Reduction of d-lactate content in sauerkraut using starter cultures of recombinant Leuconostoc mesenteroides expressing the ldhL gene

Jin, Q.,Li, L.,Moon, J.S.,Cho, S.K.,Kim, Y.J.,Lee, S.J.,Han, N.S. Society for Bioscience and Bioengineering, Japan ; 2016 Journal of bioscience and bioengineering Vol.121 No.5

<P>The n-form of lactate, which causes metabolic stress upon excessive dietary intake, is mainly produced by Leuconostoc sp., the predominant species in sauerkraut. To shift the metabolic flux of D-lactate from pyruvate to L-lactate, we expressed the L-lactate dehydrogenase (ldhL) gene in Leuconostoc mesenteroides ATCC 8293. The IdhL gene from Lactobacillus plantarum was introduced into L. mesenteroides using the shuttle vectors pLeuCM and pLeuCM42. To elevate the expression level of IdhL in L. mesenteroides, the nucleotides for pyruvate kinase promoter were fused to IdhL and cloned into above vectors to construct pLC18pkL and pLC42pkL. As results, introduction of pLC42pkL in L. mesenteroides significantly improved both L-LDH activity and L-lactate productivity during fermentation, decreasing the D-/L-lactate ratio. When used as a starter culture for sauerkraut fermentation, recombinant L. mesenteroides harboring pLC42pkL increased L-lactate concentration and decreased D-lactate concentration compared to the wild type strain. We newly developed a recombinant L. mesenteroides which has high L-lactate dehydrogenase activity and applied this strain to minimize the harmful effect of D-lactate during the sauerkraut fermentation. To the best of our knowledge, we demonstrate for the first time the effective use of recombinant Leuconostoc sp. for quality improvement of fermented foods. (C) 2015, The Society for Biotechnology, Japan. All rights reserved.</P>

Effects of L-tryptophan, Fructan, and Casein on Reducing Ammonia, Hydrogen Sulfide, and Skatole in Fermented Swine Manure

Q.K. Sheng,Z.J. Yang,H.B. Zhao,X.L. Wang,J.F. Guo 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.8

The effects of daily dietary Bacillus subtilis (Bs), and adding L-tryptophan, fructan, or casein to fecal fermentation broths were investigated as means to reduce the production of noxious gas during manure fermentation caused by ammonia, hydrogen sulfide (H2S), and 3-methylindole (skatole). Eighty swine (50.0±0.5 kg) were equally apportioned to an experimental group given Bs in daily feed, or a control group without Bs. After 6 weeks, fresh manure was collected from both groups for fermentation studies using a 3×3 orthogonal array, in which tryptophan, casein, and fructan were added at various concentrations. After fermentation, the ammonia, H2S, L-tryptophan, skatole, and microflora were measured. In both groups, L-tryptophan was the principle additive increasing skatole production, with significant correlation (r = 0.9992). L-tryptophan had no effect on the production of ammonia, H2S, or skatole in animals fed Bs. In both groups, fructan was the principle additive that reduced H2S production (r = 0.9981). Fructan and Bs significantly interacted in H2S production (p = 0.014). Casein was the principle additive affecting the concentration of ammonia, only in the control group. Casein and Bs significantly interacted in ammonia production (p = 0.039). The predominant bacteria were Bacillus spp. CWBI B1434 (26%) in the control group, and Streptococcus alactolyticus AF201899 (36%) in the experimental group. In summary, daily dietary Bs reduced ammonia production during fecal fermentation. Lessening L-tryptophan and increasing fructan in the fermentation broth reduced skatole and H2S.

Effects of L-tryptophan, Fructan, and Casein on Reducing Ammonia, Hydrogen Sulfide, and Skatole in Fermented Swine Manure

Sheng, Q.K.,Yang, Z.J.,Zhao, H.B.,Wang, X.L.,Guo, J.F. Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.8

The effects of daily dietary Bacillus subtilis (Bs), and adding L-tryptophan, fructan, or casein to fecal fermentation broths were investigated as means to reduce the production of noxious gas during manure fermentation caused by ammonia, hydrogen sulfide ($H_2S$), and 3-methylindole (skatole). Eighty swine ($50.0{\pm}0.5kg$) were equally apportioned to an experimental group given Bs in daily feed, or a control group without Bs. After 6 weeks, fresh manure was collected from both groups for fermentation studies using a $3{\times}3$ orthogonal array, in which tryptophan, casein, and fructan were added at various concentrations. After fermentation, the ammonia, $H_2S$, L-tryptophan, skatole, and microflora were measured. In both groups, L-tryptophan was the principle additive increasing skatole production, with significant correlation (r = 0.9992). L-tryptophan had no effect on the production of ammonia, $H_2S$, or skatole in animals fed Bs. In both groups, fructan was the principle additive that reduced $H_2S$ production (r = 0.9981). Fructan and Bs significantly interacted in $H_2S$ production (p = 0.014). Casein was the principle additive affecting the concentration of ammonia, only in the control group. Casein and Bs significantly interacted in ammonia production (p = 0.039). The predominant bacteria were Bacillus spp. CWBI B1434 (26%) in the control group, and Streptococcus alactolyticus AF201899 (36%) in the experimental group. In summary, daily dietary Bs reduced ammonia production during fecal fermentation. Lessening L-tryptophan and increasing fructan in the fermentation broth reduced skatole and $H_2S$.

