Inheritance of Agronomic Traits and Their Interrelationship in Mungbean(Vigna radiata(L.) Wilczek)

Sriphadet, Sukhumaporn,Lambrides, Christopher J.,Srinives, Peerasak The Korean Society of Crop Science 2007 Journal of crop science and biotechnology Vol.10 No.4

A study was conducted to observe the variation and inheritance of agronomic traits and their interrelationship in mungbean. The objective of the study was to compare agronomic traits and hardseed percentage of 268 recombinant inbred lines(RILs) developed from the cross between wild Vigna subspecies sublobata "ACC 41" with the mungbean cultivar "Berken". The RIL population and their parents were evaluated under controlled conditions in a glass house at the University of Queensland, Brisbane, Australia. The results showed significant differences among the RILs and among the parents in all traits under study. Berken had a longer flowering date and a higher seed weight per plant, but less total leaf number and pod number per plant than ACC 41. A germination test between papers revealed that ACC 41 was 100% hard-seeded and did not germinate at all, while Berken germinated up to 100%. Their RILs distributed well between 0 to 100% hardseed. Upon scarification, all hardseed germinated within seven days. Narrowsense heritability estimates of total leave number, hardseedness, pod length, and pod width were highly heritable at 89.9, 98.9, 93.7, and 93.2%, respectively. The heritability of seed weight per plant and number of seeds per plant were lower at 63.1 and 58.4%, respectively. Seed weight per plant showed positive transgressive segregation when compared with ACC 41 and a positive correlation with 100 seed weight. While the number of seeds per pod showed a negative transgressive segregation when compared with Berken and a negative correlation with pod length and pod width. The RILs gave a 1:1 segregation ratio in leaflet shape, growth habit, and growth pattern, indicating that these traits were controlled by a single dominant gene.

Inheritance of Agronomic Traits and Their Interrelationship in Mungbean (Vigna radiata (L.) Wilczek)

Peerasak Srinives,Sukhumaporn Sriphadet,Christopher J. Lambrides 한국작물학회 2007 Journal of crop science and biotechnology Vol.10 No.4

A study was conducted to observe the variation and inheritance of agronomic traits and their interrelationship in mungbean. The objective of the study was to compare agronomic traits and hardseed percentage of 268 recombinant inbred lines (RILs) developed from the cross between wild Vigna subspecies sublobata “ACC 41” with the mungbean cultivar “Berken”. The RIL population and their parents were evaluated under controlled conditions in a glass house at the University of Queensland, Brisbane, Australia. The results showed significant differences among the RILs and among the parents in all traits under study. Berken had a longer flowering date and a higher seed weight per plant, but less total leaf number and pod number per plant than ACC 41. A germination test between papers revealed that ACC 41 was 100% hard-seeded and did not germinate at all, while Berken germinated up to 100%. Their RILs distributed well between 0 to 100% hardseed. Upon scarification, all hardseed germinated within seven days. Narrowsense heritability estimates of total leave number, hardseedness, pod length, and pod width were highly heritable at 89.9, 98.9, 93.7, and 93.2%, respectively. The heritability of seed weight per plant and number of seeds per plant were lower at 63.1 and 58.4%, respectively. Seed weight per plant showed positive transgressive segregation when compared with ACC 41 and a positive correlation with 100 seed weight. While the number of seeds per pod showed a negative transgressive segregation when compared with Berken and a negative correlation with pod length and pod width. The RILs gave a 1:1 segregation ratio in leaflet shape, growth habit, and growth pattern, indicating that these traits were controlled by a single dominant gene. A study was conducted to observe the variation and inheritance of agronomic traits and their interrelationship in mungbean. The objective of the study was to compare agronomic traits and hardseed percentage of 268 recombinant inbred lines (RILs) developed from the cross between wild Vigna subspecies sublobata “ACC 41” with the mungbean cultivar “Berken”. The RIL population and their parents were evaluated under controlled conditions in a glass house at the University of Queensland, Brisbane, Australia. The results showed significant differences among the RILs and among the parents in all traits under study. Berken had a longer flowering date and a higher seed weight per plant, but less total leaf number and pod number per plant than ACC 41. A germination test between papers revealed that ACC 41 was 100% hard-seeded and did not germinate at all, while Berken germinated up to 100%. Their RILs distributed well between 0 to 100% hardseed. Upon scarification, all hardseed germinated within seven days. Narrowsense heritability estimates of total leave number, hardseedness, pod length, and pod width were highly heritable at 89.9, 98.9, 93.7, and 93.2%, respectively. The heritability of seed weight per plant and number of seeds per plant were lower at 63.1 and 58.4%, respectively. Seed weight per plant showed positive transgressive segregation when compared with ACC 41 and a positive correlation with 100 seed weight. While the number of seeds per pod showed a negative transgressive segregation when compared with Berken and a negative correlation with pod length and pod width. The RILs gave a 1:1 segregation ratio in leaflet shape, growth habit, and growth pattern, indicating that these traits were controlled by a single dominant gene.

