Penicillium sp. ZD-Z1 Chitosanase Immobilized on DEAE Cellulose by Cross-linking Reaction

Lian-Ying Zheng,Yu-Liang Xiao 한국화학공학회 2004 Korean Journal of Chemical Engineering Vol.21 No.1

obtained from Penicillium sp.ZD-Z1 was immobilized on DEAE celulose with glutaralde-hyde by cross-linking reaction. The optimal conditions of immobilization were as follows: 0.1 g DEAE cellulose wastreated with 5 ml 5% glutaraldehyde solution; then 2.3 mg chitosanase was immobilized on the carrier. The optimaltemperature and pH was 60oC and 4.0, and the Km value was 18.87 g/L. Under optimal conditions, the activity of im-mobilized enzyme is 1.5 U/g, and the recovery of enzyme activity is 81.3%. After immobilization, the optimal tem-perature and Km value increased (from 50oC to 60oC, from 2.49 g/L to 18.87 g/L), whereas the optimal pH was re-duced (from 5.0 to 4.0). The enzyme activity los was les than 20% after 10 times batch reaction; the imobilizedenzyme showed good operation stability.

Control synthesis of magnetic Fe3O4–chitosan nanoparticles under UV irradiation in aqueous system

Lian-ying Zhang,Xin-jun Zhu,Han-wen Sun,Gui-rong Chi,Jun-xiao Xu,Yong-ling Sun 한국물리학회 2010 Current Applied Physics Vol.10 No.3

Novel magnetic Fe3O4–chitosan nanoparticles were synthesized via photochemical method in an emulsifier-free aqueous system at room temperature for the first time. The scanning electron microscopy (SEM)and transmission electron microscopy (TEM) results showed that the Fe3O4–chitosan nanoparticles were in regular shape with a mean diameter of 41 nm, whereas the average size in aqueous solution measured by photocorrelation spectroscopy (PCS) was 64 nm, which indicated that the nanoparticles had waterswelling properties. X-ray diffraction (XRD) patterns indicated that the Fe3O4 nanoparticles were pure Fe3O4 with a spinel structure, and the irradiation under UV light did not result in a phase change. The Fe3O4–chitosan nanoparticles were also characterized by Fourier transform infrared (FTIR) spectra, thermogravimetric analysis (TGA) and vibrating sample magnetometer (VSM). Magnetic measurement revealed that the saturated magnetization (Ms) of the Fe3O4–chitosan nanoparticles reached 48.6 emu/g and the nanoparticles showed the characteristics of superparamagnetism. The stability test showed these novel nanoparticles had high magnetic stability. The PCS and TGA results indicated that the size and chitosan content of Fe3O4–chitosan nanoparticles formed was pH- and chitosan/Fe3O4 ratio-dependent,which could be used to synthesize magnetic Fe3O4–chitosan nanoparticles with different size to meet the requirements of different applications.

The Impact of Risk Perception on Developing Incentive Systems for Relational Contracting

Lian-Ying Zhang,Fei Li 대한토목학회 2015 KSCE JOURNAL OF CIVIL ENGINEERING Vol.19 No.5

Financial incentives, specifically refer to risk/reward sharing arrangements, have been seen as an important way of facilitating relational contracting. Although there has been a great interest in developing appropriate incentive systems, still very little literature focuses on the impact of risk perception on risk/reward sharing arrangements. Taking into account risk characteristics in relational contracting, this study proposes a theoretical model in order to extend the understanding of relational and performance risk perception to the formulation of financial incentives from social and individual perspectives, and then validates the model for assessing the relationships among social and individual factors, risk perception and financial incentives with structural equation modeling in the context of integrated project teams. Data collected from 238 members in China can provide support for the proposed model. The results demonstrate that both relational and performance risk perception are significantly related to financial incentive, and social interaction ties, trust, shared vision, control ability and self-efficacy can reduce participants’ perceived level of risk. This study suggests that participants’ social capital and individual cognition should not be ignored when developing incentive systems for relational contracting.

