생약제제인 이공산(異功散)의 Cisplatin 유도 신장독성 보호 및 항산화 효과

Lee, Kyung-Tae,Ahn, Kyoo-Seok,Chang, Sung-Goo,Oh, Soo-Myung,Jung, Jee-Chang 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Kyung-Tae Lee, Kyoo-Seok Ahn¹, Sung-Goo Chang², Soo-Myung Oh²and Jee-Chang Jung²¹College of Pharmacy, ²Oriental Medicine and ³College of Medicine, and East-West Medical Reserch Institute, Kyung Hee University, Seoul, Korea. Preventive and Antioxidative Effects of Crude Drug Preparation(E-kong-san) on Cisplatin Induced Nephrotoxicity. Proceedings of International Symposium on East-West Medicine, Seoul. 201-211, 1999. -Nephroprotective effects of a crude drug-prearation (E-kong-san) were determined from cisplatin on rabbit kidney proximal tubule and human renal cortical cells by MTT assays and sustained glucose consumption on ciplatin-induced human renal cortical tissue. Levels of creatinine and blood ure nitrogen(BUN) in serum after administration of cisplatin(0.75mg/kg,i.p.) to E-kong-san(0.75g/kg/day,p.o.)-pretreated rats were markedly lower compared to those of cisplatin-treated rats. Moreover, the administration of E-kong-san significantly inhibited the loss of body weight of cisplatin injected rts. These findings suggest that E-kong-san on the cisplatin induced nephrotoxicity led us to investigate whether the effect of this water extract was a result of anti-oxidation. E-kong-san showed strong free radical scavenger activities on 1,1-dipheny1-2picrylhydrazil(DPPH) radical and xanthine/xanthine oxidase(XOD) generated superoxide anion radical(O2-). We further studied the effects of E-kong-san on lipid peroxidation in rat liver microsomes induced by enzymatic and nonenzymatic methods. E-kong-san exhibited significant inhibition on both ascorbic acid/Fe2+and ADP/NADPH/Fe3+ induced lipid peroxidation in rat liver microsomes. Based on these results, we suggest that-E-kong-san attenuate the cisplatin induced cytotoxicity and its mechanism can be eplained by antioxidant.