Characterization, Inheritance, and Molecular Study of Opaque Leaf Mutant in Mungbean (Vigna radiata (L.) Wilczek)

Rungnoi, Ornuma,Chanprem, Sonthichai,Toojinda, Theerayut,Godwin, Ian,Lambrides, Chris,Srinives, Peerasak 한국작물학회 2010 Journal of crop science and biotechnology Vol.13 No.4

Chlorophyll-deficiency mutants are useful as genetic markers and as materials for studying the photosynthesis process. We characterized the inheritance of the gene controlling an opaque leaf (OL) trait in mungbean (Vigna radiata (L.) Wilczek). An $F_2$ population was developed from the cross between the OL mutant and 'Berken', an Australian mungbean cultivar, to study inheritance and molecular tagging, The population was advanced by selfing to produce $F_5$ lines from which two normal lines and two OL lines were randomly chosen to study chlorophyll content, seed growth and development, and seed cell morphology, The chlorophyll content in opaque leaf was lower than normal and thus was expected to have lower photosynthetic activity. This resulted in yellowish and shrinking pods and seeds within 15 days after flowering, while those from normal plants extended their growth up to 18 days. The cotyledon transfer cells of the OL plants deformed at 12 days and deteriorated at 14 days after flowering. The OL trait was controlled by a single recessive of ol gene which was independent from the genes controlling petiole color and growth habit. We used 193 AFLP primer combinations to tag this gene and found that the marker AGG/ATA was linked with the ol gene at a distance of 3.4 cM.

Characterization, Inheritance, and Molecular Study of Opaque Leaf Mutant in Mungbean (Vigna radiata (L.) Wilczek)

Ornuma Rungnoi,Peerasak Srinives,Sonthichai Chanprem,Theerayut Toojinda,Ian Godwin,Chris Lambrides 한국작물학회 2010 Journal of crop science and biotechnology Vol.13 No.4

Chlorophyll-deficiency mutants are useful as genetic markers and as materials for studying the photosynthesis process. We characterized the inheritance of the gene controlling an opaque leaf (OL) trait in mungbean (Vigna radiata (L.) Wilczek). An F2 population was developed from the cross between the OL mutant and 'Berken', an Australian mungbean cultivar, to study inheritance and molecular tagging. The population was advanced by selfing to produce F5 lines from which two normal lines and two OL lines were randomly chosen to study chlorophyll content, seed growth and development, and seed cell morphology. The chlorophyll content in opaque leaf was lower than normal and thus was expected to have lower photosynthetic activity. This resulted in yellowish and shrinking pods and seeds within 15 days after flowering, while those from normal plants extended their growth up to 18 days. The cotyledon transfer cells of the OL plants deformed at 12 days and deteriorated at 14 days after flowering. The OL trait was controlled by a single recessive ol gene which was independent from the genes controlling petiole color and growth habit. We used 193 AFLP primer combinations to tag this gene and found that the marker AGG/ATA was linked with the ol gene at a distance of 3.4 cM.