Humic Acid Confers HIGH-AFFINITY K+ TRANSPORTER 1-Mediated Salinity Stress Tolerance in Arabidopsis

Laila Khaleda,박희진,윤대진,전종록,김민갑,차준영,김외연 한국분자세포생물학회 2017 Molecules and cells Vol.40 No.12

Excessive salt disrupts intracellular ion homeostasis and inhibits plant growth, which poses a serious threat to global food security. Plants have adapted various strategies to survive in unfavorable saline soil conditions. Here, we show that humic acid (HA) is a good soil amendment that can be used to help overcome salinity stress because it markedly reduces the adverse effects of salinity on Arabidopsis thaliana seedlings. To identify the molecular mechanisms of HA-induced salt stress tolerance in Arabidopsis, we examined possible roles of a sodium influx transporter HIGH-AFFINITY K+ TRANSPORTER 1 (HKT1). Salt-induced root growth inhibition in HKT1 overexpressor transgenic plants (HKT1-OX) was rescued by application of HA, but not in wild-type and other plants. Moreover, salt-induced degradation of HKT1 protein was blocked by HA treatment. In addition, the application of HA to HKT1-OX seedlings led to increased distribution of Na+ in roots up to the elongation zone and caused the reabsorption of Na+ by xylem and parenchyma cells. Both the influx of the secondary messenger calcium and its cytosolic release appear to func-tion in the destabilization of HKT1 protein under salt stress. Taken together, these results suggest that HA could be applied to the field to enhance plant growth and salt stress tolerance via post-transcriptional control of the HKT1 transporter gene under saline conditions.

Production and characterization of polyclonal antibody against Arabidopsis GIGANTEA, a circadian clock controlled flowering time regulator

Laila Khaleda,차준영,김민갑,김외연 한국식물학회 2017 Journal of Plant Biology Vol.60 No.6

Arabidopsis GIGANTEA (GI) is encoded by asingle gene and highly conserved among vascular plants andits mutants display pleiotropic phenotypes involved indiverse biological processes such as light signaling, circadianclock, and sucrose metabolism as well as abiotic stressresponses. However, molecular mechanisms of GI arelargely unknown due to the lack of useful antibody. To date,the epitope tags have been widely used to detect GI in plants,but it needs to generate the transgenic plants which take afew months. Here, we produced polyclonal α-GI antibodyusing truncated variants of GI having amino-terminal (1-858aa) and carboxyl-terminal (920-1173) regions as antigens. Both recombinant His-GI1-858 and His-GI920-1173 proteins wereindividually and successfully expressed in E. coli andimmunized into rabbit. Anti-serum was purified by antigenspecificaffinity purification method using both recombinantHis-GI1-858 and His-GI920-1173 proteins. Purified polyclonal α-GI antibody not only detected endogenous GI proteins inwild-type Arabidopsis plants, but also reenacted its dieloscillations. Furthermore, the antibody showed cross-reactivitywith the GI orthologs in other plants such as Chinesecabbage, rape and tomato. Our polyclonal GI antibody couldhelp to determine the molecular mechanisms of GI involvedin largely unknown pleiotropic responses in plants.

EU-ASEAN Proposed FTA

Laila Arjuman Ara 한국외국어대학교 인도연구소 2014 남아시아연구 Vol.20 No.1

Various ASEAN member states including Singapore, Vietnam, and Thailand are negotiating bilateral Free Trade Agreement(FTAs) with the EU. Recently, Myanmar has received EBA from the EU. Most of the ASEAN countries are main competitors of Bangladesh in the EU market. The main objective of the study is to investigate the potential economic impacts on Bangladesh of the proposed EU?ASEAN free trade agreement. In this context, a Computable General Equilibrium(CGE) analysis has been developed by using the Global Trade Analysis Project(GTAP) model and database to explore the aggregate impact of the proposed FTA. The CGE analysis evinces that if EUASEAN signs FTA, Bangladesh would experience a fall in real GDP of 0.13 per cent and welfare losses would be USD 22 million. Bangladesh’s export could also fall by about 0.16 percent to the EU market. This study also shows that if the EU and Vietnam sign an FTA, Bangladesh’s exports to the EU are expected to drop by about 0.04 per cent and the welfare loss equals USD 5 million. Bangladesh would experience a fall in real GDP by 0.03 per due to sharp deterioration of terms of trade. This suggests that if EU?ASEAN signs an FTA, Bangladesh will face formidable market access difficulties in the EU.