계열별 고등학생집단의 뇌기능특성화 경향에 대한 연구

윤규태,최기수,안의태,고정식,양남길,박경호 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.2

본 연구에서는 계열에 따른 고등학생들의 대뇌반구기능의 특성화 또는 편재화의 경향을 알아보고, 전공분야에 대한 적응력과의 상관관계를 검토하고자 했다. 일반고등학교 인문계열 학생 359명, 자연계열 학생 371명, 음악과 미술전공 학생을 포함한 예능계열 196명, 체육특기자를 포함한 체육계열 학생 151명 등 1,077명을 대상으로 각 계열별, 남녀별, 성적별 고등학생 집단들의 대뇌반구의 특성화, 편재화 경향을 시나가와(품川)질문지법을 이용하여 분석하였으며, 다음과 같은 결과를 얻었다. 1. 인문계열 고등학생의 뇌기능의 편재화 경향은 우뇌형 54.3%, 양쪽뇌형 32.6% 및 좌뇌형 13.1%였다. 남녀별로는 남학생은 우뇌형 62.9% 양쪽뇌형 27.9% 및 좌뇌형 9.5%로 나타났으며, 여학생은 각각 43.7%, 38.6% 및 17.7%로서 여학생이 남학생에 비하여 죄뇌화 경향을 보였다. 2. 자연계열 고등학생의 경우는 우뇌형 56.9%, 양쪽뇌형 29.9% 및 좌뇌형 13.2%로서 인문계열 고등학생과 비슷한 분포를 보였다 이를 남녀별로 구별하여 보면 남학생은 62.2%, 27.1% 및 10.2%, 여학생은 51.5%, 32.5%및 16.0%의 분포를 보여, 역시 여학생의 좌뇌화 경향이 뚜렷했다. 3. 예능계열 고등학생의 경우는 우뇌형 56.1%, 양쪽뇌형 35.2% 및 좌뇌형 8.7%의 분포를 보였으며, 이를 전공별로 세분해서 살펴보면 음악전공학생의 경우 53.7%, 35.5% 및 10.7%로서 전체 평균과 거의 비슷한 분포를 보였으나, 미술전공학생의 경우 60.0%, 34.7% 및 5.3%로서 , 특히 좌뇌형의 경우 음악전공에 비해 절반수준에도 못 미치는 경향을 보였다. 4. 체능계열 고등학생 집단의 경우 우뇌형 50.3%, 양쪽뇌형 37.7% 및 좌뇌형 11.9% 분포를 보였다. 5. 성적별로 보면 일반고등학교 학생 가운데 성적 우수 집단은 뚜렷하게 좌뇌형 경향(25.6% 및 25.0%)을 보였다. 6. 예능계열의 우수집단은 양쪽뇌형의 분포비율(50.0% 및 44.4%)이 특히 높았다. 7. 체육계열의 우수집단은 좌뇌형 분포(21.6%)가 높았다. According to the advanced study on differential theory of brain function, the left hemispheric functions are concerned more on the verbal, logical, sequential and arithmetic abilities, whereas the right hemisphere has characteristics of non-lingual, general, creative and aesthetic abilities. The authors compared the tendency of hemispheric specialization in high school students with different educational condition and facilities. Sinakawa's questionnaire method was the instrument for this study. The results obtained were as follows: Among 1,077 of whole subjects, right hemispheric dominant type was 55.0% (59.1% in male, 51.0% in female), bilateral type was 32.9% (10.6% in male, 35.1% in female) and left hemispheric dominant type was 12.2% (10.4% in male, 13.9% in female). 1. Of the 359 subjects in the field of humanity science, right hemispheric dominant type was 54.3% (62.9% in male, 43.7% in female), bilateral type was 32.6% (27.9% in male, 38.6% in female) and left hemispheric dominant type was 13.1% (9.5% in male, 17.7% in female). 2. Of the 371 subjects in the field of natural science, right hemispheric dominant type was 56.9% (62.2% in male, 51.5% in female), bilateral type was 29.9% (27.1% in male, 32.5% in female) and left hemispheric dominant type was 13.2% (10.2% in male, 16.0% in female). 3. Of the 196 art school students, 56.1% was regraded as right hemispheric dominant type, students majoring music was 53.7%, 35.5% and 10.7%, respectively, whereas the frequency for students majoring find art was 60.0%, 34.7% and 5.3%. 4. In students majoring physical education, 50.3% were shown in right hemispheric dominant type, 37.7% were bilateral type and 11.9% were left hemispheric dominant type. 5. Laterality patterns of students who adapt more successfully to their specific studying fields show following characteristics; Successful students of general high school show pronounced left shift in their laterality pattern, whereas successful students of art school exhibit more bilateral type. And successful students of physical education school show left shift in their laterality patterns.

단세포군 항체를 이용한 Chlamydia trachomatis의 면역형 결정

윤규석,김덕언,최태열 대한감염학회 1993 감염 Vol.25 No.1

An immunotyping of 27 strains of Chlamydia trachomatis isolated from patients with non-gonococcal urethritis or pelvic inflammatory diseases in Korea was achieved in dot-enzyme linked immunosorbent assay (dot-ELISA) with monoclonal antibodies. Monoclonal antibodies were produced with standard techniques by immunization of Balb/c mice and fusion with SP 2/0 myeloma cell. Seven type-specific (D,E,F,H,I,J,L₂), 2 subspecies-specific (B-complex, C-complex) and species-specific (HMC 1-1) monoclonal antibodies were used for immunotyping. Immunotyping of 12 control strains and 27 clinical strains isolated in Korea was studied by using dot-ELISA. Species-specific (HMC 1-1) monoclonal antibody reacted with all control strains and 27 isolated. Subspecies-specific (B-complex) monoclonal antibody reacted with B/HAR-36, Ba/Aphach-2, D/UW-3Cx, E/Bour, LGV type Ⅰ/440, LGV type Ⅱ/CDC control strains and 19 isolates. Type-specific monoclonal antibody of D was reacted with D/UW-3/Cx control strain and 10 isolates. E type-specific monoclonal antibody racted with E/Bour control strain and 6 isolates. F type-specific monoclonal antibody reacted with 5 isolates. Three isolates which racted with subspecies-specific monoclonal antibody didn't react with any type-specific monoclonal antibodies. Subspecies-specific (C-complex) monoclonal antibody reated with A/HAR-13, C/CDC, H/UW-43/Cx, J/UW-36/Cx control strains and 2 clinical isolates, but the isolates did not react with any type-specific monoclonal antibodies. One of 27 clinical isolates reacted with any type-specific monoclonal antibodies. One of 27 isolates reacted with species-specific (HMC 1-1) monoclonal antibody didn't react with any other subspecies-and type-specific monoclonal antibodies. In conclusion, the major immunotypes of C.trachomatis from urogenital system in Korea were D, E and F, and dot-ELISA with monoclonal antibody may contribute for immunotyping as a simple and specific technique.

