CATV 망에서의 고속 DATA 서비스를 위한 적정 셀 규모에 관한 연구

정래성,구대성,김형준,김필중,김종빈 조선대학교 전자정보통신연구소 2001 電子情報通信硏究所論文誌 Vol.4 No.2

Recently the surrounding of data communication industry has been changing through the rapid growth of the information technology, which has been sparked by the convergence of computer and optical fiber technology. This phenomenon requires broadband network to transmit video, audio and data at once. Multimedia and broadband services are provided widely over the Internet. The traditional telephone companies, CATV operators, and Internet service providers are all rushing to provide integrated services over their infra-structures. Among them, CATV seems to be the most promising from an economic perspective, because CATV infrastructure is already in place in a majority of homes and can deliver broadband service without requiring costly upgrade of the existing CATV systems. In this paper, analysis the problem of data services over CATV network, suggest size the proper cell for the high speed data services over CATV network.

白花蛇舌草 메탄올 抽出物의 抗腫瘍 效果 및 抗癌 棋戰에 關한 硏究

魯勳政,文九,文錫哉,元秦熹,文永昊,朴來佶 대한한방종양학회 2000 대한한방종양학회지 Vol.6 No.1

Objectives: This experimental study was carried out to evaluate the effects of aqueous and methanol extracts of Hedyotis diffusa which has long been used for cancer treatment in oriental medicines on the induction of apoptotic cell death in human lymphoid leukemia cell line, HL-60. Methods: Cells were treated with various concentrations (200 to 0.4㎍) and periods (6 to 30 hr) of H_2O and methanol extracts of Hedyotis diffusa. Then, cells were tested for viability by MTT assay. Cells wrere treated with 200㎍/ml of methanol extract fork various periods. Genomic DNA was isolated, separated, on 1.5% agarose gels, stained with ethidium bromide and visualized under UV light. Cells were treated with 200㎍/ml of each extract for 16hr.Then, cells were treated with various doses of each extract for 12 hr and 100㎍/ml of methanol extract for various periods. Lysate from the cells used to measure the activity of caspase-1 and-3 proteases by using fluorogenic peptide substrates including acetyl-YVAD-AMC and acetyl-DEVD-AMC, respectively, Cells were treated with 200㎍/ml of each extract for various periods. Cell lysates were immunoprecipated with anti-JNKl antibodies. The immune complex was reacted with 32^p-ATP and c-Jun as a substrate. The phosphotransferase activity of JNKI was measured by suing Phosphoimage analyzer (Fuji Co., Japan). Nuclear extracts were isolated and incubated with oligonucleotide probe of NF-kB was measured by using EMSA and visualized by PhosphoImage analyzer (Fuji Co, Japan). Cell lysates were prepared and analyzed by Western blotting with anti-Bc12 antibodies and anti-Bax antibodies. Cells were pretreated with various doses of methanol extract for 2 hr. Then, the extract was removed by centrifugation. Cells were resuspended with RPMI-1640 media containing 0.3% agarose, 10% FBS, overlayred onto bottom layer agarose and incubated at CO_2 incubator for 6 days. The number of colony was cunted under light microscopy (×100). Results: The death of HL_60 cells was markedly induced by the addition of methanol extract of Hedyotis diffusa in a dose and time-dependent manners. The apoptotic characteristic ladder pattern of DNA strand break was observed in death of HL_60 cells. In addition, it was shown nucleus chromatin condensation under Hoechst staining. Therefore, Hedyotis diffusa extract-induced death of HL-60 cells is mediated by apoptotic signaling processes. The activity of Caspaxe 3-like proteases remained in a basal level in HL-60 cells treated with aqueous extract of Hedyotis diffusa. However, it was markedly increased in HL-60 cells treated with methanol extract of Hedyotis diffusa. In addition, the phosphotransferase activity of JNKl was increased in HL-60 cells treated with methanol extract of Hedyotis diffusa. Furthermore, the activation of transcriptional activator, NF-kB was markedly induced by methanol extract of Hedyotis diffusa. Anti-apoptotic Bc12 was cleaved into 23Kda fragment by treatment of methanol extract of Hedyotis diffusa. However, expression of proapoptotic Bax protein was increased by treatment of methanol extract of Hedyotis diffusa in a time-dependent manner. Furthermore, methanol extract markedly inhibited the colony forming efficiency of HL-60 cells in semisolid agar culture. Conclusions: Above results suggest that methanol extract of Hedyotis diffusa induces the apoptotic death of human leukemic HL-60 cells via activations of Caspase-3 proteases, JNKI, transcriptional activator NF-κB, In addition, our results also suggest that methanol exthanol extract of Hedyotis diffusa reduces the malignant potential of HL-60 cells via down regulation of colony forming effciency through cleavage of Bc12 as well as induction of Bax.

