HMGB1 regulates autophagy through increasing transcriptional activities of JNK and ERK in human myeloid Leukemia cells

( Ming Yi Zhao ),( Ming Hua Yang ),( Liang Chun Yang ),( Yan Yu ),( Min Xie ),( Shan Zhu ),( Rui Kang ),( Dao Lin Tang ),( Zhi Gang Jiang ),( Wu Zhou Yuan ),( Xiu Shan Wu ),( Li Zhi Cao ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.9

HMGB1 is associated with human cancers and is an activator of autophagy which mediates chemotherapy resistance. We here show that the mRNA levels of HMGB1 are high in leukemia cells and it is involved in the progression of childhood chronic myeloid leukemia (CML). HMGB1 decreases the sensitivity of human myeloid leukemia cells K562 to anti-cancer drug induced death through up-regulating the autophagy pathway, which is confirmed by the observation with an increase in fusion of autophagosomes and autophagolysosomes. When overexpressing HMGB1, both mRNA levels of Beclin-1, VSP34 and UVRAG which are key genes involved in mammalian autophagy and protein levels of p-Bcl-2 and LC3-II are increased. Luciferase assays document that over-expression of HMGB1 increases the transcriptional activity of JNK and ERK, which may be silenced by siRNA. The results suggest that HMGB1 regulates JNK and ERK required for autophagy, which provides a potential drug target for therapeutic interventions in childhood CML. [BMB reports 2011; 44(9): 601-606]

Characterization of composite prepared with different mixing ratios of TiO₂ to activated carbon and their photocatalytic activity

Ming-Liang Chen,Jang-Soon Bae,Young-Shin Ko,Won-Chun Oh 한국분석과학회 2006 분석과학 Vol.19 No.5

Mitochondrial DNA variation and phylogeography of Old World camels

Ming Liang,Siren Dalai,Yi Li,Hai Le,He Jing,Ji Rimutu 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.4

Objective: Old World camels are a valuable genetic resource for many countries around the world due to their adaptation to the desert environment. At present, Old World camels have encountered the challenge of unprecedented loss of genetic resources. Through our research, we would reveal the population structure and genetic variation in Old World camel populations, which provides a theoretical basis for understanding the germplasm resources and origin and evolution of different Old World camel populations. Methods: In the present study, we assessed mtDNA control region sequences of 182 individuals from Old World camels to unravel genetic diversity, phylogeography, and demographic dynamics. Results: Thirty-two haplotypes confirmed by 54 polymorphic sites were identified in the 156 sequences, which included 129 domestic and 27 wild Bactrian camels. Meanwhile, 14 haplotypes were defined by 47 polymorphic sites from 26 sequences in the dromedaries. The wild Bactrian camel population showed the lowest haplotype and nucleotide diversity, while the dromedaries investigated had the highest. The phylogenetic analysis suggests that there are several shared haplotypes in different Bactrian camel populations, and that there has been genetic introgression between domestic Bactrian camels and dromedaries. In addition, positive values of Tajima’s D and Fu’s Fs test demonstrated a decrease in population size and/or balancing selection in the wild Bactrian camel population. In contrast, the negative values of Tajima’s D and Fu’s Fs test in East Asian Bactrian camel populations explained the demographic expansion and/or positive selection. Conclusion: In summary, we report novel information regarding the genetic diversity, population structure and demographic dynamics of Old World camels. The findings obtained from the present study reveal that abundant genetic diversity occurs in domestic Bactrian camel populations and dromedaries, while there are low levels of haplotype and nucleotide diversity in the wild Bactrian camel population. Objective: Old World camels are a valuable genetic resource for many countries around the world due to their adaptation to the desert environment. At present, Old World camels have encountered the challenge of unprecedented loss of genetic resources. Through our research, we would reveal the population structure and genetic variation in Old World camel populations, which provides a theoretical basis for understanding the germplasm resources and origin and evolution of different Old World camel populations.Methods: In the present study, we assessed mtDNA control region sequences of 182 individuals from Old World camels to unravel genetic diversity, phylogeography, and demographic dynamics.Results: Thirty-two haplotypes confirmed by 54 polymorphic sites were identified in the 156 sequences, which included 129 domestic and 27 wild Bactrian camels. Meanwhile, 14 haplotypes were defined by 47 polymorphic sites from 26 sequences in the dromedaries. The wild Bactrian camel population showed the lowest haplotype and nucleotide diversity, while the dromedaries investigated had the highest. The phylogenetic analysis suggests that there are several shared haplotypes in different Bactrian camel populations, and that there has been genetic introgression between domestic Bactrian camels and dromedaries. In addition, positive values of Tajima’s D and Fu’s <i>Fs</i> test demonstrated a decrease in population size and/or balancing selection in the wild Bactrian camel population. In contrast, the negative values of Tajima’s D and Fu’s <i>Fs</i> test in East Asian Bactrian camel populations explained the demographic expansion and/or positive selection.Conclusion: In summary, we report novel information regarding the genetic diversity, population structure and demographic dynamics of Old World camels. The findings obtained from the present study reveal that abundant genetic diversity occurs in domestic Bactrian camel populations and dromedaries, while there are low levels of haplotype and nucleotide diversity in the wild Bactrian camel population.

