Effects of epigallocatechin gallate on CoCl_(2)-induced apoptosis in PC12 cells

Yang, Kyu-Ho,Mo, Hyun-Chul,Choi, Nam-Ki,Kim, Seon-Mi,Kim,Won-Jae 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.1

Neuronal apoptotic events, consequently resulting in neuronal cell death, are occurred in hypoxic/ischemic condition. This cell death has been shown to be accompanied with the production of reactive oxygen species (ROS), which can attack cellular components such as nucleic acids, proteins and phospholipid. However, the underlying mechanisms of apoptosis induced in hypoxic/ischemic condition and its treatment methods are unsettled. Cobalt chloride (CoCl_(2)) has been known to mimic hypoxic condition including the production of ROS. Epigallocatechin gallate (EGCG). a green tea polyphenol, has diverse pharmacologial activities in cell growth and death. This study was aimed to investigate the apoptotic mechanism by CoCl_(2) and effects of EGCG on CoCl_(2)-induced apoptosis in PC12 cells. Administration of CoCl_(2) decreased cell survival in dose- and time-dependent manners and induced genomic DNA fragmentation. Treatment with 100 µM EGCG for 30 min before PC12 cells were exposed to 150 µM CoCl_(2), being resulted in the cell viability and DNA fragmentation being rescued. CoCl_(2) caused morphologic changes such as cell swelling and condensed nuclei, whereas EGCG attenuated morphologic changes by CoCl_(2). EGCG suppressed the apoptotic peak and a loss of Δψ_(m) induced by CoCl_(2). CoCl_(2) decreased Bcl-2 expression but Bax expression was not changed in CoCl_(2)-treated cells. EGCG attenuated the Bcl-2 underexpression by CoCl_(2). CoCl_(2) augumented the cytochrome c release from mitochondria into cytoplasm and increased caspase-8, -9 and caspase-3 activity, a marker of the apoptotic executing stage. EGCG ameliorated the incruement in caspase-8, -9 and -3 activity, and cytochrome c release by CoCl_(2). NAC (N-acetyl-cysteine), a scavenger of ROS, attenuated CoCl_(2)-induced apoptosis in consistent with those of EGCG. These results suggest, that CoCl_(2) induces apoptotic cell death through both mitochondria- and death receptor-dependent pathway and EGCG has neuroprotective effects against CoCl_(2)-induced apoptosis in PC12 cells. 신경세포자멸사는 저산소 및 허혈환경에서 일어나며 이러한 세포죽은 reactive oxidant species (ROS) 생성을 동반함이 알려져있다. 그러나, 저산소 및 허혈환경에서 일어나는 세포자멸사의 기전 및 그 치료방법은 아직 정립되어 있지 않다. CoCl_(2)는 ROS를 생성하는 등 저산소환경과 유사한 조건을 초래하는 것으로 알려져 있다. Epigallocatechin gallate (EGCG)는 녹차의 polyphenol 성분으로서 세포성장과 죽음에 다양한 약리학적 효과를 나타냄이 알려져 있다. 본 연구는 PC12 세포에서 CoCl_(2)에 의한 세포자멸사기전을 밝히고 이에 미치는 EGCG의 효과를 조사하는데 목적이 있다. Cell viability는 MTT 측정으로 조사되었고, DNA fragmentation은 DNA laddering으로 조사되었다. Bcl-2와 Bax발현 정도는 RT-PCR로, caspase-3와 -9의 활성은 spectrophotometer, caspase-8의 활성은 flow cytometry에 의해 측정되었다. 미토콘드리아에서 세포질로 분비된 cytochrome c는 western blot으로, 분해된 DNA 양과 미토콘드리아 세포막전위 (Δψ_(m))는 FACScan으로 조사되었다. CoCl_(2)투여로 PC12 세포수는 용량 및 시간 의존형태로 감소하였고, genomic DNA fragmentation이 발생하였다. CoCl_(2)투여로 야기된 cell viability의 감소와 DNA fragmentation은 EGCG 전처치에 의해 억제되었다. CoCl_(2)은 세포용적팽창과 condensed nuclei 같은 형태적 변화를 일으켰으며, apoptotic peak, Δψ_(m)감소 및 cytochrome c 유리를 야기하였다. EGCG는 CoCl_(2)에 의한 세포형태변화, apoptotic peak, Δψ_(m)소실 및 cytochrome c 유리를 억제시켰다. CoCl_(2)는 Bcl-2 발현을 감소시켰지만, Bax 발현에는 영향을 미치지 않았다. EGCG는 CoCl_(2)에 의해 야기된 Bcl-2 발현 감소를 억제시켰다. CoCl_(2)는 caspase-3, -8, 그리고 -9의 활성을 증가시켰으며, EGCG는 CoCl_(2)에 의한 세포자멸사를 억제시켰다. 본 실험결과는 PC12 세포에서 CoCl_(2)가 미토콘드리아 의존 및 death receptor의존 기전으로 세포자멸사를 일으키며, EGCG는 세포자멸사기전을 억세지킴으로 신경보호기능을 가짐을 시사하였다.

