Analysis of KAP1 expression patterns and human endogenous retrovirus Env proteins in ovarian cancer

Kyung‑Yoon Jeon,Eun‑Ji Ko,Young Lim Oh,Hongbae Kim,Wan Kyu Eo,김아리,Han Gyu Sun,Meesun Ock,Ki Hyung Kim,Hee‑Jae Cha 한국유전학회 2020 Genes & Genomics Vol.42 No.10

Background Human endogenous retroviruses (HERVs) constitute around 8% of the human genome and have important roles in human health and disease, including cancers. Previous studies showed that HERV envelope (Env) proteins are highly expressed in cancer tissues and co-related with cancer progression. KAP1 has been reported to play a key role in regulating retrotransposons, including HERV-K, through epigenetic silencing. Objective The relationship between KAP-1 and HERV Envs expressions was analyzed only in tumor cell lines and has not yet been studied in cancer tissues. In this study, we analyzed the expression patterns and relationship between KAP1 and HERV Env proteins in ovarian cancer tissues. Method The expression patterns of KAP-1 and HERV Env proteins, including HERV-K and HERV-R, were analyzed in ovarian cancer tissue microarrays that contained 80 surgical specimens, including normal ovary and malignant ovarian cancers. Results The expression of HERV-R Env and KAP1 proteins is signifcantly higher in ovarian cancer compared with normal ovary tissues. However, the expression of HERV-K Env did not change signifcantly in cancer tissues. The expression patterns of HERV-K Env and HERV-R Env signifcantly increased in early stages of cancer and KAP1 expression was higher in certain stage and types of cancers. However, the expression of HERV-K Env, HERV-R Env, and KAP1 did not change in diferent age groups. The correlation between the expression of KAP1 and HERV-Env, including HERV-K and HERV-R, was not signifcantly correlated. Conclusions The results of this study showed that there was no signifcant correlation between the expression of KAP1 and HERV Env proteins in ovarian cancer tissues, unlike studies with cell lines in vitro. These results suggest that the actual expression of HERV Env proteins in ovarian cancer tissues may be regulated through various complex factors as well as KAP1.

Poster Session:PS 0546 ; Nephrology : Body Composition Measurements Using Bioimpedance Analysis in Peritoneal Dialysis Patients are Affected by the Resence of Dialysate

( Kyu Hyang Cho ),( Seok Hui Kang ),( Jong Won Park ),( Kyung Woo Yoon ),( Jun Young Do ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: We report a rare case of CAPD peritonitis caused by Ochrobacterium anthropi. Introductions: Among the continuous ambulatory peritoneal dialysis(CAPD) patients, peritonitis is one of the most commonly taken complications, and also the general cause of dialytic modality exchange to hemodialysis. Usual pathogens of CAPD peritonitis may be bacteria, fungi, mycobacteria. Coagulase negative staphylococci, S. aureus, campylobacter, pseudomonas are common reported pathogens among the bacteria, of CAPD peritonitis cases, meanwhile candida is among fungi. Ochrobacterium anthropi is one species of Brucellaceae, which is rare pathogen of human disease. Case (Methods and results): 73 years old female, who was on CAPD due to diabetic end stage renal disease visited Kyung-Hee University hospital with intermittent abdominal pain. Body fi uid analysis showed increased white blood cell(WBC) count of 26,750/mm3 with her peritoneal fi uid. Culture study with peritoneal fi uid suggested O. anthropi, and DNA sequencing with PCR was consistent with O. anthropi. Intraperitoneal ceftazidime and cefazolin were administrated as empirical antibiotics. Ceftazidime resistance was noted with the result of antibiotics sensitivity test at 7th day of hospitalization, and antibiotics were changed into intraperitoneal gentamicin, which showed sensitivity to the pathogen. CAPD catheter removal and antibiotics re-exchange into imipenem and cefazolin, which were other sensitive antibiotics by the sensitivity test, were done since clinical manifestation and peritoneal fi uid WBC count was repeatedly improved and aggravated. The patient discharged with improved lab test results and resolving clinical symptoms afterward. Conclusions: We presentators report rare case of CAPD peritonitis with pathogen of O. anthropi. The pathogen of the case confi rmed by classical microbiologic, and molecular biologic Methods: The patient was unable to treat only with antibiotics, thus CAPD catheter, which might be act as colonizing source, was removed, and the disease resolved.