Penicillium sp. ZD-Z1 Chitosanase Immobilized on DEAE Cellulose by Cross-linking Reaction

Zheng, Lian-Ying,Xiao, Yu-Liang 한국화학공학회 2004 Korean Journal of Chemical Engineering Vol.21 No.1

Chitosanase obtained from Penicillium sp.ZD-Z1 was immobilized on DEAE cellulose with glutaraldehyde by cross-linking reaction. The optimal conditions of immobilization were as follows: 0.1g DEAE cellulose was treated with 5㎖ 5% glutaraldehyde solution; then 2.3㎎ chitosanase was immobilized on the carrier. The optimal temperature and pH was 60℃ and 4.0, and the K_(m) value was 18.87g/L. Under optimal conditions, the activity of immobilized enzyme is 1.5U/g, and the recovery of enzyme activity is 81.3%. After immobilization, the optimal temperature and K_(m) value increased (from 50℃ to 60℃, from 2.49g/L to 18.87g/L), whereas the optimal pH was reduced (from 5.0 to 4.0). The enzyme activity loss was less than 20% after 10 times batch reaction; the immobilized enzyme showed good operation stability.

Effects of Preemptive Analgesia with Parecoxib Sodium on Haemodynamics and Plasma Stress Hormones in Surgical Patients with Thyroid Carcinoma

Wang, Lian-Dong,Gao, Xia,Li, Jun-Ying,Yu, Hong-Yan,Su, Hai-Wen,Liu, Lian-Zhong,Qi, Jun Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9

Background: To investigate the effects of preemptive analgesia with parecoxib sodium on haemodynamics and plasma stress hormones in surgical patients with thyroid carcinoma. Materials and Methods: Fifty-seven patients with thyroid carcinoma who underwent thyroidectomy selectively in Laiwu Hospital Affiliated to Taishan Medical University and Binzhou People's Hospital were selected and randomly divided into three groups, 19 cases in each group. The control group was intravenously injected 0.9% sodium chloride injection before anesthesia induction; trial group I was intravenously injected with parecoxib sodium 20 min before anesthesia induction; based on trial group I, trial group II was injected with parecoxib sodium again 12 h after surgery. The levels of plasma norepinephrine (NE), cortisol (Cor) and blood glucose before, 12 and 24 h after surgery and changes of haemodynamics before surgery, at the end of surgery and 12, 24 and 48 h after surgery were compared in the three groups. Besides, visual analogue scale (VAS) scores were recorded 4, 8, 12 and 24 h after surgery. Results: 12 and 24 h after surgery, the levels of plasma NE and Cor in three groups rose dramatically (P<0.05 or (P<0.01); The levels of plasma NE and Cor in trial groups I and II were evidently lower than in control group (P<0.05 or P<0.01), and those in trial group II slightly lower than in trial group I. 12 h after surgery, the heart rates (HR) and systolic pressures (SBP) in trial groups I and II increased obviously by comparison to surgery before (P<0.05 or P<0.01), but gradually returned to the preoperative level. HR, SBP and diastolic pressures (DBP) in trial groups I and II at the end of surgery and 12 h after surgery were all lower than in the control group, and significant differences were present (P<0.05 or (P<0.01). At 4, 8, 12 and 24 h after surgery, VAS scores in trial groups I and II were markedly lower than in the control group (P<0.05 or (P<0.01), the scores in trial group II being the lowest. Conclusions: Combined application of parecoxib sodium for preemptive analgesia before anesthesia and after surgery can effectively reduce the levels of plasma stress hormones and improve analgesic effects in surgical patients with thyroid carcinoma, and without conspicuous impact on haemodynamics.

