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      • KCI등재

        Making an Indian Traditional Rice Variety Mahsuri, Bacterial Blight Resistant Using Marker-Assisted Selection

        Lalitha Shanti Marella,Lalitha Devi Guvvala,Pranitha Koradi,Vinay Shenoy 한국작물학회 2013 Journal of crop science and biotechnology Vol.16 No.2

        Mahsuri a popular traditional variety and the first rain-fed mega variety of the Indian sub- continent. It is highly susceptible to bacterial leaf blight caused by Xanthomonas oryzae pv.oryzae. Nine best performing families of Mahsuri pyramid containing four bacterial blight resistance genes (Xa4, xa5, xa13 and Xa21) were evaluated for agronomic, yield and its related characters viz. days to 50% flowering, plant height, number of tillers, panicle length, filled grains per panicle, grain weight, and yield under natural and disease pressure conditions for three consecutive wet seasons. In addition these pyramids were also evaluated for three different spacings to find out the optimum spacing under disease free and disease pressure conditions. Results revealed that under disease free conditions there was no significant difference between the pyramids and the parent for the characters evaluated in each spacing. However characters plant height, number of tillers, panicle length, filled grains per panicle,yield per plant and yield per sq. m. showed significant variation between the different spacings across seasons. Under heavy disease pressure the parent exhibited highly susceptible reaction whereas the pyramid families were highly resistant. A wider spacing had less yield loss when compared to dense planting under BB infestation in case of parent. There was no such yield loss in the pyramid families. When yield per sq. m. was taken into consideration the 20 x 20 cm spacing showed the highest yield when compared to the other two spacings since number of plants were more. The pyramids insulated the yield loss against bacterial leaf blight and are a gain to the farmers to help overcome the heavy yield losses due to this disease. These pyramids have the potential to replace the parent and can be used directly. In addition they can be used as donors for bacterial blight resistance in any breeding program

      • KCI등재

        Making an Indian Traditional Rice Variety Mahsuri, Bacterial Blight Resistant Using Marker-Assisted Selection

        Guvvala, Lalitha Devi,Koradi, Pranitha,Shenoy, Vinay,Marella, Lalitha Shanti 한국작물학회 2013 Journal of crop science and biotechnology Vol.16 No.2

        Mahsuri a popular traditional variety and the first rain-fed mega variety of the Indian sub-continent. It is highly susceptible to bacterial leaf blight caused by Xanthomonas oryzae pv.oryzae. Nine best performing families of Mahsuri pyramid containing four bacterial blight resistance genes (Xa4, xa5, xa13 and Xa21) were evaluated for agronomic, yield and its related characters viz. days to 50% flowering, plant height, number of tillers, panicle length, filled grains per panicle, grain weight, and yield under natural and disease pressure conditions for three consecutive wet seasons. In addition these pyramids were also evaluated for three different spacings to find out the optimum spacing under disease free and disease pressure conditions. Results revealed that under disease free conditions there was no significant difference between the pyramids and the parent for the characters evaluated in each spacing. However characters plant height, number of tillers, panicle length, filled grains per panicle, yield per plant and yield per sq. m. showed significant variation between the different spacings across seasons. Under heavy disease pressure the parent exhibited highly susceptible reaction whereas the pyramid families were highly resistant. A wider spacing had less yield loss when compared to dense planting under BB infestation in case of parent. There was no such yield loss in the pyramid families. When yield per sq. m. was taken into consideration the $20{\times}20cm$ spacing showed the highest yield when compared to the other two spacings since number of plants were more. The pyramids insulated the yield loss against bacterial leaf blight and are a gain to the farmers to help overcome the heavy yield losses due to this disease. These pyramids have the potential to replace the parent and can be used directly. In addition they can be used as donors for bacterial blight resistance in any breeding program.

