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Analysis on the Teaching Mode of Tourism Education Based on Internet
Jin Laiyou(金?友) 세계문화관광학회 2008 Conference Proceedings Vol.9 No.0
With development of modem teaching technology and internet, education based on internet has become an effective teaching method and greatly challenged the traditional tourism education mode. But due to fact that it's newly introduced and has only been applied in afew tourism colleges, tourism education based on internet and multimedia has not been fully developed and still needs a lot of improvement. 以互???基?的?育己成??代?展的主?之一,??我???的旅游?育模式提出了挑?,?互??引入旅游?育?系已成??史之必然。由于利用互???展旅游?育在我?起步??,且?施主?多?一些旅游高等院校,?然取得一定成就,但是??上我???段互??旅游?育不均衡,??和多媒???仍?在一?初?的水平,存在着?多?待改?之?。
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Wang Laiyou,Guo Shuxian,Zeng Bo,Wang Shanshan,Chen Yan,Cheng Shuang,Liu Bingbing,Wang Chunyan,Wang Yu,Meng Qingshan 한국균학회 2022 Mycobiology Vol.50 No.1
The identification of oleaginous yeast species capable of simultaneously utilizing xylose and glucose as substrates to generate value-added biological products is an area of key eco- nomic interest. We have previously demonstrated that the Cutaneotrichosporon dermatis NICC30027 yeast strain is capable of simultaneously assimilating both xylose and glucose, resulting in considerable lipid accumulation. However, as no high-quality genome sequenc- ing data or associated annotations for this strain are available at present, it remains chal- lenging to study the metabolic mechanisms underlying this phenotype. Herein, we report a 39,305,439 bp draft genome assembly for C. dermatis NICC30027 comprised of 37 scaffolds, with 60.15% GC content. Within this genome, we identified 524 tRNAs, 142 sRNAs, 53 miRNAs, 28 snRNAs, and eight rRNA clusters. Moreover, repeat sequences totaling 1,032,129 bp in length were identified (2.63% of the genome), as were 14,238 unigenes that were 1,789.35 bp in length on average (64.82% of the genome). The NCBI non-redundant protein sequences (NR) database was employed to successfully annotate 11,795 of these unigenes, while 3,621 and 11,902 were annotated with the Swiss-Prot and TrEMBL databases, respectively. Unigenes were additionally subjected to pathway enrichment analyses using the Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Cluster of Orthologous Groups of proteins (COG), Clusters of orthologous groups for eukaryotic com- plete genomes (KOG), and Non-supervised Orthologous Groups (eggNOG) databases. Together, these results provide a foundation for future studies aimed at clarifying the mech- anistic basis for the ability of C. dermatis NICC30027 to simultaneously utilize glucose and xylose to synthesize lipids.
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Event-triggered Control of Positive Systems With State Saturation Using Linear Programming
Yu Shao,Junfeng Zhang,Laiyou Liu,Xuanjin Deng 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.1
This paper proposes the event-triggered control of positive systems with state saturation both in discretetime and continuous-time cases. A 1-norm based event-triggered mechanism is established for positive systems. Under the event-triggered mechanism, the error term between actual and sample states is transformed into intervaluncertain form. Together with the properties of saturation, the systems with state saturation are transformed intointerval uncertain systems and the corresponding lower and upper bounds of the system matrices are obtained,respectively. Using a linear co-positive Lyapunov function, the event-triggered controller and the auxiliary gainmatrix of the domain of attraction are designed in terms of linear programming, respectively. Then, the systemswith state and input saturation are also described via interval uncertain systems. An event-triggered controller isdesigned and thus the closed-loop systems are positive and stable under the designed controller. Furthermore, thepresented event-triggered control approach is extended to the continuous-time case. Compared to existing controlapproaches, the event-triggered control can reduce energy consumption and increase the practicability. Finally,several numerical examples are given to illustrate the effectiveness of the proposed design
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Gene Expression Profiling in the Pituitary Gland of Laying Period and Ceased Period Huoyan Geese
Luan, Xinhong,Cao, Zhongzan,Xu, Wen,Gao, Ming,Wang, Laiyou,Zhang, Shuwei Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.7
Huoyan goose is a Chinese local breed famous for its higher laying performance, but the problems of variety degeneration have emerged recently, especially a decrease in the number of eggs laid. In order to better understand the molecular mechanism that underlies egg laying in Huoyan geese, gene profiles in the pituitary gland of Huoyan geese taken during the laying period and ceased period were investigated using the suppression subtractive hybridization (SSH) method. Total RNA was extracted from pituitary glands of ceased period and laying period geese. The cDNA in the pituitary glands of ceased geese was subtracted from the cDNA in the pituitary glands of laying geese (forward subtraction); the reverse subtraction was also performed. After sequencing and annotation, a total of 30 and 24 up and down-regulated genes were obtained from the forward and reverse SSH libraries, respectively. These genes mostly related to biosynthetic process, cellular nitrogen compound metabolic process, transport, cell differentiation, cellular protein modification process, signal transduction, small molecule metabolic process. Furthermore, eleven genes were selected for further analyses by quantitative real-time PCR (qRT-PCR). The qRT-PCR results for the most part were consistent with the SSH results. Among these genes, Synaptotagmin-1 (SYT1) and Stathmin-2 (STMN2) were substantially over-expressed in laying period compared to ceased period. These results could serve as an important reference for elucidating the molecular mechanism of higher laying performance in Huoyan geese.
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Yufei Tian,Wei Yang,Gang Chen,Chuanlong Men,Yifan Gu,Ruilin Zhang,Laiyou Wang,Xin Zhang 한국식품과학회 2022 Food Science and Biotechnology Vol.31 No.2
Currently, gut microbiota living in the gastrointestinaltract, plays an important role in regulatinghost’s sleep and circadian rhythms. As a tool, gut microbiotahas great potential for treating circadian disturbanceand circadian insomnia. However, the relationship betweengut microbiota and circadian rhythms is still unclear, andthe mechanism of action has still been the focus ofmicrobiome research. Therefore, this article summarizesthe current evidences associating gut microbiota with factorsthat impact host circadian rhythms neurology sleepdisorder. Moreover, we discuss the changes to these systemsin sleep disorder and the potential mechanism ofintestinal microbiota in regulating circadian rhythms neurologysleep disorder via microbial metabolites. Meanwhile,based on the role of intestinal flora, it is provided anovel insight into circadian related insomnia and will bebenefit the dietary treatment of circadian disturbance andthe circadian related insomnia.
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