Interleukin-2가 Mouse 자궁내막의 Acid Phosphatase 활성에 미치는 영향

김원규,신규철,정호삼,이규식 한양대학교 의과대학 1989 한양의대 학술지 Vol.9 No.2

Interleukin-2, a kind of lymphokine produced by the T-lymphocyte stimulated by antigen or mitogen, has been studied intensively with the development of immuno-oncology and chemoimmunotherapy of the malignancy. Interleukin-2 is known to exert on antitumor effect by stimulating the proliferation of helper and cytotoxic T lymphocytes, augmenting the cytolytic activities of the cytotoxic T lymphocyte and differentiating a precursor cell into a lymphokine-activated killer cell in vivo. Though interleukin-2 suppresses the metastasis of the malignant tumor into the adjacent organs, it develops many side effects including fever, chills, malaise, mental confusion, diarrhea, vomiting and oliguria. Interleukin-2 also causes pulmonary, hepatic and renal toxicities and, especially, increase of capillary permeability with weight gain, pulmonary edema and ascites. In this experiment, the author studied the effect of interleukin-2 on the mouse endometrium, observing histochemically the changes in the activity of acid phosphatase following the estrous cycle. Female, DDY strain, weighing 20gm and exhibiting normal estrous cycle, were used as experimental animals. The estrous cycle was recognized by the vaginal smear. The experimental animals were treated with 2 million units of interleukin-2/0.2ml per Kg of body weight and with 0.2ml of normal saline per Kg of body weight in the control groups for 5 days. And animals were sacrificed 5th day after administration of interleukin-2. The specimens obtained from the corpus uteri were fixed in 10% neutral buffered formalin solution for 24 hours at 4℃ and sliced at 14μm thickness in a frozen cryostat. The activity of acid phosphatase was observed by the Gomori's method for histochemical study. The result obtained was as follows: The acid phosphatase activity was increased at estrus and decreased at diestrus in interleukin-2 treated group. There was, however, no marked differences in acid phosphatase activity of the proestrus and metestrus between control and interleukin-2 treated groups. Consequently, it was suggested that interleukin-2 develop the changes of the acid phosphatase of the endometrium of the mouse.