Detection of Ribosomal DNA Sequence Polymorphisms in the Protist <i>Plasmodiophora brassicae</i> for the Identification of Geographical Isolates

Laila, Rawnak,Robin, Arif Hasan Khan,Yang, Kiwoung,Choi, Gyung Ja,Park, Jong-In,Nou, Ill-Sup MDPI 2017 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.18 No.1

<P>Clubroot is a soil-borne disease caused by the protist <I>Plasmodiophora brassicae</I> (<I>P. brassicae</I>). It is one of the most economically important diseases of <I>Brassica rapa</I> and other cruciferous crops as it can cause remarkable yield reductions. Understanding <I>P. brassicae</I> genetics, and developing efficient molecular markers, is essential for effective detection of harmful races of this pathogen. Samples from 11 Korean field populations of <I>P. brassicae</I> (geographic isolates), collected from nine different locations in South Korea, were used in this study. Genomic DNA was extracted from the clubroot-infected samples to sequence the ribosomal DNA. Primers and probes for <I>P. brassicae</I> were designed using a ribosomal DNA gene sequence from a Japanese strain available in GenBank (accession number AB526843; isolate NGY). The nuclear ribosomal DNA (rDNA) sequence of <I>P. brassicae</I>, comprising 6932 base pairs (bp), was cloned and sequenced and found to include the small subunits (SSUs) and a large subunit (LSU), internal transcribed spacers (ITS1 and ITS2), and a 5.8s. Sequence variation was observed in both the SSU and LSU. Four markers showed useful differences in high-resolution melting analysis to identify nucleotide polymorphisms including single- nucleotide polymorphisms (SNPs), oligonucleotide polymorphisms, and insertions/deletions (InDels). A combination of three markers was able to distinguish the geographical isolates into two groups.</P>

Effect of Roll Gap Change of Oval Pass on Interfacial Slip of Workpiece and Roll Pressure in Round-Oval-Round Pass Rolling Sequence

Laila Salah Bayoumi,Young Seog Lee,Hong Joon Kim 대한기계학회 2002 KSME International Journal Vol.16 No.4

Biochemical Characterization of a Novel Alkaline and Detergent Stable Protease from Aeromonas veronii OB3

( Laila Manni ),( Asmae Misbah ),( Nouhaila Zouine ),( Samir Ananou ) 한국미생물 · 생명공학회 2020 한국미생물·생명공학회지 Vol.48 No.3

An organic solvent- and bleach-stable protease-producing strain was isolated from a polluted river water sample and identified as Aeromonas veronii OB3 on the basis of biochemical properties (API 20E) and 16S rRNA sequence analysis. The strain was found to hyper-produce alkaline protease when cultivated on fish waste powder-based medium (HVSP, 4080 U/ml). The biochemical properties and compatibility of OB3 with several detergents and additives were studied. Maximum activity was observed at pH 9.0 and 60℃. The crude protease displayed outstanding stability to the investigated surfactants and oxidants, such as Tween 80, Triton X-100, and H₂O₂, and almost 36% residual activity when incubated with 1% SDS. Remarkably, the enzyme demonstrated considerable compatibility with commercial detergents, retaining more than 100% of its activity with Ariel and Tide (1 h, 40℃). Moreover, washing performance of Tide significantly improved by the supplementation of small amounts of OB3 crude protease. These properties suggest the potential use of this alkaline protease as a bio-additive in the detergent industry and other biotechnological processes such as peptide synthesis.

Gene diversity-mediated characterization of biological features in entomopathogenic Beauveria bassiana

Laila Gasmi,Sehyeon Baek,Jong Cheol Kim,Mi Rong Lee,Jae Su Kim 한국응용곤충학회 2018 한국응용곤충학회 학술대회논문집 Vol.2018 No.10

Beauveria comprises entomopathogenic fungi frequently isolated from insect cadavers, among which Beauveria bassiana is the most widely studied species of entomopathogenic fungal genus for its high potential as a biological pesticide. Even though it has been reported that B. bassiana is a heterogeneous assemblage of strains, little is known about the factors that might drive the genetic diversity among various isolates. In this work, we ought to study the gene diversity of 33 isolates in order to figure out the relationship between their gene diversity and biological features. First, we analyzed gene sequences that are involved in developmental and immunological processes of the fungal isolates and host. Now we are checking whether the gene diversity is related to the genes function, the isolates geographic localization, thermo-tolerance and virulence of the isolates. The obtained results so far showed no correlation between the genetic diversity and the fungal geographic localization. But interestingly, a positive correlation has been obtained between the virulence and the genetic diversity based on the internal transcribed spacers (ITS). Further analyses are aiming to confirm the obtained data and to analyze possible correlations between the genetic diversity and morphological and transcriptional data of the studied isolates. Based on the obtained results, we could much strongly focus on interesting genes which might be related to improving or screening highly virulent fungal agents as biological control agents.