HPLC/FLD를 이용한 성별·연령별 혈장 homocysteine 정상치 조사

이경옥,류광석,이규범,이경옥,류광석,이규범 대한임상검사정도관리학회 1999 臨床檢査와 精度管理 Vol.21 No.1

The effect of neuropeptides on secretion of Interleukin-8(IL-8)

Kim, Kyung-Jun,Park, Sang-Hyuk,Choi, Kyoung-Kyu,Park, Sang-Jin 大韓齒科保存學會 2006 Restorative Dentistry & Endodontics Vol.31 No.3

본 연구는 치수조직, 치은, 치주인대로부터 배양된 조직을 SP (Substance P)로 4시간, SP, CGRP (Calcitonin Gene-related Peptide), Tumor necrosis factor-α (TNF-α)로 8시간 자극 후 RNase Protection Assay를 시행하고, IL-8의 분비량을 측정해 다음 결과를 얻었다. 1. IL-8 mRNA는 모든세포에서 발현됐다. 2. IL-8 mRNA 발현은 SP (10^(-5)M)와 SP (10^(-8)M)로 4시간 자극 시 증가되지 않았다. 3. IL-8 mRNA 발현은 SP (10^(-4)M)와 CGRP (10^(-6)M)로 8시간 자극 시 증가되지 않았다. 4. TNF-α (2 ng/㎖) 자극 시, IL-8 mRNA 발현이 증가됐다. 5. 치은 세포를 CGRP (10^(-6)M)로 8시간 자극 시, IL-8 분비량이 증가했다 (p < 0.05). 6. 치주인대 세포를 SP (10^(-4)M)로 8시간 자극 시 IL-8 분비량이 증가했다 (p < 0.05). We investigated the secretion of Interleukin-8 (IL-8) from ginviva and periodontal ligament stimulated with Substance P (SP) and Calcitonin Gene-related Peptide (CGRP). Gingiva (GF), periodontal ligament (PDLF) and pulp (PF) tissues were collected from extracted instact 3rd molars. Cultured cells were stimulated with different concentrations of SP for 4 hrs, and stimulated with SP, CGRP and Tumor Necrosis Factor-α (TNF-α) for 8 hrs. Then RNase Protection Assay was carried out. ELISA was performed using supernatants of stimulated cells for quantitative analysis of IL-8. Results were assessed using supernatants of stimulated cells for quantitative analysis of IL-8. Results were assessed using student t-test with significance of P < 0.05. According to this study, the results were as follows: 1. IL-8 mRNA was detected in all type of cells studied (PF, GF and PDLF). 2. IL-8 mRNA expression was not increased after stimulating 4 hrs with SP (10^(-5)M) and SP (10^(-8)M) compared with Mock stimulation in all type of cells studied. 3. IL-8 mRNA expression was not increased after stimulating 8 hrs with SP (10^(-4)M) and CGRP (10^(-6)M) compared with Mock stimulation in all type of cells studied. 4. TNF-α (2 ng/㎖) increased the expression of IL-8 mRNA in all kind of cells studied. 5. The secretion of IL-8 from GF was increased 8 hrs after the stimulation with CGRP (10^(-6)M) (p < 0.05). 6. The secretion of IL-8 from PDLF was increased 8 hrs after the stimulation with SP (10^(-4)M) (p < 0.05). Calcitonin Gene-related Peptide (CGRP) increased Interleukin-8 (IL-8) which plays an important role in chemotaxis of neutrophil in Calcitonin Gene-related Peptide (CGRP) gingival tissue, whereas Substance P increased the secretion of IL-8 from periodontal ligament.

슬관절 골관절염 증상이 환자에게 미치는 우울 효과

박영규,이윤영,이계우,김정아,윤도경,박승회,조경환,홍명호,박용규 대한노인병학회 2001 대한노인병학회지 Vol.5 No.4

림프종 환자에서의 갈륨, TI-201 그리고 Tc-99m MIBI 섭취의 비교

천경아,조인호,원규장,이경희,이형우,현명수,이재태,이규보 영남대학교 의과대학 2002 Yeungnam University Journal of Medicine Vol.19 No.2

Purpose; Ga-67 scintigraphy has been used for the evaluation of tumors, especially lymphoma. Recently, Tl-201 and Tc-99m MIBI were also used to tumor imaging. Tl-201 and Tc-99m MIBI had better physiologic characteristics than Ga-67, so we studied 32 biopsy proven lymphoma patients (male 24, female 8, mean age 46 years) with Ga-67, Tl-201 or Tc-99m MIBI and compared the scan findings. Materials and Methods: Twenty-three of 32 patients were injected 74-111 MBq (2-3 mCi) of Tl-201, before chemotherapy and imaged with dual-headed SPECT (Prism 2000, Picker, USA) at 30 minutes after injection. Delayed images were obtained after 3 hr in 8 patients. Twenty seven of 32 patients were injected 740 MBq (20 mCi) of Tc-99m MIBI and imaged at 30 minutes after injection. 111-185 MBq (3-5 mCi) of Ga-67 was injected in 12 patients and imaged at 48 and 72 hours after injection. Twenty eight patients were diagnosed as non-Hodgkin's lymphoma and others were Hodgkin's lymphoma. Results: Twenty patients were positive on Tl-201 scan and 3 patients showed negative findings. One of these 3 patients, Tc-99m MIBI and Ga-67 scan were positive. Twenty two patients were positive on Tc-99m MIBI scan and 5 patients showed negative findings. One of these 5 patients, Tl-201 was positive and 2 were positive on Ga-67 scan. Ten of 12 patients showed positive findings on Ga-67 scan. The sensitivity of these agents were 83.3%, 87.0% and 81.5% for Ga-67, Tl-201 and Tc-99m MIBI, respectively. The sensitivity was highest in Tl-201 scan, but there were no significant differences among three tests. In this study, there was no significant difference of uptake ratios between early and delayed images of Tl-201. Conclusion: Scintigraphy with Tl-201 and Tc-99m MIBI in lymphoma patients have similar sensitivity with Ga-67.