디밍 안정기를 이용한 형광등의 퍼지 제어기 설계

崔洪圭,全光鎬,姜太垠,崔琁雄 홍익대학교 산업기술연구소 1997 産業技術 Vol.7 No.-

This paper proposes Dimming Ballast illumination control method using fuzzy reasoning the proposed method has the following effects. First the power saving above 50% is accompanied by the automatic control of the illumination and the choice of a turn-on or -off of a fluorescent lamps that is accompanied by the number of person and working time and weather Second the maintainment of the best illumination in spite of time and weather variation. Third More simple establishment and low than BAS(Building Automation System) because need not main control unit or communication cable

유도 전동기의 동특성 개선을 위한 퍼지 제어기 설계

최홍규,전광호,최병숙,강태은 홍익대학교 산업기술연구소 1998 産業技術 Vol.8 No.-

The conventional PI control that are used in the control loop for outer speed of the induction motor, also has difficulty dealing with the dynamic speed tracking parameter variation and the load disturbance. As a result, the motion control system operates with a poor performance in the motor's low speed range. In this dissertation a novel speed control system that implements and fuzzy logic controller(FLC) is proposed. The proposed FLC used the speed error its rate of change and the drive current as three fuzzy variables. The real command output current is chosen by the dufuzzification of the fuzzy output extracted from the fuzzy control rules. The flux and the torque of the induction motor are estimated using the dynamic model with an excellent dynamic response over a wide speed range and with the rotor flux field-oriented scheme of the decoupling characteristics. The performance of this control system is substantiated by computer simulations.

퍼지 논리 제어기를 이용한 유도 전동기 전류 제어에 대한 연구

崔洪圭,全光鎬,姜泰垠 홍익대학교 산업기술연구소 1995 産業技術 Vol.5 No.-

In this paper, we propose the control method of an inverter-fed induction motor using Fuzzy Logic Controller. The FLC chooses the switching states based on a set of fuzzy variables. Error in flux component magnitude of stator current error in torque component magnitude of stator current and flux position are used as fuzzy state variables. Fuzzy rules are determined by observing the vector diagram of flux and currents. The proposed controller shows a good dynamic speed response and decoupling characteristics. Moreover, since the rotor parameter has no effects on the performance the controller and the fuzzy controller possesses highly adaptive capability the performance of fuzzy controller is quite robust and insensitive to the motor parameters and change of operation conditions

외국 자본과 국내자본에 의하여 설립된 스포츠시설 소비자의 만족도에 관한 비교연구

윤규태,원유진 순천향대학교 기초과학연구소 2004 순천향자연과학연구 논문집 Vol.10 No.2

The goal of this study is investigating the efficiency of sport centers, grasping the problems in their future improvements and suggest the direction of development by classifying the satisfaction level of the members of foreign capitalized sport centers into various factors and comparing them with that of the members of domestic capitalized sport centers. The subjects of the study were set for a group of men and women who were over 20years old, living in Seoul right now in 2004, and stratified cluster random sampling was used. In the sampling process, 300members were surveyed and 54 questionnaires were taken out of the respondents and the results are as follows. 1) For the satisfaction levels of facilities, the foreign capitalized sport centers in every area such as the safety of facilities, room temperature, humidity, brightness of light, sanitary facilities, interior design, and shower facilities. 2) For the satisfaction levels of teaching staff, the foreign capitalized sport centers were higher than the domestic capitalized sport centers in terms of teaching methods, friendliness, attitude, knowledge and ability, and relationship with members. 3) For level of satisfaction of programs and services of employees, the foreign capitalized sport centers were higher than the domestic capitalized sport centers in reorganization, variety and the level of interest in programs. It is found that the foreign capitalized sport centers do not operate shuttle and sport consumers are satisfied with the services of employees.

