Inheritance of P34 Allergen Protein in Mature Soybean Seed

Mi Kyung Sung,Jun Soo Seo,Kyung Roc Kim,Eun Hui Han,Jin Woo Nam,Dal Soon Kang,Woo Suk Jung,Min Chul Kim,Sang In Shim,Kyung Moon Kim,Jong Il Chung 한국육종학회 2011 한국육종학회지 Vol.43 No.2

Soybean proteins are widely used for human and animal feeds worldwide. The use of soybean protein has been expanded in the food industry due to their excellent nutritional benefits. But, antinutritional and allergenic factors are present in the raw mature soybean. P34 protein, referred as Gly m Bd 30K, has been identified as a predominant immunodominant allergen. The objective of this research is to identify the genetic mode of P34 protein for the improvement of soybean cultivar with a very low level of P34 protein. Two F2 populations were developed from the cross of "Pungsannamulkong" x PI567476 and "Gaechuck2ho" x PI567476 (very low level of P34 protein). Relative amount of P34 protein was observed by Western blot analysis. The observed data for the progeny of "Pungsannamulkong" and PI567476 were 133 seeds with normal content of P34 protein and 35 seeds with very low level of P34 protein (X2=1.157, P=0.20-0.30). For the progeny of "Gaechuck#1" and PI567476, the observed data were 177 seeds with normal content of P34 protein and 73 seeds with very low level of P34 protein (X2=2.353, P=0.10-0.20). From pooled data, observed data were 310 seeds with normal content of P34 protein and 108 seeds with very low level of P34 protein (X2=0.156, P=0.50-0.70). The segregation ratio (3:1) and the Chi-square value obtained from the two populations suggested that P34 protein in mature soybean seed is controlled by a single major gene. Single gene inheritance of P34 protein was confirmed in 32 F2 derived lines in F3 seeds, which were germinated from the low level of P34 protein obtained from the cross of "Pungsannamulkong" and PI567476. These results may provide valuable information to breed for new soybean line with low level of P34 protein and identification of molecular markers linked to P34 locus.

Inheritance of Cgy1 gene and Ti gene in Mature Soybean Seed

Mi Kyung Sung,Eun Hui Han,Kyung Roc Kim,Jung Soo Park,Kyo Jin Hwang,Jin Woo Nam,Jong Il Chung 한국육종학회 2010 한국육종학회지 Vol.42 No.1

Selection of Lx1Lx1lx2lx2lx3lx3 Genotype with Small Seed Size and Black Seed Coat in Soybean

Mi-Kyung Sung,Sang- Bae Seo,Kyung-Roc Kim,Kyoung-Ja Lee,Mo-Se Park,Jong-Il 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10

Lipoxygenase is responsible for the beany flavor and soybean kunitz trypsin inhibitor protein is responsible for the inferior nutritional quality of unheated or incompletely heated soybean meal. Anthocyanins from black soybean seed coat are known to have many pharmaceutical effect. The object of this study is to select black soybean genotype with absence of lipoxygenase-2,3 (Lx1Lx1lx2lx2lx3lx3) and small seed size. Two cultivars (“Seomoktae” and "Gaechuck#1") were used. "Seomoktae" cultivar has the traits of small seed size, black seed coat, green cotyledon and lipoxygenase protein. "Gaechuck#1" has the traits of big seed size, black seed coat, green cotyledon and lipoxygenase free protein. In the F2 population, the several plants with small seed size and lipoxygenase free protein were selected. In F3 and F4 generation, seed size and lipoxygenase protein was comfirmed from the bulked seed harvested at field. The F5 seeds with small seed size and Lx1Lx1lx2llx2lx3lx3 genotype were planted in the field. Several agronomic traits were evaluated. The lines with appropriate agronomic traits were selected.

Selection of soybean Line with small yellow seed coat Lacking Lipoxygenase

Sung Mi Kyung,Kyung Roc Kim,Jung Soo Park,Kyo Jin Hwang,Jong Il Chung 한국육종학회 2009 한국육종학회 학술발표회 발표요지 Vol.41 No.1

Inheritance of P34 allergen protein in mature seed of soybean

Sung Mi Kyung,Kyung Roc Kim,Jung Soo Park,Kyo Jin Hwang,Jun Su Seo,Min Chul Kim,Jong Il Chung 한국육종학회 2009 한국육종학회 학술발표회 발표요지 Vol.41 No.1