뇌혈류 측정 기기의 개발

김정래,김정열,김덕원,남문현 대한의용생체공학회 1989 의공학회지 Vol.10 No.1

In this paper, principles of Rheoencephalography(REG) was described and REG was designed and developed. Using the developed instrument, REG waveforms of the left and right brain, REG waveform changes by posture, were observed. It was found that REG waveforms could be observed noninvasively. REG can be easily applied for testing the effect of various drugs related to CBF(Cerebral blood flow) since it is quite senaitive to the relative changes of CBF. Various cerebral diseases can be diagnosed by analyzing the REG waveforms. The area of restricted cerebral blood vessels can be located by arranging the appropriate electrode configuration.

지식 베이스의 에이젠트화에 의한 지식 미디어 시스템에 관한 연구

조정래,손영수,배상현 조선대학교 기초과학연구소 1996 自然科學硏究 Vol.19 No.1

The knowledge of the specialized fields has been changed rapidly in the both side of quantity and quality. A hyper media as a knowledge based system is so fixed in the linked media in mutual that we couldn't tell it information provision with the view of user. In this paper, we propose the way of offering intellectual and flexible information which is same with demand in the side of user, selecting, searching and composing the hypertext at the point of user's view through the design of knowledge media system. three concepts is used in order to challenge the knowledge media system : special document, agent system and ontology. The special document is a knowledge using special fields. The agent system is a homogeneous controller that operates on and application to ensure that its activities are coordinated with those of the others within the community, providing a uniform control mechanism. The last 3th concept, ontology is a language for knowledgement, which is a message exchanged among agents to ensure the proper interaction among them. The combination of these three concepts is used designed the prototype of knowledge media system.

성남지역 종합병원의 의료기기 관리운영 실태에 관한 연구

김정래,이우철 서울보건대학 1996 서울보건대학 부설 병원경영연구소 논문집 Vol.2 No.1

This study was carried out evaluate medical equipment`s management of the general hospital in Seongnam. The purpose of this study was to choose the properly instrument by the effective medical equipment management. Questionaire survey method were used in this study. This results were summerized as follows : Firstly, The result of comparing in this area was present to evalulated normal data more than means of the grobal data. Secondly, medical equipment and articles of consumption management was expressed a few different in each hospital.

McCoy 세포에서 Chlamydia trachomatis의 병원성 인자에 관한 연구

강정숙,최지영,차영주,김영주,김덕례,여명구,박열 조선대학교 생명과학연구소 1998 생명과학 연구 Vol.6 No.-

본 연구는 병원성 세균인 Chlamydia trachomatis(C. trachomatis)가 숙주세포에침습시 C. trachomatis의 병원성 인자와 C. trachomatis와 상호작용하는 숙주 세포막의 인자를 규명함으로써 숙주세포에 대한 감염율을 향상시켜 보다 향상된 진단 방법을 개발하기 위하여 수행하였다. C. trachomatis로 감염된 세포에서 기본소체의 막 단백질을 분리한 결과 각 혈청형의 39 kDa과 42 kDa에서 major outer membranes(MOMPs)이 관찰되었으며, 혈청형 E와 G의 MOMPs는 42.5 kDa으로 동일하였지만 혈청형 F, H, I, 그리고 K의 MOMPs는 각각 다르게 관찰되었다. C. trachomatis가 숙주세포로 침습시 관련된 병원성 인자에 대하여 조사하고자 단층세포로 배양된 배양용기에 C. trachomatis를 24, 48 그리고 73시간대별로 접종시켜 관찰한 결과 72시간의 MOMPs의 양이 가장 많이 증가하였으며 이 결과로 MOMPS이 C. trachomatis 병원성에 중요한 작용을 함을 확인할 수 있었다. C. trachomatis가 침습시 숙주세포의 막 단백질 변화양상을 관찰하기 위해 정상세포와 C. trachomatis로 1, 2, 3 그리고 4시간대별로 접종 시간을 달리한 숙주세포의 막 단백질을 분리한 결과 42 kDa의 단백질 양이 Chlamydia에 감염된 세포 내에서 증가됨을 관찰할 수 있었다. 이로 42 kDa의 막 단백질이 침습시 숙주세포 표면에 대한 수용체로서 작용함을 알 수 있었다. The present study was performed to analyze the pathogenic factors involved in the invasion process of Chlamydia trachomatis(C. trachomatis) into McCoy cells. In this study, elementary bodies(EBs) of C. trachomatis serotypes(E, F, G, H, I, K and LGV) could be isolated by the method of Percoll density gradient centrifugation and the pathogens of serotypes were also compared from whole-cell lysates by using sodium dodecyl sulfate-polyacrylamide gel elelectrophoresis. All chlamydial serotypes showed a single predominant protein ranging from 39 to 42 kDa. The major outer membrane proteins(MOMPs) of the E and G serotypes had an identical molecular weight of 42.5 kDa. In contrast, the MOMPs of the F, H, I and K serotypes were showed different molecular weights of MOMPs. By immunoblotting with anti-MOMP antibody it was revealed that the quantify of MOMPs was time-dependently increased when McCoy cells were infected with Chlamydia for 24, 48 and 72 hr. These results indicated that MOMPs are closely related to chlamydial infection into the host cell.