Photocatalytic Effect for TiO<SUB>2</SUB>/ACF Composite Electrochemically Prepared with TNB Electrolyte

Ming-Liang Chen,Chang-Sung Lim,Won-Chun Oh 한국탄소학회 2007 Carbon Letters Vol.8 No.3

[ TiO2 ]ACF composites were prepared by the electrochemical method with Titanium (IV) n-butoxide (TNB) electrolyte under different electrochemical operation time. The BET surface area for TiO2/ACF composites decrease with the increase of electrochemical operation time. There is a single crystal structure which is anatase in all of the samples from the data of XRD. The SEM micrphotographs of TiO2/ACF composites show that the TiO2 particles were well mixed with the ACF. There are O and P with strong C and Ti peaks in all samples from EDX results, and it also shows that a decrease of the C content with a increasing of Ti content with increasing of the electrochemical operation time in the over all composites. DSC cures show that the exothermic peak of all composites at 560℃ represents the transformation heat of amorphous parts to anatase phase and the discontinuous grain growth of the transformed anatase particles. Finally, the excellent photoactivity of TiO2/ACF composites (especially, ACFT10) could be attributed that the decrease of concentration of MB can be concluded to be much faster for the adsorption by ACF than for photocatalytic decomposition by TiO2.

In Vitro Selection For Large Patch Resistance In Zoysiagrass

Ming-Liang Chai,Jong-Min Lee(이종민),Doo-Hwan Kim(김두환) 한국원예학회 2000 원예과학기술지 Vol.18 No.5

Photocatalytic Degradation of Methylene Blue by CNT/TiO2 Composites Prepared from MWCNT and Titanium n-butoxide with Benzene

Ming-Liang Chen,Feng-Jun Zhangl,오원춘 한국세라믹학회 2008 한국세라믹학회지 Vol.45 No.11

In this study, CNT/TiO2 composites were prepared using surface modified Multiwall carbon nanotube (MWCNT) and titanium n-butoxide (TNB) with benzene. The composites were characterized by nitrogen adsorption isotherms, scanning electron microscopy (SEM), X-ray diffraction (XRD), energy dispersive X-ray analysis (EDX), FT-IR spectra, and UV-vis absorption spectroscopy. The UV radiation induced photoactivity of the CNT/TiO2 composites was tested using a fixed concentration of methylene blue (MB, C16H18N3S· Cl·3H2O) in an aqueous solution. Finally, it can be considered that the MB removal effect of the CNT/TiO2 composites is not only due to the adsorption effect of MWCNT and photocatalytic degradation of TiO2, but also to electron transfer between MWCNT and TiO2. In this study, CNT/TiO2 composites were prepared using surface modified Multiwall carbon nanotube (MWCNT) and titanium n-butoxide (TNB) with benzene. The composites were characterized by nitrogen adsorption isotherms, scanning electron microscopy (SEM), X-ray diffraction (XRD), energy dispersive X-ray analysis (EDX), FT-IR spectra, and UV-vis absorption spectroscopy. The UV radiation induced photoactivity of the CNT/TiO2 composites was tested using a fixed concentration of methylene blue (MB, C16H18N3S· Cl·3H2O) in an aqueous solution. Finally, it can be considered that the MB removal effect of the CNT/TiO2 composites is not only due to the adsorption effect of MWCNT and photocatalytic degradation of TiO2, but also to electron transfer between MWCNT and TiO2.

