Flavonoid Compounds from the Leaves of Kalanchoe prolifera and Their Cytotoxic Activity against P-388 Murine Leukimia Cells

Lilis Siti Aisyah,Yenny Febriani Yun,Tati Herlina,Euis Julaeha,Achmad Zainuddin,Ida Nurfarida,Ace Tatang Hidayat,Unang Supratman,Yoshihito Shiono 한국생약학회 2017 Natural Product Sciences Vol.23 No.2

Seven flavonoid compounds, kaempferol (1), quercetin (2), quercetin-3-O-b-D-glucopyranoside (3), kaempferol-3-O-b-D-glucopyranoside (4), kaempferol-3-O-a-L-rhamnoside (5), quercetin-3-O-sophoroside (6) and quercetin-3-O-rutinoside (7), were isolated from the methanolic extract of leaves of Kalanchoe prolifera. Compounds 1-7 were isolated for first time from this plant. These compounds were evaluated their cytotoxic activity against P-388 murine leukimia cells in vitro. Among those compounds kaempferol (1) and quercetin (2) showed strongest cytotoxic activity with IC50 values of 4.45 ? 0.05 and 6.28 ? 0.02 mg/mL, respectively.

Decreasing Return to Scale in Cottage Industries: Empirical Evidence from the Coconut Sugar Industry in Banyumas, Indonesia

BADRIAH, Lilis Siti,ARINTOKO, Arintoko,RAHAJUNI, Dijan Korea Distribution Science Association 2022 The Journal of Asian Finance, Economics and Busine Vol.9 No.7

This study aims to analyze the economies of scale of the cottage industry for coconut sugar production in Banyumas, Indonesia. This study applies a survey method to coconut sugar craftsmen. Data analysis was performed by regression analysis based on the Cobb-Douglas production function approach. The findings indicated that decreasing returns to scale was a problem for the domestic production of coconut sugar. These findings show that the production of coconut sugar is not very productive. Labor and financial capital inputs have a significant positive share but the resulting increase in output is less proportional than the increase in the two inputs. Social capital, experience, and education do not affect industrial performance. To increase input productivity and production efficiency, it is necessary to apply more effective production techniques and technologies to produce quality products so that the selling price can be higher. Additionally, it is required to enhance the sap's quality through its extraction methods and the regrowth of high-yielding coconut tree varieties. From the institutional aspect, the development of this industry requires stronger partnerships with related parties such as local governments, exporting companies, cooperatives, and universities through research and development.

THE EFFECTIVENESS OF GOVERNMENT PROGRAMME ON INTENTION TO REPATRIATE: A CASE OF MALAYSIAN SELF - INITIATED EXPATRIATES.

( Siti Fatimah ),( Lilis Surienty ),( Yusliza Mohd Yusoff ) 한국정책학회 2016 한국정책학회 추계학술발표논문집 Vol.2016 No.-

The issue of brain drain was the basis for this study. Countries, especially developing like Malaysia, have felt insecure when their talented professinals show an outflow movement out of the country. Losing this skilled workforee will bring difficulties for the home country in achieving high per capita income thus undermining the economic development. In every nation, the government play an important role to create programmes and policy in order to attract talent. As for that, Malaysian government hired TalentCorp alaysia Berhad (TalentCorp) to develop strategies and implement more effective action plan to alleviate the brain drain issues in Malaysia. Returning Expert Programme(REP) was one of the program introduced to attract and facilitate the return of experienced Malaysian Professionals from abroad. Due to that, this article aims to add knowledge on the effectiveness of government programme and how it influences the intention to repatriate among self-initiated expatriates (SIEs). A total of 130 responses were gathered from Malaysian self-initiated expatriates who are currently abroad. The data were then analysed The data were then analysed using structural equation modelling to confirm the proposed relationship. The results showed that government programme was not the causal factors of the SIE`s inention to repatriate. Finding indicate that the government has only a limited control over the expatriate`s decision. However, the government play a role in providing incentives or aid of repatriation for the self-initiated expatriates to ease the repatriation process. Thus, the implication of the findings was pres ented and recommendation were made for future studies.