Gene diversity-mediated characterization of biological features in entomopathogenic Beauveria bassiana

Laila Gasmi,Sehyeon Baek,Jong Cheol Kim,Mi Rong Lee,Jae Su Kim 한국농약과학회 2018 한국농약과학회 학술발표대회 논문집 Vol.2018 No.10

Analysis of Billet Rolling in a Continuous Mill using Idle Vertical Stands

Laila S.Bayoumi,Youngseog Lee 대한기계학회 2004 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.18 No.5

An analytical approach is presented to investigate the deformation characteristics of billets in a continuous billet mill using power driven horizontal stands and idle vertical stands. The analysis is validated by comparison to the experimental results in a previously published work. The analytical results have shown that, apart from the problems of slip and buckling of billet, there are some shortcomings involved in this method. Compared to conventional rolling with all driven stands, the roll load for idle vertcal stands and the rolling torque for horizontal stands are almost doubled. The billet is severely stressed within the roll-bite of idle vertical stands and the overall rolling power has increased by one third of that for conventional roling. These shortcomings impair the feasibility of industrial application of idle vertical stand rolling method.<br/>

Cervical cancer prevention program in Jakarta, Indonesia: See and Treat model in developing country

Laila Nuranna,Mohamad Farid Aziz,Santoso Cornain,Gatot Purwoto,Sigit Purbadi,Setyawati Budiningsih,Budiningsih Siregar,Alexander Arnold Willem Peters 대한부인종양학회 2012 Journal of Gynecologic Oncology Vol.23 No.3

Objective: The purpose of this study was to describe the implementation of single visit approach or See-visual inspection of the cervix with acetic acid (VIA)-and Treat-immediate cryotherapy in the VIA positive cases-model for the cervical cancer prevention in Jakarta, Indonesia. Methods: An observational study in community setting for See and Treat program was conducted in Jakarta from 2007 until 2010. The program used a proactive and coordinative with VIA and cryotherapy (Proactive-VO) model with comprehensive approach that consists of five pillars 1) area preparation, 2) training, 3) awareness, 4) VIA and cryotherapy, and 5) referral. Results: There were 2,216 people trained, consist of 641 general practitioners, 678 midwives, 610 public health cadres and 287 key people from the society. They were trained for five days followed by refreshing and evaluation program to ensure the quality of the test providers. In total, 22,989 women had been screened. The VIA test-positive rate was 4.21% (970/22,989). In this positive group, immediate cryotherapy was performed in 654 women (67.4%). Conclusion: See and Treat program was successfully implemented in Jakarta area. The Proactive-VO model is a promising way to screen and treat precancerous lesions in low resource setting. Objective: The purpose of this study was to describe the implementation of single visit approach or See-visual inspection of the cervix with acetic acid (VIA)-and Treat-immediate cryotherapy in the VIA positive cases-model for the cervical cancer prevention in Jakarta, Indonesia. Methods: An observational study in community setting for See and Treat program was conducted in Jakarta from 2007 until 2010. The program used a proactive and coordinative with VIA and cryotherapy (Proactive-VO) model with comprehensive approach that consists of five pillars 1) area preparation, 2) training, 3) awareness, 4) VIA and cryotherapy, and 5) referral. Results: There were 2,216 people trained, consist of 641 general practitioners, 678 midwives, 610 public health cadres and 287 key people from the society. They were trained for five days followed by refreshing and evaluation program to ensure the quality of the test providers. In total, 22,989 women had been screened. The VIA test-positive rate was 4.21% (970/22,989). In this positive group, immediate cryotherapy was performed in 654 women (67.4%). Conclusion: See and Treat program was successfully implemented in Jakarta area. The Proactive-VO model is a promising way to screen and treat precancerous lesions in low resource setting.