전략적 공급관계에서의 관리통제시스템이 협력 및 성과에 미치는 영향

김규철(Kim Kyu-chul),최종민(Choe Jong-min) 한국국제회계학회 2018 국제회계연구 Vol.0 No.80

본 연구는 전략적 공급관계에서 성과평가시스템과 사회화프로세스가 협력(4차원: 정보공유/문제해결, 변화에 적응하려는 의지, 권력의 사용 억제)을 통해 어떻게 관계성과를 향상시키는지를 실증적으로 연구하였다. 회귀분석 결과, 성과평가시스템과 사회화프로세스는 관계성과에 유의한 양의 영향이 미친다. 성과평가시스템(재무적)에서는 권력의 사용 억제에 대해서만 유의한 양의 영향을 미쳤고, 성과평가시스템(비재무적)과 사회화프로세스에서는 ‘정보공유/문제해결’에서만 유의한 양의 영향을 미쳤다. 그리고 협력 4차원중 ‘정보공유/문제해결’과 ‘변화에 적응하려는 의지’만 관계성과에 유의한 양의 영향을 미친다. 관계 성과에 대한 간접영향 분석에서는 성과평가시스템(비재무적)은 ‘권력의 사용 억제’를 통한 관계 성과에 대해서만 유의한 양의 영향이 미치는 것으로 나타났다. 협력 4차원에 유의적인 양의 영향은 관련 기업들과의 서로 합심해서 정보 공유하고, 공동으로 함께 해결하고 노력할 때 구매기업과 공급기업에 서로 만족하는 관계 성과를 가능하게 한다는 의미이다. 성과평가시스템과 사회화프로세스로 실증 연구를 해 본 결과, 협력(4차원)을 통한 관계 성과 향상을 위해서는 성과평가시스템과 사회화프로세스를 병행해서 사용하는 것이 기업의 관리 통제에 효과적인 방안임을 의미한다. 또 사회화프로세스가 관계성과와 관련이 있음을 나타낸다. This study empirically investigated how PMS and Socialization process improve Relationship performance through Cooperation(4dimensions: Information sharing/Problem solving, Willingness to adapt to changes, Restraint from use of power) in Strategic supply relationships. The result of the regression analysis showed that PMS and Socialization process have a positive impact on the Relationship performance. The PMS(financial) had olny a significant a positive impect on the Restraint from use of power, and the PMS(non-financial) and socialization process had only a significant a positive impect on ‘Information sharing/Problem solving". In addition, ‘Information sharing/Problem solving" and ‘Willingness to adapt to change" among the 4 dimensions of Cooperation have only a significant a positive impact on Relationship performance. In the indirect effect analysis of Relationship performance, PMS(nonfinancial) have only a significant a positive impact on the relationship performance through ‘Restraint from use of power". Significant impacts on the 4dimensions of Cooperation mean that it is possible to achieve satisfactory Relationship Performance between the purchasing and supplying companies when sharing information with the related companies sharing and working together. As a result of empirical studies with the PMS and the Socialization process, it means that the use of the PMS and the Socialization process together is an effective way to control the enterprise in order to improve the Relationship performance through Cooperation(4-dimensions). It also indicates that the Socialization process is related to Relationship performance.

흉선종 제거후에 발생한 재생 불량성 빈혈 : 증례보고

전원선,이상철,김현정,배상병,김찬규,이남수,박노진,이규택,박성규,홍대식,박희숙,원종호 순천향의학연구소 2007 Journal of Soonchunhyang Medical Science Vol.13 No.2

Thymoma is associated with myasthenia gravis, Pure red cell aplasia, and autoimmune diseases such as autoimmune hemolytic anemia, aplastic anemia, and hypogammaglobulinemia. It's association with aplastic anemia is rare and aplastic anemia appearing after surgical removal of thymoma is especially rare. The authors hereby report a case of aplastic anemia occuring in a patient who was diagnosed with thymoma and myasthenia gravis and had the tumor surgically removed. The patient was treated with anti-thymocyte globulin, cyclosporin, and prednisolone, and showed partial remission with hematologic improvements after 12 months.

지속성 외래 복막투석 환자에서 염증 지표와 고해상도 B 모드 경동맥 초음파 소견의 연관성에 관한 연구

송현용,송영수,안철우,강신욱,최규헌,하성규,이현철,이호영,한대석 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.2