In Vitro Characterization of Lactic Acid Bacteria from Indonesian Kefir Grains as Probiotics with Cholesterol-Lowering Effect

Dandy Yusuf,Lilis Nuraida,Ratih Dewanti-Hariyadi,Dase Hunaefi 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.5

Indonesian kefir grains are potential sources of lactic acid bacteria (LAB) that may act as probiotics with specific functional properties. In this study we explored the potential of the probiotic and cholesterol-lowering effect of LAB isolated from Indonesian kefir grains obtained from Bogor, Bandung, Jakarta, and Yogyakarta. The results revealed that 10 isolates showed considerable survivability at low pH and bile salt with total cell reduction of ~3 log colony-forming units per milliliter after exposure to pH 2.5 and 0.5% (w/v) bile salt for 1 and 3 h, respectively. All strains exhibited strong antimicrobial activities against pathogenic bacteria and were sensitive to a wide spectrum of antibiotics but exhibited weak bile salt hydrolase activity. Identification based on 16S RNA suggested that nine isolates were Lactobacillus kefiri and one was Lactobacillus rhamnosus. The ability of the isolates to reduce cholesterol from the media varied, ranging from 22.08% to 68.75% with the highest reduction shown by L. kefiri JK17. The ability to remove cholesterol from the media decreased greatly in resting and dead cells, ranging from 14.58% to 22.08% in resting cells and from 7.89% to 18.17% in dead cells. It can be concluded that Indonesian kefir grains contain LAB potentially acting as probiotics capable of reducing cholesterol. The cholesterol-lowering effect especially occurs when the cells are metabolically active.

Angiotensin-I-Converting Enzyme Inhibitory Peptides in Goat Milk Fermented by Lactic Acid Bacteria Isolated from Fermented Food and Breast Milk

Yuliana Tandi Rubak,Lilis Nuraida,Dyah Iswantini,Endang Prangdimurti 한국축산식품학회 2022 한국축산식품학회지 Vol.42 No.1

In this study, angiotensin-I-converting enzyme inhibitory (ACEI) activity was evaluated in fermented goat milk fermented by lactic acid bacteria (LAB) from fermented foods and breast milk. Furthermore, the potential for ACEI peptides was identified in fermented goat milk with the highest ACEI activity. The proteolytic specificity of LAB was also evaluated. The 2% isolate was inoculated into reconstituted goat milk (11%, w/v), then incubated at 37℃ until pH 4.6 was reached. The supernatant produced by centrifugation was analyzed for ACEI activity and total peptide. Viable cell counts of LAB and titratable acidity were also evaluated after fermentation. Peptide identification was carried out using nano liquid chromatography mass spectrometry (LC-MS/MS), and potential as an ACEI peptide was carried out based on a literature review. The result revealed that ACEI activity was produced in all samples (20.44%–60.33%). Fermented goat milk of Lc. lactis ssp. lactis BD17 produced the highest ACEI activity (60.33%; IC50 0.297±0.10 mg/mL) after 48 h incubation, viable cell counts >8 Log CFU/mL, and peptide content of 4.037±0.27/mL. A total of 261 peptides were released, predominantly derived from casein (93%). The proteolytic specificity of Lc. lactis ssp. lactis BD17 through cleavage on the amino acid tyrosine, leucine, glutamic acid, and proline. A total of 21 peptides were identified as ACEI peptides. This study showed that one of the isolates from fermented food, namely Lc. lactis ssp. lactis BD17, has the potential as a starter culture for the production of fermented goat milk which has functional properties as a source of antihypertensive peptides.

The Role of Government Regulations in Enhancing Corporate Social Responsibility Disclosure and Firm Value

FAISAL, Faisal,SITUMORANG, Lilis Suryani,ACHMAD, Tarmizi,PRASTIWI, Andri Korea Distribution Science Association 2020 The Journal of Asian Finance, Economics and Busine Vol.7 No.8

This study investigates, first, whether the extent of corporate social and environmental responsibility disclosure (CSERD) differs between 2010 and 2014; second, whether government regulation affects the extent of CSERD; and, third, whether the CSERD is valued by investors. Content analysis method was used to extract 466 companies' annual reports to measure the extent of social and environmental responsibility disclosure based on the Global Reporting Initiative (GRI) checklist. Independent sample t-test and multivariate regression analysis were also conducted to test the differences of the extent of CSERD as well as determinants and consequence of CSERD. Our results show that the extent of CSERD in 2014 is 21.60 percent higher than in 2010 (13.39 percent). Government regulation has a significant effect on the extent of CSERD. This study also finds that market values positively CSER information disclosed by company. Given that government regulation has a positive impact, however, the findings of this study suggests that the extent of CSERD is still low. To enhance CSERD, government should continuously encourage companies to abide by the regulations as mandated. This study provides a more comprehensive insights of CSRED practices from an emerging country and the effect of government regulation in enhancing CSERD.