      • Estimation of Time Trends of Incidence of Prostate Canner - an Indian Scenario

        Lalitha, Krishnappa,Suman, Gadicherla,Pruthvish, Sreekantaiah,Mathew, Aleyamma,Murthy, Nandagudi S. Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

        Background: With increase in life expectancy, adoption of newer lifestyles and screening using prostate specific antigen (PSA), the incidence of prostate cancer is on rise. Globally prostate cancer is the second most frequently diagnosed cancer and sixth leading cause of cancer death in men. The present communication makes an attempt to analyze the time trends in incidence for different age groups of the Indian population reported in different Indian registries using relative difference and regression approaches. Materials and Methods: The data published in Cancer Incidence in Five Continents for various Indian registries for different periods and/or publications by the individual registries served as the source materials. Trends were estimated by computing the mean annual percentage change (MAPC) in the incidence rates using the relative difference between two time periods (latest and oldest) and also by estimation of annual percentage change (EAPC) by the Poisson regression model. Results: Age adjusted incidence rates (AAR) of prostate cancer for the period 2005-2008 ranged from 0.8 (Manipur state excluding Imphal west) to 10.9 (Delhi) per $10^5$ person-years. Age specific incidence rates (ASIR) increased in all PBCRs especially after 55 years showing a peak incidence at +65 years clearly indicating that prostate cancer is a cancer of the elderly. MAPC in crude incidence rate(CR) ranged from 0.14 (Ahmedabad) to 8.6 (Chennai). Chennai also recorded the highest MAPC of 5.66 in ASIR in the age group of 65+. Estimated annual percentage change (EAPC) in the AAR ranged from 0.8 to 5.8 among the three registries. Increase in trend was seen in the 55-64 year age group cohort in many registries and in the 35-44 age group in Metropolitan cities such as Delhi and Mumbai. Conclusions: Several Indian registries have revealed an increasing trend in the incidence of prostate cancer and the mean annual percentage change has ranged from 0.14-8.6.

      • 판오스 추출물을 이용한 산화 아연 나노파티클의 생합성과 산업용 염료 분해 촉매 활성 및 항균 효과

        Lalitha Kaliraj,Gokulanathan Anandapadmanaban,Ramya Mathiyalagan,Esrat Jahan Rupa,Veronika Soshinikova,안종찬,양동욱,Zuly Elizabeth Jimenez Perez,Josua Markus,양덕춘 한국약용작물학회 2018 한국약용작물학술대회 발표집 Vol.2018 No.10

        Background : Panos extract is a mixture of four Panax plant extracts namely Dendropanax morbifera, Panax ginseng, Acanthopanax senticosus and Kalopanax septemlobus. We intended to use Panos extract for ZnO nanoparticles(NPs) synthesis and application for waste water treatment. Methods and Results : In the present study, we have synthesized Panos ZnO nanoparticles via co precipitation method. Characterization of the NPs has been done using X-ray diffraction (XRD), Fourier transformed infrared spectroscopy (FTIR) and UV-Visible spectroscopy. An average of 75% efficacy in degrading the methylene blue dye has been observed. The nanoparticles showed antibacterial activity against E. coli and S. aureus. Conclusion : The results shows that Panos ZnO NPs can be a potential eco-friendly and economical tool for waste water management in the current scenario where there an intense urge to remediate the polluted environment through novel approaches such as Nanobiotechnology.

      • KCI등재

        The Utility of Endoscopic Ultrasound in Patients with Isolated Elevations in Serum Amylase and/or Lipase

        Lalitha M. Sitaraman,Amit H. Sachdev,Tamas A. Gonda,Amrita Sethi,John M. Poneros,Frank G. Gress 대한소화기내시경학회 2019 Clinical Endoscopy Vol.52 No.2

        Background/Aims: The aim of this study was to describe the diagnostic yield of endoscopic ultrasound (EUS) in patients with isolatedelevated levels of amylase and/or lipase. Methods: A retrospective chart review was conducted at a large academic medical center from 2000 to 2016. Patients were selectedbased on having elevated amylase, lipase, or both, but without a diagnosis of pancreatitis or known pancreatobiliary disease. Patientswere excluded if they had abnormal liver function tests or abnormal imaging of the pancreas. Results: Of 299 EUS procedures performed, 38 met inclusion criteria. Symptoms were present in 31 patients, most frequentlyabdominal pain (87%). In 20 patients (53%), initial EUS most commonly found chronic pancreatitis (n=7; 18%), sludge (5; 13%), or newdiagnosis of pancreas divisum (3; 8%). In the asymptomatic patients (7), 3 had a finding on EUS, most importantly sludge (2), stone (1),and pancreas divisum (1). No patients were diagnosed with a mass or pancreatic cyst. During the follow up period, 6 patients (22%) hadcholecystectomy. Conclusions: In our study of patients with isolated elevations in amylase and/or lipase without acute pancreatitis who underwent EUS,approximately 50% had a pancreatobiliary finding, most commonly chronic pancreatitis or biliary sludge.

