they have among those who live in big cities, the more answered that. 10. 50% of respondents think social security should provide for their old age, and 42.8% of them believe they themselves provide against it. The respondence that social security ought provide for it appeared more among those whose age are over 24, who are female, who reside in rural area, who are christian, who are in bad economic condition, and who ever used medical institutions in recent one month, marked higher level than other groups. 11. The adequate time of retirement is 59.97±5.60 for public officers, the highest and 59.28±6.35 for teachers, the next. 12. 35.2% of respondents reply that the adequate budget for the old age after their retirement at the age of 60 is 220 million won, showed the highest level of respondence. 13. The factors which affect on the old people in the charged welfare facilities are sex(p<0.01), the type of housing(p<0.05) and whole family income(p<0.05). 14. The factors which affect on remarriage are sex(p<0.05) and religion(p<0.05). For varying old population and expanded welfare service for them, government and relevant authority should give more attention to secure the budget, establish effective plans to expand various program and the facilities for elderly people in order that elderly people spend the rest of life time more happily and more fruitfully. To do that, each college and university should open Health courses, and educate and inform about health and welfare.

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

이차구순열 결손의 치료

임석균,박인순,여환호,지재휴,김영균,김수관 大韓顎顔面成形再建外科學會 1997 Maxillofacial Plastic Reconstructive Surgery Vol.19 No.2

Despite the current accomplishments with the repair of cleft lips, the surgical management of the nasal deformity remains a functional and aesthetic dilemma for patients, their families, and reconstructive surgeons. Recent improvements in the understanding and technical execution of te primary cleft lip repair have significantly reduced secondary sequelae and the consequent need for secondary surgical correction. But, secondary surgical corrections are necessary according to numerous factors. Such factors include the secondary surgical corrections are necessary according to numerous factors. Such factors include the severity of the initial deformity, the surgical plan, precision of execution of the primary repair, and success of the postoperative management. We preformed the secondary correction of cleft lip and palate in 11 patients via various methods. In conclusion, primary repair of cleft lip and palate patients is the most important to prevent the secondary deformities, and most of cleft lip and palate with secondary deformities must be treated with combined cheiloplasty and rhinoplasty.

Effect of Long-term Administration of Jeju barley (Hordeum vulgare) Extract on Weight Management in In-vivo Animal Model

Su-Young Lee,Ji-Yoon Hong,Tae Yang Kim,Jin-A Yu,Gi Jung Kim,Ye Lim Jang,Tessema Haimanot Miticu,Jung Bae Oh,Young-In Kwon 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

In this study, the effect of long-term intake of Jeju barley extract (JBE) on the anti-obesity was evaluated using diet-induced obesity C57BL/6 mouse model. The animal experiment was conducted for 8 weeks, and a total of 20 mice were divided into 4 groups: a normal diet group (ND), high-fat diet group (HFD), barley 0.3 g/kg group (BL) and barley 0.5 g/kg group (BH). The JBE administration group was fed a high-fat diet and the sample was orally administered twice a day. As a result of measuring the change in body weight every other day, it was confirmed that the BL and BH group showed less weight gain than the HFD group. After sacrifice, various adipose tissue weights, lipid levels {total cholesterol (TC), triglycerides (TG), HDL-Cholesterol (HDL-C), LDL-Cholesterol (LDL-C)}, adiponectin, leptin, TNF-α, IL-6 and insulin were measured. TC, TG and LDL-C levels were significantly lower in the BL group than in the HFD group. It was also confirmed that the leptin level was significantly lower than that of the HFD group. These results indicate that proper intake of JBE may reduce weight gain and improve obesity-related indicators such as adiponectin, leptin, TC, TG and HDL-C levels.

흰쥐 Kupffer 세포에 대한 Propofol의 작용

박세훈,지대림,성언기,김희선,송인환,박희영,김준수,이덕희,박대팔 대한마취과학회 2002 Korean Journal of Anesthesiology Vol.43 No.4

Background: Propofol has an antioxidant capacity and can be used for ischemia-reperfusion injury of the liver. However, the effects of propofol on the Kupffer cells have not been establisked. Methods: Kupffer cells were isolated and cultured from male Sprague-Dawley rats. The effects of propofol on the Kupffer cells were evaluated by a phagocytosis assay, TNF-α gene expression, and superoxide anion release after administering propofol in different concentrations on the cultured Kuprrer cells. Results: The latex bead phagocytosis by the Kupffer cells was suppressed when the Kupffer cells were exposed to propofol irrespective of concentrations. Higher propofol concentrations decreased the loss of Kupffer cells after latex bead phagocytosis. Propofol induced TNF-α mRNA expression in the Kupffer cells, but the mRNA expression level after 50㎍/ml of propofol decreased. The pattern of TNF-α mRNA expression induced by propofol was different to that induced by LPS: TNF-α mRNA was expressed continuously in the propofol-treated cells until 16 hours after exposure to propofol, whereas the level of TNF-α mRNA expression induced by LPS was evident after 2 hours and was not found thereafter. TNF-α production after propofol treatment was not higher than that of the control. Formazan presipitation did not show any qualitative differences between cells untreated or treated with propofol concentrations of 0.5, 5.0, and 50 ㎍/ml. Conclusions: These results showed that propofol might inhibit Kupffer cells. This suggests that propofol and be used for patients with ischemia-reperfusion injury of the liver. (Korean J Anesthesiol 2002; 43: 475~484)