배 경 : 저알부민혈증 및 C-반응 단백(C-reactive protein, CRP)이 증가된 지속성 외래 복막투석 환자의 사망률이 높은 정확한 이유는 밝혀져 않다. 혈액투석 환자에서처럼 복막투석 환자에서 저알부민혈증 및 염증반응이 심혈관계 질환 위험인자 인지 알아보고자 하였다. 방 법: 비당뇨병성 말기 신부전, 지속성 외래 복막투석 환자 93명의 심혈관계 질환 위험인자들과 복막 투과 특성 및 알부민, CRP 등의 염증지표에 따라 고해상도 초음파를 이용한 경동맥내막-중간막 두께, 내막-중간막 면적, 플라크의 유무 등을 단면적으로 비교하였다. 결 과: 복막 투과 특성은 고투과막군 14명, 중간 고투과막군 38명, 중간 저투과막군 31명, 저투과막군 10명으로 각 군간의 경동맥 내막-중간막 두께, 플라크 유무 및 내막-중간막 면적의 차이는 없었다. 기존 관상동맥 질환 환자는 8예(8.6%)로 내막-중간막 두께는 관상동맥 질환이 없는 환자보다 증가되었고(0.79±0.21 vs 0.60±0.11 mm, p<0.05) 플라크 형성은 더 많이 보이나 통계적 유의성은 없었다(75.0 vs 63.5%, p>0.05). 혈청 알부민치와 경동맥 내막-중간막 면적은 역상관관계를 보였다(r=-0.27, p<0.05). 저알부민혈증 환자(혈청 알부민 <3.5 g/dL)는 정상 알부민혈증 환자보다 내막-중간막 두께가 증가되었고(0.67±0.15 vs 0.61±0.12 mm, p<0.05), 플라크도 유의하게 많았다(68.0 vs 55.8%, p<0.05). 혈청 CRP치와 경동맥 내막-중간막 면적은 양의 상관관계가 있었으며(r=0.21, p<0.05) CRP가 높은 환자(≥0.8 mg/dL, n=18)에서플라크가 많이 관찰되었으나(65.8 vs 50.0%, p<0.05) 경동맥 내막-중간막 두께 및 내막-중간막면적은 양군간에 차이가 없었다. 다변량 회귀 분석상 경동맥 내막-중간막 두께에 영향을 미치는 인자는 고령, 높은 CRP, 기존 심혈관계질환, 낮은 혈청 알부민 순이였다. 결 론 : 복막투석 환자에서 고해상도 초음파를 이용한 경동맥 내막-중간막 두께 측정은 심혈관계 질환 유무 예측의 비침습적 검사가 될 수 있고 저알부민혈증 및 높은 CRP과 같은 만성염증 지속상태가 복막투석 환자에서 죽상 동맥 경화증의 발생을 증가시킨다고 가정할 수 있다. Background : Continuous ambulatory peritoneal dialysis(CAPD) patients with low albumin(LA) and signs of inflammation reflected by increased C-reactive protein(CRP) level have an increased mortality, but the mechanism of this phenomenon is not clear yet. Methods : To answer whether LA and inflammation also enhance cardiovascular risk in CAPD patients, we performed cross sectional study measuring carotid artery intima-media thickness(IMT), calculated intima-media area(cIM area) and the presence of plaque by high-resolution B-mode ultrasonography in 93 non-diabetic CAPD patients. Results : Patients with coronary artery disease (CAD, n=8) had significantly increased IMT(0.79± 0.21 mm vs. 0.60±0.11 mm, p<0.05) and higher prevalence of carotid plaques(75.0% vs. 63.5%) compared to the non-CAD patients. Significant inverse correlation was observed between serum albumin (SA) level and cIM area(r=-0.27, p<0.05). Those patients with LA(SA <3.5 g/dL) had significantly increased IMT compared to non-LA patients(0.67±0.15 mm vs 0.61±0.12 mm, p<0.05). Prevalence of carotid plaques was also significantly higher in LA patients (68.0% vs. 55.8%, p<0.05). CRP level revealed a significant positive correlation with cIM area(r=0.21, p< 0.05). Patients with high CRP(≥0.8 mg/dL, n=18) had higher prevalence of carotid plaques (65.8% vs. 50.0%, p<0.05) compared to those patients with CRP <0.8 mg/dL, but IMT and cIMT area were not different. By multivariate logistic regression analysis, old age, high CRP, history of CAD and low SA were the independent risk factors affecting IMT. Conclusion : Our study strongly suggests that low albumin and chronic inflammatory state of CAPD patients could be associated with increasing atherosclerotic cardiovascular disease. (Korean J Nephrol 2002;21(2):285-294)