커팅 효과가 포함된 디지털 과학 교과서의 설계 및 구현

양현록(Hyun-Roc Yang),강경규(Kyung-Kyu Kang),한광파(Kwang-Pa Han),김동호(Dongho Kim) 한국콘텐츠학회 2009 한국콘텐츠학회논문지 Vol.9 No.1

디지털 시대의 개막과 함께 교육의 패러다임은 변화하고 있다. 이렇게 변화해 가는 패러다임은 더 많은 상호 작용이 존재하는 디지털 교과서 콘텐츠를 필요로 한다. 우리의 목표는 편리한 인터페이스를 갖추고, 커팅효과가 추가되어 기존에 비해 상호작용성이 높은 타블렛 PC 기반의 디지털 교과서를 제작하는 것이다. 이러한 목표를 달성하기 위해서 여러 차례 디지털 교과서를 개발해 온 전문가들과의 회의를 토대로 설계한 인터페이스 및 학습내용에 대해서 설명한다. 그리고 사용자가 임의로 입력한 스트로크에 기반하여 다층 구조의 3D 객체의 단면을 생성하기 위해서 구현된 커팅 알고리즘에 대해서 설명한다. 마지막으로 우리가 개발한 콘텐츠를 시범서비스 했을 때의 결과를 설문 조사 내용을 토대로 하여 상호작용성이 높은 디지털 교과서의 교육적 효과에 대해서 토론할 것이다. The emergence of the digital age has changed the paradigm of education. Recently, the new paradigm needs new digital books that contain more interactive contents. Our goal is to design the digital textbook with convenient interfaces and cutting effects for interactive and effective education. To achieve these goals, we propose interfaces and contents which are designed after a lot of discussion with educational experts. In the implementation step, cutting algorithm is proposed to generate the cut planes of the 3D objects, based on the free strokes specified by the users. In order to test the performance of the contents, the testbed was implemented so that students try our digital book and present their evaluation results on the convenience and the effectiveness.

Independent Inheritance between df2 gene and ti gene in Soybean

Eun Hui Han,Mi Kyung Sung,Kyung Roc Kim,Jung Soo Park,Jin Woo Nam,Jong Il Chung 韓國作物學會 2011 Korean journal of crop science Vol.56 No.1

Dwarfuess and Kunitz trypsin inhibitor (KTI) protein in soybean is useful traits for basic studies. df2 and ti gene control dwarfness and the expression of Kunitz trypsin inhibitor (KTI) protein in soybean, respectively. The objective of this research was to verify genetic linkage or independent inheritance of df2 and ti loci in soybean. The F2 population was made by cross combination between "Gaechuck#2" (Df2Df2titi genotype, KTI protein absence and a normal growth type) and T210 (df2df2TiTi genotype, a dwarf growth type and KTI protein present). A total of 258 F2 seeds were analyzed for the segregation of KTI protein using SDS-PAGE. And so, 198 F2 plants were recorded for the segregation of dwarfness. The segregation ratio of 3 : 1 for Ti locus (201 Ti : 57 titi) and Df2 locus (143 Df2 : 55 df2df2) was observed. Segregation ratio of 9 : 3 : 3 : 1 (116 TiDf2: 44 Tidf2df2: 27 titiDf2: 11 titidf2df2) between df2 gene and ti gene was observed (x2 =3.53, P = 0.223). These results showed that df2 gene was inherited independently with the ti gene in soybean.

Identification of Molecular Markers Linked to the Le gene in Soybean

Mo-Se Park,Kyoung-Ja Lee,Mi-Kyung Sung,Sang-Bae Seo,Kyung-Roc Kim,Jong-Il Chung 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10

Lectin protein is a main antinutritional factor in mature soybean seed. The Le gene controls a lectin protein. Plant breeders can use molecular markers to select indirectly individuals in segregating populations that carry a gene for a favorable trait if a tight linkage exists between a marker locus and the genetic locus controlling that trait. The objective of this research was to identify RAPD markers linked to Le allele using bulked segregant analysis. Cultivar "Gaechuck#2" (LeLe) was crossed with PI548391(lele, absence of lectin protein) and F1 seeds were planted. The F1 plants were grown in the greenhouse to produce F2 seeds. Each F2 seed from F1 plants was analysed electrophoretically to determine the presence of the lectin protein band. F2 individual plants were grown in the greenhouse. Young leaf tissues from each F2 plant were collected. At maturity, single F2 plants were harvested. Random F3 seeds from individual F3 seeds harvested were selected and were used to confirm the presence of the lectin protein band. The dominant and recessive F2 plant leaf bulks consisted with ten F2 individual plants were made. 1,000 Operon random primers were used to screen polymorphic band between dominant and recessive bulk. The presence of lectin protein is dominant to the lack of a lectin protein and lectin protein was controlled by a single locus. A few primers that shows polymorphism in bulked samples were selected and were used to obtain segregating data in F2 individual plants.