      • KCI등재

        Population genetic structure of banana corm weevil Cosmopolites sordidus (Germar) in India

        Lalitha Sunil Kumar,Jyotsna Singh 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.4

        The population genetic structure of Cosmopolites sordidus (Germar), an economically important pest of bananas, was studied using the sequence data of the internal transcribed rDNA (ITS1+ITS2) and the mitochondrial ‘COItRNALeu- COII’ region from seventy nine individuals collected from six sampling locations in India. The ITS data revealed 70% within population variation and non-significant genetic differentiation estimates suggesting lack of phylogeographic sub-structuring. 49% within population variation and highly significant genetic differentiation values were obtained with the mitochondrial data. The Mantel test revealed lack of correlation between genetic and geographic distance with both the markers. Demographic expansion of the populations was confirmed by the star shaped haplotype networks, demographic tests and mismatch distribution curves using both the markers. Molecular diversity indices show a high haplotype diversity (Hd) but low nucleotide diversity (π) suggesting that the populations are closely related. Considering the low self-dispersal ability of the weevils, these results suggest that the range expansion of this banana pest in India has taken place mainly through transport of infested corms and plant material resulting in the weevils forming localised populations which are not genetically distinct from each other. The high gene diversity (Hd) has enabled the weevils to adapt to varying environmental conditions which could explain the range expansion of this pest in India. The observed discrepancy in the genetic differentiation estimates using these two markers can be attributed to the evolutionary dynamics of the nuclear and mitochondrial genomes.

      • Evaluation of Methanolic Extract of Cressa Cretica Linn on Alloxan Induced Hyperglycemic Wistar Rats

        Lalitha Kumari, B. 보안공학연구지원센터 2016 International Journal of Bio-Science and Bio-Techn Vol.8 No.1

        The anti-hyperglycemic effect of methanolic soluble fractions of Cressa cretica (Convolvulaceae) made up of the whole plant (flower, root, leaf, stem) was investigated in alloxan (150 mg/kg) induced hyperglycemic Wistar rats. It’s effect was compared with that of negative control, glibenclamide (0.9 mg/kg) as standard reference drug and Vit-E (100 IU/kg) as anti-oxidant standard. The physico-metabolic parameters measured were: Body weight, glycogen content in liver and muscle, serum glucose, speroxide desmutase (SOD) and histopathological examination of pancreas were also observed. Oral administration of MECC (200 mg/kg, 400 mg/kg) for 28 days exhibited a significant reduction in blood glucose. An improvement of glycogen content in liver and muscle, body weight, hepatic enzyme like SOD was observed. The results of this work suggest that MECC may possess anti-hyperglycemic and anti-oxidant property.

      • KCI등재

        Increased Risk of Pancreatitis after Endoscopic Retrograde Cholangiopancreatography Following a Positive Intraoperative Cholangiogram: A Single-Center Experience

        Lalitha M. Sitaraman,Rita M. Knotts,Judith Kim,Srihari Mahadev,David S. Lee 대한소화기내시경학회 2021 Clinical Endoscopy Vol.54 No.1

        Background/Aims: To determine if patients with a positive intraoperative cholangiogram (IOC) who undergo a subsequentendoscopic retrograde cholangiopancreatography (ERCP) have an increased risk of post-ERCP pancreatitis (PEP) compared to thosewho undergo ERCP directly for suspected common bile duct stones. Methods: A retrospective case-control study was performed from 2010 to 2016. Cases included inpatients with a positive IOC atcholecystectomy who underwent subsequent ERCP. The control group included age-sex matched cohorts who underwent ERCP forcholedocholithiasis. Multivariate logistic regression was used to assess the association between PEP and positive IOC, adjusting formatching variables and additional potential confounders. Results: Of the 116 patients that met the inclusion criteria, there were 91 women (78%) in each group. Nine patients (7.8%)developed PEP in the IOC group, compared to 3 patients in the control group (2.6%). The use of pancreatic duct stents and rectalindomethacin was similar in both groups. After adjusting for age, sex, total bilirubin levels, and any stent placement, patients with apositive IOC had a significantly increased risk of PEP (odds ratio, 4.79; 95% confidence interval, 1.05–21.89; p<0.05). Conclusions: In this single-center case-control study, there was a five-fold increased risk of PEP following a positive IOC comparedto an age-sex matched cohort.

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