Several factors affecting hypericin production of Hypericum perforatum during adventitious root culture in airlift bioreactors

Wu, S. Q.,Yu, X. K.,Lian, M. L.,Park, S. Y.,Piao, X. C. POLISH ACADEMY SCIENCES WARSAW 2014 Acta physiologiae plantarum Vol.36 No.4

Hypericum perforatum L. is a traditional medicinal plant for the treatment of depression and wound healing, and hypericin is one of the main effective active substances. To optimize the culture system for producing hypericin in adventitious root, this study used balloon-type airlift bioreactors to investigate the effect of air volume, inoculation density, indole-3-butyric acid (IBA) concentration and methyl jasmonate (MeJA) concentration on hypericin content and productivity during adventitious root culture. Hypericin content and productivity were improved with increasing air volume, and 0.1 vvm (air volume/culture volume/min) was optimal for hypericin production. Inoculation density also had a great effect on hypericin accumulation. Hypericin content and productivity were favorable in an inoculation density of 5.0 g l(-1) and decreased when inoculation densities were lower or higher than 5.0 g l(-1). Furthermore, 1.25 mg l(-1) IBA enhanced hypericin content and productivity, but too low (a parts per thousand currency sign0.50 mg l(-1)) or too high (a parts per thousand yen1.50 mg l(-1)) IBA concentrations decreased hypericin accumulation. MeJA concentration significantly affected biomass accumulation and hypericin production. The biomass decreased and hypericin production increased with increasing MeJA concentration. Optimum hypericin content (1.61 mg g(-1) DW) and productivity (15.57 mg l(-1)) were obtained at 350 mu M MeJA. The hypericin content in bioreactor-grown adventitious roots was lower than in 3-year field-grown plants, but significantly higher than that in in vitro-grown plantlets and 1-year field-grown plants. Thus, the bioreactor culture of adventitious roots can realize rapid and mass production of hypericin in H. perforatum.

2. ABA enhances protein degradation in secescent leaf of Brassica napus L.

Q. Zhang,B. R. Lee,X. L. Wang,T. H. Kim 한국초지조사료학회 2015 한국초지조사료학회 학술대회논문집 Vol.2015 No.11

Fabrication and characterization of fluorohydroxyapatite nanocrystals/poly(d,l-lactide) composite scaffolds

L. Cheng,S.M. Zhang,P.P. Chen,S.L. Huang,L. Liu,W. Zhou,J. Liu,H. Gong,Q.M. Luo 한국물리학회 2007 Current Applied Physics Vol.7 No.s1

Poly(D,L-lactide) (PDLLA)/uorohydroxyapatite nanocrystals (nano-F-HA) porous scaolds were successfully fabricated through asolvent-casting and particulate-leaching technique. Nano-HA/PDLLA scaold and PDLLA scaold were prepared by using the sameprocess for comparison. The structure, phase and morphology of the nanocomposite scaolds were observed by SEM. The results indi-cated that F-HA nanocrystals were homogeneously dispersed in the PDLLA matrix. The porosity of the scaolds was up to 90%, andmacropores and micropores coexisted and interconnected throughout the scaolds. Cell culture experiments demonstrated that the nano-F-HA/PDLLA scaffold had the best adhesion tendency to chondrocytes among the scaffolds investigated.

High Sustained Virologic Response with Daclatasvir plus Asunaprevir in HCV GT-1b Chinese, Korean and Taiwanese without Baseline NS5A Polymorphisms

( F. Mcphee ),( L. Wei ),( Q. Xie ),( Y. Suzuki ),( J. Toyota ),( Y. Karino ),( K. Chayama ),( Y. Kawakami ),( M. L. Yu ),( S. H. Ahn ),( N. Zhou ),( H. Kumada ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1

