Effect of additives and filling methods on whole plant corn silage quality, fermentation characteristics and in situ digestibility

Jiao, Ting,Lei, Zhaomin,Wu, Jianping,Li, Fei,Casper, David P.,Wang, Jianfu,Jiao, Jianxin Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.11

Objective: This project aimed to evaluate the effects of both different additives and filling methods on nutritive quality, fermentation profile, and in situ digestibility of whole plant corn silage. Methods: Whole plant corn forage harvested at 26.72% dry matter (DM) was chopped and treated with two filling methods, i) fill silos at one time (F1), ii) fill silos at three times (F3), packing samples into one/three silo capacity at the first day, another one/three capacity at the second day, then one/three at the third day, three replicates. For each replicate, samples were treated with three additives, i) control (CTRL, no additive), ii) Sila-Max (MAX, Ralco Nutrition Inc., Marshall, MN, USA), and iii) Sila-Mix (MIX, Ralco Nutrition Inc., USA). With three replicates of each secondary treatment, there were nine silos, 54 silos in total. Each silo had a packing density of 137.61 kg of DM/m3. All silos were weighed and stored in lab at ambient temperature. Results: After 60 d of ensiling, all items showed good silage fermentation under MAX filled one time or three times (p<0.01). Higher silage quality for all additives was obtained at filling one time than that filled three times (p<0.01). The highest DM and lowest DM loss rate (DMLR) occurred to MAX treatment at two filling methods (p<0.01); Digestibility of acid detergent fiber, neutral detergent fiber (NDF), and curde protein had the same results as silage quality (p<0.01). Yield of digestible DM and digestible NDF also showed higher value under MAX especially for filling one time (p<0.05). Conclusion: All corn silages showed good fermentation attributes (pH<4.0). The forage filled one time had higher silage quality than that filled three times (p<0.01). MAX with homofermentative lactic acid bacteria enhanced the lactic acid fermentation, silage quality and nutrient digestibility, and so improved the digestible nutrient yield.

Complete Genome Sequence of Salmonella enterica Serovar Pullorum Multidrug Resistance Strain S06004 from China(s)

( Qiu Chun Li ),( Ya Chen Hu ),( Yin Fei Wu ),( Xiao Chun Wang ),( Xiao Lei Xie ),( Ming Xin Tao ),( Jun Lei Yin ),( Zhi Jie Lin ),( Yang Jiao ),( Li Juan Xu ),( Xinan Jiao ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.5

As Salmonella enterica serovar Pullorum remains a major economic problem for the poultry industries of countries with no efficient control measures, we presented a multidrug resistance strain S06004 (isolated from a clinically sick chicken in China in 2006) for genome sequencing. The genome comparison showed that the strain contained two prophages, the ST104 and prophage-4 (Fels2) of E. coli LF82, which were not detected in the only published genomes of S. Pullorum RKS5078 and CDC1983-67. In addition, the GyrA Ser83 point mutation, drugresistant genes, and many antibiotic pump systems that are present in S06004 may be contributing to the multidrug resistance of this strain.

Clustering with a Novel Global Harmony Search Algorithm for Image Segmentation

Lei Wang,Yufeng YI,Jiao Liu 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.2

Synthesis and gas sensing properties of high porosity n-type nickel ferrite thin film assisted by altering magnetic field

Lei Zhang,Wanli Jiao 한국물리학회 2015 Current Applied Physics Vol.15 No.7

NiFe2O4 thin film with high porosity based gas sensors had been prepared and their microstructure and gas sensing property were investigated. The sensing layer, consisted of perpendicular overlapped NiFe2O4 chains which were induced by altering magnetic field to self-assemble, had high porosity. The phase character and porous microstructure were characterized by X-ray diffraction (XRD) and a polarizing optical microscopy. The gas sensing tests results indicated that the sensor presented a high sensitivity to NH3 at 150 ℃, and was selective to NH3 below 200 ℃. The large porosity microstructure should benefit the reaction between target gas and sensing material and the detection of low concentration gas at low working temperature. In repeatability tests, the response and recovery time values had only narrow fluctuations.