The Composition and Characteristics of the Coastal Space of Chongqing: An Interpretative Study Based on the Ten-year Draft Construction Plan of the Alternate Capital

( Ming-liang Kong ),( Chun-lan Du ),( Hua-song Mao ),( Jun-hua Zhang ),( Kwang-min Ham ),( Jo Hyunju ) 한국환경과학회 2016 한국환경과학회지 Vol.25 No.7

This study focused on the buildings, wharves, and parks of the coastal space of Chongqing based on the ten-year construction draft plan of the alternate capital. From an investigation of these three components according to their type, structure, terrain, function, spatial layer, and so on, the following conclusions were drawn: The coastal space of Chongqing is composed of three different layers. The first and second layers consisted of the two-river intersection basin with the transportation and commemorative space at the center, the Yangtze River basin with the commercial space at the center, and the Jialing River with the military and recreational space at the center. The third-layer is centered around the commemorative space. The coastal space of Chongqing manifested the limitations of the times, its traditionality, and an integrated development process.

Preparation of AC/TiO<SUB>2</SUB> Composites from Activated Carbon Modified by HNO<SUB>3</SUB> and Their Photocatalytic Activity

Ming-Liang Chen,Won-Chun Oh 한국탄소학회 2007 Carbon Letters Vol.8 No.2

In this work, activated carbon (AC) after HNO3 modification was used as the support during the production of supported TiO2 to increase the high deposition efficiency and the photocatalytic activity. The results of N2 adsorption showed that the BET surface area of samples decreased with an increasing of the concentration of HNO3 due to the penetration of TiO2. From XRD data, a single crystal structure of anatase peak was observed in diffraction patterns for the AC coated with titanium complexes. From the SEM results, almost all particles were aggregated with each other at the carbon surface and AC was covered with TiO2 particles in all of the samples. The EDX spectra show the presence of C, O, Ti and other elements. It was also observed a decreasing of amount of C content with increasing Ti and O content from the EDX. The results of FT-IR revealed that the modified AC contained more surface oxygen bearing groups than that of the original AC. The effect of surface acidity and basity calculated from Boehm titration method was also evaluated from correlations as a function of NaOH, NaHCO3, and Na2CO3 uptake. The surface modification of AC by HNO3 leads to an increase in the catalytic efficiency of AC/TiO2 catalysts, and the catalytic efficiency increases with increasing of HNO3 concentration.

Carbon/TiO<SUB>2</SUB> Prepared from Anatase to Pitch and their Photocatalytic Performance

Ming-Liang Chen,Young-Shin Ko,Won-Chun Oh 한국탄소학회 2007 Carbon Letters Vol.8 No.1

Carbon/TiO2 composites were prepared by CCl4 solvent mixing method with different mixing ratios. Since the carbon layers derived from pitch on the TiO2 particles were porous, the Carbon/TiO2 composite series showed a good adsorptivity and photo decomposition activity. The BET surface area for the carbon layer in the sample increases to increasing with pitch contents. The SEM results present to the characterization of porous texture on the Carbon/TiO2 composite and pitch distributions on the surfaces for all the materials used. From XRD data, a weak and broad carbon peak of graphene with pristine anatase peaks were observed in the X-ray diffraction patterns for the Carbon/TiO2. The EDX spectra show the presence of C, O and S with strong Ti peaks. Most of these samples are richer in carbon and major Ti metal than any other elements. Finally, the excellent photocatalytic activity of Carbon/TiO2 with slope relationship between relative concentration (C/C0) of MB and t could be attributed to the homogeneous coated pitch on the external surface by CCl4 solvent method.

Stratigraphic Sequence and Deposition-affected Compressibility of Fine-grained Sediments in the Ancient Yellow River Delta during the Late Pleistocene and Holocene

Ming-Zhi Zhao,Qiang Luo,Liang-Wei Jiang,Ming Wei 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.1

To investigate the stratigraphic sequence in the ancient Yellow River Delta and further discuss the correlation of compressibility of undisturbed soils with depositional environments, two boreholes were drilled at Liyao test site in Cangzhou, Hebei Province, China. Some basic tests regarding to index properties were performed on sediments from one borehole, and oedometer test was conducted for the “undisturbed samples” collected from the other. The stratigraphic sequence in the study area is classified into four depositional systems in terms of sedimentation environment. Evaluation of sample disturbance shows that the “undisturbed samples” for oedometer test are just slightly disturbed in the sampling process. Since overall compressibility can be divided into intrinsic compressibility and additional one, the environmental factors that can affect soil microstructure are closely correlated with additional compressibility. Thus, the influencing mechanism of depositional rate on additional void index ΔIv was investigated quantitatively. Moreover, the relationship between floating range of yield stress and buried depth is established. Then, the additional compressibility of undisturbed samples can be evaluated with depositional rate and buried depth. Finally, the predicted overall compression curve with the proposed method in this study is compared with the measured curve to verify the reasonability and feasibility.