온라인 협업 도구 활용 한국어 수업 사례 : 상해 삼달대학교를 중심으로

배영련(Pei, Ying Lian),오선자(Wu, Shan Zi),최선진(Choi, Seon Jin),한나(Han, Na),오영화(Wu, Ying Hua) 글로벌교육연구학회 2021 글로벌교육연구 Vol.13 No.4

본 연구는 온라인 수업에서의 상호작용에 도움을 주며, 중국과 한국에서 동시에 사용할수 있는 다양한 온라인 협업 도구를 소개하고, 이 도구 네 가지(mentimeter, zoom, padlet, Quizizz)를 사용하여 중국인 대상 한국어 수업을 운영한 사례를 제시하였다. 그리고본 수업 운영에 대한 학습자 만족도를 살펴보기 위하여 2021학년도 3월 학기에 본 수업을수강한 상해 삼달대학교 한국어 학습자 75명을 대상으로 만족도 조사를 시행하였으며, 그결과 전체적인 수업 만족도는 평균 4.09였다. 또한, 온라인 수업에서의 상호작용 향상을위한 방법으로 활용한 온라인 협업 도구에 대한 전체적인 만족도는 4.24였으며, 각 도구의만족도는 mentimeter가 4.63, zoom이 4.15, padlet이 4.09, Quizizz 3.81 순이었다. 이 중에서 협업 도구에 대한 전체적인 만족도와 padlet 만족도가 수업 전체 만족도에 영향을미치는 것으로 분석되었다. 본 연구 결과에 따른 제언은 다음과 같다. 첫째, 온라인 협업도구 활용에 대한 학습자 만족도가 높기 때문에 한국어 수업에 활용할 수 있는 다양한 협업도구 연구를 바탕으로 수업 적용이 필요하며, 각 국 학습자용 교재 편찬이 필요하다고 할수 있다. 본 연구는 다양한 온라인 수업에서의 상호작용에 필요한 다양한 협업 도구를 소개하고 비대면 수업에서의 실제 활용방안을 제시했으며, 이에 대한 학습자들의 의견을 수렴했다는 점에서 의의가 있다. This study was to present a case of an Online Korean class using four online tools such as Mentimeter, Zoom, Padlet, Quizizz for helping the interaction of Korean and Chinese students. To investigate learner satisfaction, 75 Chinese students from Shanda University participated in a survey after the class. The results were as follows: First, the overall class satisfaction was 4.09, especially students were satisfied with using collaboration tools. Second, online tool satisfaction was 4.63 for Mentimeter, 4.15 for Zoom, 4.09 for Padlet, and 3.81 for Quizizz in the order of the program. Based on the results, it was suggested applications of various online tools into class and textbooks for learners. At last, it was significant that the case of online Korean class was presented with many online tools and learners satisfaction in this study.