Effects of Dietary Crude Protein on Growth Performance, Nutrient Utilization, Immunity Index and Protease Activity in Weaner to 2 Month-old New Zealand Rabbits

Lei, Q.X.,Li, F.C.,Jiao, H.C. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.10

An experiment was conducted to determine the effects of different dietary crude protein (CP) levels on growth performance, nutrient utilization, small intestine protease activity and immunity index of weaner to 2 month-old New Zealand rabbits. Eighty weaner rabbits were allocated in individual cages to five treatments in which they were fed diets with CP at 14%, 16%, 18%, 20% and 22%, respectively. The growth performance and nutrient digestibility of rabbits increased firstly when dietary CP increased, then decreased. The average daily gain was the highest and feed conversion rate was the lowest when dietary CP reached 20%, namely 34.9 g/d and 2.74:1, respectively. Maximum CP digestibility was 72.1% in the 18% CP group, maximum crude fiber digestibility of 28.4% occurred in the 16% CP group and was significantly different from other treatments (p<0.01), apparent digestibility of Lys and Val followed the same trend as CP digestibility, and reached their maximum when dietary CP was 18%. Apparent digestibility of Cys, Tyr, Leu and Thr also had a similar trend to CP digestibility. Nitrogen retention (RN) increased with CP level (p>0.05), and was highest for 20% CP treatment (1.5 g/d). The effect of CP level on the rate of digestible nitrogen (DN) converted RN was small. The spleen index, thymus index, chymotrypsin and trypsin activities in small intestine were highest when dietary CP was 16%, which were 1.0, 2.8, 15.7 U/g and 125.7 U/g, respectively. There was no significant difference among treatments (p>0.05). According to the above results, the appropriate dietary CP level from weaner to 2 month-old meat rabbits was 18-20%.

Efficient Mucosal Immunization by Mucoadhesive and pH-Sensitive Polymeric Vaccine Delivery System

Lei Xing,Tian-Jiao Zhou,Ya-Tong Fan,Yu-jing He,Tao Pang,조기현,Jinjian Lu,Hu-Lin Jiang,조종수 한국고분자학회 2019 Macromolecular Research Vol.27 No.3

Mucosal surfaces as the largest immune organ of human body cover 400 m2 of the body including the gastrointestinal, urogenital, and respiratory tracts. The local mucosal immunity is an important first line of defense against many pathogens because most pathogens initiate their infection through access to the mucosal region of body. Also, the mucosal vaccines induce mucosal and systemic immunity simultaneously. Therefore, attracted by the advantages of mucosal immunity, researchers keep an eye on the mucoadhesive and pH-sensitive polymeric vaccine delivery system to solve several limitations of mucosal administration, making mucosal immunity receive great interests lately. In this review, we discuss natural polymer- and synthetic polymer-based mucoadhesive and pHsensitive systems used for mucosal vaccine delivery. Also, we cover how to target antigen presenting cells and M cells for the cell specificity. Finally, we conclude the significant progress in mucosal vaccine and the prospect mucosal vaccine research in the future.

The microwave magnetism of epitaxy LiFe5O8 thin film modulated by thickness

Jiao Yang,Jianfei Lei,Kai Du,Xudong Zheng,Xiujuan Jin 한국물리학회 2020 Current Applied Physics Vol.20 No.4

Spinel lithium ferrite LiFe5O8 (LFO) has attracted robust research interests due to their potential applications in isolators, circulators, and phase shifters. In this work, a series of LFO thin film with various thickness were fabricated on SrTiO3 (STO) single-crystalline substrates by pulsed laser deposition technology. We systematically investigated the influences of the thickness of LFO thin film on the crystal structure and magnetic properties. The in-plane lattice parameter a and in-plane lattice parameter c were modulated by controlling the thickness of LFO thin film, which was confirmed using reciprocal space mappings (RSMs) technology. Furthermore, the microwave magnetism of LFO thin film with various thickness were studied systematically by ferromagnetic resonance (FMR) measurement. Moreover, with increasing the thickness of LFO thin film from 50 to 180 nm, the difference of the ferromagnetic resonance field between easy- and hard-magnetization axis can be enhanced and reach to 330 Oe. These results illustrate that dynamic magnetic properties can be controlled by tuning the thickness of LFO thin film. Our work provides an effective method to tailor the lattice parameter and modify the magnetic properties of the LFO thin film and contributes to further design high-frequency functional device.

