흉선종 제거후에 발생한 재생 불량성 빈혈 : 증례보고

전원선,이상철,김현정,배상병,김찬규,이남수,박노진,이규택,박성규,홍대식,박희숙,원종호 순천향의학연구소 2007 Journal of Soonchunhyang Medical Science Vol.13 No.2

Thymoma is associated with myasthenia gravis, Pure red cell aplasia, and autoimmune diseases such as autoimmune hemolytic anemia, aplastic anemia, and hypogammaglobulinemia. It's association with aplastic anemia is rare and aplastic anemia appearing after surgical removal of thymoma is especially rare. The authors hereby report a case of aplastic anemia occuring in a patient who was diagnosed with thymoma and myasthenia gravis and had the tumor surgically removed. The patient was treated with anti-thymocyte globulin, cyclosporin, and prednisolone, and showed partial remission with hematologic improvements after 12 months.

Effects of epigallocatechin gallate on CoCl_(2)-induced apoptosis in PC12 cells

Yang, Kyu-Ho,Mo, Hyun-Chul,Choi, Nam-Ki,Kim, Seon-Mi,Kim,Won-Jae 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.1

Neuronal apoptotic events, consequently resulting in neuronal cell death, are occurred in hypoxic/ischemic condition. This cell death has been shown to be accompanied with the production of reactive oxygen species (ROS), which can attack cellular components such as nucleic acids, proteins and phospholipid. However, the underlying mechanisms of apoptosis induced in hypoxic/ischemic condition and its treatment methods are unsettled. Cobalt chloride (CoCl_(2)) has been known to mimic hypoxic condition including the production of ROS. Epigallocatechin gallate (EGCG). a green tea polyphenol, has diverse pharmacologial activities in cell growth and death. This study was aimed to investigate the apoptotic mechanism by CoCl_(2) and effects of EGCG on CoCl_(2)-induced apoptosis in PC12 cells. Administration of CoCl_(2) decreased cell survival in dose- and time-dependent manners and induced genomic DNA fragmentation. Treatment with 100 µM EGCG for 30 min before PC12 cells were exposed to 150 µM CoCl_(2), being resulted in the cell viability and DNA fragmentation being rescued. CoCl_(2) caused morphologic changes such as cell swelling and condensed nuclei, whereas EGCG attenuated morphologic changes by CoCl_(2). EGCG suppressed the apoptotic peak and a loss of Δψ_(m) induced by CoCl_(2). CoCl_(2) decreased Bcl-2 expression but Bax expression was not changed in CoCl_(2)-treated cells. EGCG attenuated the Bcl-2 underexpression by CoCl_(2). CoCl_(2) augumented the cytochrome c release from mitochondria into cytoplasm and increased caspase-8, -9 and caspase-3 activity, a marker of the apoptotic executing stage. EGCG ameliorated the incruement in caspase-8, -9 and -3 activity, and cytochrome c release by CoCl_(2). NAC (N-acetyl-cysteine), a scavenger of ROS, attenuated CoCl_(2)-induced apoptosis in consistent with those of EGCG. These results suggest, that CoCl_(2) induces apoptotic cell death through both mitochondria- and death receptor-dependent pathway and EGCG has neuroprotective effects against CoCl_(2)-induced apoptosis in PC12 cells. 신경세포자멸사는 저산소 및 허혈환경에서 일어나며 이러한 세포죽은 reactive oxidant species (ROS) 생성을 동반함이 알려져있다. 그러나, 저산소 및 허혈환경에서 일어나는 세포자멸사의 기전 및 그 치료방법은 아직 정립되어 있지 않다. CoCl_(2)는 ROS를 생성하는 등 저산소환경과 유사한 조건을 초래하는 것으로 알려져 있다. Epigallocatechin gallate (EGCG)는 녹차의 polyphenol 성분으로서 세포성장과 죽음에 다양한 약리학적 효과를 나타냄이 알려져 있다. 본 연구는 PC12 세포에서 CoCl_(2)에 의한 세포자멸사기전을 밝히고 이에 미치는 EGCG의 효과를 조사하는데 목적이 있다. Cell viability는 MTT 측정으로 조사되었고, DNA fragmentation은 DNA laddering으로 조사되었다. Bcl-2와 Bax발현 정도는 RT-PCR로, caspase-3와 -9의 활성은 spectrophotometer, caspase-8의 활성은 flow cytometry에 의해 측정되었다. 미토콘드리아에서 세포질로 분비된 cytochrome c는 western blot으로, 분해된 DNA 양과 미토콘드리아 세포막전위 (Δψ_(m))는 FACScan으로 조사되었다. CoCl_(2)투여로 PC12 세포수는 용량 및 시간 의존형태로 감소하였고, genomic DNA fragmentation이 발생하였다. CoCl_(2)투여로 야기된 cell viability의 감소와 DNA fragmentation은 EGCG 전처치에 의해 억제되었다. CoCl_(2)은 세포용적팽창과 condensed nuclei 같은 형태적 변화를 일으켰으며, apoptotic peak, Δψ_(m)감소 및 cytochrome c 유리를 야기하였다. EGCG는 CoCl_(2)에 의한 세포형태변화, apoptotic peak, Δψ_(m)소실 및 cytochrome c 유리를 억제시켰다. CoCl_(2)는 Bcl-2 발현을 감소시켰지만, Bax 발현에는 영향을 미치지 않았다. EGCG는 CoCl_(2)에 의해 야기된 Bcl-2 발현 감소를 억제시켰다. CoCl_(2)는 caspase-3, -8, 그리고 -9의 활성을 증가시켰으며, EGCG는 CoCl_(2)에 의한 세포자멸사를 억제시켰다. 본 실험결과는 PC12 세포에서 CoCl_(2)가 미토콘드리아 의존 및 death receptor의존 기전으로 세포자멸사를 일으키며, EGCG는 세포자멸사기전을 억세지킴으로 신경보호기능을 가짐을 시사하였다.