对韩国留学生的“把”字句教学方法探析

김영실(Jin Ying Shi)(金英实) , 이길연(Li Ji Lian)(李吉莲) 대한중국학회 2016 중국학 Vol.57 No.-

In the teaching of Chinese as a foreign language, Ba-construction is a difficult grammar items. In the study of Chinese ontology, there are quite a few articles on Ba-construction, and the study of Ba-construction in Chinese as a foreign language teaching has also achieved remarkable results. In recent years, more and more attention has been paid to the teaching of Ba-construction and based on contrastive analysis or language acquisition research. The study of Ba-construction is gradually developed from the background of the cross-linguistic to the background of specific native language. The students mother tongue has a great influence on Ba-construction learning. Korean is an Altaic language and Chinese is a Sino-Tibetan language. There is a big difference between the two languages. The deep semantic relations of Chinese language are expressed by superficial syntactic relations of Korean language, and The differences between different semantic types and semantic orientations directly affect its correspondence in Korean language. The Ba-construction with different Semantic type have neat corresponding forms in Korean. However, Korean students try to find the similarity between the sentence pattern and native language in the process of Ba-construction acquisition, so make a lot of error sentences. This paper tries to classify Ba-construction into four categories from the perspective of semantic type and the corresponding characteristics of Korean, and discusses the teaching methods of Ba-construction for korean students. 在对外汉语教学中, “把”字句是一个习得难度较高的语法项目。语言类型即学生的母语背景对“把”字句的习得有显著影响。近年来对“把”字句教学越来越重视基于对比分析和习得研究, “把”字句研究也从泛母语背景者逐渐发展为特定母语背景者在特定学习阶段的习得考察。韩国语属阿尔泰语系, 是黏着语;而汉语属汉藏语系, 是孤立语, 两者差别较大。韩国语把汉语的深层语义关系用表层句法关系来表达, 不同语义类型及语义指向的差异直接影响它在韩国语中的对应关系。不同语义类型的“把”后宾语在韩国语中存在比较整齐的对应形式。但韩国留学生在“把”字句习得过程中, 努力寻求所学句型与母语相似的模式, 不恰当地类比推理, 形成“把”字句与母语中宾格形式等同的假设, 导致偏误句的出现。本文试从“把”后宾语NP的语义类型及其在韩国语的对应形式将“把”字句分为四类, 并结合各类“把”字句的谓语语义指向及其在韩国语中的表达方式探讨各类“把”字句的教学方法。

Comparison of Two Cases of Strong Increase in the Bottom Temperature in the Yellow Sea in Winter

Lian-Gang Lü,Guang-Bing Yang,Zong-Wei Liu,Ying Jiang,Chunmei Yang,Chao Zhang 한국해양과학기술원 2015 Ocean science journal Vol.50 No.3

Mooring observations were carried out on the western slope of the southern Yellow Sea (YS) to observe the Yellow Sea Warm Current (YSWC). Strong increases in the bottom temperature (about 3°C within 1 day) were observed at mooring M5 (shelf break) on 8 January 2007, and at mooring A2 (mid-slope) on 5 December 2008. The strong temperature increase of bottom water at M5 was closely related to the burst of the YSWC. The bottom temperature at mooring A2 increased and decreased alternately from 7 November to 14 December 2008, and the strong increase (about 3°C within 1 day) occurring on 5 December was one of the four rises during that period. The significant semi-diurnal-oscillation during that period indicated that the thermal fronts outlining the Yellow Sea Cold Water Mass (YSCWM) boundary was very close to location A2. The notable rises in the bottom temperature at A2 were associated with bottom eastward currents, while the distinct falls in temperature coincide with bottom westward currents. The distinctive distribution in the bottom temperature associated with the YSCWM and the bottom eastward currents were mainly responsible for the strong increase in the bottom temperature at A2. The variations in the bottom temperature described here are valuable for understanding the time evolution of the YSWC in winter and the YSCWM from late autumn to early winter.

Study on the interaction of compound bonded magnets

Ying Chang,Fazeng Lian,Xiaojun Yu 한국자기학회 2003 한국자기학회 학술연구발표회 논문개요집 Vol.13 No.2

Study on the Interaction of Compound Bonded Magnets

Ying Chang,Nuo Ma,Xiaojun Yu,Fazeng Lian,Wei Li 한국자기학회 2004 Journal of Magnetics Vol.9 No.4

The attempt for the addition of double-phase nanocomposite Nd₂Fe₁₄B/Fe₃B powders, respectively, into several RE₂Fe₁₄B (RE=Pr, Nd) powders with high magnetic properties was carried out. The powders were compounded and compressed to take shape bonded magnets. By means of investigating the variation of compound magnet Br, the interaction between magnetic powders was revealed. The result shows that not chemical just but physical interaction exists between elements. The compound effect of Nd₂Fe₁₄B/Fe₃B-ferrite bonded magnets was detailed studied. The functional relation was revealed between magnetic properties and ferrite content. That is Y = 5.42 x²-11.34 x + 6.62. The variation of iHc temperature coefficient β_(iHc) with ferrite content was investigated. Following the ferrite content increased, β_(iHc) and hirr were obviously decreased, compression-resistant strength was enhanced.