신사구체 경변증을 나타내는 FGS/NgaKist mice 간장에서 사이토크롬 P4501A1/2 단백질 발현

정규식,현병화,이철호,박상준,최양규,류시윤,이차수,민병길 대한해부학회 1997 Anatomy & Cell Biology Vol.30 No.2

규제순응의 제고 : Policy Alternatives for better Regulation

李哲圭 水原大學校 2005 論文集 Vol.23 No.-

The institutional framework for regulatory reform was completed in 1998 as the government enacted the Government Regulation Act and established the Regulatory Reform Committee under the President. As a result, enormous quantifies of regulation have been reduced oyer the last seven years since its establishment. Most of People and business enterprises, however, disagree with the result. According to recent survey. 79% of enterprises answered that government regulation had not been reformed or changed. Some of them even believe it had been increased. The huge evaluation gaps exist between Government and business enterprises. Government insists enduring regulations are inevitable but business institutions do not endorse. The former believes that only the reasonable regulatory measures are being implemented, but the latter not. Government, under these circumstances, should make an effort to improve the degree of regulatory compliance. The Regulatory Impact Analysis has to be thoroughly fulfilled, if necessary. Moreover the regulation makers should consider other different points of views. including those of the regulated institutions. A regulation which is not willingly complied. should be withdrawn.

하천 및 항만에서의 수중구조물의 보수 및 유지관리에 관한 연구

서규우,신병철 동의대학교 산업기술개발연구소 2001 産業技術硏究誌 Vol.15 No.-

The purpose of this study are repair and maintenance management of submerged structures at river and harbor. Watertight concrete construction keeping water in for water retaining structures. For examples, dams, reservoirs and water tanks, water way, water gate, embankment, waste water treatment plants, sewers and pipelines. Watertight concrete construction keeping water out for waterproof facilities. Internally applied protective coatings for additional requirements of water and liquid retaining structures. Concrete erosion due to acidic environment and swelling from sulphate are applied for protective coating as infiltration polymer mortar, ceramic concoat and sika.

간 낭종을 동반한 성인 다낭신 환자에서 간 낭종의 감염 1예

정규성,서정철,주기산,김원식,임영국,정종훈,문철웅 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1

Adult polycystic kidney disease(APKD) usually accompanies with renal and extrarenal manifestations and liver cysts are present in aproximately 30% of patients with APKD. Kidney cysts often accompany with hemorrhage, rupture, infection, however, liver cysts are usually asymptomatic and do not impair liver function. Rare complication of liver cysts in patient with APKD include portal hypertension with bleeding esophageal varices and hepatic failure with encephalopathy. But, in APKD patient, infection of liver cysts without infection of renal cysts is extremely rare. We report a case of infected liver cyst in a patient with APKD who has complained generalized abdominal pain and high fever which was confirmed by ultrasound and aspiration. The patient was treated successfully with repeated aspiration and systemic antibiotics.

回韓圓板生物膜法으로 都市下水 處理時 圓板表面積과 附着汚尼量에 대하여

李正奎,朴永圭,李哲熙,徐裕德 嶺南大學校 環境問題硏究所 1987 環境硏究 Vol.6 No.2

This paper discussed on the sruface area of disc and the amount of attached biomass by kinetics cinstant at each stage, for treating sewage with rotating biological contactor (RBC). The results are shown as follows; 1. It was found in all experiment that substrate removal could bo divided into two phase. Over 60% of substratre was removed in the first phase. The first phase consisted of the first stage. The remaining substrate removed in the second phase consisting of the second, third and fourth stages. Since the majority of the substrate was removed in the fitst stage and very little in the remaining stages, reducing the distance between the disc in the second phase was necessary for better efficiency in RBC design. 2. The determined saturation constant (㎏) and area capacity constant (p) for calculation of the surface area of disc were ; 69.0, 70.4 at first stage, 88.0, 74.6 at second stage and 137.0, 82.0 ar third and fourth stages, respectively. 3. Thickness of the attached biomass (film) was between 0.34mm and 3.3mm, and the percentages of contained water in the film was approximately 97%. It was also observed that the maximun value of the dry sludge index (DSI) was almost unchanged beyond the effluent concentration of 60㎎/ℓ.