Folate in Milk Fermented by Lactic Acid Bacteria from Different Food Sources

Fenny Amilia Mahara,Lilis Nuraida,Hanifah Nuryani Lioe 한국식품영양과학회 2021 Preventive Nutrition and Food Science Vol.26 No.2

Folates are essential micronutrients, and folate deficiency still occurs in many countries. Lactic acid bacteria (LAB) are known to be able to synthesize folates during fermentation, but the folate production is strain-dependent and influenced by the fermentation medium, presence of a folate precursor, and fermentation time. This study aimed to screen extracellular folate-producing LAB from local food sources and evaluate the factors influencing their folate biosynthesis during milk fermentation. The selection of folate-producing LAB was based on their ability to grow in folate-free medium (FACM), with folate concentrations quantified by microbiological assay. Growth of the 18 LAB in FACM varied between isolates, with only 8 isolates growing well and able to synthesize extracellular folate at relatively high concentrations (up to 24.27 ng/mL). The isolates with highest extracellular folate levels, Lactobacillus fermentum JK13 from kefir granules, Lactobacillus plantarum 4C261 from salted mustard, and Lactobacillus rhamnosus R23 from breast milk, were applied to milk fermentation. The last two isolates were probiotic candidates. The three isolates consumed folate when it was present in the milk, and its consumption was in line with their growth. The availability of folate precursors affected the amount of folate consumed, but did not lead to increased folate concentrations in the medium after 72 h fermentation. The results of this study indicate that these isolates cannot be utilized for producing folate in folate-containing milk, as it shows feedback inhibition on folate biosynthesis.

Corporate Governance and Environmental Performance: How They Affect Firm Value

WAHIDAHWATI, Wahidahwati,ARDINI, Lilis Korea Distribution Science Association 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.2

This study aims to examine the effect of environmental performance and good corporate governance (GCG) on the firm values mediated by corporate social responsibility (CSR). The sample in this study was obtained using a purposive sampling method and collected from 205 companies. The analytical method used is moderating regression analysis. The results of this study indicate, first, that corporate social responsibility affects the value of the company. The results of this study indicate that the better corporate governance will increase the value of the firm and vice versa. Second, corporate social responsibility has a direct effect on the firm value, but the effect is still smaller when compared with the internal mechanisms of good corporate governance. This study also found that corporate social responsibility cannot mediate the effect of good corporate governance on firm value. Third, the company's environmental performance influences the company's value. Finally, the effect of environmental performance on company value will be better if mediated by corporate social responsibility. This result shows that environmental performance is a proof that the company's environmental and social concern, which is manifested in corporate social responsibility, will be responded positively by the market so that it will increase share prices (firm value).

Hypothetical Regulation of Folate Biosynthesis and Strategies for Folate Overproduction in Lactic Acid Bacteria

Fenny Amilia Mahara,Lilis Nuraida,Hanifah Nuryani Lioe,Siti Nurjanah 한국식품영양과학회 2023 Preventive Nutrition and Food Science Vol.28 No.4

Folate (vitamin B9) is an essential nutrient for cell metabolism, especially in pregnant women; however, folate deficiency is a major global health issue. To address this issue, folate-rich fermented foods have been used as alternative sources of natural folate. Lactic acid bacteria (LAB), which are commonly involved in food fermentation, can synthesize and excrete folate into the medium, thereby increasing folate levels. However, screening for folate-producing LAB strains is necessary because this ability is highly dependent on the bacterial strain. Some strains of LAB consume folate, and their presence in a fermentation mix can lower the folate levels of the final product. Since microorganisms efficiently regulate folate biosynthesis to meet their growth needs, some strains of folate-producing LAB can deplete folate levels if folate is available in the media. Such folate-efficient producers possess a feedback inhibition mechanism that downregulates folate biosynthesis. Therefore, the application of folate-overproducing strains may be a key strategy for increasing folate levels in media with or without available folate. Many studies have been conducted to screen folate-producing bacteria, but very few have focused on the identification of overproducers. This is probably because of the limited understanding of the regulation of folate biosynthesis in LAB. In this review, we discuss the roles of folate-biosynthetic genes and their contributions to the ability of LAB to synthesize and regulate folate. In addition, we present various hypotheses regarding the regulation of the feedback inhibition mechanism of folate-biosynthetic enzymes and discuss strategies for obtaining folate-overproducing LAB strains.

Performance Analysis of a Combined Blade Savonius Wind Turbines

Arifin Sanusi,Sudjito Soeparman,Slamet Wahyudi,Lilis Yuliati 한국유체기계학회 2017 International journal of fluid machinery and syste Vol.10 No.1

The Savonius wind turbine has a lower performance than other types of wind turbines which may attract more study focus on this turbine. This study aimed to improve wind turbine performance by combining a conventional blade with an elliptical blade into a combined blade rotor. The analysis was performed on three blade models in computational fluid dynamics (CFD) using ANSYS_Fluent Release 14.5. Then the results were verified experimentally using an open wind tunnel system. The results of the numerical simulation were similar to the experimental and showed that the combined blade rotor has better dragging flow and overlap flow than the conventional and elliptical blade. Experimental verification showed that the combined blade was to increase the maximum coefficient of power (Cpmax.) by 11% of the conventional blade and to 5.5% of the elliptical blade.