흰쥐 심장혈관계통에서 3β-hydroxysteroid dehydrogenase/△⁵-△⁴ isomerase의 분포에 관한 연구

오선희,오재민,백용아,박옥규,정연태 대한해부학회 1988 Anatomy & Cell Biology Vol.31 No.3

한국 성장기 아동의 Ⅲ급 부정교합 양상에 관한 두부방사선 계측학적 연구

양규호,김선희 大韓小兒齒科學會 1995 大韓小兒齒科學會誌 Vol.22 No.1

This study was performed to analyse the pattern of the Class Ⅲ malocclusion in Korean growing children. The cephlometric radiograms were taken from the growing children at 8 to 12 years old, then 11 items were statistically analysed. The results of this study were as follows : 1. As compared 11 items between Class Ⅲ malocclusion and normal occlusion, there was significant difference on SNA angle, Maxillary incisor to point A(mm), Maxillary incisor-NA line(angle), Maxillary incisor to NA line(mm), IMPA, Facial angle and Pog to Nasion Perpendicular(mm) between Class Ⅲ malocclusion and onrmal occlusion. 2. The most common Class Ⅲ malocclusion pattern was found that the normal maxilla and madible followed by normal maxilla and prognathic mendible. 3. In vertical component of Class Ⅲ malocclusion, subjects with long lower facial height were 54% of the total but with short lower facial height were 10%.

한국 유치원에서의 통일교육: 유치원 교육과정 및 교재 분석

서규선,조경희 서원대학교 교육연구소 1995 敎育發展 Vol.1995 No.14

The first purpose of the study is to analysis the curriculum of our kindergarten Unification Education from the 1945 Liberation of Korea to now. The second purpose of the study is to analysis the texts used for unification Education in our Kindergartens. The results of this study are as follows. 1.The curriculum of Kindergarten Unification Education was developed later then that of primary or secondary school. Kindergarten curriculum of Unification Education has been dealt with only secondary by the authorities and others. 2.There were no texts in this area till 1970. It was 1984 that the first teaching materials of Unification Education for chilern was published. 3.Most of texts used for Unification Education in Kindergartens are not suitable to childern' intellectual and affective developing level. But things have changed greatly in recent years. 4.Man-cented illustrations of theaching materials used for Unification Education in Kindergartens are overwhelmingly lager in number then female-centered illustrations. This show that our society is man-centered one. 5.Cramming method is used mostly to teach the text of our Unification Education in Kindergarten

心理學에 있어서 體系思考의 活用

徐圭善 서원대학교 학생생활연구소 1989 학생생활연구 Vol.7 No.-

Systems is a word of very wide application. It is used in astronomy, physics, biology, ecology, political science, economics and sociology. This paper is about the application of systems thinking in psychology. We examine various conceptual ways of approaching the study of systems and then attempt to integrate psychology and systems. Most of the ideas fundamental for systems thinkers originally were created in other fields such as physics, biology, chemistry, cybernectics etc. The ideas surveyed in these fields provoked psychologists who have a systems orientation to develop new ways of conducting psychological research and practice. While systems thinking historically has been influential within many of the subdisciplines of psychology, five branches of psychology stand out as having fundamental reliance on a systems orientation. These are Gestalt psychology, transactional functionalism, field theory, and community psychology and family therapy. The most contemporary of these are family therapy and community psychology. A definition of systems psychology follows consideration of each of the fundamental postulates. These basic perspectives derive from consideration of the crucial issues of language, epistemology, linearity, causality, forms of reasoning, whole, patterns and rules, discontinuous change. In the practical, applied sense, systems psychology has meant engineering psychology and human factors. Engineering psychology is concerned mainly with the design of equipment, facilities, and environment to match the capabilities and limitations of people. Human factors groups were found, grew, and proliferated in numerous military and industrial organizations.