Formulation Sequence in Korean TV Talk Shows : Pre-Sequence as Consensual Grounds for Managing Category Work

김규현(Kyu-hyun Kim),서경희(Kyung-Hee Suh) 한국사회언어학회 2018 사회언어학 Vol.26 No.2

Kim, Kyu-hyun & Suh, Kyung-Hee. 2018. “Formulation Sequence in Korean TV Talk Shows: Pre-Sequence as Consensual Grounds for Managing Category Work”. The Sociolinguistic Journal of Korea 26(2). 85~117. From the perspectives of conversation analysis (CA) and membership categorization analysis (MCA), this paper analyzes the formulation sequence in Korean television news interviews and celebrity talk-shows. The analysis shows that the host s formulation is normatively oriented to by the guest as a preliminary action, which projects a range of face-impinging actions, such as challenge, assessment, request, etc. The formulation-confirmation sequence furnishes the host with consensual grounds for embarking on affectively-loaded assessment activities vis-à-vis the guest in his/her own terms. The guest, as the formulation-recipient, may block the host s projected action by using disconfirmation, which points to the contingent nature of the power that the host exercises as the agent of morality. The analysis of the formulation sequence is brought to bear upon the examination of the compositional features of the formulation turn (e.g., sentence-ending suffixes, discourse particle, etc.) and their interactional imports.

Low Density Lipoprotein의 실측치와 계산치에 관한 조사

김언규,정재린,이경옥,서덕규,이규범 대한임상병리사협회 1993 대한임상병리사회지 Vol.25 No.1

대학생들의 침습적/의도적 반추가 생명존중의식을 매개로 자살위험성에 미치는 영향 : 우울 수준 별 차이검증

김춘경(Kim, Choon Kyung),조민규(Cho, Min Kyu) 학습자중심교과교육학회 2020 학습자중심교과교육연구 Vol.20 No.9

본 연구는 대학생들의 우울수준에 따라 침습적 반추와 의도적 반추가 생명존중의식을 매개로 자살위험성에 미치는 영향력을 차이를 파악하고, 자살위험성을 감소시키기 위한 효과적인 방안을 제시하는데 목적이 있다. 이를 위해, 대구 및 경북 지역에 거주하는 대학생 210명을 대상으로 반추, 생명존중의식, 자살위험성, 우울 도구를 활용하여 자료를 수집하였다. 이러한 자료를 토대로 SPSS를 활용하여 우울 수준에 따라 영향력의 차이를 검증하였다. 분석 결과, 첫째, 양육태도, 공감능력, 내재화 및 외현화 문제행동은 애착유형에 따라 유의한 차이를 보였다. 둘째, 반추는 우울 수준에 따라 영향력에서 차이를 보이는 것으로 나타났다. 우울 수준에 따른 인과관계 결과를 구체적으로 살펴보면 다음과 같다. 높은 우울 수준을 가진 대학생들의 침습적 반추는 생명존중의식을 매개로 자살위험성에 유의한 영향을 미치는 것으로 나타났다. 이에 반해, 낮은 우울 수준을 가진 대학생들의 의도적 반추는 생명존중의식을 매개로 자살위험성에 유의한 영향을 미치는 것으로 나타났다. The purpose of this study is to identify the difference in the effect of intrusive/deliberate rumination on suicidal risk through mediation of life respect consciousness according to the depression level of college students, and to suggest an effective way to reduce suicidal risk. For this research, 210 numbers of universtiy student from kyung-buk/daegu province completed questionaires assessing rumination, life respect consciousness and suicidal risk. Based on these data, we used spss to compare the difference of influence among variables according to the level of depression. As a result, first, intrusive/deliberate rumination, life respect consciousness and suicidal risk were significantly different according to the level of depression. Second, rumination showed a difference in influence according to the level of depression. The causal results according to the level of depression are described in detail. Intrusive rumination of college students with high levels of depression had a significant effect on suicide risk through mediation of life respect consciousness. On the contrary, deliberate rumination of college students with low depression level had a significant effect on suicide risk through mediation of life respect consciousness.

선천성 수근관 증후군 1례 보고

이규훈,황치문,김민석,김희상,안경회 大韓再活醫學會 1998 Annals of Rehabilitation Medicine Vol.22 No.3