Neuroleptic Malignant Syndrome에 합병되어 나타난 급성 신부전증 1예

채영래,백인호,송정중 大韓神經精神醫學會 1990 신경정신의학 Vol.29 No.4

Neuroleptic malignant syndrome(NMS) is an uncommon but fatal idiosyncratic reaction to neuroleptics. It comprises fever, muscular rigidity, altered consciousness and autonomic dysfunction. The most common serious complications of NMS is rhabdomyolysis, which produces acute myoglobinuric renal failure. The authors experienced a schizophrenic male patient with acute renal failure as a sequela of NMS. The patient was 27-year-old man who had received haloperidol for 9 years. Emergency hemodialysis, hydration and diuretics promoted recovery of impaired renal function. The patient survived the acute episode of NMS without any sequela and resumed his treatment with neuroleptics.

수면 무호흡과 불안장애의 불면증에 대한 다원수면기록

채정호,박원명,채영래,김제헌 大韓神經精神醫學會 1994 신경정신의학 Vol.33 No.2

Objects : The authors studied polysomnographic findings of patients with sleep apnea DIMS (disorders of initiating and maintainig sleep) syndrome and anxiety disorders with insomnia related to another mental disorders. Methods : Eight patients with sleep apnea DIMS syndrome and fourteen patients with anxiety disorder with insomnia related to another mental disorder(nonorganic) were studied using computerized polysomnography. Results : 1) The sleep period time were significantly decreased in anxiety disorder group and the sleep latency was prolonged in these patients. 2) The changes in sleep stage and the number of arousals during sleep period time were more frequent in sleep apneic patients group. And using these variables we could discriminate sleep apneic patients group from subjects with anxiety disorder. 3) The stage 1 sleep was significantly increased in sleep apneic patients. 4) The apnea index in sleep apnea patients was 28.1±25.7. Conclusion : These results suggested that even though there were similiar clinical presentaion such as insomnia, the sleep features of sleep apnea DIMS syndrome and anxiety disorder with insomnia were different. And though there were no instruments to check respiratory flow or movement, it would be possible to discriminate the sleep apnea DIMS syndrome from the anxiety disorder with insomnia by recording the changes in sleep stage and the number of arousals in overnight electroencephalographic procedure.

주파수 위상 변조에 의한 초음파 탐촉자 변위 발생기에 관한 연구 : A Study on Ultrasonic Transducer displacement generator by Frequency Phase modulation

김정래 韓國컴퓨터情報學會 2002 韓國컴퓨터情報學會論文誌 Vol.7 No.3

3kW 태양광발전시스템의 실증시험(Ⅰ)

박정민,조금배,백형래,최창주 조선대학교 에너지.자원신기술연구소 2004 에너지·자원신기술연구소 논문지 Vol.26 No.1

Consider the optimal design for PV system, it is apply to 3㎾ interconnected electric power PV system, that is a point of reference for 3㎾ PV the spread residential section system. Through the driving various practical system, we look into that fbr efficiency of generation and stability of interconnected system. Using obtained data acquisition, It can be suggest that installation and management for system parameter to optimal design maximum generation electric power. PV system have some losses that are variation radiation, shadow, change temperature, unbalanced grid connection, serial circuit loss, MPP deference loss, PCS loss and so forth. Using obtained various performance characteristic result, we can make database in the future, through the this study, we can get the reliance and have regard to spread PV system.