Usefulness of Pulsatile Flow Aortic Aneurysm Phantoms for Stent-graft Placement

Tae-Hyung Kim(김태형),Gi-Young Ko(고기영),Ho-Young Song(송호영),In Kook Park(박인국),Ji Hoon Shin(신지훈),Jin-Oh Lim(임진오),Jin Hyoung Kim(김진형),Eugene K. Choi 대한방사선과학회(구 대한방사선기술학회) 2007 방사선기술과학 Vol.30 No.3

  대동맥류 질환에 사용되는 스텐트그라프트의 체외실험을 위한 대동맥류 혈류 팬텀의 유효성과 실현가능성에 대해 평가하고자 한다. 팬텀은 인체의 혈류 조건과 유사한 상황을 재현할 수 있도록 심장부분과 대동맥류 부분으로 구성되었다. 심장부분은 고압력 수중펌프와 솔레노이드 밸브를 사용하여 심장의 대동맥 혈류를 재현하였고, 대동맥류 부분은 지점토를 사용하여 동맥류 모양을 재현하고 그 틀을 투명 실리콘으로 틀을 떠내는 방법으로 제작하였다. 두부분은 실리콘 관으로 연결하였다. 제작된 팬텀에서 밸브의 개폐 시간에 따른 압력(수축기/이완기) 변화를 측정하였으며, 스텐트그라프삽입술 전, 후의 압력변화를 측정하였으며, 통계적 유의성을 알아 보았다. 밸브의 개폐 시간에 따른 압력 변화는 통계적으로 유의한 결과를 보였다(P <0.05). 0.5회/초의 개폐 조건에서는 팬텀의 대동맥 근위부, 대동맥류, 원위부의 압력은 각각 157.80±1.92/130.20±1.92, 159.40±1.14/134.00±2.92, 147.20±1.480/129.60±2.70mmHg이었으며, 1.0회/초의 개폐 조건에서는 161.40±1.34/90.20±1.64, 175.00±1.58/93.00±1.58, 176.80±1.48/90.80±1.92mmHg이었고, 1.5회/초의 개폐 조건에서는 159.40±1.82/127.20±1.48, 166.60±1.67/138.00±1.87, and 161.00±1.22/135.40± 1.67mmHg이었다. 스텐트 그라프삽입술 전, 후의 팬텀의 압력변화는 대동맥부분에서 측정하였으며, 각각 143.60±1.67/90.20±1.64, 147.20±1.92/84.60±1.82, and 137.40±1.52/88.80±1.64mmHg이었다. 결론적으로, 대동맥류 팬텀은 압력의 범위를 다양하게 적용할 수 있고, 팬텀 내에서 시술의 재현이 가능하여 동물실험 전 스텐트그라프트의 유용성을 평가하기 위한 체외실험 기구로 유용할 것으로 기대된다.   To evaluate the feasibility and efficacy of a pulsatile aortic aneurysm phantoms for in-vitro study.<BR>  The phantoms consisted of a pulsating motor part(heart part) and an aortic aneurysm part, which mimicked true physiologic conditions. The heart part was created from a high-pressured water pump and a pulsatile flow solenoid valve for the simulation of aortic flow. The aortic aneurysm part was manufactured from paper clay, which was placed inside a acrylic plastic square box, where liquid silicone was poured. After the silicone was formed, the clay was removed, and a silicone tube was used to connect the heart and aneurysm part. We measured the change in pressure as related to the opening time(pulse rate, Kruskal-Wallis method) and pressure before and after the stent-graft implantation(n=5, Wilcoxon"s signed ranks test).<BR>  The changes in blood pressures according to pulse rate were all statistically significant(p <0.05). The systolic/diastolic pressures at the proximal aorta, the aortic aneurysm, and the distal aorta of the model were 157.80±1.92/130.20±1.92, 159.40±1.14/134.00±2.92, and 147.20±1.480/129.60±2.70mmHg, respectively, when the pulse rate was 0.5 beat/second. The pressures changed to 161.40±1.34/90.20±1.64, 175.00±1.58/93.00±1.58, and 176.80±1.48/90.80±1.92mmHg, respectively, when the pulse rate was 1.0 beat/second, and 159.40±1.82/127.20±1.48, 166.60±1.67/138.00±1.87, and 161.00±1.22/135.40±1.67 mmHg, respectively, when it was 1.5 beat/second. When pulse rate was set at 1.0 beat/second, the pressures were 143.60±1.67/90.20±1.64, 147.20±1.92/84.60±1.82, and 137.40±1.52/88.80±1.64mmHg after stent-graft implantation. The changes of pressure before and after stent-graft implantation were statistically significant(p <0.05) except the diastolic pressures at the proximal(p =1.00) and distal aorta(p = 0.157).<BR>  The aortic aneurysm phantoms seems to be useful for the evaluation of the efficacy of stent-graft before animal or clinical studies because of its easy reproducibility and ability to display a wide range of pressures.

In-vitro and In-vivo Anti-hyperglycemic Effects of Nipa palm (Nypa fruticans Wurmb.) Extracts

Su-Young Lee,Kyung-Tae Kwon,Gi Jung Kim,Ji-Yoon Hong,Ye Lim Jang,Young-In Kwon 한국식품영양과학회 2022 한국식품영양과학회 학술대회발표집 Vol.2022 No.10

Evaluation of In-vitro Anti-diabetic Effects of Arginyl-Fructose Enriched Korea Red Ginseng (Panax ginseng Meyer) Extract

Ye Lim Jang,Gi Jung Kim,Ji-Yoon Hong,Cheong-Weon Cho,Byung-Ryul Sun,Young-In Kwon 한국식품영양과학회 2022 한국식품영양과학회 학술대회발표집 Vol.2022 No.10