Aims: Daclatasvir (DCV) plus asunaprevir (ASV) has demonstrated highsustained virologic response (SVR) in HCV genotype (GT-)1b infection.NS5A-Y93H and NS5A-L31 resistance-associated polymorphisms(RAPs) to DCV are known to impact DCV+ASV response in GT-1b-infectedJapanese. The effect of RAPs on SVR at posttreatment week12 (SVR12) to DCV+ASV was explored in mainland Chinese, Korean,and Taiwanese.Methods: Pooled data from 2 studies of DCV (60 mg daily) + ASV(100 mg capsule, twice-daily) for 24 weeks in GT-1b-infected interferon/ribavirin-naive and -experienced patients from mainland China,Korea, and Taiwan. Similar Japanese data (4 studies; n=445) werepooled for comparison. SVR12 with versus without baseline Y93Hand/or L31 RAPs was compared by age (<65 vs ≥65 years), cirrhosisstatus, and baseline HCV-RNA.Results: SVR12 and baseline NS5A sequences were available for 282patients (126 mainland Chinese [45%〕, 80 Koreans [28%〕, 76Taiwanese [27%〕). NS5A-Y93H and/or -L31 RAPs were observed pretreatmentin 8% mainland Chinese, 14% Korean, and 18%Taiwanese patients, compared with 19% in Japanese. SVR12 in allnon-Japanese patients is shown (Figure); rates were broadly similarbetween countries and with Japanese data (Japanese: 96% overallwithout RAPs, 41% with RAPs). Responses were lower among patientswith baseline RAPs. By contrast, SVR12 in patients without RAPs washigh (92-100%), irrespective of cirrhosis, age, or baseline HCV-RNA.Conclusions: At least 95% of HCV GT-1b-infected patients from mainlandChina, Korea or Taiwan without baseline NS5A-Y93H or -L31polymorphisms who had HCV-RNA ≤7 log10 IU/mL achieved SVR12on DCV+ASV, regardless of cirrhosis status and age.

Numerical studies on the effects of the lateral boundary on soil-structure interaction in homogeneous soil foundations

Z. N. Li,Q. S. Li,M. L. Lou 국제구조공학회 2005 Structural Engineering and Mechanics, An Int'l Jou Vol.20 No.4

In this paper, the finite element method is applied to investigate the effect of the lateral boundary in homogenous soil on the seismic response of a superstructure. Some influencing factors are presented and discussed, and several parameters are identified to be important for conducting soil-structure interaction experiments on shaking tables. Numerical results show that the cross-section width L, thickness H, wave propagation velocity and lateral boundaries of soil layer have certain influences on the computational accuracy. The dimensionless parameter L/H is the most significant one among the influencing factors. In other words, a greater depth of soil layer near the foundation should be considered in shaking table tests as the thickness of the soil layer increases, which can be regarded as a linear relationship approximately. It is also found that the wave propagation velocity in soil layer affects the numerical accuracy and it is suggested to consider a greater depth of the soil layer as the wave propagation velocity increases. A numerical study on a soil-structure experimental model with a rubber ring surrounding the soil on a shaking table is also conducted. It is found the rubber ring has great effect on the soil-structure interaction experiments on shaking table. The experimental precision can be improved by reasonably choosing the elastic parameter and width of the rubber ring.

Epitaxial Multiferroic BiFeO3 Thin Films: Progress and Future Directions

Chu, Y. H.,Martin, L. W.,Zhan, Q.,Yang, P. L.,Cruz, M. P.,Lee, K.,Barry, M.,Yang, S. Y.,Ramesh, R. Taylor Francis 2007 Ferroelectrics Vol.354 No.1

<P> We write this article in honor of Professor Vitaly L. Ginzburg, truly the father of the field of ferroelectricity. This article serves as a review of the current state of research pertaining to multiferroic BiFeO3 thin films. In this review we will delve into details of the growth of BiFeO3 thin films and the use of piezoforce microscopy and x-ray reciprocal space mapping to characterize the crystal structure and domain structure of BiFeO3. We will also discuss the use of vicinal and asymmetric substrates to simplify the domain structure in BiFeO3. By simplifying the domain structure we can, in turn, control the ferroelectric switching mechanisms in BiFeO3. Finally we describe the basic ferroelectric properties of BFO films and discuss the critical issues needed to be solved in BiFeO3 films including leakage, complex domain structure, coercivity, and reliability. Such results are promising for continued exploration for detailed multiferroic-coupling studies in the magnetoelectric BiFeO3 system and BiFeO3, in turn, provides a model platform with which to realize the exciting possibility of electrically control magnetism.</P>

Numerical studies on the effects of the lateral boundary on soil-structure interaction in homogeneous soil foundations

Li, Z.N.,Li, Q.S.,Lou, M.L. Techno-Press 2005 Structural Engineering and Mechanics, An Int'l Jou Vol.20 No.4

In this paper, the finite element method is applied to investigate the effect of the lateral boundary in homogenous soil on the seismic response of a superstructure. Some influencing factors are presented and discussed, and several parameters are identified to be important for conducting soil-structure interaction experiments on shaking tables. Numerical results show that the cross-section width L, thickness H, wave propagation velocity and lateral boundaries of soil layer have certain influences on the computational accuracy. The dimensionless parameter L/H is the most significant one among the influencing factors. In other words, a greater depth of soil layer near the foundation should be considered in shaking table tests as the thickness of the soil layer increases, which can be regarded as a linear relationship approximately. It is also found that the wave propagation velocity in soil layer affects the numerical accuracy and it is suggested to consider a greater depth of the soil layer as the wave propagation velocity increases. A numerical study on a soil-structure experimental model with a rubber ring surrounding the soil on a shaking table is also conducted. It is found the rubber ring has great effect on the soil-structure interaction experiments on shaking table. The experimental precision can be improved by reasonably choosing the elastic parameter and width of the rubber ring.