성견 치은에서 90% Phenol을 이용한 멜라닌 색소의 제거에 관한 연구

정태술,김영준,이영규,정현주 전남대학교 치의학연구소 2001 구강과학 Vol.13 No.4

In this present study, the healing process and the recurrence of pigmentation were evaluated clinically and histologically in accordance with the extent and the range of pigmentation after phenol was applied to remove melanin pigmentation in gingiva. Six mongrel dogs were used. The melanin pigmentation in canine gingiva were classified into slight, moderate, and severe according to the extent of pigmentation and divided into local and diffuse types according to the range. Following general and local anesthesia, 90% phenol was applied to the pigmented gingiva of the subjects with small cotton balls until the surface was etched to be whitish and was neutralized with small cotton balls soaked by 95% alcohol. The contralateral pigmented gingiva to the one treated with phenol, was treated by surgical deepithelialization. At1, 3, and 8 weeks, the treated gingiva was examined clinically and evaluated histologically following H-E stain, and HMB 45 stain for melanocyte after biopsy. In the phenol treated sites, epithelium and connective tissue healed normally and there was no pigmentation at 1 week. At 3 weeks of healing, melanin repigmentation was observed in the severe local type and moderate to severe diffuse type. In the surgically deepithelialized sites, healing was delayed, compared to phenol treated sites and the infiltration of the inflammatory cells and congestion in connective tissue was shown at 1 week. At 3 weeks, healing was completed and there was a partial melanin repigmentation. At 8 weeks of healing, the extent and the range of repigmentation were increased in both group according to the extent or range priot to depigmentation procedure. These results suggest that the removal of melanin pigmentation with 90% phenol application result in normal healing process of gingiva. However, in the severe local type and moderate to severe diffuse type, sites treated with phenol showed repigmentation at 3 week, which was earlier than surgical deepithelialized sites. Therefore it is required to select appropriate method according to initial condition of pigmentation.

고온 환경에서 최대운동부하시 음료섭취에 따른 심폐기능과 혈중 전해질농도의 변화

윤규태,이태형 순천향대학교 기초과학연구소 2000 순천향자연과학연구 논문집 Vol.6 No.1

To accomplish the purpose of this study, I divided into three groups; one group drink an ion beverage(n=5), another group drink plain water(n=5) and the other group non-drink(n=5) after 15 field hockey players who were randomly chosen at S university. And I analyzed gradually maximal exercise method by Bruce Protocol to avail Treadmill that was often used in the thermal environment(30℃) into electrolyte and responses of circulation. Statistical dealing for measuring score was comparatively analyzed to use SPSS statistical package. As a result, variation of Heart Rate hardly showed the difference in each group drink beverage, but showed considerable difference in the group non-drink in the convalescent stage. Oxyzen consumption, CO2 production and maximal oxyzen consumption per kg body weight made no difference inter-groups. Also variation of electrodyte made no difference in each group drink beverage, but showed considerable difference in the group non-drink.

연령증가에 따른 수영선수들의 최대산소섭취량에 관한 연구

윤규태 순천향대학교 기초과학연구소 1997 순천향자연과학연구 논문집 Vol.3 No.2

There were 10 numbers of elementary school player, 10 numbers of middle school player, 10 numbers of high school player, total 30 numbers of swimming player who was measured, compared and analyzed maximal oxygen uptake (VO₂max), maximal Ventilation (VE max) and maximal Breathing Frequency(BF max). Therefore different ages of player had a changeable aspects of differences among the group. Analysis of covariance was used for data analysis and the significance level was set at 0.05. The results of this study were as follows. (1)VO₂max The VO₂max appeared in order of high school player(63.52±1.32ml/kg), middle school student(59.92±1.41ml/kg), elementary school player(56.50±2.52ml/kg) group. There were significant differences among the group (p<0.001). (2) VEmax The VEmax appeared in order of high school player (134.3±2.351/min), middle school player(121.4±2.451/min), elementary school player(86.25±8.661/min) group. There were significant differences among the group. (p<0.001) (3) BFmax The BFmax appeared in order of elementary school player (55.6±3.91F/min), middle school player(53.2±3.37F/min), high school player(49.7±2.57F/min)group. There were significant differences among the group.(p<0.001)