Preparation and electrochemical performance of cellular structure Ni(OH)2 thin film

Wanli Jiao,Lei Zhang 한국물리학회 2016 Current Applied Physics Vol.16 No.2

Cellular structure Nickel Hydroxide Ni(OH)2 thin film grown on indium-tin oxide (ITO) substrate was synthesized by a two-step hydrothermal method. The crystal structure and morphology of Ni(OH)2 thin film was investigated by means of X-ray diffraction (XRD) and scanning electron microscopy (SEM), respectively. The electrochemical performance was investigated by a cyclic voltammetry (CV) method. The results indicated that the thin film consisted of plate-like b-Ni(OH)2 grains growing on the substrate vertically and connecting each other to form a kind of multilayer cellular structure, which formed a large number of stable three-dimension pores and facilitated electrolyte diffusion and penetration to the inside of thin film. Thus, the specific capacitance of Ni(OH)2 thin film capacitor was 980 F/g at a low scan rate of 10 mV/s; and the better reversibility and stability indicated that the cellular structure Ni(OH)2 thin film should be a kind of promising capacitor electrode material.

Experimental and Numerical Study on Complex Multi-planar Welded Tubular Joints in Umbrella-Type Space Trusses with Long Overhangs

Jinfeng Jiao,Xiao Ma,Honggang Lei,Y. Frank Chen 한국강구조학회 2018 International Journal of Steel Structures Vol.18 No.5

A test rig with multi-functional purposes was specifi cally designed and manufactured to study the behavior of multi-planar welded tubular joints subjected to multi-planar concurrent axial loading. An experimental investigation was conducted on full-scale welded tubular joints with each consisting of one chord and eight braces under monotonic loading conditions. Two pairs or four representative specimens (two specimens for each joint type) were tested, in which each pair was reinforced with two kinds of diff erent internal stiff eners at the intersections between the chords using welded rectangular hollow steel sections (RHSSs) and the braces using rolled circular hollow steel sections (CHSSs) and welded RHSSs. The eff ects of different internal stiff eners at the chord–brace intersection on the load capacity of joints under concurrent multi-planar axial compression/tension are discussed. The test results of joint strengths, failure modes, and load–stress curves are presented. Finite element analyses were performed to verify the experimental results. The study results show that the two diff erent joint types with the internal stiff eners at the chord–brace intersection under axial compression/tension signifi cantly increase the corresponding ultimate strength to far exceed the usual design strength. The load carrying capacity of welded tubular joints decreases with a higher degree of the manufacturing imperfection in individual braces at the tubular joints. Furthermore, the interaction eff ect of the concurrent axial loading applied at the welded tubular joint on member stress is apparent.

Association between the TP53BP1 rs2602141 A/C Polymorphism and Cancer Risk: A Systematic Review and Meta-Analysis

Liu, Lei,Zhang, Dong,Jiao, Jing-Hua,Wang, Yu,Wu, Jing-Yang,Huang, De-Sheng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6

Background: The p53-binding protein 1 (TP53BP1) gene may be involved in the development of cancer through disrupting DNA repair. However, investigation of associations between TP53BP1 rs2602141 A/C polymorphism and cancer have yielded contradictory and inconclusive outcomes. We therefore performed a meta-analysis to evaluate the association between the TP53BP1 rs2602141 A/C polymorphism and cancer susceptibility. Materials and Methods: Published literature from PubMed, Medline, the Cochrane Library, EMbase, Web of Science, Google (scholar), CBMDisc, Chongqing VIP database, and CNKI database were retrieved. Pooled odds ratios (ORs) with 95% confidence intervals (CIs) were calculated using fixed or random-effects models. Publication bias was estimated using funnel plots, Begg's and Egger's test. Results: A total of seven studies (3,018 cases and 5,548 controls) were included in the meta-analysis. Our results showed that the genotype distribution of TP53BP1 rs2602141 A/C was not associated with cancer risk overall. However, on subgroup analysis, we found that TP53BP1 rs2602141 A/C was associated with cancer risk within an allele model (A vs C, OR=1.14, 95%CI: 1.01-1.29) and a codominant model (AA vs CC, OR=1.36, 95%CI: 1.06-1.74) in Asians rather than in Caucasians. Subgroup analysis by cancer type, genotype, and with or without adjustment for controls showed no significant association. Conclusions: The findings suggested an association between rs2602141 A/C polymorphism in TP53BP1 gene and increased risk of cancer in Asians.