핀홀 모델기반 Volume-PIV 개발과 원주 후류 측정

도덕희(Deog Hee Doh),문경록(Kyung Roc Moon),조경래(Gyeong Rae Cho) 한국가시화정보학회 2009 한국가시화정보학회 학술발표대회 논문집 Vol.2009 No.11

A new volumetric PIV technique is constructed and is used for the measurements of a cylinder wake. The measurement system consists of 4 cameras(1024 x 992 pixel. 500fps), a Ar-ion laser, two host computers and a synchronizer. A volumetric light beam is illuminated onto the flow field for spatial visualization. For camera calibration the conventional pin-hole model is used. Particle images are reconstructed based upon the voxels using the MART algorithm. The reconstructed voxel images (tomogram pair) of the two consecutive images then analyzed by means of 3D cross-correlation with an iterative multigrid volume deformation technique, returning the three-component velocity vector distribution over the measurement volume. The Reynolds number for the cylinder wake is 315.

Lipoxygenase 및 Kunitz Trypsin Inhibitor 단백질 결핍 콩 계통 육성

김명식 ( Myung Sik Kim ),성미경 ( Mi Kyung Sung ),서상배 ( Sang Bae Seo ),김경록 ( Kyung Roc Kim ),이경자 ( Kyoung Ja Lee ),박모세 ( Mo Se Park ),정종일 ( Jong Il Chung ) 한국콩연구회 2008 韓國콩硏究會誌 Vol.25 No.1

성숙한 콩 종실에 존재하는 성분 중 비린내를 일으키는 lipoxygenase와 단백질의 분해를 방해하는 효소인 Kunitz trypsin inhibitor (KTI) 단백질이 결핍된 녹색자엽 검정종피 및 노란종피 계통을 육성하기 위해여 진행된 연구에서 얻어진 결과는 다음과 같다. P1 x P2 및 P3 x P4의 교배로부터 얻어진 F2 종자에서 lipoxygenase-2, 3 및 KTI 단백질이 결핍된 종자가 선발되었다. 계통육종법으로 세대를 진전시킨 결과 녹색자엽이고 검정 종피색을 가진 lipoxygenase 및 KTI 단백질 결핍 계통은 생육습성이 직립형이고 신육형은 유한신육형이며 경장은 약 50cm로 나타났다. 종실 크기는 백립중이 약 23.2 g 정도이고 종실모양은 편구형이며 6월 초순 파종시 성숙기가 10월 초순경이었다. 노란종피를 가진 lipoxygenase 및 KTI 단백질 결핍 계통은 배축색이 중간 정도의 자색이며 신육형은 유한신육형이었다. 생육습성은 직립형이고 경장은 약 54cm로 중간 정도이며, 화색은 담자색이며 백립중이 약 24g 정도이고 종실모양은 구형이며 제색은 흑색이었다. Lipoxygenase and Kunitz trypsin inhibitor proteins in mature soybean seed are well known as major anti-nutritional factors. Lipoxygenase is responsible for the beany flavor and soybean Kunitz trypsin inhibitor (KTI) protein is responsible for the inferior nutritional quality of unheated or incompletely heated soybean meal. Anthocyanins from black soybean seed coat are known to have many pharmaceutical effects. The objective of this study was to breed soybean genotype of black coated-seed lacking lipoxygenase-2,3 and KTI protein and to breed soybean genotype of yellow coated-seed lacking lipoxygenase-2,3 and KTI protein. Two breeding populations were developed using four different parents. In F2 seed generations of both populations, F2 seeds deficient in lipoxygenase-2,3 and KTI protein were selected by SDS-PAGE. Selected F2 seeds were planted to advance F2 plant. Traits for seed coat and mature embryo colors were observed on the F3 seeds harvested from each F2 plant. F3 lines were advanced from F3 seeds selected. Plant type, height, and maturity date for each F3 plant within line were observed. After single plant harvesting, seed color and absence of lipoxygenase-2,3 and KTI protein were confirmed on random F4 seed of each F3 plant. Selected lines were advanced to the F6 generation. Maturity date, stem type, seed coat and cotyledon color, seed weight were observed on the new soybean lines with absence of lipoxygenase-2,3 and KTI protein.