Real-time PCR을 이용한 치주질환 원인균의 정량적 분석

김선미,양규호,최남기,강미선,오종석 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.3

Real-time PCR 기법을 이용하여 구강내 치태에서 특정 세균의 존재여부를 알 수 있을 뿐 아니라 정량적 분석이 가능하게 되었다. 이 연구는 real-time PCR 기법을 이용하여 8-18세의 청소년에서 치주질환 원인균의 조성을 알아보고자 전신질환이 없는 어린이 및 청소년 65명을 대상으로 치은연하의 치태를 채취하여 5종의 치주질환 원인균의 출현율과 그 양을 측정하고,또한 함께 측정한 치태지수 및 치은지수와의 상관관계를 조사하여 다음과 같은 결론을 얻었다. 1. 치태지수의 평균은 1.33이었으며,치은지수의 평균은 0.97이었다. 치태지수는 나이가 증가할수록 낮아졌다(p<0.05). 2. 치주질환 원인균의 출현율은 P. gingivalis 61.5%, T. forsythia 53.8%, T. denticola 29.2%, A. actinomycetemcomitans 15.4%. F. nucleatum은 100%였다. P. gingivalis, T. denticola는 10세 이후 출현율이 증가하였고 A. actinomycetemcomitans는 8-10세 군에서 출현율이 높았다(p<0.05). 3. 정량적 분석에서 치태지수와 치은지수는 F. mucleatum의 양과 유의한 상관성이 있었으며,치태지수는 치은지수와 유의한 상관성이 있었다. T. forsythia는 A. actinomycetemcomitans, T. denticola양과 강한 상관성을 보였으며, T. denticola양과 A. actinomycetemcomitans도 강한 상관관계를 가지고 있었다. The detection of special bacteria of oral plaque and quantitative analysis are possible through the real-time PCR technique. The aim of this study was to investigate the prevalence and the amount of five periodontopathic bacteria using real-time PCR, and evaluate the relationship among amount of bacteria, plaque index and gingival index. Sixty five subjects ranging from 8 to 18 years old were enrolled. Plaque index and gingival index were examined and five pehodontopathic bacteria were surveyed in subgingival plaque samples. The results were analyzed by ANOVA and Pearson's correlation. Results: The prevalence of P. gingivalis was 61.5%, T. forsythia was 53.8%, T. denticola was 29.2%, A.actinomycetemcomitans was 15.4% and F. nucleatum was 100%. Plaque index and gingival index have a significant relationship with the amount of F. nucleatum. The amount of T,forsythia has a strong relationship with the amount of T, denticola and A, actinomycetemcomitans(p<.0.05).

子宮內膜搔爬術 21例의 鍼麻醉效果에 대한 臨床的 硏究

金在圭,金相佑,李宜暻,金建植,金相佑,南相永 경희대학교 1997 慶熙醫學 Vol.13 No.1

성인 자원자에서 B방식 초음파를 이용해 측정한 경동맥 내막중막두께의 분포

이선규,황희영,김형식,장미선,이은주,강문호,고광곤 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.11

윤리과 교원 연수 프로그램의 현황과 실태

서규선 서원대학교 2004 敎育發展 Vol.23 No.2

According to a current curriculum, the subject name of 'Ethics' must be called 'Morals'. The teacher education is composed with a teacher training and a teacher development. Teacher's researching and training is divided into those for qualification, duty, special task, disposition and self-development. Here we try to observe the present situation of training and development programs for 'Ethics Education' teachers by the educational institution. Currently the important agency for teacher education are centers for in-service secondary education by the college of education, educational training center for teachers by each city and county, distant teacher education center, and optional group by civil society etc. The agencies which take charge of the training and development programs for 'Ethics Education' teachers are disordering too much. Contents and operating method of the teacher education programs have not a consistency, a connection and a discrimination. 'Ethics' teachers is ardently attached not to the effect of teacher education programs but to the score too much. It is the actual condition where generally the system of the programs of Ethics teacher education lacks. The training and development programs for 'Ethics' teachers are changing. The cause of this change is the fact that our society becomes an information society and that the global village become one. The information society changes the organization, program, operation, evaluation and feedback of the agency which takes charge of a teacher education. When the teacher education agency is not adapted to the change, it means the function will be paralyzed. Considering global village, the 'Ethics' teachers must participate in overseas special research programs.